Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-10_13.45.48 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "953c6e5afb0a5b1697ee5713a5d248ca",
"name": "M1_20170606_1546_dsRNA_O1_col.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "11:46:10"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 9036 (max 9037) in '9036'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 25.0394
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 1.92741e+009
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 550 frames Cell Events: 31 mean_circ: 0.947355
Ran for 1050 frames Cell Events: 59 mean_circ: 0.946431
Ran for 1550 frames Cell Events: 89 mean_circ: 0.946066
Ran for 2050 frames Cell Events: 123 mean_circ: 0.94914
Ran for 2550 frames Cell Events: 143 mean_circ: 0.947423
Ran for 4050 frames Cell Events: 214 mean_circ: 0.949466
Ran for 5550 frames Cell Events: 311 mean_circ: 0.951701
Ran for 7550 frames Cell Events: 446 mean_circ: 0.951163
Ran for 8550 frames Cell Events: 500 mean_circ: 0.950717
Ran for 10050 frames Cell Events: 586 mean_circ: 0.950949
Ran for 11050 frames Cell Events: 627 mean_circ: 0.950991
Ran for 11550 frames Cell Events: 651 mean_circ: 0.951007
Ran for 12050 frames Cell Events: 692 mean_circ: 0.951088
Ran for 12550 frames Cell Events: 712 mean_circ: 0.951582
Ran for 13550 frames Cell Events: 774 mean_circ: 0.951098
Ran for 14550 frames Cell Events: 833 mean_circ: 0.951077
Ran for 15050 frames Cell Events: 869 mean_circ: 0.951313
Ran for 15550 frames Cell Events: 899 mean_circ: 0.951026
Ran for 16050 frames Cell Events: 922 mean_circ: 0.951034
Ran for 16550 frames Cell Events: 942 mean_circ: 0.95078
Ran for 17550 frames Cell Events: 1007 mean_circ: 0.950962
Ran for 19050 frames Cell Events: 1109 mean_circ: 0.951169
Ran for 20050 frames Cell Events: 1136 mean_circ: 0.951169
Ran for 20550 frames Cell Events: 1161 mean_circ: 0.951043
Ran for 21050 frames Cell Events: 1176 mean_circ: 0.951023
Ran for 21550 frames Cell Events: 1204 mean_circ: 0.951279
Ran for 22550 frames Cell Events: 1266 mean_circ: 0.951351
Ran for 23050 frames Cell Events: 1303 mean_circ: 0.951164
Ran for 26050 frames Cell Events: 1473 mean_circ: 0.951285
Ran for 26550 frames Cell Events: 1509 mean_circ: 0.951296
Ran for 27050 frames Cell Events: 1523 mean_circ: 0.951206
Ran for 28050 frames Cell Events: 1594 mean_circ: 0.951149
Ran for 28550 frames Cell Events: 1617 mean_circ: 0.95113
Ran for 29550 frames Cell Events: 1685 mean_circ: 0.951122
Ran for 31550 frames Cell Events: 1797 mean_circ: 0.951245
Ran for 32050 frames Cell Events: 1826 mean_circ: 0.951142
Ran for 32550 frames Cell Events: 1848 mean_circ: 0.951099
Ran for 33550 frames Cell Events: 1917 mean_circ: 0.951216
Ran for 35550 frames Cell Events: 1977 mean_circ: 0.951197
Ran for 36050 frames Cell Events: 1997 mean_circ: 0.951187
Ran for 36550 frames Cell Events: 2017 mean_circ: 0.951058
Ran for 37050 frames Cell Events: 2041 mean_circ: 0.951065
Ran for 38550 frames Cell Events: 2133 mean_circ: 0.951178
Ran for 40050 frames Cell Events: 2199 mean_circ: 0.950944
Ran for 40550 frames Cell Events: 2222 mean_circ: 0.951014
Ran for 41550 frames Cell Events: 2294 mean_circ: 0.95118
Ran for 43050 frames Cell Events: 2418 mean_circ: 0.951222
Ran for 44050 frames Cell Events: 2479 mean_circ: 0.951434
Ran for 44550 frames Cell Events: 2513 mean_circ: 0.951399
Ran for 45050 frames Cell Events: 2554 mean_circ: 0.951406
Ran for 46050 frames Cell Events: 2619 mean_circ: 0.951201
Ran for 48050 frames Cell Events: 2731 mean_circ: 0.951422
Ran for 49550 frames Cell Events: 2833 mean_circ: 0.950978
Ran for 50050 frames Cell Events: 2877 mean_circ: 0.95101
Ran for 51550 frames Cell Events: 2935 mean_circ: 0.951119
Ran for 52550 frames Cell Events: 3011 mean_circ: 0.951124
Ran for 53050 frames Cell Events: 3037 mean_circ: 0.951091
Ran for 54550 frames Cell Events: 3095 mean_circ: 0.950981
Ran for 55550 frames Cell Events: 3153 mean_circ: 0.951023
Ran for 57550 frames Cell Events: 3264 mean_circ: 0.951187
Ran for 58550 frames Cell Events: 3309 mean_circ: 0.951182
Ran for 61050 frames Cell Events: 3459 mean_circ: 0.95131
Ran for 63550 frames Cell Events: 3606 mean_circ: 0.951499
Ran for 64050 frames Cell Events: 3621 mean_circ: 0.951492
Ran for 64550 frames Cell Events: 3652 mean_circ: 0.951417
Ran for 66050 frames Cell Events: 3736 mean_circ: 0.951379
