Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-10_16.00.49 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "11bb06172eb414dd3c22ae02e6441b46",
"name": "M1_20170611_1413_dsRNA_O1_col.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "14:01:10"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8168 (max 8169) in '8168'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 24.8385
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 2.35383e+009
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 550 frames Cell Events: 20 mean_circ: 0.95291
Ran for 2550 frames Cell Events: 71 mean_circ: 0.942193
Ran for 3050 frames Cell Events: 83 mean_circ: 0.943714
Ran for 4050 frames Cell Events: 119 mean_circ: 0.943249
Ran for 4550 frames Cell Events: 137 mean_circ: 0.942586
Ran for 5550 frames Cell Events: 160 mean_circ: 0.942662
Ran for 6050 frames Cell Events: 169 mean_circ: 0.942768
Ran for 6550 frames Cell Events: 184 mean_circ: 0.943109
Ran for 7050 frames Cell Events: 195 mean_circ: 0.94332
Ran for 7550 frames Cell Events: 213 mean_circ: 0.942129
Ran for 8050 frames Cell Events: 224 mean_circ: 0.943123
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Ran for 10050 frames Cell Events: 272 mean_circ: 0.941756
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Ran for 12550 frames Cell Events: 344 mean_circ: 0.943236
Ran for 13050 frames Cell Events: 359 mean_circ: 0.943305
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Ran for 15050 frames Cell Events: 421 mean_circ: 0.944102
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Ran for 63550 frames Cell Events: 1948 mean_circ: 0.947105
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Ran for 76050 frames Cell Events: 2374 mean_circ: 0.947024
Ran for 76550 frames Cell Events: 2383 mean_circ: 0.946853
Ran for 77550 frames Cell Events: 2418 mean_circ: 0.946927
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Ran for 85050 frames Cell Events: 2650 mean_circ: 0.946968
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Ran for 86550 frames Cell Events: 2708 mean_circ: 0.947083
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Ran for 88050 frames Cell Events: 2763 mean_circ: 0.947103
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Ran for 91550 frames Cell Events: 2868 mean_circ: 0.947096
Ran for 92050 frames Cell Events: 2882 mean_circ: 0.947147
Ran for 92550 frames Cell Events: 2898 mean_circ: 0.947183
Ran for 93050 frames Cell Events: 2907 mean_circ: 0.94716
Ran for 94050 frames Cell Events: 2938 mean_circ: 0.947277
Ran for 94550 frames Cell Events: 2954 mean_circ: 0.947291
Ran for 95050 frames Cell Events: 2977 mean_circ: 0.947332
Ran for 95550 frames Cell Events: 2994 mean_circ: 0.947386
Ran for 96050 frames Cell Events: 3005 mean_circ: 0.947393
Ran for 97050 frames Cell Events: 3046 mean_circ: 0.947356
Ran for 99050 frames Cell Events: 3116 mean_circ: 0.947428
Ran for 100050 frames Cell Events: 3152 mean_circ: 0.947488
Ran for 101050 frames Cell Events: 3197 mean_circ: 0.947449
Ran for 101550 frames Cell Events: 3212 mean_circ: 0.947484
Ran for 102050 frames Cell Events: 3230 mean_circ: 0.94749
Ran for 102550 frames Cell Events: 3247 mean_circ: 0.947458
Ran for 103050 frames Cell Events: 3260 mean_circ: 0.94748
Ran for 103550 frames Cell Events: 3274 mean_circ: 0.947496
Ran for 104050 frames Cell Events: 3284 mean_circ: 0.9475
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Ran for 105550 frames Cell Events: 3356 mean_circ: 0.947467
Ran for 106550 frames Cell Events: 3384 mean_circ: 0.947428
Ran for 108050 frames Cell Events: 3439 mean_circ: 0.947445
Ran for 109550 frames Cell Events: 3479 mean_circ: 0.947481
Ran for 110050 frames Cell Events: 3499 mean_circ: 0.947523
Ran for 111050 frames Cell Events: 3527 mean_circ: 0.947596
Ran for 111550 frames Cell Events: 3546 mean_circ: 0.947618
Ran for 112050 frames Cell Events: 3567 mean_circ: 0.947715
Ran for 115050 frames Cell Events: 3662 mean_circ: 0.94743
Ran for 115550 frames Cell Events: 3677 mean_circ: 0.947494
Ran for 116050 frames Cell Events: 3693 mean_circ: 0.947501
Ran for 117050 frames Cell Events: 3726 mean_circ: 0.947612
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Ran for 120050 frames Cell Events: 3840 mean_circ: 0.947742
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Ran for 121550 frames Cell Events: 3888 mean_circ: 0.94774
Ran for 122050 frames Cell Events: 3905 mean_circ: 0.947753
Ran for 122550 frames Cell Events: 3920 mean_circ: 0.947691
Ran for 123550 frames Cell Events: 3956 mean_circ: 0.947656
Ran for 124550 frames Cell Events: 3984 mean_circ: 0.9476
Ran for 126050 frames Cell Events: 4033 mean_circ: 0.947458
Ran for 126550 frames Cell Events: 4058 mean_circ: 0.947477
Ran for 127050 frames Cell Events: 4072 mean_circ: 0.947453
Ran for 127550 frames Cell Events: 4087 mean_circ: 0.947447
Ran for 128050 frames Cell Events: 4101 mean_circ: 0.947408
Ran for 129050 frames Cell Events: 4135 mean_circ: 0.947466
Ran for 130050 frames Cell Events: 4162 mean_circ: 0.947453
Ran for 130550 frames Cell Events: 4187 mean_circ: 0.94755
Ran for 131050 frames Cell Events: 4205 mean_circ: 0.947563
Ran for 131550 frames Cell Events: 4218 mean_circ: 0.947471
