Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-10_14.40.59 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "a8d3e6a6d685c15f6d723828b9c8fc10",
"name": "M1_20170608_2010_c2_dsRNA_R6_col.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "12:41:22"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8903 (max 8904) in '8903'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 25.1728
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 2.11604e+009
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 550 frames Cell Events: 11 mean_circ: 0.939082
Ran for 1550 frames Cell Events: 57 mean_circ: 0.946727
Ran for 2050 frames Cell Events: 69 mean_circ: 0.948007
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Ran for 5050 frames Cell Events: 190 mean_circ: 0.950123
Ran for 6550 frames Cell Events: 237 mean_circ: 0.951304
Ran for 7550 frames Cell Events: 282 mean_circ: 0.950389
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Ran for 11050 frames Cell Events: 429 mean_circ: 0.95162
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Ran for 106050 frames Cell Events: 3506 mean_circ: 0.952333
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Ran for 121550 frames Cell Events: 4100 mean_circ: 0.952177
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Ran for 218550 frames Cell Events: 7577 mean_circ: 0.951572
Ran for 219050 frames Cell Events: 7596 mean_circ: 0.951595
Ran for 219550 frames Cell Events: 7616 mean_circ: 0.951596
Ran for 220050 frames Cell Events: 7625 mean_circ: 0.951619
Ran for 221050 frames Cell Events: 7655 mean_circ: 0.95164
Ran for 221550 frames Cell Events: 7677 mean_circ: 0.951627
Ran for 222050 frames Cell Events: 7692 mean_circ: 0.951638
Ran for 223550 frames Cell Events: 7738 mean_circ: 0.95165
Ran for 224050 frames Cell Events: 7759 mean_circ: 0.951633
Ran for 224550 frames Cell Events: 7783 mean_circ: 0.951641
Ran for 225550 frames Cell Events: 7810 mean_circ: 0.951616
Ran for 226550 frames Cell Events: 7846 mean_circ: 0.951607
Ran for 229050 frames Cell Events: 7974 mean_circ: 0.951601
Ran for 229550 frames Cell Events: 7987 mean_circ: 0.951615
Ran for 231050 frames Cell Events: 8028 mean_circ: 0.951572
Ran for 232050 frames Cell Events: 8056 mean_circ: 0.951591
Ran for 232550 frames Cell Events: 8076 mean_circ: 0.951619
Ran for 233050 frames Cell Events: 8088 mean_circ: 0.951613
Ran for 233550 frames Cell Events: 8098 mean_circ: 0.951614
Ran for 234050 frames Cell Events: 8116 mean_circ: 0.951585
Ran for 234550 frames Cell Events: 8135 mean_circ: 0.951592
Ran for 235050 frames Cell Events: 8153 mean_circ: 0.951584
Ran for 236550 frames Cell Events: 8212 mean_circ: 0.951578
Ran for 237550 frames Cell Events: 8259 mean_circ: 0.951559
Ran for 238050 frames Cell Events: 8270 mean_circ: 0.951558
Ran for 240050 frames Cell Events: 8329 mean_circ: 0.951568
Ran for 241050 frames Cell Events: 8363 mean_circ: 0.951572
Ran for 242050 frames Cell Events: 8399 mean_circ: 0.951603
Ran for 243050 frames Cell Events: 8432 mean_circ: 0.95155
Ran for 244550 frames Cell Events: 8483 mean_circ: 0.951589
Ran for 245050 frames Cell Events: 8498 mean_circ: 0.951606
Ran for 245550 frames Cell Events: 8525 mean_circ: 0.95162
Ran for 246050 frames Cell Events: 8550 mean_circ: 0.951569
Ran for 247050 frames Cell Events: 8574 mean_circ: 0.951571
Ran for 247550 frames Cell Events: 8588 mean_circ: 0.951562
Ran for 248550 frames Cell Events: 8606 mean_circ: 0.951568
Ran for 249050 frames Cell Events: 8619 mean_circ: 0.951569
Ran for 250550 frames Cell Events: 8669 mean_circ: 0.951522
Ran for 251550 frames Cell Events: 8710 mean_circ: 0.951512
Ran for 252550 frames Cell Events: 8738 mean_circ: 0.951526
Ran for 253050 frames Cell Events: 8764 mean_circ: 0.951532
Ran for 253550 frames Cell Events: 8780 mean_circ: 0.951526
Ran for 255050 frames Cell Events: 8853 mean_circ: 0.951548
Ran for 255550 frames Cell Events: 8868 mean_circ: 0.951545



End this measurment..



Stopping time: 2.11616e+009
****************************************
Measurement finished

Mean Circularitiy: 0.951553
Mean Area: 638.901
Mean FL-1 intensity: 4024.18
Mean FL-2 intensity: 4969.34
Mean FL-3 intensity: 0

frames processed: 255279
Total Time: 128318ms
Number of Runs (new bg img) 0
Frames collected: 256540
Skipped frames: 32287
Rescued Frames: 2950442
Samples DAQ: 64134920
Samples written: 128269532
Samples per Channel: 64134766
FPS: 1999.24

Samples per second: 499809
Expected samples/s: 500000

Events: 8904
Events per second: 69.3899
jumped back (PMT): 4502
#Frames read, frequency
# 0 frames :377447
# 1 frames :116919
# 2 frames :4680
# 3 frames :1975
# 4 frames :1253
# 5 frames :708
# 6 frames :772
# 7 frames :1874
# 8 frames :1216
# 9 frames :970
# 10 frames :1120
# 11 frames :748
# 12 frames :580
# 13 frames :418
# 14 frames :278
# 15 frames :260
# 16 frames :273
# 17 frames :248
# 18 frames :216
# 19 frames :203
# 20 frames :177
# 21 frames :124
# 22 frames :101
# 23 frames :65
# 24 frames :65
# 25 frames :42
# 26 frames :40
# 27 frames :34
# 28 frames :38
# 29 frames :26
# 30 frames :20
# 31 frames :16
# 32 frames :19
# 33 frames :23
# 34 frames :17
# 35 frames :10
# 36 frames :13
# 37 frames :7
# 38 frames :4
# 39 frames :9
# 40 frames :7
# 41 frames :2
# 43 frames :7
# 44 frames :2
# 45 frames :4
# 46 frames :2
# 47 frames :5
# 48 frames :1
# 49 frames :2
# 50 frames :2
# 51 frames :3
# 52 frames :1
# 53 frames :1
# 54 frames :1
# 55 frames :2
# 57 frames :4
# 58 frames :2
# 60 frames :2
# 64 frames :1
# 65 frames :1
# 72 frames :1
# 81 frames :1
# 93 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.04
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 6
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 25.1728
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 6
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 300
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0