Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-10_18.18.34 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "8d527781e9e4afca0aa911b530b9bb95",
"name": "M1_20170616_1631_dsRNA_o7_col.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "16:18:55"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8096 (max 8098) in '8096'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 24.7327
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 2.20827e+008
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 550 frames Cell Events: 31 mean_circ: 0.915205
Ran for 1550 frames Cell Events: 82 mean_circ: 0.93861
Ran for 3050 frames Cell Events: 154 mean_circ: 0.942292
Ran for 3550 frames Cell Events: 177 mean_circ: 0.940635
Ran for 4050 frames Cell Events: 207 mean_circ: 0.940635
Ran for 5050 frames Cell Events: 257 mean_circ: 0.942354
Ran for 5550 frames Cell Events: 275 mean_circ: 0.942585
Ran for 6050 frames Cell Events: 293 mean_circ: 0.942516
Ran for 6550 frames Cell Events: 320 mean_circ: 0.943333
Ran for 7550 frames Cell Events: 373 mean_circ: 0.944561
Ran for 8050 frames Cell Events: 406 mean_circ: 0.944656
Ran for 9050 frames Cell Events: 447 mean_circ: 0.946044
Ran for 9550 frames Cell Events: 463 mean_circ: 0.94631
Ran for 10050 frames Cell Events: 496 mean_circ: 0.947251
Ran for 10550 frames Cell Events: 514 mean_circ: 0.947831
Ran for 11050 frames Cell Events: 545 mean_circ: 0.947557
Ran for 11550 frames Cell Events: 577 mean_circ: 0.947938
Ran for 12050 frames Cell Events: 615 mean_circ: 0.947167
Ran for 14550 frames Cell Events: 759 mean_circ: 0.948051
Ran for 15550 frames Cell Events: 817 mean_circ: 0.948176
Ran for 16550 frames Cell Events: 868 mean_circ: 0.948253
Ran for 17050 frames Cell Events: 885 mean_circ: 0.948476
Ran for 17550 frames Cell Events: 909 mean_circ: 0.948682
Ran for 18050 frames Cell Events: 935 mean_circ: 0.94838
Ran for 19050 frames Cell Events: 1001 mean_circ: 0.948619
Ran for 20050 frames Cell Events: 1049 mean_circ: 0.948189
Ran for 20550 frames Cell Events: 1074 mean_circ: 0.948198
Ran for 21050 frames Cell Events: 1102 mean_circ: 0.948249
Ran for 24050 frames Cell Events: 1276 mean_circ: 0.948668
Ran for 24550 frames Cell Events: 1295 mean_circ: 0.948446
Ran for 25050 frames Cell Events: 1315 mean_circ: 0.948528
Ran for 27050 frames Cell Events: 1446 mean_circ: 0.94866
Ran for 27550 frames Cell Events: 1463 mean_circ: 0.948755
Ran for 28550 frames Cell Events: 1506 mean_circ: 0.948906
Ran for 29050 frames Cell Events: 1525 mean_circ: 0.948895
Ran for 31050 frames Cell Events: 1625 mean_circ: 0.948805
Ran for 32050 frames Cell Events: 1667 mean_circ: 0.948923
Ran for 34050 frames Cell Events: 1765 mean_circ: 0.949245
Ran for 34550 frames Cell Events: 1776 mean_circ: 0.949412
Ran for 35550 frames Cell Events: 1820 mean_circ: 0.949428
Ran for 36050 frames Cell Events: 1846 mean_circ: 0.949611
Ran for 36550 frames Cell Events: 1875 mean_circ: 0.949643
Ran for 37050 frames Cell Events: 1893 mean_circ: 0.94969
Ran for 38050 frames Cell Events: 1936 mean_circ: 0.949575
Ran for 38550 frames Cell Events: 1960 mean_circ: 0.949624
Ran for 39050 frames Cell Events: 1992 mean_circ: 0.94958
Ran for 39550 frames Cell Events: 2024 mean_circ: 0.949594
Ran for 40550 frames Cell Events: 2089 mean_circ: 0.949649
Ran for 41550 frames Cell Events: 2144 mean_circ: 0.949695
Ran for 44050 frames Cell Events: 2297 mean_circ: 0.949701
Ran for 44550 frames Cell Events: 2327 mean_circ: 0.949786
Ran for 45050 frames Cell Events: 2350 mean_circ: 0.949815
Ran for 45550 frames Cell Events: 2374 mean_circ: 0.949792
Ran for 46550 frames Cell Events: 2439 mean_circ: 0.949992
Ran for 47050 frames Cell Events: 2477 mean_circ: 0.949992
Ran for 49550 frames Cell Events: 2615 mean_circ: 0.950102
Ran for 50550 frames Cell Events: 2673 mean_circ: 0.950245
Ran for 52550 frames Cell Events: 2795 mean_circ: 0.950428
Ran for 53050 frames Cell Events: 2827 mean_circ: 0.950413
Ran for 53550 frames Cell Events: 2852 mean_circ: 0.950259
Ran for 55550 frames Cell Events: 2957 mean_circ: 0.950345
Ran for 58050 frames Cell Events: 3097 mean_circ: 0.950162
Ran for 58550 frames Cell Events: 3122 mean_circ: 0.950182
Ran for 60050 frames Cell Events: 3194 mean_circ: 0.950186
Ran for 61550 frames Cell Events: 3267 mean_circ: 0.950192
Ran for 63050 frames Cell Events: 3367 mean_circ: 0.950058
Ran for 64050 frames Cell Events: 3418 mean_circ: 0.949909
Ran for 65050 frames Cell Events: 3462 mean_circ: 0.949847
Ran for 65550 frames Cell Events: 3477 mean_circ: 0.949877
Ran for 67550 frames Cell Events: 3595 mean_circ: 0.949796
