Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-10_18.43.34 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "0c93f05778390c8c3e644a0d027c0b25",
"name": "M1_20170618_1451_dsRNA_Q5_col.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "16:43:54"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8123 (max 8124) in '8123'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 25.0449
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 3.87639e+008
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 1050 frames Cell Events: 32 mean_circ: 0.954877
Ran for 1550 frames Cell Events: 42 mean_circ: 0.956147
Ran for 2050 frames Cell Events: 55 mean_circ: 0.958013
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Ran for 4550 frames Cell Events: 113 mean_circ: 0.952477
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Ran for 5550 frames Cell Events: 145 mean_circ: 0.952351
Ran for 6550 frames Cell Events: 180 mean_circ: 0.951116
Ran for 7050 frames Cell Events: 200 mean_circ: 0.95116
Ran for 7550 frames Cell Events: 213 mean_circ: 0.951076
Ran for 8550 frames Cell Events: 253 mean_circ: 0.950769
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Ran for 9550 frames Cell Events: 280 mean_circ: 0.95019
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Ran for 76550 frames Cell Events: 2292 mean_circ: 0.951824
Ran for 77050 frames Cell Events: 2310 mean_circ: 0.951718
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Ran for 87050 frames Cell Events: 2658 mean_circ: 0.951794
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Ran for 92550 frames Cell Events: 2807 mean_circ: 0.951955
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Ran for 96550 frames Cell Events: 2962 mean_circ: 0.951875
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Ran for 100050 frames Cell Events: 3078 mean_circ: 0.951832
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Ran for 122050 frames Cell Events: 3776 mean_circ: 0.951552
Ran for 122550 frames Cell Events: 3794 mean_circ: 0.951501
Ran for 123050 frames Cell Events: 3809 mean_circ: 0.951515
Ran for 123550 frames Cell Events: 3827 mean_circ: 0.951551
Ran for 124050 frames Cell Events: 3841 mean_circ: 0.951596
Ran for 124550 frames Cell Events: 3865 mean_circ: 0.951521
Ran for 125050 frames Cell Events: 3877 mean_circ: 0.951534
Ran for 125550 frames Cell Events: 3886 mean_circ: 0.951548
Ran for 126050 frames Cell Events: 3903 mean_circ: 0.951559
Ran for 126550 frames Cell Events: 3927 mean_circ: 0.951606
Ran for 127050 frames Cell Events: 3938 mean_circ: 0.951583
Ran for 127550 frames Cell Events: 3957 mean_circ: 0.951567
Ran for 128050 frames Cell Events: 3973 mean_circ: 0.951564
Ran for 128550 frames Cell Events: 3993 mean_circ: 0.951572
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Ran for 201050 frames Cell Events: 6482 mean_circ: 0.951765
Ran for 201550 frames Cell Events: 6489 mean_circ: 0.95173
Ran for 203050 frames Cell Events: 6538 mean_circ: 0.951742
Ran for 203550 frames Cell Events: 6549 mean_circ: 0.951767
Ran for 204550 frames Cell Events: 6582 mean_circ: 0.951821
Ran for 205050 frames Cell Events: 6600 mean_circ: 0.951811
Ran for 206550 frames Cell Events: 6650 mean_circ: 0.951799
Ran for 207550 frames Cell Events: 6685 mean_circ: 0.951837
Ran for 208050 frames Cell Events: 6695 mean_circ: 0.951834
Ran for 208550 frames Cell Events: 6712 mean_circ: 0.951873
Ran for 209050 frames Cell Events: 6737 mean_circ: 0.951792
Ran for 209550 frames Cell Events: 6754 mean_circ: 0.951778
Ran for 210550 frames Cell Events: 6799 mean_circ: 0.951809
Ran for 211050 frames Cell Events: 6821 mean_circ: 0.951796
Ran for 211550 frames Cell Events: 6838 mean_circ: 0.951751
Ran for 212050 frames Cell Events: 6850 mean_circ: 0.951748
Ran for 212550 frames Cell Events: 6871 mean_circ: 0.951746
Ran for 213050 frames Cell Events: 6884 mean_circ: 0.951744
Ran for 213550 frames Cell Events: 6900 mean_circ: 0.951764
Ran for 214550 frames Cell Events: 6930 mean_circ: 0.951704
Ran for 215050 frames Cell Events: 6949 mean_circ: 0.951696
Ran for 215550 frames Cell Events: 6963 mean_circ: 0.951673
Ran for 216550 frames Cell Events: 7004 mean_circ: 0.951655
Ran for 217050 frames Cell Events: 7018 mean_circ: 0.95165
Ran for 217550 frames Cell Events: 7036 mean_circ: 0.951664
Ran for 218050 frames Cell Events: 7053 mean_circ: 0.951674
Ran for 218550 frames Cell Events: 7068 mean_circ: 0.951677
Ran for 219050 frames Cell Events: 7081 mean_circ: 0.951699
Ran for 219550 frames Cell Events: 7092 mean_circ: 0.951706
Ran for 220050 frames Cell Events: 7108 mean_circ: 0.951695
Ran for 220550 frames Cell Events: 7129 mean_circ: 0.951674
Ran for 221050 frames Cell Events: 7139 mean_circ: 0.951689
Ran for 222050 frames Cell Events: 7168 mean_circ: 0.951701
Ran for 222550 frames Cell Events: 7184 mean_circ: 0.951717
Ran for 223050 frames Cell Events: 7210 mean_circ: 0.951749
Ran for 223550 frames Cell Events: 7222 mean_circ: 0.951752
Ran for 224050 frames Cell Events: 7239 mean_circ: 0.951765
Ran for 224550 frames Cell Events: 7256 mean_circ: 0.951771
Ran for 225050 frames Cell Events: 7274 mean_circ: 0.951768
Ran for 225550 frames Cell Events: 7298 mean_circ: 0.951768
Ran for 226050 frames Cell Events: 7313 mean_circ: 0.951796
Ran for 227050 frames Cell Events: 7357 mean_circ: 0.951773
Ran for 228050 frames Cell Events: 7391 mean_circ: 0.951789
Ran for 231550 frames Cell Events: 7516 mean_circ: 0.951783
Ran for 232050 frames Cell Events: 7533 mean_circ: 0.951798
Ran for 232550 frames Cell Events: 7552 mean_circ: 0.951785
Ran for 233050 frames Cell Events: 7574 mean_circ: 0.95181
Ran for 233550 frames Cell Events: 7592 mean_circ: 0.951802
Ran for 234050 frames Cell Events: 7608 mean_circ: 0.951801
Ran for 234550 frames Cell Events: 7628 mean_circ: 0.951803
Ran for 235050 frames Cell Events: 7648 mean_circ: 0.951835
Ran for 236050 frames Cell Events: 7693 mean_circ: 0.95181
Ran for 236550 frames Cell Events: 7708 mean_circ: 0.951792
Ran for 238050 frames Cell Events: 7765 mean_circ: 0.951775
Ran for 238550 frames Cell Events: 7781 mean_circ: 0.951784
Ran for 239050 frames Cell Events: 7799 mean_circ: 0.951789
Ran for 240050 frames Cell Events: 7839 mean_circ: 0.951783
Ran for 240550 frames Cell Events: 7846 mean_circ: 0.95175
Ran for 241050 frames Cell Events: 7867 mean_circ: 0.951752
Ran for 241550 frames Cell Events: 7887 mean_circ: 0.951729
Ran for 242050 frames Cell Events: 7901 mean_circ: 0.951691
Ran for 242550 frames Cell Events: 7913 mean_circ: 0.951681
Ran for 244050 frames Cell Events: 7969 mean_circ: 0.951695
Ran for 244550 frames Cell Events: 7991 mean_circ: 0.951704
Ran for 246050 frames Cell Events: 8053 mean_circ: 0.95167
Ran for 246550 frames Cell Events: 8064 mean_circ: 0.951689
Ran for 247050 frames Cell Events: 8079 mean_circ: 0.951705
Ran for 247550 frames Cell Events: 8106 mean_circ: 0.951702
Ran for 248050 frames Cell Events: 8119 mean_circ: 0.951729



