Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-10_17.44.52 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "7d2ca12f21b952ca4c3f37050c008532",
"name": "M1_20170615_1531_dsRNA_Z1_col.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "15:45:13"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8212 (max 8213) in '8212'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 24.6908
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 1.30828e+008
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 2050 frames Cell Events: 153 mean_circ: 0.949368
Ran for 2550 frames Cell Events: 196 mean_circ: 0.949564
Ran for 4550 frames Cell Events: 354 mean_circ: 0.949998
Ran for 6550 frames Cell Events: 525 mean_circ: 0.947499
Ran for 7050 frames Cell Events: 557 mean_circ: 0.946795
Ran for 8050 frames Cell Events: 639 mean_circ: 0.946555
Ran for 10550 frames Cell Events: 807 mean_circ: 0.947413
Ran for 11550 frames Cell Events: 865 mean_circ: 0.947945
Ran for 12550 frames Cell Events: 933 mean_circ: 0.948173
Ran for 13550 frames Cell Events: 1001 mean_circ: 0.948146
Ran for 16050 frames Cell Events: 1201 mean_circ: 0.947467
Ran for 17050 frames Cell Events: 1261 mean_circ: 0.948045
Ran for 19050 frames Cell Events: 1405 mean_circ: 0.947737
Ran for 20550 frames Cell Events: 1498 mean_circ: 0.947434
Ran for 21050 frames Cell Events: 1524 mean_circ: 0.947351
Ran for 22050 frames Cell Events: 1598 mean_circ: 0.947285
Ran for 25050 frames Cell Events: 1779 mean_circ: 0.947215
Ran for 25550 frames Cell Events: 1808 mean_circ: 0.947253
Ran for 26550 frames Cell Events: 1875 mean_circ: 0.947242
Ran for 28050 frames Cell Events: 1964 mean_circ: 0.94731
Ran for 29050 frames Cell Events: 2038 mean_circ: 0.947286
Ran for 30050 frames Cell Events: 2107 mean_circ: 0.947122
Ran for 30550 frames Cell Events: 2143 mean_circ: 0.94719
Ran for 32050 frames Cell Events: 2246 mean_circ: 0.946767
Ran for 33050 frames Cell Events: 2313 mean_circ: 0.946609
Ran for 34050 frames Cell Events: 2380 mean_circ: 0.946641
Ran for 35050 frames Cell Events: 2445 mean_circ: 0.946656
Ran for 36550 frames Cell Events: 2542 mean_circ: 0.946797
Ran for 38050 frames Cell Events: 2651 mean_circ: 0.946529
Ran for 39050 frames Cell Events: 2727 mean_circ: 0.94682
Ran for 40550 frames Cell Events: 2858 mean_circ: 0.946696
Ran for 44550 frames Cell Events: 3188 mean_circ: 0.946682
Ran for 45550 frames Cell Events: 3260 mean_circ: 0.946779
Ran for 46050 frames Cell Events: 3301 mean_circ: 0.94678
Ran for 48050 frames Cell Events: 3423 mean_circ: 0.946653
Ran for 49050 frames Cell Events: 3497 mean_circ: 0.946599
Ran for 50050 frames Cell Events: 3556 mean_circ: 0.946497
Ran for 50550 frames Cell Events: 3594 mean_circ: 0.946426
Ran for 51050 frames Cell Events: 3621 mean_circ: 0.946458
Ran for 52050 frames Cell Events: 3709 mean_circ: 0.946487
Ran for 54550 frames Cell Events: 3893 mean_circ: 0.946468
Ran for 57550 frames Cell Events: 4115 mean_circ: 0.946573
Ran for 58050 frames Cell Events: 4155 mean_circ: 0.946563
Ran for 58550 frames Cell Events: 4180 mean_circ: 0.946575
Ran for 61550 frames Cell Events: 4415 mean_circ: 0.946667
Ran for 63050 frames Cell Events: 4519 mean_circ: 0.946777
Ran for 63550 frames Cell Events: 4554 mean_circ: 0.946769
Ran for 64050 frames Cell Events: 4584 mean_circ: 0.946709
Ran for 64550 frames Cell Events: 4614 mean_circ: 0.946712
Ran for 65050 frames Cell Events: 4642 mean_circ: 0.946767
Ran for 66550 frames Cell Events: 4721 mean_circ: 0.946746
Ran for 67050 frames Cell Events: 4744 mean_circ: 0.946881
Ran for 70550 frames Cell Events: 4985 mean_circ: 0.946888
Ran for 71050 frames Cell Events: 5014 mean_circ: 0.946964
Ran for 72050 frames Cell Events: 5083 mean_circ: 0.94697
Ran for 73050 frames Cell Events: 5156 mean_circ: 0.946932
Ran for 74550 frames Cell Events: 5258 mean_circ: 0.946843
Ran for 78050 frames Cell Events: 5510 mean_circ: 0.946781
Ran for 79050 frames Cell Events: 5581 mean_circ: 0.946757
Ran for 81550 frames Cell Events: 5748 mean_circ: 0.946719
Ran for 82550 frames Cell Events: 5833 mean_circ: 0.946663
Ran for 84550 frames Cell Events: 6007 mean_circ: 0.946432
Ran for 85550 frames Cell Events: 6086 mean_circ: 0.946398
Ran for 86050 frames Cell Events: 6119 mean_circ: 0.946355
Ran for 88550 frames Cell Events: 6272 mean_circ: 0.946383
Ran for 89050 frames Cell Events: 6309 mean_circ: 0.946426
Ran for 90050 frames Cell Events: 6381 mean_circ: 0.946287
Ran for 97550 frames Cell Events: 6950 mean_circ: 0.946316
Ran for 98050 frames Cell Events: 6997 mean_circ: 0.94632
Ran for 99050 frames Cell Events: 7061 mean_circ: 0.946283
Ran for 101050 frames Cell Events: 7227 mean_circ: 0.94625
Ran for 101550 frames Cell Events: 7261 mean_circ: 0.946261
Ran for 102050 frames Cell Events: 7294 mean_circ: 0.946299
Ran for 102550 frames Cell Events: 7329 mean_circ: 0.946302
Ran for 106550 frames Cell Events: 7661 mean_circ: 0.946304
Ran for 107050 frames Cell Events: 7709 mean_circ: 0.946304
Ran for 108050 frames Cell Events: 7798 mean_circ: 0.946251
Ran for 109050 frames Cell Events: 7851 mean_circ: 0.946251
Ran for 109550 frames Cell Events: 7872 mean_circ: 0.946245
Ran for 110550 frames Cell Events: 7926 mean_circ: 0.946201
Ran for 112050 frames Cell Events: 8035 mean_circ: 0.94617
Ran for 114050 frames Cell Events: 8184 mean_circ: 0.946211



