Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-10_18.33.03 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "9dd62414a9c3ca44c94c001d69292799",
"name": "M1_20170616_2000_dsRNA_P6-col.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "16:33:23"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8048 (max 8049) in '8048'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 24.5489
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 2.3335e+008
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 550 frames Cell Events: 22 mean_circ: 0.970797
Ran for 1550 frames Cell Events: 71 mean_circ: 0.958503
Ran for 3050 frames Cell Events: 145 mean_circ: 0.95414
Ran for 4550 frames Cell Events: 231 mean_circ: 0.953619
Ran for 6050 frames Cell Events: 303 mean_circ: 0.952748
Ran for 7550 frames Cell Events: 388 mean_circ: 0.953622
Ran for 8050 frames Cell Events: 411 mean_circ: 0.953387
Ran for 8550 frames Cell Events: 438 mean_circ: 0.953221
Ran for 9050 frames Cell Events: 468 mean_circ: 0.953052
Ran for 10550 frames Cell Events: 535 mean_circ: 0.953476
Ran for 11050 frames Cell Events: 556 mean_circ: 0.953626
Ran for 13550 frames Cell Events: 655 mean_circ: 0.953542
Ran for 14050 frames Cell Events: 671 mean_circ: 0.953155
Ran for 14550 frames Cell Events: 685 mean_circ: 0.953439
Ran for 15550 frames Cell Events: 719 mean_circ: 0.953535
Ran for 17550 frames Cell Events: 808 mean_circ: 0.953354
Ran for 18050 frames Cell Events: 828 mean_circ: 0.953355
Ran for 18550 frames Cell Events: 843 mean_circ: 0.953448
Ran for 19050 frames Cell Events: 862 mean_circ: 0.953448
Ran for 19550 frames Cell Events: 884 mean_circ: 0.953518
Ran for 20050 frames Cell Events: 911 mean_circ: 0.95327
Ran for 22550 frames Cell Events: 1002 mean_circ: 0.953435
Ran for 23050 frames Cell Events: 1019 mean_circ: 0.953621
Ran for 24050 frames Cell Events: 1050 mean_circ: 0.953185
Ran for 26550 frames Cell Events: 1144 mean_circ: 0.952438
Ran for 27050 frames Cell Events: 1158 mean_circ: 0.952524
Ran for 27550 frames Cell Events: 1168 mean_circ: 0.952633
Ran for 28050 frames Cell Events: 1193 mean_circ: 0.952688
Ran for 28550 frames Cell Events: 1213 mean_circ: 0.952604
Ran for 29050 frames Cell Events: 1228 mean_circ: 0.952616
Ran for 30050 frames Cell Events: 1261 mean_circ: 0.952788
Ran for 30550 frames Cell Events: 1274 mean_circ: 0.952771
Ran for 31050 frames Cell Events: 1297 mean_circ: 0.95278
Ran for 31550 frames Cell Events: 1314 mean_circ: 0.952958
Ran for 32550 frames Cell Events: 1366 mean_circ: 0.952975
Ran for 34550 frames Cell Events: 1468 mean_circ: 0.952772
Ran for 35050 frames Cell Events: 1505 mean_circ: 0.952259
Ran for 35550 frames Cell Events: 1536 mean_circ: 0.952083
Ran for 36550 frames Cell Events: 1572 mean_circ: 0.952246
Ran for 37050 frames Cell Events: 1588 mean_circ: 0.952308
Ran for 37550 frames Cell Events: 1613 mean_circ: 0.952351
Ran for 38050 frames Cell Events: 1633 mean_circ: 0.95218
Ran for 38550 frames Cell Events: 1652 mean_circ: 0.952155
Ran for 39050 frames Cell Events: 1686 mean_circ: 0.952216
Ran for 40050 frames Cell Events: 1733 mean_circ: 0.952408
Ran for 40550 frames Cell Events: 1752 mean_circ: 0.952264
Ran for 41550 frames Cell Events: 1818 mean_circ: 0.952371
Ran for 42050 frames Cell Events: 1851 mean_circ: 0.952405
Ran for 42550 frames Cell Events: 1872 mean_circ: 0.952286
Ran for 43050 frames Cell Events: 1900 mean_circ: 0.952222
Ran for 44550 frames Cell Events: 1995 mean_circ: 0.952212
Ran for 46050 frames Cell Events: 2069 mean_circ: 0.952027
Ran for 46550 frames Cell Events: 2092 mean_circ: 0.952155
Ran for 47050 frames Cell Events: 2113 mean_circ: 0.952114
Ran for 49050 frames Cell Events: 2215 mean_circ: 0.952188
Ran for 50050 frames Cell Events: 2261 mean_circ: 0.952274
Ran for 50550 frames Cell Events: 2277 mean_circ: 0.952324
Ran for 51050 frames Cell Events: 2302 mean_circ: 0.952324
Ran for 51550 frames Cell Events: 2326 mean_circ: 0.952368
Ran for 52050 frames Cell Events: 2340 mean_circ: 0.952358
Ran for 53550 frames Cell Events: 2405 mean_circ: 0.952327
Ran for 54050 frames Cell Events: 2435 mean_circ: 0.952192
Ran for 57050 frames Cell Events: 2612 mean_circ: 0.952301
Ran for 57550 frames Cell Events: 2627 mean_circ: 0.952314
Ran for 58050 frames Cell Events: 2649 mean_circ: 0.952365
Ran for 58550 frames Cell Events: 2664 mean_circ: 0.952342
Ran for 59050 frames Cell Events: 2696 mean_circ: 0.952367
Ran for 59550 frames Cell Events: 2718 mean_circ: 0.952324
Ran for 61050 frames Cell Events: 2802 mean_circ: 0.95233
Ran for 61550 frames Cell Events: 2828 mean_circ: 0.952364
Ran for 63050 frames Cell Events: 2918 mean_circ: 0.952348
Ran for 64050 frames Cell Events: 2996 mean_circ: 0.952396
Ran for 67050 frames Cell Events: 3180 mean_circ: 0.952383
Ran for 68550 frames Cell Events: 3278 mean_circ: 0.952445
