Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-10_17.57.23 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "5fad04abf2ac2a55a880ac654dd91758",
"name": "M1_20170615_1851_dsRNA_W2_col.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "15:57:44"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8128 (max 8129) in '8128'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 24.5884
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 1.42826e+008
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 1050 frames Cell Events: 58 mean_circ: 0.954888
Ran for 2050 frames Cell Events: 119 mean_circ: 0.955064
Ran for 2550 frames Cell Events: 140 mean_circ: 0.952451
Ran for 3050 frames Cell Events: 166 mean_circ: 0.952127
Ran for 3550 frames Cell Events: 186 mean_circ: 0.951618
Ran for 4050 frames Cell Events: 219 mean_circ: 0.951977
Ran for 5550 frames Cell Events: 312 mean_circ: 0.951689
Ran for 6050 frames Cell Events: 340 mean_circ: 0.951661
Ran for 6550 frames Cell Events: 373 mean_circ: 0.951723
Ran for 8050 frames Cell Events: 485 mean_circ: 0.952405
Ran for 9050 frames Cell Events: 537 mean_circ: 0.951543
Ran for 9550 frames Cell Events: 572 mean_circ: 0.951977
Ran for 10050 frames Cell Events: 609 mean_circ: 0.952314
Ran for 10550 frames Cell Events: 639 mean_circ: 0.952906
Ran for 11050 frames Cell Events: 674 mean_circ: 0.952977
Ran for 11550 frames Cell Events: 706 mean_circ: 0.952949
Ran for 12050 frames Cell Events: 736 mean_circ: 0.953685
Ran for 12550 frames Cell Events: 755 mean_circ: 0.953946
Ran for 14050 frames Cell Events: 807 mean_circ: 0.95434
Ran for 16550 frames Cell Events: 970 mean_circ: 0.953513
Ran for 17050 frames Cell Events: 1002 mean_circ: 0.953185
Ran for 17550 frames Cell Events: 1033 mean_circ: 0.953198
Ran for 19550 frames Cell Events: 1154 mean_circ: 0.952733
Ran for 21050 frames Cell Events: 1221 mean_circ: 0.952619
Ran for 22050 frames Cell Events: 1271 mean_circ: 0.952791
Ran for 23050 frames Cell Events: 1303 mean_circ: 0.952773
Ran for 23550 frames Cell Events: 1330 mean_circ: 0.952807
Ran for 24550 frames Cell Events: 1383 mean_circ: 0.952372
Ran for 25050 frames Cell Events: 1417 mean_circ: 0.952371
Ran for 26050 frames Cell Events: 1511 mean_circ: 0.952296
Ran for 26550 frames Cell Events: 1557 mean_circ: 0.952736
Ran for 27050 frames Cell Events: 1585 mean_circ: 0.952338
Ran for 28050 frames Cell Events: 1651 mean_circ: 0.952141
Ran for 28550 frames Cell Events: 1670 mean_circ: 0.952155
Ran for 29550 frames Cell Events: 1739 mean_circ: 0.952122
Ran for 30550 frames Cell Events: 1794 mean_circ: 0.952031
Ran for 31050 frames Cell Events: 1829 mean_circ: 0.952014
Ran for 32050 frames Cell Events: 1897 mean_circ: 0.951905
Ran for 33050 frames Cell Events: 1956 mean_circ: 0.951964
Ran for 33550 frames Cell Events: 1974 mean_circ: 0.951965
Ran for 34050 frames Cell Events: 1998 mean_circ: 0.95204
Ran for 37050 frames Cell Events: 2189 mean_circ: 0.951437
Ran for 38550 frames Cell Events: 2267 mean_circ: 0.951417
Ran for 40550 frames Cell Events: 2413 mean_circ: 0.951272
Ran for 41050 frames Cell Events: 2450 mean_circ: 0.95126
Ran for 41550 frames Cell Events: 2485 mean_circ: 0.951304
Ran for 42050 frames Cell Events: 2509 mean_circ: 0.951405
Ran for 43550 frames Cell Events: 2590 mean_circ: 0.951542
Ran for 44050 frames Cell Events: 2630 mean_circ: 0.951522
Ran for 45050 frames Cell Events: 2689 mean_circ: 0.951542
Ran for 46550 frames Cell Events: 2790 mean_circ: 0.951565
Ran for 47050 frames Cell Events: 2807 mean_circ: 0.951629
Ran for 49050 frames Cell Events: 2923 mean_circ: 0.951524
Ran for 49550 frames Cell Events: 2942 mean_circ: 0.951611
Ran for 50050 frames Cell Events: 2971 mean_circ: 0.951563
Ran for 50550 frames Cell Events: 3009 mean_circ: 0.951638
Ran for 51050 frames Cell Events: 3033 mean_circ: 0.951665
Ran for 52050 frames Cell Events: 3111 mean_circ: 0.951724
Ran for 52550 frames Cell Events: 3141 mean_circ: 0.951696
Ran for 54550 frames Cell Events: 3248 mean_circ: 0.951679
Ran for 55050 frames Cell Events: 3286 mean_circ: 0.951688
Ran for 56050 frames Cell Events: 3348 mean_circ: 0.951664
Ran for 59550 frames Cell Events: 3547 mean_circ: 0.951761
Ran for 60550 frames Cell Events: 3596 mean_circ: 0.951676
Ran for 61050 frames Cell Events: 3635 mean_circ: 0.951701
Ran for 61550 frames Cell Events: 3672 mean_circ: 0.951627
Ran for 62550 frames Cell Events: 3733 mean_circ: 0.951496
Ran for 63050 frames Cell Events: 3757 mean_circ: 0.951481
Ran for 65050 frames Cell Events: 3882 mean_circ: 0.951698
Ran for 66550 frames Cell Events: 3979 mean_circ: 0.95182
Ran for 67050 frames Cell Events: 4008 mean_circ: 0.951785
Ran for 68050 frames Cell Events: 4093 mean_circ: 0.951848
Ran for 68550 frames Cell Events: 4127 mean_circ: 0.951876
Ran for 70050 frames Cell Events: 4225 mean_circ: 0.951883
Ran for 71050 frames Cell Events: 4274 mean_circ: 0.951942
Ran for 71550 frames Cell Events: 4287 mean_circ: 0.951946
