Click on the log titles below to expand the log entries present in this DC resource.

dclab-export_2024-07-10_17.41.46 {"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc {
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "36a44a9d79af06454215c28c1d07a35a",
"name": "M1_20170615_1438_dsRNA_Z2_col.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "15:42:12"
}
}
dclab-tdms2rtdc-warnings LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 10001 (max 10002) in
'10001'.
FG_Config.mcf [Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;

[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;

[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini [Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6

[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini [EVENT LOG] fRT-DC V1.06170206

Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 24.6888
Start pulse train for BG Aquisition..

Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 1.27666e+008
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 1050 frames Cell Events: 29 mean_circ: 0.932321
Ran for 1550 frames Cell Events: 59 mean_circ: 0.928642
Ran for 4550 frames Cell Events: 272 mean_circ: 0.937308
Ran for 6050 frames Cell Events: 350 mean_circ: 0.93647
Ran for 6550 frames Cell Events: 375 mean_circ: 0.936915
Ran for 7550 frames Cell Events: 436 mean_circ: 0.935903
Ran for 8050 frames Cell Events: 463 mean_circ: 0.93582
Ran for 8550 frames Cell Events: 495 mean_circ: 0.935197
Ran for 10050 frames Cell Events: 594 mean_circ: 0.933161
Ran for 11050 frames Cell Events: 651 mean_circ: 0.933747
Ran for 13050 frames Cell Events: 777 mean_circ: 0.933379
Ran for 14050 frames Cell Events: 832 mean_circ: 0.933153
Ran for 15550 frames Cell Events: 901 mean_circ: 0.933355
Ran for 16550 frames Cell Events: 963 mean_circ: 0.933093
Ran for 17050 frames Cell Events: 990 mean_circ: 0.93331
Ran for 19550 frames Cell Events: 1200 mean_circ: 0.932674
Ran for 20050 frames Cell Events: 1221 mean_circ: 0.932454
Ran for 20550 frames Cell Events: 1254 mean_circ: 0.932293
Ran for 22550 frames Cell Events: 1357 mean_circ: 0.932189
Ran for 23550 frames Cell Events: 1399 mean_circ: 0.932293
Ran for 25050 frames Cell Events: 1495 mean_circ: 0.932316
Ran for 26550 frames Cell Events: 1575 mean_circ: 0.932317
Ran for 27550 frames Cell Events: 1635 mean_circ: 0.93233
Ran for 31050 frames Cell Events: 1831 mean_circ: 0.932317
Ran for 31550 frames Cell Events: 1865 mean_circ: 0.93226
Ran for 32550 frames Cell Events: 1916 mean_circ: 0.932198
Ran for 33550 frames Cell Events: 1991 mean_circ: 0.932061
Ran for 34050 frames Cell Events: 2025 mean_circ: 0.932027
Ran for 34550 frames Cell Events: 2049 mean_circ: 0.931723
Ran for 40050 frames Cell Events: 2338 mean_circ: 0.931297
Ran for 41050 frames Cell Events: 2402 mean_circ: 0.931267
Ran for 42050 frames Cell Events: 2462 mean_circ: 0.931068
Ran for 42550 frames Cell Events: 2491 mean_circ: 0.931169
Ran for 43050 frames Cell Events: 2508 mean_circ: 0.931055
Ran for 43550 frames Cell Events: 2534 mean_circ: 0.931093
Ran for 44550 frames Cell Events: 2568 mean_circ: 0.931018
Ran for 45050 frames Cell Events: 2603 mean_circ: 0.93108
Ran for 45550 frames Cell Events: 2638 mean_circ: 0.931031
Ran for 46050 frames Cell Events: 2662 mean_circ: 0.930899
Ran for 46550 frames Cell Events: 2698 mean_circ: 0.930769
Ran for 47550 frames Cell Events: 2749 mean_circ: 0.93075
Ran for 48550 frames Cell Events: 2806 mean_circ: 0.930709
Ran for 49050 frames Cell Events: 2830 mean_circ: 0.93064
Ran for 49550 frames Cell Events: 2855 mean_circ: 0.930619