Ran for 68550 frames Cell Events: 3641 mean_circ: 0.949864
Ran for 70050 frames Cell Events: 3718 mean_circ: 0.949778
Ran for 71050 frames Cell Events: 3769 mean_circ: 0.949734
Ran for 71550 frames Cell Events: 3798 mean_circ: 0.94975
Ran for 72050 frames Cell Events: 3826 mean_circ: 0.949609
Ran for 74050 frames Cell Events: 3932 mean_circ: 0.949668
Ran for 74550 frames Cell Events: 3964 mean_circ: 0.949672
Ran for 75050 frames Cell Events: 3986 mean_circ: 0.94967
Ran for 76050 frames Cell Events: 4031 mean_circ: 0.94966
Ran for 76550 frames Cell Events: 4059 mean_circ: 0.949716
Ran for 77550 frames Cell Events: 4110 mean_circ: 0.949735
Ran for 78050 frames Cell Events: 4127 mean_circ: 0.949681
Ran for 79050 frames Cell Events: 4164 mean_circ: 0.949668
Ran for 79550 frames Cell Events: 4189 mean_circ: 0.949669
Ran for 81550 frames Cell Events: 4287 mean_circ: 0.949653
Ran for 82050 frames Cell Events: 4307 mean_circ: 0.949593
Ran for 82550 frames Cell Events: 4326 mean_circ: 0.949629
Ran for 83050 frames Cell Events: 4342 mean_circ: 0.949643
Ran for 83550 frames Cell Events: 4369 mean_circ: 0.949578
Ran for 84050 frames Cell Events: 4402 mean_circ: 0.949534
Ran for 86050 frames Cell Events: 4530 mean_circ: 0.949571
Ran for 86550 frames Cell Events: 4563 mean_circ: 0.949595
Ran for 87050 frames Cell Events: 4586 mean_circ: 0.949555
Ran for 87550 frames Cell Events: 4618 mean_circ: 0.949495
Ran for 88550 frames Cell Events: 4656 mean_circ: 0.949524
Ran for 89050 frames Cell Events: 4671 mean_circ: 0.949498
Ran for 90550 frames Cell Events: 4740 mean_circ: 0.949452
Ran for 91550 frames Cell Events: 4804 mean_circ: 0.949405
Ran for 92050 frames Cell Events: 4820 mean_circ: 0.949394
Ran for 93550 frames Cell Events: 4907 mean_circ: 0.949415
Ran for 94550 frames Cell Events: 4960 mean_circ: 0.949397
Ran for 100050 frames Cell Events: 5241 mean_circ: 0.94964
Ran for 100550 frames Cell Events: 5263 mean_circ: 0.949679
Ran for 101050 frames Cell Events: 5308 mean_circ: 0.949686
Ran for 101550 frames Cell Events: 5329 mean_circ: 0.949637
Ran for 102050 frames Cell Events: 5362 mean_circ: 0.949647
Ran for 102550 frames Cell Events: 5391 mean_circ: 0.949625
Ran for 103050 frames Cell Events: 5412 mean_circ: 0.949625
Ran for 103550 frames Cell Events: 5443 mean_circ: 0.949589
Ran for 107050 frames Cell Events: 5658 mean_circ: 0.949744
Ran for 108550 frames Cell Events: 5747 mean_circ: 0.94974
Ran for 109050 frames Cell Events: 5776 mean_circ: 0.94973
Ran for 109550 frames Cell Events: 5813 mean_circ: 0.949682
Ran for 110050 frames Cell Events: 5834 mean_circ: 0.94969
Ran for 110550 frames Cell Events: 5859 mean_circ: 0.949625
Ran for 112050 frames Cell Events: 5928 mean_circ: 0.949548
Ran for 114550 frames Cell Events: 6080 mean_circ: 0.949622
Ran for 115050 frames Cell Events: 6105 mean_circ: 0.949575
Ran for 116550 frames Cell Events: 6184 mean_circ: 0.949454
Ran for 117050 frames Cell Events: 6210 mean_circ: 0.949483
Ran for 117550 frames Cell Events: 6231 mean_circ: 0.949477
Ran for 118050 frames Cell Events: 6253 mean_circ: 0.949479
Ran for 118550 frames Cell Events: 6280 mean_circ: 0.949465
Ran for 120550 frames Cell Events: 6395 mean_circ: 0.949478
Ran for 121050 frames Cell Events: 6420 mean_circ: 0.949473
Ran for 121550 frames Cell Events: 6444 mean_circ: 0.949467
Ran for 123050 frames Cell Events: 6529 mean_circ: 0.949473
Ran for 124550 frames Cell Events: 6612 mean_circ: 0.949467
Ran for 126050 frames Cell Events: 6678 mean_circ: 0.949465
Ran for 127050 frames Cell Events: 6729 mean_circ: 0.949459
Ran for 128550 frames Cell Events: 6813 mean_circ: 0.949501
Ran for 129050 frames Cell Events: 6837 mean_circ: 0.949518
Ran for 130050 frames Cell Events: 6887 mean_circ: 0.949474
Ran for 130550 frames Cell Events: 6914 mean_circ: 0.949383
Ran for 131550 frames Cell Events: 6975 mean_circ: 0.949339
Ran for 132050 frames Cell Events: 7003 mean_circ: 0.949327
Ran for 135050 frames Cell Events: 7173 mean_circ: 0.949384
Ran for 137050 frames Cell Events: 7286 mean_circ: 0.949413
Ran for 139050 frames Cell Events: 7414 mean_circ: 0.949381
Ran for 140550 frames Cell Events: 7515 mean_circ: 0.949382
Ran for 141550 frames Cell Events: 7587 mean_circ: 0.949303
Ran for 142550 frames Cell Events: 7647 mean_circ: 0.949274
Ran for 143050 frames Cell Events: 7677 mean_circ: 0.949279
Ran for 143550 frames Cell Events: 7708 mean_circ: 0.949234
Ran for 146050 frames Cell Events: 7837 mean_circ: 0.949072
Ran for 147050 frames Cell Events: 7898 mean_circ: 0.949031
Ran for 149550 frames Cell Events: 8046 mean_circ: 0.94908
Ran for 150050 frames Cell Events: 8064 mean_circ: 0.949069



