Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-10_19.34.09 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "a29d77a28e197596c934ff02b27c8c0c",
"name": "M1_20170620_1416_dsRNA_W2_col.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "17:34:32"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 9282 (max 9283) in '9282'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 24.5116
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 5.58344e+008
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 1050 frames Cell Events: 58 mean_circ: 0.950488
Ran for 2550 frames Cell Events: 155 mean_circ: 0.945962
Ran for 3050 frames Cell Events: 184 mean_circ: 0.946791
Ran for 4050 frames Cell Events: 249 mean_circ: 0.946429
Ran for 5550 frames Cell Events: 327 mean_circ: 0.947614
Ran for 6550 frames Cell Events: 378 mean_circ: 0.947586
Ran for 7550 frames Cell Events: 445 mean_circ: 0.946682
Ran for 8050 frames Cell Events: 482 mean_circ: 0.946808
Ran for 8550 frames Cell Events: 516 mean_circ: 0.946681
Ran for 9050 frames Cell Events: 564 mean_circ: 0.947442
Ran for 9550 frames Cell Events: 597 mean_circ: 0.947328
Ran for 12550 frames Cell Events: 767 mean_circ: 0.946994
Ran for 15050 frames Cell Events: 923 mean_circ: 0.947332
Ran for 16050 frames Cell Events: 967 mean_circ: 0.947254
Ran for 18550 frames Cell Events: 1151 mean_circ: 0.947699
Ran for 20550 frames Cell Events: 1287 mean_circ: 0.947343
Ran for 22050 frames Cell Events: 1369 mean_circ: 0.947396
Ran for 23050 frames Cell Events: 1425 mean_circ: 0.947436
Ran for 23550 frames Cell Events: 1468 mean_circ: 0.947436
Ran for 26050 frames Cell Events: 1597 mean_circ: 0.947661
Ran for 26550 frames Cell Events: 1627 mean_circ: 0.947474
Ran for 27050 frames Cell Events: 1644 mean_circ: 0.947641
Ran for 28550 frames Cell Events: 1736 mean_circ: 0.947812
Ran for 29050 frames Cell Events: 1767 mean_circ: 0.947863
Ran for 30550 frames Cell Events: 1859 mean_circ: 0.947458
Ran for 31550 frames Cell Events: 1909 mean_circ: 0.947417
Ran for 32550 frames Cell Events: 1970 mean_circ: 0.947322
Ran for 33550 frames Cell Events: 2028 mean_circ: 0.947354
Ran for 35550 frames Cell Events: 2131 mean_circ: 0.947149
Ran for 37050 frames Cell Events: 2234 mean_circ: 0.947507
Ran for 37550 frames Cell Events: 2264 mean_circ: 0.947584
Ran for 39550 frames Cell Events: 2383 mean_circ: 0.947444
Ran for 40050 frames Cell Events: 2416 mean_circ: 0.947436
Ran for 43050 frames Cell Events: 2612 mean_circ: 0.947146
Ran for 43550 frames Cell Events: 2631 mean_circ: 0.94721
Ran for 44550 frames Cell Events: 2694 mean_circ: 0.947308
Ran for 45550 frames Cell Events: 2765 mean_circ: 0.947266
Ran for 46050 frames Cell Events: 2789 mean_circ: 0.947262
Ran for 47050 frames Cell Events: 2847 mean_circ: 0.947332
Ran for 49550 frames Cell Events: 3012 mean_circ: 0.947126
Ran for 51550 frames Cell Events: 3179 mean_circ: 0.946999
Ran for 52050 frames Cell Events: 3215 mean_circ: 0.94714
Ran for 52550 frames Cell Events: 3242 mean_circ: 0.947148
Ran for 53050 frames Cell Events: 3274 mean_circ: 0.947201
Ran for 53550 frames Cell Events: 3297 mean_circ: 0.947239
Ran for 54050 frames Cell Events: 3333 mean_circ: 0.947239
Ran for 55050 frames Cell Events: 3423 mean_circ: 0.947101
Ran for 56050 frames Cell Events: 3484 mean_circ: 0.947017
Ran for 56550 frames Cell Events: 3516 mean_circ: 0.946937
Ran for 58050 frames Cell Events: 3598 mean_circ: 0.946967
Ran for 58550 frames Cell Events: 3627 mean_circ: 0.947065
Ran for 59050 frames Cell Events: 3662 mean_circ: 0.947162
Ran for 61550 frames Cell Events: 3790 mean_circ: 0.947429
Ran for 63550 frames Cell Events: 3936 mean_circ: 0.947196
Ran for 65050 frames Cell Events: 4010 mean_circ: 0.947182
Ran for 69050 frames Cell Events: 4258 mean_circ: 0.947259
Ran for 70050 frames Cell Events: 4317 mean_circ: 0.94717
Ran for 70550 frames Cell Events: 4336 mean_circ: 0.947186
Ran for 71050 frames Cell Events: 4361 mean_circ: 0.947226