Ran for 133050 frames Cell Events: 4270 mean_circ: 0.947497
Ran for 133550 frames Cell Events: 4284 mean_circ: 0.947462
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Ran for 138050 frames Cell Events: 4458 mean_circ: 0.947562
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Ran for 140050 frames Cell Events: 4520 mean_circ: 0.947564
Ran for 141050 frames Cell Events: 4569 mean_circ: 0.947529
Ran for 141550 frames Cell Events: 4579 mean_circ: 0.947534
Ran for 142050 frames Cell Events: 4599 mean_circ: 0.94753
Ran for 143050 frames Cell Events: 4630 mean_circ: 0.947593
Ran for 144050 frames Cell Events: 4660 mean_circ: 0.947666
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Ran for 146550 frames Cell Events: 4740 mean_circ: 0.947646
Ran for 147050 frames Cell Events: 4750 mean_circ: 0.947644
Ran for 147550 frames Cell Events: 4769 mean_circ: 0.947634
Ran for 148050 frames Cell Events: 4786 mean_circ: 0.947632
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Ran for 151050 frames Cell Events: 4878 mean_circ: 0.947598
Ran for 152050 frames Cell Events: 4916 mean_circ: 0.947601
Ran for 152550 frames Cell Events: 4940 mean_circ: 0.947595
Ran for 153050 frames Cell Events: 4954 mean_circ: 0.947561
Ran for 153550 frames Cell Events: 4967 mean_circ: 0.947554
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Ran for 181550 frames Cell Events: 5922 mean_circ: 0.947511
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Ran for 200550 frames Cell Events: 6572 mean_circ: 0.947313
Ran for 201550 frames Cell Events: 6619 mean_circ: 0.947319
Ran for 202050 frames Cell Events: 6639 mean_circ: 0.947295
Ran for 204050 frames Cell Events: 6713 mean_circ: 0.947336
Ran for 204550 frames Cell Events: 6725 mean_circ: 0.947314
Ran for 205050 frames Cell Events: 6738 mean_circ: 0.947336
Ran for 206050 frames Cell Events: 6779 mean_circ: 0.947348
Ran for 206550 frames Cell Events: 6794 mean_circ: 0.947336
Ran for 207050 frames Cell Events: 6818 mean_circ: 0.947318
Ran for 207550 frames Cell Events: 6840 mean_circ: 0.947286
Ran for 208050 frames Cell Events: 6857 mean_circ: 0.947298
Ran for 208550 frames Cell Events: 6866 mean_circ: 0.947307
Ran for 209050 frames Cell Events: 6884 mean_circ: 0.947337
Ran for 209550 frames Cell Events: 6901 mean_circ: 0.947334
Ran for 210050 frames Cell Events: 6922 mean_circ: 0.947364
Ran for 210550 frames Cell Events: 6935 mean_circ: 0.947369
Ran for 211550 frames Cell Events: 6975 mean_circ: 0.947372
Ran for 212050 frames Cell Events: 6990 mean_circ: 0.94734
Ran for 212550 frames Cell Events: 7009 mean_circ: 0.947324
Ran for 213050 frames Cell Events: 7036 mean_circ: 0.947288
Ran for 214050 frames Cell Events: 7069 mean_circ: 0.94726
Ran for 215050 frames Cell Events: 7096 mean_circ: 0.947269
Ran for 217050 frames Cell Events: 7181 mean_circ: 0.947259
Ran for 218550 frames Cell Events: 7234 mean_circ: 0.94725
Ran for 219050 frames Cell Events: 7248 mean_circ: 0.947275
Ran for 219550 frames Cell Events: 7261 mean_circ: 0.947303
Ran for 220050 frames Cell Events: 7275 mean_circ: 0.947306
Ran for 220550 frames Cell Events: 7291 mean_circ: 0.947327
Ran for 221050 frames Cell Events: 7307 mean_circ: 0.947346
Ran for 222550 frames Cell Events: 7347 mean_circ: 0.947343
Ran for 223550 frames Cell Events: 7377 mean_circ: 0.947342
Ran for 224050 frames Cell Events: 7394 mean_circ: 0.947373
Ran for 224550 frames Cell Events: 7408 mean_circ: 0.947346
Ran for 225050 frames Cell Events: 7428 mean_circ: 0.947365
Ran for 227550 frames Cell Events: 7525 mean_circ: 0.947394
Ran for 228550 frames Cell Events: 7553 mean_circ: 0.947359
Ran for 229050 frames Cell Events: 7572 mean_circ: 0.94736
Ran for 229550 frames Cell Events: 7583 mean_circ: 0.947376
Ran for 230050 frames Cell Events: 7601 mean_circ: 0.947378
Ran for 230550 frames Cell Events: 7611 mean_circ: 0.947376
Ran for 231050 frames Cell Events: 7621 mean_circ: 0.947363
Ran for 232050 frames Cell Events: 7649 mean_circ: 0.947387
Ran for 232550 frames Cell Events: 7663 mean_circ: 0.947364
Ran for 233050 frames Cell Events: 7690 mean_circ: 0.94738
Ran for 233550 frames Cell Events: 7708 mean_circ: 0.947365
Ran for 234550 frames Cell Events: 7745 mean_circ: 0.947399
Ran for 235050 frames Cell Events: 7768 mean_circ: 0.947382
Ran for 235550 frames Cell Events: 7795 mean_circ: 0.947382
Ran for 236050 frames Cell Events: 7811 mean_circ: 0.947387
Ran for 236550 frames Cell Events: 7827 mean_circ: 0.947377
Ran for 237050 frames Cell Events: 7834 mean_circ: 0.947359
Ran for 238550 frames Cell Events: 7871 mean_circ: 0.947384
Ran for 239050 frames Cell Events: 7889 mean_circ: 0.947373
Ran for 240550 frames Cell Events: 7948 mean_circ: 0.947377
Ran for 241050 frames Cell Events: 7968 mean_circ: 0.947372
Ran for 241550 frames Cell Events: 7983 mean_circ: 0.947369
Ran for 242050 frames Cell Events: 7998 mean_circ: 0.947348
Ran for 242550 frames Cell Events: 8018 mean_circ: 0.947356
Ran for 243050 frames Cell Events: 8045 mean_circ: 0.947332
Ran for 243550 frames Cell Events: 8064 mean_circ: 0.947298
Ran for 244550 frames Cell Events: 8104 mean_circ: 0.947329
Ran for 245550 frames Cell Events: 8149 mean_circ: 0.947247



