Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-10_18.48.28 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "6e486ba8b8b24a919f50c06020e2fbb2",
"name": "M1_20170618_1555_dsRNA_Q3_col.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "16:48:49"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8046 (max 8047) in '8046'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 25.0811
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 3.91498e+008
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 2050 frames Cell Events: 68 mean_circ: 0.955179
Ran for 2550 frames Cell Events: 90 mean_circ: 0.95479
Ran for 3050 frames Cell Events: 103 mean_circ: 0.954982
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Ran for 6550 frames Cell Events: 233 mean_circ: 0.954242
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Ran for 7550 frames Cell Events: 254 mean_circ: 0.95412
Ran for 8050 frames Cell Events: 270 mean_circ: 0.953697
Ran for 8550 frames Cell Events: 293 mean_circ: 0.951941
Ran for 9050 frames Cell Events: 319 mean_circ: 0.952867
Ran for 10050 frames Cell Events: 357 mean_circ: 0.952543
Ran for 10550 frames Cell Events: 366 mean_circ: 0.952332
Ran for 11050 frames Cell Events: 382 mean_circ: 0.952371
Ran for 11550 frames Cell Events: 407 mean_circ: 0.9526
Ran for 12050 frames Cell Events: 428 mean_circ: 0.951824
Ran for 12550 frames Cell Events: 445 mean_circ: 0.952257
Ran for 13050 frames Cell Events: 465 mean_circ: 0.952701
Ran for 14050 frames Cell Events: 489 mean_circ: 0.952859
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Ran for 15050 frames Cell Events: 522 mean_circ: 0.953119
Ran for 15550 frames Cell Events: 538 mean_circ: 0.953106
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Ran for 34050 frames Cell Events: 1223 mean_circ: 0.954206
Ran for 35050 frames Cell Events: 1257 mean_circ: 0.95431
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Ran for 36050 frames Cell Events: 1290 mean_circ: 0.954389
Ran for 36550 frames Cell Events: 1306 mean_circ: 0.954399
Ran for 38050 frames Cell Events: 1358 mean_circ: 0.954023
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Ran for 63550 frames Cell Events: 2275 mean_circ: 0.953482
Ran for 64050 frames Cell Events: 2301 mean_circ: 0.953405
Ran for 64550 frames Cell Events: 2323 mean_circ: 0.953411
Ran for 65550 frames Cell Events: 2363 mean_circ: 0.953545
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Ran for 67050 frames Cell Events: 2427 mean_circ: 0.953572
Ran for 67550 frames Cell Events: 2442 mean_circ: 0.953531
Ran for 68550 frames Cell Events: 2473 mean_circ: 0.953571
Ran for 69050 frames Cell Events: 2484 mean_circ: 0.953634
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Ran for 70050 frames Cell Events: 2513 mean_circ: 0.953608
Ran for 71050 frames Cell Events: 2546 mean_circ: 0.953655
Ran for 71550 frames Cell Events: 2566 mean_circ: 0.953679
Ran for 72050 frames Cell Events: 2576 mean_circ: 0.953717
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Ran for 73050 frames Cell Events: 2609 mean_circ: 0.953737
Ran for 73550 frames Cell Events: 2628 mean_circ: 0.953705
Ran for 74050 frames Cell Events: 2642 mean_circ: 0.953689
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Ran for 76550 frames Cell Events: 2735 mean_circ: 0.95375
Ran for 77550 frames Cell Events: 2769 mean_circ: 0.953757
Ran for 78050 frames Cell Events: 2781 mean_circ: 0.953773
Ran for 78550 frames Cell Events: 2799 mean_circ: 0.953782
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Ran for 80050 frames Cell Events: 2842 mean_circ: 0.953534
Ran for 80550 frames Cell Events: 2853 mean_circ: 0.953582
Ran for 81050 frames Cell Events: 2870 mean_circ: 0.953582
Ran for 82050 frames Cell Events: 2902 mean_circ: 0.953616
Ran for 82550 frames Cell Events: 2922 mean_circ: 0.953701
Ran for 83050 frames Cell Events: 2944 mean_circ: 0.953655
Ran for 84050 frames Cell Events: 2975 mean_circ: 0.953674