Ran for 72550 frames Cell Events: 4454 mean_circ: 0.947164
Ran for 73050 frames Cell Events: 4491 mean_circ: 0.947162
Ran for 73550 frames Cell Events: 4525 mean_circ: 0.947207
Ran for 74050 frames Cell Events: 4556 mean_circ: 0.947242
Ran for 74550 frames Cell Events: 4582 mean_circ: 0.947292
Ran for 75550 frames Cell Events: 4646 mean_circ: 0.947281
Ran for 76550 frames Cell Events: 4721 mean_circ: 0.947229
Ran for 77050 frames Cell Events: 4773 mean_circ: 0.94713
Ran for 79550 frames Cell Events: 4938 mean_circ: 0.947165
Ran for 80050 frames Cell Events: 4973 mean_circ: 0.947006
Ran for 81050 frames Cell Events: 5041 mean_circ: 0.94698
Ran for 86550 frames Cell Events: 5418 mean_circ: 0.946991
Ran for 88550 frames Cell Events: 5544 mean_circ: 0.947016
Ran for 89050 frames Cell Events: 5575 mean_circ: 0.946993
Ran for 90050 frames Cell Events: 5615 mean_circ: 0.947009
Ran for 90550 frames Cell Events: 5640 mean_circ: 0.946979
Ran for 92050 frames Cell Events: 5728 mean_circ: 0.94692
Ran for 94550 frames Cell Events: 5878 mean_circ: 0.94686
Ran for 95050 frames Cell Events: 5890 mean_circ: 0.946872
Ran for 96550 frames Cell Events: 5984 mean_circ: 0.946919
Ran for 97050 frames Cell Events: 6021 mean_circ: 0.94692
Ran for 97550 frames Cell Events: 6048 mean_circ: 0.94694
Ran for 100050 frames Cell Events: 6166 mean_circ: 0.946905
Ran for 100550 frames Cell Events: 6191 mean_circ: 0.946902
Ran for 101050 frames Cell Events: 6218 mean_circ: 0.946908
Ran for 101550 frames Cell Events: 6244 mean_circ: 0.946899
Ran for 102050 frames Cell Events: 6265 mean_circ: 0.946918
Ran for 102550 frames Cell Events: 6294 mean_circ: 0.946875
Ran for 103550 frames Cell Events: 6360 mean_circ: 0.94685
Ran for 104550 frames Cell Events: 6415 mean_circ: 0.946874
Ran for 105050 frames Cell Events: 6449 mean_circ: 0.94688
Ran for 105550 frames Cell Events: 6470 mean_circ: 0.946866
Ran for 106050 frames Cell Events: 6490 mean_circ: 0.946885
Ran for 107550 frames Cell Events: 6593 mean_circ: 0.946826
Ran for 108050 frames Cell Events: 6630 mean_circ: 0.946848
Ran for 109550 frames Cell Events: 6731 mean_circ: 0.946728
Ran for 111550 frames Cell Events: 6893 mean_circ: 0.946688
Ran for 112550 frames Cell Events: 6978 mean_circ: 0.946587
Ran for 115050 frames Cell Events: 7131 mean_circ: 0.946533
Ran for 117550 frames Cell Events: 7306 mean_circ: 0.946491
Ran for 119550 frames Cell Events: 7422 mean_circ: 0.946328
Ran for 120050 frames Cell Events: 7453 mean_circ: 0.946328
Ran for 120550 frames Cell Events: 7491 mean_circ: 0.946308
Ran for 122050 frames Cell Events: 7586 mean_circ: 0.946253
Ran for 123050 frames Cell Events: 7664 mean_circ: 0.946204
Ran for 123550 frames Cell Events: 7690 mean_circ: 0.94623
Ran for 124050 frames Cell Events: 7714 mean_circ: 0.946234
Ran for 124550 frames Cell Events: 7747 mean_circ: 0.946218
Ran for 126050 frames Cell Events: 7852 mean_circ: 0.946105
Ran for 127550 frames Cell Events: 7964 mean_circ: 0.946067
Ran for 132050 frames Cell Events: 8250 mean_circ: 0.946079
Ran for 132550 frames Cell Events: 8265 mean_circ: 0.946124
Ran for 133550 frames Cell Events: 8337 mean_circ: 0.946155
Ran for 135050 frames Cell Events: 8429 mean_circ: 0.946153
Ran for 137050 frames Cell Events: 8564 mean_circ: 0.946103
Ran for 137550 frames Cell Events: 8586 mean_circ: 0.946086
Ran for 139550 frames Cell Events: 8682 mean_circ: 0.94609
Ran for 140050 frames Cell Events: 8703 mean_circ: 0.946043
Ran for 140550 frames Cell Events: 8731 mean_circ: 0.946039
Ran for 141550 frames Cell Events: 8819 mean_circ: 0.946051
Ran for 142550 frames Cell Events: 8887 mean_circ: 0.946026
Ran for 143050 frames Cell Events: 8927 mean_circ: 0.945993
Ran for 144050 frames Cell Events: 8999 mean_circ: 0.945958
Ran for 144550 frames Cell Events: 9025 mean_circ: 0.945954
Ran for 146550 frames Cell Events: 9188 mean_circ: 0.94587
Ran for 147050 frames Cell Events: 9207 mean_circ: 0.945859



