Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-10_17.32.25 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "0ffe1d1b52ba69bf5e7c244adc6eac45",
"name": "M1_20170613_1923_dsRNA_Y1_col.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "15:32:47"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8389 (max 8390) in '8389'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 24.6812
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 2.54523e+009
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 550 frames Cell Events: 26 mean_circ: 0.94487
Ran for 2050 frames Cell Events: 78 mean_circ: 0.937463
Ran for 2550 frames Cell Events: 99 mean_circ: 0.938195
Ran for 3550 frames Cell Events: 145 mean_circ: 0.936604
Ran for 5050 frames Cell Events: 217 mean_circ: 0.936365
Ran for 5550 frames Cell Events: 235 mean_circ: 0.936724
Ran for 6050 frames Cell Events: 264 mean_circ: 0.937235
Ran for 6550 frames Cell Events: 295 mean_circ: 0.936296
Ran for 7050 frames Cell Events: 322 mean_circ: 0.935869
Ran for 7550 frames Cell Events: 356 mean_circ: 0.935924
Ran for 8050 frames Cell Events: 378 mean_circ: 0.936682
Ran for 9550 frames Cell Events: 450 mean_circ: 0.93784
Ran for 10050 frames Cell Events: 480 mean_circ: 0.937982
Ran for 11050 frames Cell Events: 548 mean_circ: 0.934319
Ran for 12050 frames Cell Events: 585 mean_circ: 0.934832
Ran for 12550 frames Cell Events: 609 mean_circ: 0.935244
Ran for 13050 frames Cell Events: 628 mean_circ: 0.935445
Ran for 14050 frames Cell Events: 696 mean_circ: 0.935168
Ran for 14550 frames Cell Events: 725 mean_circ: 0.935104
Ran for 19050 frames Cell Events: 991 mean_circ: 0.934541
Ran for 20050 frames Cell Events: 1039 mean_circ: 0.934896
Ran for 20550 frames Cell Events: 1061 mean_circ: 0.935006
Ran for 21050 frames Cell Events: 1081 mean_circ: 0.934994
Ran for 21550 frames Cell Events: 1096 mean_circ: 0.935144
Ran for 22550 frames Cell Events: 1158 mean_circ: 0.935068
Ran for 23550 frames Cell Events: 1204 mean_circ: 0.93556
Ran for 24550 frames Cell Events: 1264 mean_circ: 0.935611
Ran for 25050 frames Cell Events: 1281 mean_circ: 0.935712
Ran for 25550 frames Cell Events: 1308 mean_circ: 0.935979
Ran for 26050 frames Cell Events: 1329 mean_circ: 0.935965
Ran for 26550 frames Cell Events: 1355 mean_circ: 0.935851
Ran for 27050 frames Cell Events: 1388 mean_circ: 0.936154
Ran for 27550 frames Cell Events: 1406 mean_circ: 0.936213
Ran for 28550 frames Cell Events: 1448 mean_circ: 0.936338
Ran for 29050 frames Cell Events: 1471 mean_circ: 0.936117
Ran for 31050 frames Cell Events: 1565 mean_circ: 0.936176
Ran for 32550 frames Cell Events: 1619 mean_circ: 0.936323
Ran for 33050 frames Cell Events: 1640 mean_circ: 0.936159
Ran for 33550 frames Cell Events: 1658 mean_circ: 0.936041
Ran for 34050 frames Cell Events: 1689 mean_circ: 0.935418
Ran for 34550 frames Cell Events: 1716 mean_circ: 0.935452
Ran for 35050 frames Cell Events: 1740 mean_circ: 0.935606
Ran for 36050 frames Cell Events: 1781 mean_circ: 0.935708
Ran for 37050 frames Cell Events: 1829 mean_circ: 0.935894
Ran for 38050 frames Cell Events: 1854 mean_circ: 0.936024
Ran for 38550 frames Cell Events: 1870 mean_circ: 0.936279
Ran for 39050 frames Cell Events: 1882 mean_circ: 0.936426
Ran for 39550 frames Cell Events: 1892 mean_circ: 0.936426
Ran for 40050 frames Cell Events: 1918 mean_circ: 0.936221
Ran for 40550 frames Cell Events: 1952 mean_circ: 0.936403
Ran for 41050 frames Cell Events: 1981 mean_circ: 0.936588
Ran for 43050 frames Cell Events: 2076 mean_circ: 0.936458
Ran for 44550 frames Cell Events: 2172 mean_circ: 0.936835
Ran for 45050 frames Cell Events: 2203 mean_circ: 0.936762
Ran for 45550 frames Cell Events: 2219 mean_circ: 0.936874
Ran for 46550 frames Cell Events: 2280 mean_circ: 0.936824
Ran for 48550 frames Cell Events: 2410 mean_circ: 0.937191
Ran for 49050 frames Cell Events: 2438 mean_circ: 0.937264
Ran for 50550 frames Cell Events: 2542 mean_circ: 0.937172
Ran for 51550 frames Cell Events: 2607 mean_circ: 0.9374
Ran for 52050 frames Cell Events: 2640 mean_circ: 0.937273
Ran for 53050 frames Cell Events: 2693 mean_circ: 0.937465
Ran for 54550 frames Cell Events: 2766 mean_circ: 0.937623
Ran for 55050 frames Cell Events: 2793 mean_circ: 0.93743
Ran for 55550 frames Cell Events: 2804 mean_circ: 0.937522
Ran for 56050 frames Cell Events: 2820 mean_circ: 0.937584
Ran for 56550 frames Cell Events: 2840 mean_circ: 0.937571
Ran for 57050 frames Cell Events: 2859 mean_circ: 0.937601
Ran for 57550 frames Cell Events: 2881 mean_circ: 0.937632
Ran for 58050 frames Cell Events: 2908 mean_circ: 0.937619
Ran for 58550 frames Cell Events: 2937 mean_circ: 0.937614
Ran for 59050 frames Cell Events: 2964 mean_circ: 0.937609
Ran for 61050 frames Cell Events: 3072 mean_circ: 0.937638
