Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-10_16.35.56 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "d5463498eb1e59e055c119556a05bd83",
"name": "M1_20170612_1441_dsRNA_S3_col.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "14:36:18"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8640 (max 8641) in '8640'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 24.7167
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 2.44187e+009
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 2050 frames Cell Events: 115 mean_circ: 0.944003
Ran for 2550 frames Cell Events: 143 mean_circ: 0.942839
Ran for 3050 frames Cell Events: 167 mean_circ: 0.943027
Ran for 5050 frames Cell Events: 293 mean_circ: 0.941394
Ran for 6550 frames Cell Events: 407 mean_circ: 0.941908
Ran for 7550 frames Cell Events: 485 mean_circ: 0.942131
Ran for 10050 frames Cell Events: 625 mean_circ: 0.943456
Ran for 10550 frames Cell Events: 664 mean_circ: 0.943425
Ran for 11550 frames Cell Events: 738 mean_circ: 0.94362
Ran for 12050 frames Cell Events: 762 mean_circ: 0.943279
Ran for 12550 frames Cell Events: 798 mean_circ: 0.943335
Ran for 14050 frames Cell Events: 896 mean_circ: 0.942228
Ran for 15550 frames Cell Events: 996 mean_circ: 0.941484
Ran for 16050 frames Cell Events: 1034 mean_circ: 0.941484
Ran for 16550 frames Cell Events: 1068 mean_circ: 0.941811
Ran for 17550 frames Cell Events: 1128 mean_circ: 0.942174
Ran for 18050 frames Cell Events: 1164 mean_circ: 0.942095
Ran for 18550 frames Cell Events: 1180 mean_circ: 0.942185
Ran for 19050 frames Cell Events: 1203 mean_circ: 0.942298
Ran for 20050 frames Cell Events: 1277 mean_circ: 0.942789
Ran for 20550 frames Cell Events: 1312 mean_circ: 0.942526
Ran for 21550 frames Cell Events: 1362 mean_circ: 0.942821
Ran for 22550 frames Cell Events: 1421 mean_circ: 0.94261
Ran for 23550 frames Cell Events: 1470 mean_circ: 0.942661
Ran for 24050 frames Cell Events: 1497 mean_circ: 0.942784
Ran for 24550 frames Cell Events: 1521 mean_circ: 0.942931
Ran for 26550 frames Cell Events: 1627 mean_circ: 0.942758
Ran for 27050 frames Cell Events: 1659 mean_circ: 0.942653
Ran for 28050 frames Cell Events: 1700 mean_circ: 0.942849
Ran for 28550 frames Cell Events: 1727 mean_circ: 0.942873
Ran for 30050 frames Cell Events: 1822 mean_circ: 0.942947
Ran for 30550 frames Cell Events: 1840 mean_circ: 0.943051
Ran for 34550 frames Cell Events: 2158 mean_circ: 0.943332
Ran for 35550 frames Cell Events: 2235 mean_circ: 0.94341
Ran for 36550 frames Cell Events: 2291 mean_circ: 0.943434
Ran for 37050 frames Cell Events: 2332 mean_circ: 0.943421
Ran for 39050 frames Cell Events: 2487 mean_circ: 0.943418
Ran for 39550 frames Cell Events: 2528 mean_circ: 0.943454
Ran for 41550 frames Cell Events: 2623 mean_circ: 0.943442
Ran for 42050 frames Cell Events: 2638 mean_circ: 0.94348
Ran for 43050 frames Cell Events: 2697 mean_circ: 0.943538
Ran for 44550 frames Cell Events: 2802 mean_circ: 0.943631
Ran for 45050 frames Cell Events: 2833 mean_circ: 0.943545
Ran for 45550 frames Cell Events: 2859 mean_circ: 0.943587
Ran for 46050 frames Cell Events: 2889 mean_circ: 0.943527
Ran for 46550 frames Cell Events: 2908 mean_circ: 0.943519
Ran for 47050 frames Cell Events: 2926 mean_circ: 0.943568
Ran for 47550 frames Cell Events: 2955 mean_circ: 0.943608
Ran for 48050 frames Cell Events: 2987 mean_circ: 0.943621
Ran for 48550 frames Cell Events: 3011 mean_circ: 0.943674
Ran for 50050 frames Cell Events: 3092 mean_circ: 0.943806
Ran for 51050 frames Cell Events: 3157 mean_circ: 0.943567
Ran for 51550 frames Cell Events: 3186 mean_circ: 0.943507
Ran for 52050 frames Cell Events: 3221 mean_circ: 0.943531
Ran for 52550 frames Cell Events: 3245 mean_circ: 0.943553
Ran for 53050 frames Cell Events: 3281 mean_circ: 0.943451
Ran for 54550 frames Cell Events: 3362 mean_circ: 0.943423
Ran for 55050 frames Cell Events: 3381 mean_circ: 0.943475
Ran for 55550 frames Cell Events: 3413 mean_circ: 0.943512
Ran for 56550 frames Cell Events: 3492 mean_circ: 0.94346