Ran for 84550 frames Cell Events: 3001 mean_circ: 0.953757
Ran for 85050 frames Cell Events: 3022 mean_circ: 0.953782
Ran for 85550 frames Cell Events: 3037 mean_circ: 0.953679
Ran for 86550 frames Cell Events: 3077 mean_circ: 0.953695
Ran for 87050 frames Cell Events: 3087 mean_circ: 0.953725
Ran for 88050 frames Cell Events: 3125 mean_circ: 0.953722
Ran for 88550 frames Cell Events: 3146 mean_circ: 0.953749
Ran for 89050 frames Cell Events: 3164 mean_circ: 0.953663
Ran for 89550 frames Cell Events: 3182 mean_circ: 0.953679
Ran for 90050 frames Cell Events: 3199 mean_circ: 0.953673
Ran for 91050 frames Cell Events: 3237 mean_circ: 0.953729
Ran for 91550 frames Cell Events: 3248 mean_circ: 0.953768
Ran for 92050 frames Cell Events: 3261 mean_circ: 0.953756
Ran for 93050 frames Cell Events: 3305 mean_circ: 0.953851
Ran for 93550 frames Cell Events: 3316 mean_circ: 0.953864
Ran for 94050 frames Cell Events: 3332 mean_circ: 0.953901
Ran for 95050 frames Cell Events: 3366 mean_circ: 0.953918
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Ran for 96050 frames Cell Events: 3397 mean_circ: 0.953905
Ran for 96550 frames Cell Events: 3427 mean_circ: 0.95395
Ran for 97050 frames Cell Events: 3444 mean_circ: 0.953938
Ran for 98050 frames Cell Events: 3481 mean_circ: 0.953877
Ran for 99550 frames Cell Events: 3529 mean_circ: 0.953913
Ran for 100550 frames Cell Events: 3561 mean_circ: 0.953929
Ran for 101550 frames Cell Events: 3602 mean_circ: 0.953854
Ran for 102050 frames Cell Events: 3632 mean_circ: 0.953873
Ran for 103050 frames Cell Events: 3671 mean_circ: 0.953932
Ran for 103550 frames Cell Events: 3694 mean_circ: 0.953938
Ran for 105550 frames Cell Events: 3809 mean_circ: 0.95398
Ran for 106550 frames Cell Events: 3864 mean_circ: 0.953824
Ran for 107550 frames Cell Events: 3916 mean_circ: 0.953775
Ran for 108050 frames Cell Events: 3938 mean_circ: 0.953801
Ran for 108550 frames Cell Events: 3954 mean_circ: 0.953847
Ran for 110050 frames Cell Events: 4008 mean_circ: 0.953789
Ran for 111050 frames Cell Events: 4056 mean_circ: 0.953824
Ran for 111550 frames Cell Events: 4086 mean_circ: 0.95384
Ran for 112550 frames Cell Events: 4117 mean_circ: 0.953856
Ran for 113550 frames Cell Events: 4153 mean_circ: 0.953922
Ran for 114050 frames Cell Events: 4170 mean_circ: 0.953946
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Ran for 115050 frames Cell Events: 4204 mean_circ: 0.95391
Ran for 115550 frames Cell Events: 4226 mean_circ: 0.953968
Ran for 116050 frames Cell Events: 4248 mean_circ: 0.953986
Ran for 117550 frames Cell Events: 4310 mean_circ: 0.95389
Ran for 118550 frames Cell Events: 4344 mean_circ: 0.953869
Ran for 119050 frames Cell Events: 4373 mean_circ: 0.953867
Ran for 119550 frames Cell Events: 4398 mean_circ: 0.953746
Ran for 120050 frames Cell Events: 4417 mean_circ: 0.953762
Ran for 120550 frames Cell Events: 4436 mean_circ: 0.953761
Ran for 121050 frames Cell Events: 4454 mean_circ: 0.953764
Ran for 122550 frames Cell Events: 4520 mean_circ: 0.953809
Ran for 125050 frames Cell Events: 4629 mean_circ: 0.953929
Ran for 126050 frames Cell Events: 4665 mean_circ: 0.953914
Ran for 126550 frames Cell Events: 4683 mean_circ: 0.953889
Ran for 127050 frames Cell Events: 4695 mean_circ: 0.953869
Ran for 127550 frames Cell Events: 4717 mean_circ: 0.953932
Ran for 128050 frames Cell Events: 4734 mean_circ: 0.953956
Ran for 128550 frames Cell Events: 4753 mean_circ: 0.953988
Ran for 129050 frames Cell Events: 4766 mean_circ: 0.953988
Ran for 130050 frames Cell Events: 4800 mean_circ: 0.953977
Ran for 130550 frames Cell Events: 4820 mean_circ: 0.953984
Ran for 131050 frames Cell Events: 4836 mean_circ: 0.954007
Ran for 131550 frames Cell Events: 4851 mean_circ: 0.954028
Ran for 132050 frames Cell Events: 4876 mean_circ: 0.953941
Ran for 132550 frames Cell Events: 4907 mean_circ: 0.953915
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Ran for 138050 frames Cell Events: 5107 mean_circ: 0.953752