Ran for 61550 frames Cell Events: 3095 mean_circ: 0.937755
Ran for 62050 frames Cell Events: 3120 mean_circ: 0.937778
Ran for 63050 frames Cell Events: 3179 mean_circ: 0.937655
Ran for 63550 frames Cell Events: 3218 mean_circ: 0.937637
Ran for 65050 frames Cell Events: 3316 mean_circ: 0.937644
Ran for 66550 frames Cell Events: 3380 mean_circ: 0.937637
Ran for 67050 frames Cell Events: 3401 mean_circ: 0.937718
Ran for 69050 frames Cell Events: 3504 mean_circ: 0.937648
Ran for 71550 frames Cell Events: 3652 mean_circ: 0.937576
Ran for 72050 frames Cell Events: 3671 mean_circ: 0.937573
Ran for 72550 frames Cell Events: 3696 mean_circ: 0.937583
Ran for 73050 frames Cell Events: 3715 mean_circ: 0.937447
Ran for 76050 frames Cell Events: 3892 mean_circ: 0.937072
Ran for 77050 frames Cell Events: 3951 mean_circ: 0.937157
Ran for 77550 frames Cell Events: 3978 mean_circ: 0.937223
Ran for 79050 frames Cell Events: 4062 mean_circ: 0.937225
Ran for 80550 frames Cell Events: 4128 mean_circ: 0.937147
Ran for 81050 frames Cell Events: 4159 mean_circ: 0.937189
Ran for 83050 frames Cell Events: 4295 mean_circ: 0.93706
Ran for 84550 frames Cell Events: 4372 mean_circ: 0.937215
Ran for 85050 frames Cell Events: 4386 mean_circ: 0.937214
Ran for 85550 frames Cell Events: 4396 mean_circ: 0.937249
Ran for 86050 frames Cell Events: 4410 mean_circ: 0.937238
Ran for 87050 frames Cell Events: 4471 mean_circ: 0.937254
Ran for 87550 frames Cell Events: 4504 mean_circ: 0.937312
Ran for 90050 frames Cell Events: 4642 mean_circ: 0.937333
Ran for 90550 frames Cell Events: 4676 mean_circ: 0.937382
Ran for 91550 frames Cell Events: 4758 mean_circ: 0.937365
Ran for 92050 frames Cell Events: 4800 mean_circ: 0.937445
Ran for 95050 frames Cell Events: 4953 mean_circ: 0.937604
Ran for 97550 frames Cell Events: 5082 mean_circ: 0.937668
Ran for 98050 frames Cell Events: 5113 mean_circ: 0.937627
Ran for 98550 frames Cell Events: 5140 mean_circ: 0.937554
Ran for 99050 frames Cell Events: 5179 mean_circ: 0.937554
Ran for 100050 frames Cell Events: 5249 mean_circ: 0.937488
Ran for 101050 frames Cell Events: 5305 mean_circ: 0.937535
Ran for 103050 frames Cell Events: 5404 mean_circ: 0.937477
Ran for 105050 frames Cell Events: 5523 mean_circ: 0.93749
Ran for 105550 frames Cell Events: 5556 mean_circ: 0.937467
Ran for 108050 frames Cell Events: 5674 mean_circ: 0.937467
Ran for 108550 frames Cell Events: 5699 mean_circ: 0.937471
Ran for 109550 frames Cell Events: 5763 mean_circ: 0.937603
Ran for 110550 frames Cell Events: 5817 mean_circ: 0.937643
Ran for 112050 frames Cell Events: 5916 mean_circ: 0.937674
Ran for 112550 frames Cell Events: 5946 mean_circ: 0.937694
Ran for 115550 frames Cell Events: 6150 mean_circ: 0.937424
Ran for 117050 frames Cell Events: 6254 mean_circ: 0.937315
Ran for 118050 frames Cell Events: 6320 mean_circ: 0.937312
Ran for 119550 frames Cell Events: 6414 mean_circ: 0.937359
Ran for 120550 frames Cell Events: 6462 mean_circ: 0.937485
Ran for 121550 frames Cell Events: 6520 mean_circ: 0.937516
Ran for 123050 frames Cell Events: 6587 mean_circ: 0.937516
Ran for 124550 frames Cell Events: 6679 mean_circ: 0.937457
Ran for 126050 frames Cell Events: 6773 mean_circ: 0.937519
Ran for 127050 frames Cell Events: 6824 mean_circ: 0.9375
Ran for 129550 frames Cell Events: 6959 mean_circ: 0.937453
Ran for 130050 frames Cell Events: 7000 mean_circ: 0.937477
Ran for 132050 frames Cell Events: 7116 mean_circ: 0.937435
Ran for 135550 frames Cell Events: 7311 mean_circ: 0.937449
Ran for 136550 frames Cell Events: 7374 mean_circ: 0.9375
Ran for 137050 frames Cell Events: 7401 mean_circ: 0.93747
Ran for 138050 frames Cell Events: 7471 mean_circ: 0.937494
Ran for 138550 frames Cell Events: 7497 mean_circ: 0.937494
Ran for 139050 frames Cell Events: 7520 mean_circ: 0.937561
Ran for 140550 frames Cell Events: 7607 mean_circ: 0.93759
Ran for 141050 frames Cell Events: 7624 mean_circ: 0.937543
Ran for 142050 frames Cell Events: 7685 mean_circ: 0.937599
Ran for 143550 frames Cell Events: 7786 mean_circ: 0.937452
Ran for 144050 frames Cell Events: 7814 mean_circ: 0.937537
Ran for 145050 frames Cell Events: 7860 mean_circ: 0.937589
Ran for 146050 frames Cell Events: 7906 mean_circ: 0.937595
Ran for 146550 frames Cell Events: 7924 mean_circ: 0.937609
Ran for 147050 frames Cell Events: 7948 mean_circ: 0.937619
Ran for 147550 frames Cell Events: 7968 mean_circ: 0.937614
Ran for 148550 frames Cell Events: 8026 mean_circ: 0.937604
Ran for 150050 frames Cell Events: 8106 mean_circ: 0.937579
Ran for 151050 frames Cell Events: 8158 mean_circ: 0.93763
Ran for 152050 frames Cell Events: 8196 mean_circ: 0.937588
Ran for 152550 frames Cell Events: 8224 mean_circ: 0.937603
Ran for 154050 frames Cell Events: 8311 mean_circ: 0.937689
Ran for 155050 frames Cell Events: 8370 mean_circ: 0.937661