End this measurment..



Stopping time: 1.30886e+008
****************************************
Measurement finished

Mean Circularitiy: 0.946209
Mean Area: 626.05
Mean FL-1 intensity: 3352.27
Mean FL-2 intensity: 3000.55
Mean FL-3 intensity: 0

frames processed: 110244
Total Time: 57300.9ms
Number of Runs (new bg img) 0
Frames collected: 114476
Skipped frames: 14686
Rescued Frames: 1003125
Samples DAQ: 28619065
Samples written: 57238030
Samples per Channel: 28619015
FPS: 1997.8

Samples per second: 499451
Expected samples/s: 500000

Events: 8213
Events per second: 143.331
jumped back (PMT): 2692
#Frames read, frequency
# 0 frames :189912
# 1 frames :26529
# 2 frames :1924
# 3 frames :767
# 4 frames :417
# 5 frames :263
# 6 frames :1312
# 7 frames :656
# 8 frames :590
# 9 frames :375
# 10 frames :449
# 11 frames :330
# 12 frames :229
# 13 frames :174
# 14 frames :232
# 15 frames :167
# 16 frames :169
# 17 frames :146
# 18 frames :118
# 19 frames :94
# 20 frames :88
# 21 frames :90
# 22 frames :77
# 23 frames :76
# 24 frames :57
# 25 frames :53
# 26 frames :44
# 27 frames :50
# 28 frames :29
# 29 frames :24
# 30 frames :27
# 31 frames :29
# 32 frames :35
# 33 frames :25
# 34 frames :23
# 35 frames :14
# 36 frames :17
# 37 frames :28
# 38 frames :8
# 39 frames :17
# 40 frames :20
# 41 frames :17
# 42 frames :11
# 43 frames :11
# 44 frames :10
# 45 frames :8
# 46 frames :6
# 47 frames :9
# 48 frames :4
# 49 frames :6
# 50 frames :6
# 51 frames :6
# 52 frames :8
# 53 frames :1
# 54 frames :5
# 55 frames :3
# 56 frames :2
# 57 frames :2
# 58 frames :4
# 60 frames :2
# 61 frames :3
# 62 frames :3
# 63 frames :1
# 65 frames :2
# 66 frames :3
# 67 frames :1
# 69 frames :1
# 70 frames :1
# 72 frames :1
# 73 frames :1
# 76 frames :1
# 77 frames :1
# 81 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.04
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 6
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 24.6908
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 6
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 300
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0