Ran for 72050 frames Cell Events: 4311 mean_circ: 0.951969
Ran for 72550 frames Cell Events: 4337 mean_circ: 0.951978
Ran for 73050 frames Cell Events: 4360 mean_circ: 0.951944
Ran for 74050 frames Cell Events: 4409 mean_circ: 0.951985
Ran for 75050 frames Cell Events: 4483 mean_circ: 0.951853
Ran for 75550 frames Cell Events: 4516 mean_circ: 0.951731
Ran for 76050 frames Cell Events: 4557 mean_circ: 0.951804
Ran for 76550 frames Cell Events: 4587 mean_circ: 0.951844
Ran for 77050 frames Cell Events: 4607 mean_circ: 0.951776
Ran for 78050 frames Cell Events: 4669 mean_circ: 0.951809
Ran for 78550 frames Cell Events: 4695 mean_circ: 0.951749
Ran for 79550 frames Cell Events: 4773 mean_circ: 0.951755
Ran for 80550 frames Cell Events: 4844 mean_circ: 0.951827
Ran for 81050 frames Cell Events: 4863 mean_circ: 0.951839
Ran for 81550 frames Cell Events: 4901 mean_circ: 0.951807
Ran for 82050 frames Cell Events: 4924 mean_circ: 0.951821
Ran for 82550 frames Cell Events: 4944 mean_circ: 0.951773
Ran for 83050 frames Cell Events: 4975 mean_circ: 0.951787
Ran for 84050 frames Cell Events: 5022 mean_circ: 0.951721
Ran for 84550 frames Cell Events: 5044 mean_circ: 0.951721
Ran for 85050 frames Cell Events: 5077 mean_circ: 0.951727
Ran for 86050 frames Cell Events: 5150 mean_circ: 0.951687
Ran for 86550 frames Cell Events: 5182 mean_circ: 0.951637
Ran for 87050 frames Cell Events: 5214 mean_circ: 0.951646
Ran for 88050 frames Cell Events: 5271 mean_circ: 0.951732
Ran for 90050 frames Cell Events: 5414 mean_circ: 0.951807
Ran for 91550 frames Cell Events: 5494 mean_circ: 0.951862
Ran for 92550 frames Cell Events: 5536 mean_circ: 0.951815
Ran for 93550 frames Cell Events: 5592 mean_circ: 0.95187
Ran for 94550 frames Cell Events: 5661 mean_circ: 0.951892
Ran for 95050 frames Cell Events: 5694 mean_circ: 0.951848
Ran for 95550 frames Cell Events: 5717 mean_circ: 0.951854
Ran for 96050 frames Cell Events: 5741 mean_circ: 0.951867
Ran for 96550 frames Cell Events: 5774 mean_circ: 0.951836
Ran for 97050 frames Cell Events: 5813 mean_circ: 0.951811
Ran for 99050 frames Cell Events: 5933 mean_circ: 0.951762
Ran for 100050 frames Cell Events: 6002 mean_circ: 0.951791
Ran for 101550 frames Cell Events: 6096 mean_circ: 0.951809
Ran for 102050 frames Cell Events: 6117 mean_circ: 0.951814
Ran for 102550 frames Cell Events: 6143 mean_circ: 0.951789
Ran for 103550 frames Cell Events: 6205 mean_circ: 0.95178
Ran for 104550 frames Cell Events: 6276 mean_circ: 0.951811
Ran for 105550 frames Cell Events: 6312 mean_circ: 0.951804
Ran for 106050 frames Cell Events: 6336 mean_circ: 0.951777
Ran for 106550 frames Cell Events: 6362 mean_circ: 0.951815
Ran for 108050 frames Cell Events: 6454 mean_circ: 0.951786
Ran for 108550 frames Cell Events: 6473 mean_circ: 0.951771
Ran for 110050 frames Cell Events: 6570 mean_circ: 0.951768
Ran for 110550 frames Cell Events: 6594 mean_circ: 0.951771
Ran for 111050 frames Cell Events: 6622 mean_circ: 0.951764
Ran for 111550 frames Cell Events: 6658 mean_circ: 0.951754
Ran for 112050 frames Cell Events: 6684 mean_circ: 0.951705
Ran for 112550 frames Cell Events: 6726 mean_circ: 0.951732
Ran for 113050 frames Cell Events: 6747 mean_circ: 0.951751
Ran for 114050 frames Cell Events: 6820 mean_circ: 0.951783
Ran for 114550 frames Cell Events: 6846 mean_circ: 0.951741
Ran for 115050 frames Cell Events: 6892 mean_circ: 0.951752
Ran for 115550 frames Cell Events: 6913 mean_circ: 0.951747
Ran for 117050 frames Cell Events: 6990 mean_circ: 0.951759
Ran for 117550 frames Cell Events: 7018 mean_circ: 0.951773
Ran for 118550 frames Cell Events: 7090 mean_circ: 0.951691
Ran for 119050 frames Cell Events: 7117 mean_circ: 0.951682
Ran for 119550 frames Cell Events: 7142 mean_circ: 0.951687
Ran for 121050 frames Cell Events: 7240 mean_circ: 0.951762
Ran for 122050 frames Cell Events: 7290 mean_circ: 0.951752
Ran for 123050 frames Cell Events: 7363 mean_circ: 0.951742
Ran for 123550 frames Cell Events: 7393 mean_circ: 0.951757
Ran for 125550 frames Cell Events: 7475 mean_circ: 0.951737
Ran for 126550 frames Cell Events: 7533 mean_circ: 0.951788
Ran for 127550 frames Cell Events: 7589 mean_circ: 0.951745
Ran for 128050 frames Cell Events: 7618 mean_circ: 0.951771
Ran for 128550 frames Cell Events: 7647 mean_circ: 0.951801
Ran for 129050 frames Cell Events: 7682 mean_circ: 0.951787
Ran for 129550 frames Cell Events: 7708 mean_circ: 0.95178
Ran for 130050 frames Cell Events: 7742 mean_circ: 0.951754
Ran for 131050 frames Cell Events: 7807 mean_circ: 0.951805
Ran for 131550 frames Cell Events: 7829 mean_circ: 0.951793
Ran for 132550 frames Cell Events: 7894 mean_circ: 0.951778
Ran for 133550 frames Cell Events: 7950 mean_circ: 0.951803
Ran for 134050 frames Cell Events: 7972 mean_circ: 0.951783
Ran for 134550 frames Cell Events: 8005 mean_circ: 0.95176
Ran for 135050 frames Cell Events: 8026 mean_circ: 0.951756
Ran for 135550 frames Cell Events: 8053 mean_circ: 0.951757
Ran for 136550 frames Cell Events: 8122 mean_circ: 0.951728



