Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-10_17.00.10 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "d801c28fb73f961a44b9c6a37d95a67f",
"name": "M1_20170612_2036_dsRNA_U1_col.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "15:00:31"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8291 (max 8293) in '8291'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 24.9623
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 2.46318e+009
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 550 frames Cell Events: 18 mean_circ: 0.8643
Ran for 1050 frames Cell Events: 30 mean_circ: 0.905901
Ran for 2050 frames Cell Events: 69 mean_circ: 0.932527
Ran for 2550 frames Cell Events: 85 mean_circ: 0.933089
Ran for 4550 frames Cell Events: 187 mean_circ: 0.940506
Ran for 5050 frames Cell Events: 216 mean_circ: 0.941318
Ran for 5550 frames Cell Events: 250 mean_circ: 0.942852
Ran for 6050 frames Cell Events: 264 mean_circ: 0.94368
Ran for 9550 frames Cell Events: 444 mean_circ: 0.946184
Ran for 14050 frames Cell Events: 703 mean_circ: 0.946424
Ran for 15050 frames Cell Events: 761 mean_circ: 0.946432
Ran for 16050 frames Cell Events: 783 mean_circ: 0.946663
Ran for 16550 frames Cell Events: 809 mean_circ: 0.946839
Ran for 17550 frames Cell Events: 869 mean_circ: 0.947025
Ran for 20050 frames Cell Events: 1003 mean_circ: 0.946043
Ran for 20550 frames Cell Events: 1013 mean_circ: 0.94614
Ran for 21050 frames Cell Events: 1036 mean_circ: 0.946251
Ran for 22050 frames Cell Events: 1075 mean_circ: 0.946305
Ran for 22550 frames Cell Events: 1099 mean_circ: 0.946335
Ran for 23050 frames Cell Events: 1117 mean_circ: 0.946413
Ran for 23550 frames Cell Events: 1133 mean_circ: 0.946469
Ran for 25050 frames Cell Events: 1195 mean_circ: 0.946977
Ran for 26050 frames Cell Events: 1238 mean_circ: 0.946918
Ran for 26550 frames Cell Events: 1256 mean_circ: 0.947085
Ran for 27050 frames Cell Events: 1275 mean_circ: 0.947266
Ran for 29050 frames Cell Events: 1336 mean_circ: 0.947553
Ran for 30050 frames Cell Events: 1383 mean_circ: 0.947803
Ran for 31050 frames Cell Events: 1436 mean_circ: 0.947925
Ran for 32050 frames Cell Events: 1477 mean_circ: 0.947943
Ran for 33050 frames Cell Events: 1528 mean_circ: 0.948255
Ran for 33550 frames Cell Events: 1556 mean_circ: 0.948012
Ran for 36050 frames Cell Events: 1683 mean_circ: 0.947423
Ran for 37050 frames Cell Events: 1715 mean_circ: 0.947385
Ran for 40050 frames Cell Events: 1815 mean_circ: 0.947175
Ran for 41050 frames Cell Events: 1875 mean_circ: 0.946827
Ran for 42050 frames Cell Events: 1934 mean_circ: 0.946746
Ran for 42550 frames Cell Events: 1957 mean_circ: 0.946635
Ran for 43050 frames Cell Events: 1981 mean_circ: 0.946509
Ran for 43550 frames Cell Events: 2008 mean_circ: 0.946364
Ran for 44050 frames Cell Events: 2036 mean_circ: 0.946435
Ran for 45550 frames Cell Events: 2135 mean_circ: 0.946449
Ran for 46050 frames Cell Events: 2159 mean_circ: 0.946355
Ran for 46550 frames Cell Events: 2183 mean_circ: 0.946308
Ran for 47050 frames Cell Events: 2213 mean_circ: 0.946282
Ran for 48050 frames Cell Events: 2255 mean_circ: 0.946293
