Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-10_19.11.11 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "5bacc99f5dd623c410f6831df6f27e7c",
"name": "M1_20170619_1409_dsRNA_V4_col.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "17:11:33"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8643 (max 8644) in '8643'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 25.0721
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 4.71487e+008
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 550 frames Cell Events: 11 mean_circ: 0.9667
Ran for 1050 frames Cell Events: 32 mean_circ: 0.948506
Ran for 1550 frames Cell Events: 48 mean_circ: 0.951741
Ran for 2050 frames Cell Events: 60 mean_circ: 0.952743
Ran for 2550 frames Cell Events: 81 mean_circ: 0.952537
Ran for 4050 frames Cell Events: 146 mean_circ: 0.954242
Ran for 6050 frames Cell Events: 251 mean_circ: 0.952341
Ran for 6550 frames Cell Events: 266 mean_circ: 0.9522
Ran for 7550 frames Cell Events: 315 mean_circ: 0.952154
Ran for 8550 frames Cell Events: 353 mean_circ: 0.951718
Ran for 9550 frames Cell Events: 380 mean_circ: 0.951879
Ran for 11050 frames Cell Events: 450 mean_circ: 0.951496
Ran for 12050 frames Cell Events: 507 mean_circ: 0.951545
Ran for 13550 frames Cell Events: 585 mean_circ: 0.951515
Ran for 14550 frames Cell Events: 618 mean_circ: 0.951929
Ran for 15050 frames Cell Events: 636 mean_circ: 0.951869
Ran for 16550 frames Cell Events: 721 mean_circ: 0.952029
Ran for 17050 frames Cell Events: 746 mean_circ: 0.95203
Ran for 17550 frames Cell Events: 768 mean_circ: 0.951929
Ran for 20050 frames Cell Events: 877 mean_circ: 0.95201
Ran for 20550 frames Cell Events: 891 mean_circ: 0.951916
Ran for 23550 frames Cell Events: 1038 mean_circ: 0.951331
Ran for 24550 frames Cell Events: 1080 mean_circ: 0.951408
Ran for 27050 frames Cell Events: 1195 mean_circ: 0.951296
Ran for 27550 frames Cell Events: 1212 mean_circ: 0.951286
Ran for 28550 frames Cell Events: 1237 mean_circ: 0.951251
Ran for 29550 frames Cell Events: 1276 mean_circ: 0.951322
Ran for 30050 frames Cell Events: 1303 mean_circ: 0.951258
Ran for 30550 frames Cell Events: 1321 mean_circ: 0.950865
Ran for 32050 frames Cell Events: 1401 mean_circ: 0.95103
Ran for 32550 frames Cell Events: 1433 mean_circ: 0.950734
Ran for 33550 frames Cell Events: 1487 mean_circ: 0.950411
Ran for 34050 frames Cell Events: 1515 mean_circ: 0.950394
Ran for 34550 frames Cell Events: 1553 mean_circ: 0.950505
Ran for 35550 frames Cell Events: 1611 mean_circ: 0.950151
Ran for 36550 frames Cell Events: 1656 mean_circ: 0.950214
Ran for 37050 frames Cell Events: 1675 mean_circ: 0.950455
Ran for 38050 frames Cell Events: 1738 mean_circ: 0.950529
Ran for 38550 frames Cell Events: 1758 mean_circ: 0.950434
Ran for 40050 frames Cell Events: 1824 mean_circ: 0.95075
Ran for 40550 frames Cell Events: 1852 mean_circ: 0.950724
Ran for 41050 frames Cell Events: 1868 mean_circ: 0.950827
Ran for 42550 frames Cell Events: 1944 mean_circ: 0.950771
Ran for 43550 frames Cell Events: 2000 mean_circ: 0.950865
Ran for 44050 frames Cell Events: 2024 mean_circ: 0.950657
Ran for 45050 frames Cell Events: 2074 mean_circ: 0.950603
Ran for 45550 frames Cell Events: 2099 mean_circ: 0.950462
Ran for 46050 frames Cell Events: 2139 mean_circ: 0.950462
Ran for 47050 frames Cell Events: 2202 mean_circ: 0.950528
Ran for 48050 frames Cell Events: 2253 mean_circ: 0.95046
Ran for 50050 frames Cell Events: 2382 mean_circ: 0.950546
Ran for 50550 frames Cell Events: 2419 mean_circ: 0.950477
Ran for 53050 frames Cell Events: 2586 mean_circ: 0.950227
Ran for 55050 frames Cell Events: 2714 mean_circ: 0.950345
Ran for 55550 frames Cell Events: 2738 mean_circ: 0.950351
Ran for 56050 frames Cell Events: 2766 mean_circ: 0.950272
Ran for 57050 frames Cell Events: 2808 mean_circ: 0.950081
Ran for 57550 frames Cell Events: 2826 mean_circ: 0.950035
Ran for 58550 frames Cell Events: 2877 mean_circ: 0.95006
Ran for 59550 frames Cell Events: 2954 mean_circ: 0.950037
Ran for 60050 frames Cell Events: 2980 mean_circ: 0.950084
Ran for 60550 frames Cell Events: 2998 mean_circ: 0.950103
Ran for 61050 frames Cell Events: 3033 mean_circ: 0.950058
Ran for 61550 frames Cell Events: 3070 mean_circ: 0.950062
Ran for 62550 frames Cell Events: 3113 mean_circ: 0.950022
Ran for 63550 frames Cell Events: 3144 mean_circ: 0.950012
Ran for 66050 frames Cell Events: 3299 mean_circ: 0.94975
Ran for 66550 frames Cell Events: 3324 mean_circ: 0.949823
Ran for 67050 frames Cell Events: 3342 mean_circ: 0.949851
Ran for 67550 frames Cell Events: 3353 mean_circ: 0.949836
Ran for 68550 frames Cell Events: 3407 mean_circ: 0.949852
Ran for 69550 frames Cell Events: 3452 mean_circ: 0.949902
Ran for 70550 frames Cell Events: 3507 mean_circ: 0.949959
Ran for 72550 frames Cell Events: 3628 mean_circ: 0.949999
Ran for 75550 frames Cell Events: 3807 mean_circ: 0.950268
Ran for 76550 frames Cell Events: 3851 mean_circ: 0.950331