End this measurment..



Stopping time: 1.42894e+008
****************************************
Measurement finished

Mean Circularitiy: 0.951733
Mean Area: 624.789
Mean FL-1 intensity: 3507.15
Mean FL-2 intensity: 3353.67
Mean FL-3 intensity: 0

frames processed: 134228
Total Time: 68396.6ms
Number of Runs (new bg img) 0
Frames collected: 136594
Skipped frames: 19619
Rescued Frames: 1934818
Samples DAQ: 34151624
Samples written: 68297130
Samples per Channel: 34148565
FPS: 1997.09

Samples per second: 499273
Expected samples/s: 500000

Events: 8129
Events per second: 118.851
jumped back (PMT): 4486
#Frames read, frequency
# 0 frames :206216
# 1 frames :53992
# 2 frames :3379
# 3 frames :1259
# 4 frames :455
# 5 frames :2114
# 6 frames :1327
# 7 frames :922
# 8 frames :552
# 9 frames :375
# 10 frames :585
# 11 frames :427
# 12 frames :349
# 13 frames :186
# 14 frames :199
# 15 frames :116
# 16 frames :95
# 17 frames :98
# 18 frames :64
# 19 frames :65
# 20 frames :55
# 21 frames :35
# 22 frames :37
# 23 frames :22
# 24 frames :25
# 25 frames :24
# 26 frames :20
# 27 frames :14
# 28 frames :13
# 29 frames :10
# 30 frames :10
# 31 frames :1
# 32 frames :2
# 33 frames :2
# 34 frames :7
# 35 frames :1
# 36 frames :6
# 37 frames :2
# 38 frames :4
# 39 frames :4
# 40 frames :2
# 41 frames :4
# 42 frames :1
# 43 frames :1
# 45 frames :1
# 46 frames :1
# 47 frames :1
# 48 frames :1
# 49 frames :2
# 55 frames :2
# 64 frames :1
# 68 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.04
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 6
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 24.5884
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 6
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 300
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0