End this measurment..



Stopping time: 3.87763e+008
****************************************
Measurement finished

Mean Circularitiy: 0.951733
Mean Area: 582.497
Mean FL-1 intensity: 2920.01
Mean FL-2 intensity: 3479.69
Mean FL-3 intensity: 0

frames processed: 248203
Total Time: 124231ms
Number of Runs (new bg img) 0
Frames collected: 248271
Skipped frames: 13680
Rescued Frames: 1483589
Samples DAQ: 62068108
Samples written: 124135702
Samples per Channel: 62067851
FPS: 1998.46

Samples per second: 499617
Expected samples/s: 500000

Events: 8124
Events per second: 65.3944
jumped back (PMT): 5697
#Frames read, frequency
# 0 frames :350492
# 1 frames :127295
# 2 frames :5665
# 3 frames :1932
# 4 frames :1147
# 5 frames :539
# 6 frames :616
# 7 frames :2690
# 8 frames :1022
# 9 frames :775
# 10 frames :1164
# 11 frames :780
# 12 frames :491
# 13 frames :378
# 14 frames :222
# 15 frames :238
# 16 frames :174
# 17 frames :172
# 18 frames :148
# 19 frames :102
# 20 frames :64
# 21 frames :58
# 22 frames :60
# 23 frames :43
# 24 frames :31
# 25 frames :41
# 26 frames :24
# 27 frames :29
# 28 frames :16
# 29 frames :20
# 30 frames :10
# 31 frames :12
# 32 frames :7
# 33 frames :5
# 34 frames :1
# 35 frames :4
# 36 frames :2
# 37 frames :2
# 39 frames :1
# 40 frames :5
# 42 frames :2
# 45 frames :1
# 48 frames :1
# 68 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.04
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 6
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 25.0449
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 6
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 300
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0