Ran for 77050 frames Cell Events: 3870 mean_circ: 0.95035
Ran for 77550 frames Cell Events: 3881 mean_circ: 0.950375
Ran for 78050 frames Cell Events: 3901 mean_circ: 0.950352
Ran for 79050 frames Cell Events: 3947 mean_circ: 0.950441
Ran for 81050 frames Cell Events: 4044 mean_circ: 0.950581
Ran for 81550 frames Cell Events: 4068 mean_circ: 0.950616
Ran for 83050 frames Cell Events: 4166 mean_circ: 0.950598
Ran for 83550 frames Cell Events: 4182 mean_circ: 0.950612
Ran for 85550 frames Cell Events: 4267 mean_circ: 0.950497
Ran for 86050 frames Cell Events: 4286 mean_circ: 0.950535
Ran for 87050 frames Cell Events: 4339 mean_circ: 0.950548
Ran for 87550 frames Cell Events: 4359 mean_circ: 0.95058
Ran for 89050 frames Cell Events: 4425 mean_circ: 0.950582
Ran for 90050 frames Cell Events: 4471 mean_circ: 0.950673
Ran for 91050 frames Cell Events: 4516 mean_circ: 0.950575
Ran for 91550 frames Cell Events: 4538 mean_circ: 0.950513
Ran for 92050 frames Cell Events: 4560 mean_circ: 0.950516
Ran for 92550 frames Cell Events: 4577 mean_circ: 0.950511
Ran for 97550 frames Cell Events: 4916 mean_circ: 0.950215
Ran for 98050 frames Cell Events: 4936 mean_circ: 0.950267
Ran for 98550 frames Cell Events: 4959 mean_circ: 0.950257
Ran for 99050 frames Cell Events: 4991 mean_circ: 0.95026
Ran for 99550 frames Cell Events: 5013 mean_circ: 0.950318
Ran for 100050 frames Cell Events: 5041 mean_circ: 0.950294
Ran for 101050 frames Cell Events: 5097 mean_circ: 0.950309
Ran for 101550 frames Cell Events: 5123 mean_circ: 0.950294
Ran for 103050 frames Cell Events: 5227 mean_circ: 0.950213
Ran for 105050 frames Cell Events: 5361 mean_circ: 0.950209
Ran for 105550 frames Cell Events: 5389 mean_circ: 0.950248
Ran for 106550 frames Cell Events: 5441 mean_circ: 0.950279
Ran for 107550 frames Cell Events: 5490 mean_circ: 0.950249
Ran for 108050 frames Cell Events: 5518 mean_circ: 0.950244
Ran for 108550 frames Cell Events: 5538 mean_circ: 0.950254
Ran for 109050 frames Cell Events: 5557 mean_circ: 0.950271
Ran for 109550 frames Cell Events: 5590 mean_circ: 0.950218
Ran for 110050 frames Cell Events: 5619 mean_circ: 0.9502
Ran for 111050 frames Cell Events: 5650 mean_circ: 0.950239
Ran for 111550 frames Cell Events: 5670 mean_circ: 0.950221
Ran for 112050 frames Cell Events: 5683 mean_circ: 0.950251
Ran for 112550 frames Cell Events: 5707 mean_circ: 0.950303
Ran for 114550 frames Cell Events: 5811 mean_circ: 0.950246
Ran for 115050 frames Cell Events: 5832 mean_circ: 0.950306
Ran for 115550 frames Cell Events: 5854 mean_circ: 0.950291
Ran for 116550 frames Cell Events: 5930 mean_circ: 0.950329
Ran for 117550 frames Cell Events: 5989 mean_circ: 0.950291
Ran for 118550 frames Cell Events: 6024 mean_circ: 0.950312
Ran for 119550 frames Cell Events: 6076 mean_circ: 0.950334
Ran for 120050 frames Cell Events: 6103 mean_circ: 0.950377
Ran for 120550 frames Cell Events: 6128 mean_circ: 0.950423
Ran for 123050 frames Cell Events: 6261 mean_circ: 0.950404
Ran for 123550 frames Cell Events: 6299 mean_circ: 0.950489
Ran for 124050 frames Cell Events: 6323 mean_circ: 0.950474
Ran for 124550 frames Cell Events: 6345 mean_circ: 0.95049
Ran for 126550 frames Cell Events: 6439 mean_circ: 0.950499
Ran for 127050 frames Cell Events: 6456 mean_circ: 0.950485
Ran for 129050 frames Cell Events: 6544 mean_circ: 0.950568
Ran for 130550 frames Cell Events: 6617 mean_circ: 0.950615
Ran for 132050 frames Cell Events: 6703 mean_circ: 0.950614
Ran for 133050 frames Cell Events: 6764 mean_circ: 0.950614
Ran for 135550 frames Cell Events: 6913 mean_circ: 0.950527
Ran for 136050 frames Cell Events: 6933 mean_circ: 0.950493
Ran for 137550 frames Cell Events: 7022 mean_circ: 0.950486
Ran for 139050 frames Cell Events: 7111 mean_circ: 0.950495
Ran for 140050 frames Cell Events: 7176 mean_circ: 0.950479
Ran for 141050 frames Cell Events: 7214 mean_circ: 0.950527
Ran for 141550 frames Cell Events: 7255 mean_circ: 0.950504
Ran for 142550 frames Cell Events: 7326 mean_circ: 0.95053
Ran for 143550 frames Cell Events: 7386 mean_circ: 0.950508
Ran for 145050 frames Cell Events: 7484 mean_circ: 0.950606
Ran for 146050 frames Cell Events: 7552 mean_circ: 0.950682
Ran for 147050 frames Cell Events: 7615 mean_circ: 0.950685
Ran for 148050 frames Cell Events: 7660 mean_circ: 0.950701
Ran for 148550 frames Cell Events: 7683 mean_circ: 0.950695
Ran for 149050 frames Cell Events: 7704 mean_circ: 0.950723
Ran for 151050 frames Cell Events: 7818 mean_circ: 0.950714
Ran for 151550 frames Cell Events: 7853 mean_circ: 0.95073
Ran for 152050 frames Cell Events: 7874 mean_circ: 0.950741
Ran for 155550 frames Cell Events: 8092 mean_circ: 0.950768
Ran for 157050 frames Cell Events: 8198 mean_circ: 0.950702
Ran for 160550 frames Cell Events: 8420 mean_circ: 0.950747
Ran for 163550 frames Cell Events: 8606 mean_circ: 0.950759
Ran for 164050 frames Cell Events: 8643 mean_circ: 0.950715