End this measurment..



Stopping time: 5.58418e+008
****************************************
Measurement finished

Mean Circularitiy: 0.94585
Mean Area: 637.336
Mean FL-1 intensity: 3939.03
Mean FL-2 intensity: 3111.13
Mean FL-3 intensity: 0

frames processed: 146879
Total Time: 74280.1ms
Number of Runs (new bg img) 0
Frames collected: 148383
Skipped frames: 4028
Rescued Frames: 943711
Samples DAQ: 37095664
Samples written: 74191280
Samples per Channel: 37095640
FPS: 1997.62

Samples per second: 499402
Expected samples/s: 500000

Events: 9283
Events per second: 124.973
jumped back (PMT): 4103
#Frames read, frequency
# 0 frames :236967
# 1 frames :46760
# 2 frames :3039
# 3 frames :1113
# 4 frames :623
# 5 frames :384
# 6 frames :1837
# 7 frames :1137
# 8 frames :852
# 9 frames :522
# 10 frames :694
# 11 frames :397
# 12 frames :327
# 13 frames :231
# 14 frames :258
# 15 frames :240
# 16 frames :206
# 17 frames :164
# 18 frames :106
# 19 frames :106
# 20 frames :79
# 21 frames :94
# 22 frames :72
# 23 frames :64
# 24 frames :58
# 25 frames :51
# 26 frames :35
# 27 frames :32
# 28 frames :22
# 29 frames :23
# 30 frames :20
# 31 frames :22
# 32 frames :17
# 33 frames :20
# 34 frames :14
# 35 frames :19
# 36 frames :13
# 37 frames :15
# 38 frames :13
# 39 frames :11
# 40 frames :11
# 41 frames :6
# 42 frames :8
# 43 frames :6
# 44 frames :8
# 45 frames :4
# 46 frames :4
# 47 frames :2
# 48 frames :1
# 49 frames :2
# 50 frames :4
# 51 frames :4
# 52 frames :3
# 53 frames :2
# 54 frames :3
# 55 frames :1
# 56 frames :1
# 57 frames :1
# 58 frames :2
# 60 frames :1
# 62 frames :2
# 63 frames :1
# 64 frames :1
# 65 frames :1
# 66 frames :1
# 69 frames :1
# 81 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.04
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 6
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 24.5116
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 6
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 300
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0