Ran for 67550 frames Cell Events: 3832 mean_circ: 0.951166
Ran for 68550 frames Cell Events: 3904 mean_circ: 0.951074
Ran for 70550 frames Cell Events: 4027 mean_circ: 0.951063
Ran for 74050 frames Cell Events: 4265 mean_circ: 0.951089
Ran for 75550 frames Cell Events: 4361 mean_circ: 0.951015
Ran for 76050 frames Cell Events: 4394 mean_circ: 0.951022
Ran for 77550 frames Cell Events: 4482 mean_circ: 0.951003
Ran for 79050 frames Cell Events: 4585 mean_circ: 0.95101
Ran for 81050 frames Cell Events: 4710 mean_circ: 0.951035
Ran for 81550 frames Cell Events: 4727 mean_circ: 0.951065
Ran for 82050 frames Cell Events: 4744 mean_circ: 0.951076
Ran for 83050 frames Cell Events: 4816 mean_circ: 0.95108
Ran for 83550 frames Cell Events: 4840 mean_circ: 0.951084
Ran for 85050 frames Cell Events: 4904 mean_circ: 0.951049
Ran for 87550 frames Cell Events: 5044 mean_circ: 0.951063
Ran for 88050 frames Cell Events: 5062 mean_circ: 0.951117
Ran for 89550 frames Cell Events: 5173 mean_circ: 0.951229
Ran for 90550 frames Cell Events: 5213 mean_circ: 0.951239
Ran for 91050 frames Cell Events: 5242 mean_circ: 0.951257
Ran for 96050 frames Cell Events: 5568 mean_circ: 0.951354
Ran for 96550 frames Cell Events: 5588 mean_circ: 0.951382
Ran for 97050 frames Cell Events: 5624 mean_circ: 0.951397
Ran for 99050 frames Cell Events: 5741 mean_circ: 0.951465
Ran for 99550 frames Cell Events: 5761 mean_circ: 0.951406
Ran for 100050 frames Cell Events: 5792 mean_circ: 0.95141
Ran for 101050 frames Cell Events: 5845 mean_circ: 0.951403
Ran for 102550 frames Cell Events: 5967 mean_circ: 0.951416
Ran for 103050 frames Cell Events: 5992 mean_circ: 0.951373
Ran for 104050 frames Cell Events: 6056 mean_circ: 0.951285
Ran for 105050 frames Cell Events: 6114 mean_circ: 0.951278
Ran for 106050 frames Cell Events: 6156 mean_circ: 0.951256
Ran for 106550 frames Cell Events: 6176 mean_circ: 0.951257
Ran for 107550 frames Cell Events: 6228 mean_circ: 0.951256
Ran for 108050 frames Cell Events: 6250 mean_circ: 0.951233
Ran for 111050 frames Cell Events: 6469 mean_circ: 0.951099
Ran for 112550 frames Cell Events: 6573 mean_circ: 0.95109
Ran for 113550 frames Cell Events: 6629 mean_circ: 0.951058
Ran for 114050 frames Cell Events: 6659 mean_circ: 0.951048
Ran for 118050 frames Cell Events: 6900 mean_circ: 0.950966
Ran for 120050 frames Cell Events: 7030 mean_circ: 0.950902
Ran for 121550 frames Cell Events: 7117 mean_circ: 0.950947
Ran for 122050 frames Cell Events: 7137 mean_circ: 0.950995
Ran for 124050 frames Cell Events: 7253 mean_circ: 0.950986
Ran for 125050 frames Cell Events: 7332 mean_circ: 0.950904
Ran for 126050 frames Cell Events: 7396 mean_circ: 0.950964
Ran for 126550 frames Cell Events: 7418 mean_circ: 0.950962
Ran for 127050 frames Cell Events: 7442 mean_circ: 0.950981
Ran for 127550 frames Cell Events: 7471 mean_circ: 0.950919
Ran for 128050 frames Cell Events: 7507 mean_circ: 0.950932
Ran for 128550 frames Cell Events: 7537 mean_circ: 0.950923
Ran for 129050 frames Cell Events: 7569 mean_circ: 0.950904
Ran for 129550 frames Cell Events: 7597 mean_circ: 0.95088
Ran for 130050 frames Cell Events: 7648 mean_circ: 0.95088
Ran for 130550 frames Cell Events: 7681 mean_circ: 0.95088
Ran for 132050 frames Cell Events: 7779 mean_circ: 0.950925
Ran for 132550 frames Cell Events: 7798 mean_circ: 0.950946
Ran for 134050 frames Cell Events: 7898 mean_circ: 0.950863
Ran for 134550 frames Cell Events: 7914 mean_circ: 0.950869
Ran for 138050 frames Cell Events: 8134 mean_circ: 0.950839
Ran for 140050 frames Cell Events: 8251 mean_circ: 0.950896
Ran for 141050 frames Cell Events: 8305 mean_circ: 0.950922
Ran for 141550 frames Cell Events: 8329 mean_circ: 0.950906
Ran for 142550 frames Cell Events: 8395 mean_circ: 0.950937
Ran for 143050 frames Cell Events: 8418 mean_circ: 0.950937
Ran for 143550 frames Cell Events: 8442 mean_circ: 0.950935
Ran for 144050 frames Cell Events: 8463 mean_circ: 0.950906
Ran for 144550 frames Cell Events: 8489 mean_circ: 0.95085
Ran for 146050 frames Cell Events: 8575 mean_circ: 0.950826
Ran for 147050 frames Cell Events: 8633 mean_circ: 0.950795
Ran for 149050 frames Cell Events: 8782 mean_circ: 0.950809
Ran for 149550 frames Cell Events: 8828 mean_circ: 0.950809
Ran for 150550 frames Cell Events: 8905 mean_circ: 0.950724
Ran for 151550 frames Cell Events: 8967 mean_circ: 0.950721