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Ran for 141050 frames Cell Events: 5220 mean_circ: 0.953726
Ran for 141550 frames Cell Events: 5245 mean_circ: 0.953761
Ran for 142050 frames Cell Events: 5267 mean_circ: 0.953744
Ran for 143550 frames Cell Events: 5312 mean_circ: 0.953754
Ran for 144050 frames Cell Events: 5327 mean_circ: 0.953804
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Ran for 146550 frames Cell Events: 5428 mean_circ: 0.953746
Ran for 147050 frames Cell Events: 5450 mean_circ: 0.953775
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Ran for 152050 frames Cell Events: 5608 mean_circ: 0.953774
Ran for 152550 frames Cell Events: 5628 mean_circ: 0.953767
Ran for 153550 frames Cell Events: 5673 mean_circ: 0.953779
Ran for 154050 frames Cell Events: 5686 mean_circ: 0.953761
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Ran for 157550 frames Cell Events: 5822 mean_circ: 0.953746
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Ran for 162050 frames Cell Events: 5968 mean_circ: 0.953693
Ran for 162550 frames Cell Events: 5985 mean_circ: 0.953707
Ran for 163550 frames Cell Events: 6016 mean_circ: 0.953691
Ran for 164050 frames Cell Events: 6034 mean_circ: 0.953652
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Ran for 166050 frames Cell Events: 6128 mean_circ: 0.953644
Ran for 166550 frames Cell Events: 6143 mean_circ: 0.95359
Ran for 167550 frames Cell Events: 6177 mean_circ: 0.953573
Ran for 168050 frames Cell Events: 6191 mean_circ: 0.95356
Ran for 168550 frames Cell Events: 6212 mean_circ: 0.953603
Ran for 169550 frames Cell Events: 6252 mean_circ: 0.953586
Ran for 170050 frames Cell Events: 6274 mean_circ: 0.953573
Ran for 171550 frames Cell Events: 6334 mean_circ: 0.953475
Ran for 172050 frames Cell Events: 6349 mean_circ: 0.953494
Ran for 172550 frames Cell Events: 6369 mean_circ: 0.953505
Ran for 173550 frames Cell Events: 6408 mean_circ: 0.953508
Ran for 174050 frames Cell Events: 6425 mean_circ: 0.953503
Ran for 174550 frames Cell Events: 6444 mean_circ: 0.953512
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Ran for 176050 frames Cell Events: 6488 mean_circ: 0.953496
Ran for 176550 frames Cell Events: 6503 mean_circ: 0.953483
Ran for 177050 frames Cell Events: 6521 mean_circ: 0.953473
Ran for 177550 frames Cell Events: 6540 mean_circ: 0.953486
Ran for 178550 frames Cell Events: 6577 mean_circ: 0.953513
Ran for 179050 frames Cell Events: 6600 mean_circ: 0.953511
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Ran for 180050 frames Cell Events: 6623 mean_circ: 0.953478
Ran for 180550 frames Cell Events: 6646 mean_circ: 0.9535
Ran for 181050 frames Cell Events: 6667 mean_circ: 0.953491
Ran for 181550 frames Cell Events: 6682 mean_circ: 0.953479
Ran for 182050 frames Cell Events: 6685 mean_circ: 0.953486
Ran for 182550 frames Cell Events: 6696 mean_circ: 0.953519
Ran for 183550 frames Cell Events: 6733 mean_circ: 0.953497
Ran for 184050 frames Cell Events: 6756 mean_circ: 0.953496
Ran for 184550 frames Cell Events: 6774 mean_circ: 0.953504
Ran for 185050 frames Cell Events: 6788 mean_circ: 0.953531
Ran for 185550 frames Cell Events: 6804 mean_circ: 0.95352
Ran for 186050 frames Cell Events: 6819 mean_circ: 0.953552
Ran for 186550 frames Cell Events: 6834 mean_circ: 0.953556
Ran for 187050 frames Cell Events: 6862 mean_circ: 0.953537
Ran for 187550 frames Cell Events: 6876 mean_circ: 0.953535
Ran for 188050 frames Cell Events: 6894 mean_circ: 0.953532
Ran for 188550 frames Cell Events: 6909 mean_circ: 0.953537
Ran for 189050 frames Cell Events: 6925 mean_circ: 0.953527
Ran for 190050 frames Cell Events: 6963 mean_circ: 0.953582
Ran for 191050 frames Cell Events: 7001 mean_circ: 0.953612
Ran for 191550 frames Cell Events: 7026 mean_circ: 0.953614
Ran for 193050 frames Cell Events: 7077 mean_circ: 0.953655
Ran for 194050 frames Cell Events: 7117 mean_circ: 0.953669