End this measurment..



Stopping time: 2.20902e+008
****************************************
Measurement finished

Mean Circularitiy: 0.949078
Mean Area: 603.186
Mean FL-1 intensity: 3025.4
Mean FL-2 intensity: 3485.26
Mean FL-3 intensity: 0

frames processed: 149142
Total Time: 75339ms
Number of Runs (new bg img) 0
Frames collected: 150513
Skipped frames: 38138
Rescued Frames: 3188977
Samples DAQ: 37629770
Samples written: 75256410
Samples per Channel: 37628205
FPS: 1997.81

Samples per second: 499452
Expected samples/s: 500000

Events: 8098
Events per second: 107.487
jumped back (PMT): 3455
#Frames read, frequency
# 0 frames :237974
# 1 frames :50308
# 2 frames :2968
# 3 frames :1178
# 4 frames :706
# 5 frames :396
# 6 frames :1205
# 7 frames :1149
# 8 frames :828
# 9 frames :618
# 10 frames :765
# 11 frames :483
# 12 frames :343
# 13 frames :218
# 14 frames :218
# 15 frames :189
# 16 frames :182
# 17 frames :139
# 18 frames :139
# 19 frames :101
# 20 frames :87
# 21 frames :80
# 22 frames :80
# 23 frames :51
# 24 frames :49
# 25 frames :46
# 26 frames :39
# 27 frames :41
# 28 frames :29
# 29 frames :34
# 30 frames :22
# 31 frames :28
# 32 frames :21
# 33 frames :26
# 34 frames :19
# 35 frames :13
# 36 frames :16
# 37 frames :18
# 38 frames :9
# 39 frames :10
# 40 frames :7
# 41 frames :11
# 42 frames :5
# 43 frames :10
# 44 frames :6
# 45 frames :5
# 46 frames :5
# 47 frames :1
# 48 frames :4
# 49 frames :5
# 50 frames :6
# 51 frames :2
# 52 frames :1
# 53 frames :3
# 55 frames :2
# 56 frames :1
# 57 frames :1
# 58 frames :1
# 59 frames :1
# 60 frames :2
# 62 frames :1
# 68 frames :1
# 72 frames :1
# 73 frames :1
# 77 frames :1
# 79 frames :1
# 81 frames :1
# 86 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.04
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 6
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 24.7327
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 6
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 300
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0