End this measurment..



Stopping time: 2.35395e+009
****************************************
Measurement finished

Mean Circularitiy: 0.947236
Mean Area: 595.517
Mean FL-1 intensity: 3254.98
Mean FL-2 intensity: 4043.6
Mean FL-3 intensity: 0

frames processed: 245578
Total Time: 123131ms
Number of Runs (new bg img) 0
Frames collected: 246043
Skipped frames: 11388
Rescued Frames: 852281
Samples DAQ: 61510796
Samples written: 123021540
Samples per Channel: 61510770
FPS: 1998.21

Samples per second: 499554
Expected samples/s: 500000

Events: 8169
Events per second: 66.3437
jumped back (PMT): 4456
#Frames read, frequency
# 0 frames :363843
# 1 frames :110326
# 2 frames :4579
# 3 frames :1852
# 4 frames :1184
# 5 frames :728
# 6 frames :973
# 7 frames :1504
# 8 frames :1207
# 9 frames :792
# 10 frames :710
# 11 frames :645
# 12 frames :562
# 13 frames :472
# 14 frames :383
# 15 frames :313
# 16 frames :293
# 17 frames :277
# 18 frames :243
# 19 frames :204
# 20 frames :161
# 21 frames :108
# 22 frames :78
# 23 frames :72
# 24 frames :65
# 25 frames :55
# 26 frames :52
# 27 frames :61
# 28 frames :36
# 29 frames :25
# 30 frames :37
# 31 frames :24
# 32 frames :20
# 33 frames :20
# 34 frames :11
# 35 frames :11
# 36 frames :14
# 37 frames :10
# 38 frames :15
# 39 frames :7
# 40 frames :7
# 41 frames :7
# 42 frames :4
# 43 frames :3
# 44 frames :5
# 45 frames :5
# 46 frames :2
# 47 frames :3
# 48 frames :6
# 49 frames :4
# 50 frames :1
# 51 frames :2
# 56 frames :1
# 58 frames :2
# 61 frames :2
# 69 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.04
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 6
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 24.8385
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 6
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 300
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0