Ran for 57050 frames Cell Events: 3514 mean_circ: 0.943362
Ran for 58550 frames Cell Events: 3610 mean_circ: 0.943145
Ran for 59550 frames Cell Events: 3676 mean_circ: 0.943071
Ran for 61050 frames Cell Events: 3796 mean_circ: 0.94312
Ran for 61550 frames Cell Events: 3817 mean_circ: 0.943246
Ran for 62550 frames Cell Events: 3873 mean_circ: 0.943207
Ran for 63050 frames Cell Events: 3897 mean_circ: 0.943203
Ran for 63550 frames Cell Events: 3916 mean_circ: 0.943116
Ran for 66050 frames Cell Events: 4047 mean_circ: 0.943224
Ran for 66550 frames Cell Events: 4084 mean_circ: 0.943157
Ran for 68050 frames Cell Events: 4176 mean_circ: 0.943204
Ran for 69550 frames Cell Events: 4278 mean_circ: 0.943236
Ran for 70050 frames Cell Events: 4307 mean_circ: 0.943253
Ran for 70550 frames Cell Events: 4332 mean_circ: 0.943291
Ran for 71550 frames Cell Events: 4401 mean_circ: 0.943179
Ran for 72050 frames Cell Events: 4429 mean_circ: 0.943194
Ran for 73050 frames Cell Events: 4494 mean_circ: 0.943101
Ran for 74550 frames Cell Events: 4619 mean_circ: 0.943134
Ran for 75550 frames Cell Events: 4681 mean_circ: 0.943068
Ran for 76550 frames Cell Events: 4720 mean_circ: 0.943124
Ran for 77050 frames Cell Events: 4746 mean_circ: 0.943139
Ran for 80550 frames Cell Events: 4997 mean_circ: 0.943289
Ran for 81550 frames Cell Events: 5061 mean_circ: 0.943263
Ran for 82050 frames Cell Events: 5086 mean_circ: 0.943189
Ran for 82550 frames Cell Events: 5120 mean_circ: 0.943167
Ran for 83050 frames Cell Events: 5146 mean_circ: 0.943224
Ran for 84550 frames Cell Events: 5269 mean_circ: 0.943352
Ran for 85050 frames Cell Events: 5320 mean_circ: 0.943352
Ran for 85550 frames Cell Events: 5354 mean_circ: 0.943486
Ran for 86550 frames Cell Events: 5417 mean_circ: 0.943602
Ran for 88050 frames Cell Events: 5526 mean_circ: 0.943732
Ran for 90050 frames Cell Events: 5662 mean_circ: 0.943632
Ran for 91050 frames Cell Events: 5732 mean_circ: 0.943573
Ran for 92050 frames Cell Events: 5778 mean_circ: 0.943564
Ran for 93050 frames Cell Events: 5833 mean_circ: 0.943646
Ran for 93550 frames Cell Events: 5858 mean_circ: 0.943656
Ran for 96050 frames Cell Events: 6048 mean_circ: 0.943635
Ran for 97050 frames Cell Events: 6109 mean_circ: 0.943687
Ran for 97550 frames Cell Events: 6124 mean_circ: 0.943698
Ran for 98050 frames Cell Events: 6152 mean_circ: 0.943734
Ran for 98550 frames Cell Events: 6177 mean_circ: 0.943771
Ran for 99550 frames Cell Events: 6212 mean_circ: 0.943783
Ran for 100550 frames Cell Events: 6259 mean_circ: 0.943698
Ran for 101050 frames Cell Events: 6294 mean_circ: 0.943758
Ran for 104050 frames Cell Events: 6493 mean_circ: 0.94396
Ran for 104550 frames Cell Events: 6515 mean_circ: 0.943947
Ran for 107550 frames Cell Events: 6721 mean_circ: 0.94393
Ran for 108050 frames Cell Events: 6746 mean_circ: 0.943954
Ran for 109550 frames Cell Events: 6830 mean_circ: 0.943937
Ran for 110050 frames Cell Events: 6854 mean_circ: 0.943949
Ran for 110550 frames Cell Events: 6868 mean_circ: 0.943935
Ran for 114050 frames Cell Events: 7166 mean_circ: 0.943913
Ran for 116550 frames Cell Events: 7357 mean_circ: 0.943789
Ran for 119550 frames Cell Events: 7572 mean_circ: 0.943872
Ran for 121050 frames Cell Events: 7658 mean_circ: 0.943885
Ran for 121550 frames Cell Events: 7690 mean_circ: 0.943888
Ran for 122050 frames Cell Events: 7719 mean_circ: 0.943907
Ran for 123050 frames Cell Events: 7780 mean_circ: 0.943858
Ran for 124050 frames Cell Events: 7864 mean_circ: 0.943881
Ran for 124550 frames Cell Events: 7889 mean_circ: 0.943761
Ran for 125050 frames Cell Events: 7912 mean_circ: 0.943742
Ran for 126050 frames Cell Events: 7973 mean_circ: 0.943632
Ran for 127050 frames Cell Events: 8046 mean_circ: 0.943631
Ran for 129550 frames Cell Events: 8230 mean_circ: 0.943646
Ran for 133050 frames Cell Events: 8506 mean_circ: 0.943635
Ran for 133550 frames Cell Events: 8541 mean_circ: 0.943635
Ran for 134050 frames Cell Events: 8567 mean_circ: 0.943659
Ran for 135050 frames Cell Events: 8627 mean_circ: 0.943688