End this measurment..



Stopping time: 2.54531e+009
****************************************
Measurement finished

Mean Circularitiy: 0.937677
Mean Area: 649.495
Mean FL-1 intensity: 3179.89
Mean FL-2 intensity: 3316.15
Mean FL-3 intensity: 0

frames processed: 154145
Total Time: 77806.7ms
Number of Runs (new bg img) 0
Frames collected: 155474
Skipped frames: 78680
Rescued Frames: 5930360
Samples DAQ: 38868558
Samples written: 77736786
Samples per Channel: 38868393
FPS: 1998.21

Samples per second: 499551
Expected samples/s: 500000

Events: 8390
Events per second: 107.831
jumped back (PMT): 4239
#Frames read, frequency
# 0 frames :242365
# 1 frames :54480
# 2 frames :3978
# 3 frames :1184
# 4 frames :802
# 5 frames :397
# 6 frames :1026
# 7 frames :1791
# 8 frames :989
# 9 frames :680
# 10 frames :663
# 11 frames :434
# 12 frames :294
# 13 frames :233
# 14 frames :194
# 15 frames :209
# 16 frames :197
# 17 frames :153
# 18 frames :118
# 19 frames :83
# 20 frames :74
# 21 frames :82
# 22 frames :67
# 23 frames :50
# 24 frames :43
# 25 frames :38
# 26 frames :34
# 27 frames :35
# 28 frames :28
# 29 frames :27
# 30 frames :19
# 31 frames :15
# 32 frames :19
# 33 frames :12
# 34 frames :8
# 35 frames :10
# 36 frames :13
# 37 frames :15
# 38 frames :4
# 39 frames :3
# 40 frames :6
# 41 frames :6
# 42 frames :4
# 43 frames :4
# 44 frames :2
# 45 frames :2
# 46 frames :7
# 47 frames :1
# 48 frames :1
# 49 frames :5
# 50 frames :3
# 51 frames :3
# 52 frames :3
# 53 frames :1
# 54 frames :2
# 55 frames :1
# 56 frames :2
# 58 frames :1
# 59 frames :1
# 60 frames :2
# 61 frames :2
# 62 frames :1
# 63 frames :2
# 71 frames :1
# 74 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.04
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 6
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 24.6812
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 6
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 300
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0