End this measurment..



Stopping time: 4.71569e+008
****************************************
Measurement finished

Mean Circularitiy: 0.950703
Mean Area: 601.185
Mean FL-1 intensity: 3538.15
Mean FL-2 intensity: 3294.87
Mean FL-3 intensity: 0

frames processed: 163141
Total Time: 82133.3ms
Number of Runs (new bg img) 0
Frames collected: 164079
Skipped frames: 5402
Rescued Frames: 824004
Samples DAQ: 41021641
Samples written: 82040202
Samples per Channel: 41020101
FPS: 1997.72

Samples per second: 499433
Expected samples/s: 500000

Events: 8644
Events per second: 105.244
jumped back (PMT): 4008
#Frames read, frequency
# 0 frames :254324
# 1 frames :59366
# 2 frames :3840
# 3 frames :1307
# 4 frames :757
# 5 frames :405
# 6 frames :1672
# 7 frames :1263
# 8 frames :889
# 9 frames :540
# 10 frames :780
# 11 frames :438
# 12 frames :376
# 13 frames :270
# 14 frames :264
# 15 frames :216
# 16 frames :209
# 17 frames :192
# 18 frames :142
# 19 frames :118
# 20 frames :78
# 21 frames :89
# 22 frames :66
# 23 frames :65
# 24 frames :46
# 25 frames :47
# 26 frames :33
# 27 frames :32
# 28 frames :25
# 29 frames :17
# 30 frames :12
# 31 frames :26
# 32 frames :15
# 33 frames :11
# 34 frames :10
# 35 frames :9
# 36 frames :8
# 37 frames :13
# 38 frames :11
# 39 frames :7
# 40 frames :7
# 41 frames :11
# 42 frames :1
# 43 frames :4
# 44 frames :2
# 45 frames :3
# 46 frames :2
# 47 frames :2
# 48 frames :1
# 49 frames :2
# 50 frames :2
# 51 frames :4
# 52 frames :1
# 54 frames :1
# 55 frames :3
# 56 frames :1
# 57 frames :1
# 59 frames :1
# 63 frames :1
# 71 frames :2
# 86 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.04
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 6
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 25.0721
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 6
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 300
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0