Ran for 69050 frames Cell Events: 3306 mean_circ: 0.952417
Ran for 69550 frames Cell Events: 3339 mean_circ: 0.95238
Ran for 70050 frames Cell Events: 3380 mean_circ: 0.95238
Ran for 70550 frames Cell Events: 3413 mean_circ: 0.952292
Ran for 71550 frames Cell Events: 3454 mean_circ: 0.952289
Ran for 73550 frames Cell Events: 3587 mean_circ: 0.95221
Ran for 74550 frames Cell Events: 3623 mean_circ: 0.952204
Ran for 75050 frames Cell Events: 3653 mean_circ: 0.952229
Ran for 75550 frames Cell Events: 3681 mean_circ: 0.952205
Ran for 76550 frames Cell Events: 3739 mean_circ: 0.952337
Ran for 77050 frames Cell Events: 3755 mean_circ: 0.952295
Ran for 78550 frames Cell Events: 3856 mean_circ: 0.952338
Ran for 79050 frames Cell Events: 3880 mean_circ: 0.952383
Ran for 79550 frames Cell Events: 3906 mean_circ: 0.95237
Ran for 80050 frames Cell Events: 3920 mean_circ: 0.95238
Ran for 83050 frames Cell Events: 4007 mean_circ: 0.952476
Ran for 84550 frames Cell Events: 4080 mean_circ: 0.952542
Ran for 85550 frames Cell Events: 4128 mean_circ: 0.952515
Ran for 86050 frames Cell Events: 4155 mean_circ: 0.952519
Ran for 88050 frames Cell Events: 4278 mean_circ: 0.952686
Ran for 88550 frames Cell Events: 4305 mean_circ: 0.952657
Ran for 89550 frames Cell Events: 4365 mean_circ: 0.95272
Ran for 90050 frames Cell Events: 4395 mean_circ: 0.952779
Ran for 90550 frames Cell Events: 4434 mean_circ: 0.952779
Ran for 91550 frames Cell Events: 4483 mean_circ: 0.952725
Ran for 92050 frames Cell Events: 4516 mean_circ: 0.952724
Ran for 92550 frames Cell Events: 4536 mean_circ: 0.952749
Ran for 94050 frames Cell Events: 4627 mean_circ: 0.952731
Ran for 94550 frames Cell Events: 4670 mean_circ: 0.952686
Ran for 96050 frames Cell Events: 4744 mean_circ: 0.952649
Ran for 96550 frames Cell Events: 4780 mean_circ: 0.952544
Ran for 98050 frames Cell Events: 4881 mean_circ: 0.952623
Ran for 100550 frames Cell Events: 5028 mean_circ: 0.95256
Ran for 103050 frames Cell Events: 5209 mean_circ: 0.952435
Ran for 104050 frames Cell Events: 5261 mean_circ: 0.952383
Ran for 105550 frames Cell Events: 5353 mean_circ: 0.952395
Ran for 106550 frames Cell Events: 5427 mean_circ: 0.952367
Ran for 107550 frames Cell Events: 5503 mean_circ: 0.952302
Ran for 108550 frames Cell Events: 5525 mean_circ: 0.952322
Ran for 110050 frames Cell Events: 5637 mean_circ: 0.952346
Ran for 110550 frames Cell Events: 5666 mean_circ: 0.952342
Ran for 116050 frames Cell Events: 6010 mean_circ: 0.951975
Ran for 117050 frames Cell Events: 6071 mean_circ: 0.951896
Ran for 120050 frames Cell Events: 6258 mean_circ: 0.951733
Ran for 121050 frames Cell Events: 6303 mean_circ: 0.951765
Ran for 122050 frames Cell Events: 6359 mean_circ: 0.951797
Ran for 122550 frames Cell Events: 6387 mean_circ: 0.951812
Ran for 125050 frames Cell Events: 6532 mean_circ: 0.951747
Ran for 125550 frames Cell Events: 6555 mean_circ: 0.951704
Ran for 127050 frames Cell Events: 6625 mean_circ: 0.951721
Ran for 127550 frames Cell Events: 6653 mean_circ: 0.95172
Ran for 128050 frames Cell Events: 6688 mean_circ: 0.951713
Ran for 129050 frames Cell Events: 6748 mean_circ: 0.951694
Ran for 130050 frames Cell Events: 6771 mean_circ: 0.951705
Ran for 131050 frames Cell Events: 6835 mean_circ: 0.951688
Ran for 131550 frames Cell Events: 6857 mean_circ: 0.951711
Ran for 132050 frames Cell Events: 6877 mean_circ: 0.951751
Ran for 134550 frames Cell Events: 6947 mean_circ: 0.951785
Ran for 135550 frames Cell Events: 7005 mean_circ: 0.951794
Ran for 136550 frames Cell Events: 7053 mean_circ: 0.951769
Ran for 138050 frames Cell Events: 7132 mean_circ: 0.951774
Ran for 138550 frames Cell Events: 7155 mean_circ: 0.951802
Ran for 139050 frames Cell Events: 7174 mean_circ: 0.951819
Ran for 139550 frames Cell Events: 7205 mean_circ: 0.951836
Ran for 140050 frames Cell Events: 7216 mean_circ: 0.951832
Ran for 140550 frames Cell Events: 7241 mean_circ: 0.95184
Ran for 141050 frames Cell Events: 7260 mean_circ: 0.951862
Ran for 141550 frames Cell Events: 7294 mean_circ: 0.951878
Ran for 142050 frames Cell Events: 7311 mean_circ: 0.951843
Ran for 143050 frames Cell Events: 7365 mean_circ: 0.951896
Ran for 143550 frames Cell Events: 7388 mean_circ: 0.951862
Ran for 144550 frames Cell Events: 7436 mean_circ: 0.951863
Ran for 145550 frames Cell Events: 7508 mean_circ: 0.951774
Ran for 146050 frames Cell Events: 7526 mean_circ: 0.951812
Ran for 149550 frames Cell Events: 7718 mean_circ: 0.951773
Ran for 150050 frames Cell Events: 7743 mean_circ: 0.951746
Ran for 151050 frames Cell Events: 7799 mean_circ: 0.951778
Ran for 152550 frames Cell Events: 7874 mean_circ: 0.951771
Ran for 153050 frames Cell Events: 7894 mean_circ: 0.951748
Ran for 154550 frames Cell Events: 8009 mean_circ: 0.951665
Ran for 155050 frames Cell Events: 8036 mean_circ: 0.951642