End this measurment..



Stopping time: 2.44194e+009
****************************************
Measurement finished

Mean Circularitiy: 0.943692
Mean Area: 601.332
Mean FL-1 intensity: 3038.77
Mean FL-2 intensity: 3378.32
Mean FL-3 intensity: 0

frames processed: 132570
Total Time: 67730.3ms
Number of Runs (new bg img) 0
Frames collected: 135303
Skipped frames: 11678
Rescued Frames: 1571578
Samples DAQ: 33827730
Samples written: 67651448
Samples per Channel: 33825724
FPS: 1997.67

Samples per second: 499418
Expected samples/s: 500000

Events: 8641
Events per second: 127.58
jumped back (PMT): 3006
#Frames read, frequency
# 0 frames :215385
# 1 frames :44538
# 2 frames :2291
# 3 frames :900
# 4 frames :471
# 5 frames :257
# 6 frames :1464
# 7 frames :814
# 8 frames :725
# 9 frames :464
# 10 frames :689
# 11 frames :306
# 12 frames :265
# 13 frames :146
# 14 frames :275
# 15 frames :218
# 16 frames :180
# 17 frames :136
# 18 frames :95
# 19 frames :70
# 20 frames :90
# 21 frames :76
# 22 frames :74
# 23 frames :67
# 24 frames :50
# 25 frames :39
# 26 frames :43
# 27 frames :40
# 28 frames :30
# 29 frames :31
# 30 frames :26
# 31 frames :25
# 32 frames :25
# 33 frames :19
# 34 frames :19
# 35 frames :11
# 36 frames :14
# 37 frames :21
# 38 frames :14
# 39 frames :16
# 40 frames :12
# 41 frames :5
# 42 frames :10
# 43 frames :12
# 44 frames :5
# 45 frames :5
# 46 frames :6
# 47 frames :6
# 48 frames :4
# 49 frames :6
# 50 frames :4
# 51 frames :7
# 52 frames :3
# 53 frames :5
# 54 frames :6
# 55 frames :3
# 56 frames :4
# 57 frames :1
# 58 frames :1
# 59 frames :3
# 60 frames :2
# 61 frames :3
# 63 frames :1
# 65 frames :1
# 66 frames :1
# 68 frames :1
# 71 frames :1
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# 75 frames :1
# 78 frames :1
# 87 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.04
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 6
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 24.7167
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 6
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 300
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0