Ran for 49050 frames Cell Events: 2303 mean_circ: 0.946392
Ran for 49550 frames Cell Events: 2327 mean_circ: 0.946287
Ran for 50050 frames Cell Events: 2344 mean_circ: 0.946153
Ran for 50550 frames Cell Events: 2361 mean_circ: 0.946175
Ran for 52550 frames Cell Events: 2442 mean_circ: 0.946072
Ran for 54050 frames Cell Events: 2525 mean_circ: 0.946125
Ran for 54550 frames Cell Events: 2545 mean_circ: 0.946119
Ran for 56550 frames Cell Events: 2625 mean_circ: 0.946182
Ran for 57550 frames Cell Events: 2679 mean_circ: 0.94627
Ran for 59550 frames Cell Events: 2788 mean_circ: 0.946099
Ran for 60050 frames Cell Events: 2816 mean_circ: 0.946081
Ran for 62050 frames Cell Events: 2907 mean_circ: 0.946194
Ran for 64550 frames Cell Events: 3030 mean_circ: 0.946036
Ran for 65050 frames Cell Events: 3050 mean_circ: 0.94599
Ran for 66050 frames Cell Events: 3114 mean_circ: 0.945863
Ran for 67550 frames Cell Events: 3187 mean_circ: 0.946013
Ran for 68050 frames Cell Events: 3209 mean_circ: 0.946067
Ran for 70050 frames Cell Events: 3312 mean_circ: 0.945987
Ran for 70550 frames Cell Events: 3339 mean_circ: 0.946063
Ran for 72550 frames Cell Events: 3454 mean_circ: 0.946218
Ran for 74050 frames Cell Events: 3523 mean_circ: 0.946228
Ran for 74550 frames Cell Events: 3555 mean_circ: 0.946244
Ran for 75050 frames Cell Events: 3578 mean_circ: 0.946311
Ran for 75550 frames Cell Events: 3604 mean_circ: 0.946394
Ran for 76050 frames Cell Events: 3636 mean_circ: 0.946474
Ran for 77550 frames Cell Events: 3715 mean_circ: 0.946323
Ran for 78050 frames Cell Events: 3739 mean_circ: 0.946344
Ran for 79550 frames Cell Events: 3811 mean_circ: 0.946471
Ran for 80050 frames Cell Events: 3829 mean_circ: 0.946346
Ran for 80550 frames Cell Events: 3854 mean_circ: 0.946454
Ran for 81050 frames Cell Events: 3878 mean_circ: 0.946456
Ran for 81550 frames Cell Events: 3896 mean_circ: 0.946489
Ran for 82550 frames Cell Events: 3945 mean_circ: 0.946413
Ran for 83050 frames Cell Events: 3958 mean_circ: 0.946361
Ran for 83550 frames Cell Events: 3980 mean_circ: 0.94641
Ran for 84550 frames Cell Events: 4028 mean_circ: 0.946336
Ran for 85050 frames Cell Events: 4048 mean_circ: 0.946319
Ran for 88050 frames Cell Events: 4204 mean_circ: 0.94638
Ran for 88550 frames Cell Events: 4235 mean_circ: 0.946384
Ran for 89550 frames Cell Events: 4303 mean_circ: 0.9464
Ran for 90550 frames Cell Events: 4377 mean_circ: 0.946127
Ran for 91550 frames Cell Events: 4421 mean_circ: 0.94616
Ran for 93550 frames Cell Events: 4526 mean_circ: 0.946092
Ran for 94050 frames Cell Events: 4550 mean_circ: 0.94614
Ran for 95050 frames Cell Events: 4604 mean_circ: 0.946112
Ran for 95550 frames Cell Events: 4629 mean_circ: 0.946155
Ran for 97050 frames Cell Events: 4694 mean_circ: 0.94605
Ran for 97550 frames Cell Events: 4711 mean_circ: 0.946122
Ran for 99050 frames Cell Events: 4781 mean_circ: 0.946231
Ran for 99550 frames Cell Events: 4795 mean_circ: 0.946256
Ran for 100550 frames Cell Events: 4829 mean_circ: 0.94625
Ran for 102050 frames Cell Events: 4887 mean_circ: 0.946356
Ran for 104050 frames Cell Events: 4975 mean_circ: 0.94647