End this measurment..



Stopping time: 1.92749e+009
****************************************
Measurement finished

Mean Circularitiy: 0.950751
Mean Area: 627.261
Mean FL-1 intensity: 4025.47
Mean FL-2 intensity: 4446.81
Mean FL-3 intensity: 0

frames processed: 149622
Total Time: 76377ms
Number of Runs (new bg img) 0
Frames collected: 152585
Skipped frames: 70091
Rescued Frames: 1757824
Samples DAQ: 38146989
Samples written: 76293800
Samples per Channel: 38146900
FPS: 1997.79

Samples per second: 499455
Expected samples/s: 500000

Events: 9037
Events per second: 118.321
jumped back (PMT): 3387
#Frames read, frequency
# 0 frames :241382
# 1 frames :50892
# 2 frames :3221
# 3 frames :927
# 4 frames :624
# 5 frames :313
# 6 frames :1319
# 7 frames :1319
# 8 frames :1119
# 9 frames :689
# 10 frames :551
# 11 frames :342
# 12 frames :272
# 13 frames :215
# 14 frames :223
# 15 frames :260
# 16 frames :206
# 17 frames :154
# 18 frames :116
# 19 frames :109
# 20 frames :81
# 21 frames :109
# 22 frames :73
# 23 frames :60
# 24 frames :53
# 25 frames :52
# 26 frames :46
# 27 frames :37
# 28 frames :28
# 29 frames :31
# 30 frames :23
# 31 frames :25
# 32 frames :23
# 33 frames :22
# 34 frames :10
# 35 frames :12
# 36 frames :12
# 37 frames :13
# 38 frames :12
# 39 frames :6
# 40 frames :10
# 41 frames :4
# 42 frames :4
# 43 frames :4
# 44 frames :1
# 45 frames :6
# 46 frames :1
# 47 frames :7
# 48 frames :3
# 49 frames :2
# 50 frames :5
# 51 frames :3
# 52 frames :1
# 53 frames :4
# 54 frames :2
# 55 frames :1
# 56 frames :2
# 57 frames :2
# 58 frames :2
# 62 frames :2
# 73 frames :1
# 77 frames :1
# 83 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.04
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 6
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 25.0394
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 6
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 300
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0