End this measurment..



Stopping time: 2.33428e+008
****************************************
Measurement finished

Mean Circularitiy: 0.951659
Mean Area: 595.844
Mean FL-1 intensity: 3138.69
Mean FL-2 intensity: 3505.98
Mean FL-3 intensity: 0

frames processed: 151010
Total Time: 77764.5ms
Number of Runs (new bg img) 0
Frames collected: 155415
Skipped frames: 78633
Rescued Frames: 6316445
Samples DAQ: 38856004
Samples written: 77707480
Samples per Channel: 38853740
FPS: 1998.53

Samples per second: 499633
Expected samples/s: 500000

Events: 8049
Events per second: 103.505
jumped back (PMT): 3752
#Frames read, frequency
# 0 frames :241883
# 1 frames :56174
# 2 frames :3205
# 3 frames :1132
# 4 frames :652
# 5 frames :345
# 6 frames :1542
# 7 frames :1277
# 8 frames :847
# 9 frames :548
# 10 frames :605
# 11 frames :352
# 12 frames :301
# 13 frames :221
# 14 frames :240
# 15 frames :216
# 16 frames :167
# 17 frames :138
# 18 frames :112
# 19 frames :92
# 20 frames :93
# 21 frames :75
# 22 frames :63
# 23 frames :57
# 24 frames :53
# 25 frames :52
# 26 frames :34
# 27 frames :30
# 28 frames :29
# 29 frames :29
# 30 frames :28
# 31 frames :26
# 32 frames :13
# 33 frames :16
# 34 frames :6
# 35 frames :14
# 36 frames :12
# 37 frames :13
# 38 frames :5
# 39 frames :9
# 40 frames :9
# 41 frames :14
# 42 frames :3
# 43 frames :3
# 44 frames :3
# 45 frames :3
# 46 frames :2
# 47 frames :7
# 48 frames :2
# 49 frames :3
# 50 frames :2
# 51 frames :1
# 52 frames :1
# 53 frames :3
# 54 frames :1
# 55 frames :4
# 56 frames :3
# 58 frames :3
# 63 frames :1
# 66 frames :1
# 76 frames :1
# 84 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.04
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 6
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 24.5489
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 6
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 300
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0