Ran for 105050 frames Cell Events: 5022 mean_circ: 0.946379
Ran for 105550 frames Cell Events: 5048 mean_circ: 0.946405
Ran for 106550 frames Cell Events: 5080 mean_circ: 0.946452
Ran for 108050 frames Cell Events: 5169 mean_circ: 0.946421
Ran for 108550 frames Cell Events: 5188 mean_circ: 0.946452
Ran for 109050 frames Cell Events: 5219 mean_circ: 0.946491
Ran for 110050 frames Cell Events: 5258 mean_circ: 0.94649
Ran for 111050 frames Cell Events: 5295 mean_circ: 0.946469
Ran for 111550 frames Cell Events: 5312 mean_circ: 0.94651
Ran for 112550 frames Cell Events: 5352 mean_circ: 0.946439
Ran for 113050 frames Cell Events: 5378 mean_circ: 0.94646
Ran for 113550 frames Cell Events: 5400 mean_circ: 0.946474
Ran for 114050 frames Cell Events: 5426 mean_circ: 0.94649
Ran for 114550 frames Cell Events: 5447 mean_circ: 0.946385
Ran for 115050 frames Cell Events: 5453 mean_circ: 0.94638
Ran for 116550 frames Cell Events: 5507 mean_circ: 0.946429
Ran for 121050 frames Cell Events: 5730 mean_circ: 0.94627
Ran for 121550 frames Cell Events: 5757 mean_circ: 0.94627
Ran for 122050 frames Cell Events: 5772 mean_circ: 0.94627
Ran for 122550 frames Cell Events: 5796 mean_circ: 0.946254
Ran for 123550 frames Cell Events: 5846 mean_circ: 0.946276
Ran for 126050 frames Cell Events: 5994 mean_circ: 0.946047
Ran for 127050 frames Cell Events: 6044 mean_circ: 0.946006
Ran for 127550 frames Cell Events: 6073 mean_circ: 0.945987
Ran for 128550 frames Cell Events: 6133 mean_circ: 0.946039
Ran for 129550 frames Cell Events: 6174 mean_circ: 0.946071
Ran for 130550 frames Cell Events: 6236 mean_circ: 0.946094
Ran for 131050 frames Cell Events: 6260 mean_circ: 0.946104
Ran for 131550 frames Cell Events: 6283 mean_circ: 0.946149
Ran for 132550 frames Cell Events: 6336 mean_circ: 0.946187
Ran for 133050 frames Cell Events: 6362 mean_circ: 0.946217
Ran for 134050 frames Cell Events: 6406 mean_circ: 0.946167
Ran for 136050 frames Cell Events: 6499 mean_circ: 0.946213
Ran for 136550 frames Cell Events: 6529 mean_circ: 0.946222
Ran for 137550 frames Cell Events: 6581 mean_circ: 0.946189
Ran for 138050 frames Cell Events: 6612 mean_circ: 0.946152
Ran for 139050 frames Cell Events: 6665 mean_circ: 0.946015
Ran for 139550 frames Cell Events: 6679 mean_circ: 0.946041
Ran for 140050 frames Cell Events: 6721 mean_circ: 0.945976
Ran for 141050 frames Cell Events: 6780 mean_circ: 0.945875
Ran for 141550 frames Cell Events: 6817 mean_circ: 0.945805
Ran for 142050 frames Cell Events: 6847 mean_circ: 0.945786
Ran for 143550 frames Cell Events: 6938 mean_circ: 0.945708
Ran for 146550 frames Cell Events: 7093 mean_circ: 0.945819
Ran for 147050 frames Cell Events: 7125 mean_circ: 0.945815
Ran for 147550 frames Cell Events: 7140 mean_circ: 0.945816
Ran for 148550 frames Cell Events: 7197 mean_circ: 0.94579
Ran for 149050 frames Cell Events: 7226 mean_circ: 0.945799
Ran for 150050 frames Cell Events: 7272 mean_circ: 0.945826
Ran for 151050 frames Cell Events: 7325 mean_circ: 0.945869
Ran for 151550 frames Cell Events: 7349 mean_circ: 0.945914
Ran for 152550 frames Cell Events: 7387 mean_circ: 0.945908