Ran for 194550 frames Cell Events: 7130 mean_circ: 0.953632
Ran for 195550 frames Cell Events: 7162 mean_circ: 0.953632
Ran for 196050 frames Cell Events: 7183 mean_circ: 0.953621
Ran for 196550 frames Cell Events: 7196 mean_circ: 0.953632
Ran for 197050 frames Cell Events: 7219 mean_circ: 0.953647
Ran for 197550 frames Cell Events: 7247 mean_circ: 0.953681
Ran for 199050 frames Cell Events: 7318 mean_circ: 0.953719
Ran for 199550 frames Cell Events: 7336 mean_circ: 0.953682
Ran for 200050 frames Cell Events: 7351 mean_circ: 0.953685
Ran for 201050 frames Cell Events: 7391 mean_circ: 0.953721
Ran for 201550 frames Cell Events: 7403 mean_circ: 0.953693
Ran for 203550 frames Cell Events: 7463 mean_circ: 0.95364
Ran for 204050 frames Cell Events: 7477 mean_circ: 0.953623
Ran for 204550 frames Cell Events: 7492 mean_circ: 0.953591
Ran for 205050 frames Cell Events: 7511 mean_circ: 0.953584
Ran for 205550 frames Cell Events: 7529 mean_circ: 0.953582
Ran for 206050 frames Cell Events: 7554 mean_circ: 0.95356
Ran for 206550 frames Cell Events: 7571 mean_circ: 0.953546
Ran for 207050 frames Cell Events: 7582 mean_circ: 0.953548
Ran for 207550 frames Cell Events: 7589 mean_circ: 0.95355
Ran for 208050 frames Cell Events: 7608 mean_circ: 0.95356
Ran for 208550 frames Cell Events: 7619 mean_circ: 0.953561
Ran for 209050 frames Cell Events: 7638 mean_circ: 0.953578
Ran for 209550 frames Cell Events: 7656 mean_circ: 0.953597
Ran for 210050 frames Cell Events: 7683 mean_circ: 0.953594
Ran for 210550 frames Cell Events: 7699 mean_circ: 0.953621
Ran for 211050 frames Cell Events: 7715 mean_circ: 0.953634
Ran for 211550 frames Cell Events: 7738 mean_circ: 0.953643
Ran for 212550 frames Cell Events: 7766 mean_circ: 0.953653
Ran for 213550 frames Cell Events: 7818 mean_circ: 0.953627
Ran for 214050 frames Cell Events: 7834 mean_circ: 0.953643
Ran for 214550 frames Cell Events: 7851 mean_circ: 0.953669
Ran for 215050 frames Cell Events: 7867 mean_circ: 0.953693
Ran for 215550 frames Cell Events: 7894 mean_circ: 0.953709
Ran for 216550 frames Cell Events: 7948 mean_circ: 0.95369
Ran for 217050 frames Cell Events: 7966 mean_circ: 0.9537
Ran for 217550 frames Cell Events: 7988 mean_circ: 0.953697
Ran for 218550 frames Cell Events: 8028 mean_circ: 0.953711
Ran for 219050 frames Cell Events: 8047 mean_circ: 0.953711



End this measurment..



Stopping time: 3.91608e+008
****************************************
Measurement finished

Mean Circularitiy: 0.953711
Mean Area: 566.803
Mean FL-1 intensity: 2880.32
Mean FL-2 intensity: 3498.36
Mean FL-3 intensity: 0

frames processed: 218986
Total Time: 109649ms
Number of Runs (new bg img) 0
Frames collected: 219114
Skipped frames: 16718
Rescued Frames: 2482723
Samples DAQ: 54779288
Samples written: 109556850
Samples per Channel: 54778425
FPS: 1998.32

Samples per second: 499579
Expected samples/s: 500000

Events: 8047
Events per second: 73.3886
jumped back (PMT): 6114
#Frames read, frequency
# 0 frames :303797
# 1 frames :117660
# 2 frames :4914
# 3 frames :1782
# 4 frames :810
# 5 frames :318
# 6 frames :2231
# 7 frames :1678
# 8 frames :969
# 9 frames :546
# 10 frames :1415
# 11 frames :475
# 12 frames :382
# 13 frames :231
# 14 frames :222
# 15 frames :155
# 16 frames :162
# 17 frames :124
# 18 frames :71
# 19 frames :30
# 20 frames :38
# 21 frames :30
# 22 frames :25
# 23 frames :23
# 24 frames :18
# 25 frames :19
# 26 frames :15
# 27 frames :8
# 28 frames :2
# 29 frames :3
# 30 frames :2
# 31 frames :2
# 32 frames :1
# 33 frames :1
# 34 frames :2
# 36 frames :1
# 38 frames :1
# 39 frames :1
# 44 frames :2
# 64 frames :2
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.04
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 6
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 25.0811
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 6
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 300
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0