Ran for 153050 frames Cell Events: 7405 mean_circ: 0.945922
Ran for 154050 frames Cell Events: 7449 mean_circ: 0.945948
Ran for 154550 frames Cell Events: 7487 mean_circ: 0.945948
Ran for 156550 frames Cell Events: 7590 mean_circ: 0.946045
Ran for 157050 frames Cell Events: 7618 mean_circ: 0.94609
Ran for 157550 frames Cell Events: 7630 mean_circ: 0.946083
Ran for 158550 frames Cell Events: 7697 mean_circ: 0.946167
Ran for 159050 frames Cell Events: 7711 mean_circ: 0.946181
Ran for 159550 frames Cell Events: 7737 mean_circ: 0.946186
Ran for 160050 frames Cell Events: 7756 mean_circ: 0.946175
Ran for 162550 frames Cell Events: 7880 mean_circ: 0.946248
Ran for 163550 frames Cell Events: 7921 mean_circ: 0.946238
Ran for 164050 frames Cell Events: 7948 mean_circ: 0.94627
Ran for 165550 frames Cell Events: 8050 mean_circ: 0.946348
Ran for 166050 frames Cell Events: 8090 mean_circ: 0.946405
Ran for 166550 frames Cell Events: 8107 mean_circ: 0.946395
Ran for 167050 frames Cell Events: 8140 mean_circ: 0.946345
Ran for 168550 frames Cell Events: 8221 mean_circ: 0.946361



End this measurment..



Stopping time: 2.46326e+009
****************************************
Measurement finished

Mean Circularitiy: 0.946328
Mean Area: 622.513
Mean FL-1 intensity: 2962.69
Mean FL-2 intensity: 3503.31
Mean FL-3 intensity: 0

frames processed: 165804
Total Time: 84932.5ms
Number of Runs (new bg img) 0
Frames collected: 169753
Skipped frames: 42066
Rescued Frames: 4527078
Samples DAQ: 42438208
Samples written: 84876298
Samples per Channel: 42438149
FPS: 1998.68

Samples per second: 499669
Expected samples/s: 500000

Events: 8293
Events per second: 97.6423
jumped back (PMT): 3858
#Frames read, frequency
# 0 frames :264505
# 1 frames :61165
# 2 frames :3405
# 3 frames :1372
# 4 frames :782
# 5 frames :412
# 6 frames :1189
# 7 frames :1368
# 8 frames :886
# 9 frames :629
# 10 frames :735
# 11 frames :527
# 12 frames :374
# 13 frames :253
# 14 frames :219
# 15 frames :205
# 16 frames :200
# 17 frames :170
# 18 frames :159
# 19 frames :118
# 20 frames :90
# 21 frames :93
# 22 frames :70
# 23 frames :69
# 24 frames :52
# 25 frames :55
# 26 frames :44
# 27 frames :40
# 28 frames :27
# 29 frames :23
# 30 frames :24
# 31 frames :22
# 32 frames :17
# 33 frames :13
# 34 frames :20
# 35 frames :9
# 36 frames :11
# 37 frames :13
# 38 frames :8
# 39 frames :16
# 40 frames :7
# 41 frames :9
# 42 frames :9
# 43 frames :8
# 44 frames :3
# 45 frames :5
# 46 frames :8
# 47 frames :4
# 48 frames :1
# 50 frames :3
# 51 frames :1
# 52 frames :6
# 53 frames :4
# 54 frames :3
# 55 frames :3
# 56 frames :3
# 58 frames :2
# 59 frames :2
# 60 frames :2
# 61 frames :1
# 65 frames :4
# 66 frames :2
# 67 frames :1
# 68 frames :1
# 76 frames :1
# 80 frames :1
# 81 frames :1
# 95 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.04
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 6
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 24.9623
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 6
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 300
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0