Ran for 50050 frames Cell Events: 2868 mean_circ: 0.930475
Ran for 51550 frames Cell Events: 2958 mean_circ: 0.930278
Ran for 52550 frames Cell Events: 3033 mean_circ: 0.930169
Ran for 53050 frames Cell Events: 3064 mean_circ: 0.929952
Ran for 54050 frames Cell Events: 3114 mean_circ: 0.929973
Ran for 54550 frames Cell Events: 3137 mean_circ: 0.929991
Ran for 55050 frames Cell Events: 3172 mean_circ: 0.930094
Ran for 55550 frames Cell Events: 3191 mean_circ: 0.930083
Ran for 56550 frames Cell Events: 3253 mean_circ: 0.92997
Ran for 59050 frames Cell Events: 3403 mean_circ: 0.930159
Ran for 61050 frames Cell Events: 3520 mean_circ: 0.929936
Ran for 62550 frames Cell Events: 3634 mean_circ: 0.929572
Ran for 63050 frames Cell Events: 3671 mean_circ: 0.929557
Ran for 64050 frames Cell Events: 3732 mean_circ: 0.92942
Ran for 64550 frames Cell Events: 3763 mean_circ: 0.929351
Ran for 65050 frames Cell Events: 3798 mean_circ: 0.929334
Ran for 66050 frames Cell Events: 3860 mean_circ: 0.929198
Ran for 67550 frames Cell Events: 3952 mean_circ: 0.92916
Ran for 69550 frames Cell Events: 4062 mean_circ: 0.928981
Ran for 70550 frames Cell Events: 4141 mean_circ: 0.928973
Ran for 71050 frames Cell Events: 4178 mean_circ: 0.928943
Ran for 71550 frames Cell Events: 4202 mean_circ: 0.928845
Ran for 72050 frames Cell Events: 4226 mean_circ: 0.928827
Ran for 72550 frames Cell Events: 4260 mean_circ: 0.928839
Ran for 74050 frames Cell Events: 4334 mean_circ: 0.928797
Ran for 75550 frames Cell Events: 4406 mean_circ: 0.928721
Ran for 76050 frames Cell Events: 4439 mean_circ: 0.928622
Ran for 77050 frames Cell Events: 4501 mean_circ: 0.928634
Ran for 77550 frames Cell Events: 4528 mean_circ: 0.928714
Ran for 79550 frames Cell Events: 4646 mean_circ: 0.92878
Ran for 80550 frames Cell Events: 4706 mean_circ: 0.928815
Ran for 84550 frames Cell Events: 4967 mean_circ: 0.928752
Ran for 85550 frames Cell Events: 5044 mean_circ: 0.928751
Ran for 88050 frames Cell Events: 5243 mean_circ: 0.928512
Ran for 88550 frames Cell Events: 5270 mean_circ: 0.928575
Ran for 89050 frames Cell Events: 5300 mean_circ: 0.928603
Ran for 91050 frames Cell Events: 5429 mean_circ: 0.928506
Ran for 92050 frames Cell Events: 5491 mean_circ: 0.928612
Ran for 94050 frames Cell Events: 5625 mean_circ: 0.92833
Ran for 94550 frames Cell Events: 5654 mean_circ: 0.928288
Ran for 95550 frames Cell Events: 5727 mean_circ: 0.928259
Ran for 96550 frames Cell Events: 5794 mean_circ: 0.928261
Ran for 97050 frames Cell Events: 5826 mean_circ: 0.928204
Ran for 97550 frames Cell Events: 5863 mean_circ: 0.928259
Ran for 98050 frames Cell Events: 5891 mean_circ: 0.928303
Ran for 99050 frames Cell Events: 5951 mean_circ: 0.928248
Ran for 102050 frames Cell Events: 6165 mean_circ: 0.928171
Ran for 102550 frames Cell Events: 6199 mean_circ: 0.928193
Ran for 103050 frames Cell Events: 6237 mean_circ: 0.928254
Ran for 103550 frames Cell Events: 6262 mean_circ: 0.928241
Ran for 104550 frames Cell Events: 6324 mean_circ: 0.928155
Ran for 105550 frames Cell Events: 6387 mean_circ: 0.928073
Ran for 107550 frames Cell Events: 6515 mean_circ: 0.928127
Ran for 108550 frames Cell Events: 6588 mean_circ: 0.928125
Ran for 111050 frames Cell Events: 6780 mean_circ: 0.928052
Ran for 113550 frames Cell Events: 6946 mean_circ: 0.928089
Ran for 114550 frames Cell Events: 7005 mean_circ: 0.928132
Ran for 115050 frames Cell Events: 7033 mean_circ: 0.928153
Ran for 115550 frames Cell Events: 7063 mean_circ: 0.928117
Ran for 116050 frames Cell Events: 7089 mean_circ: 0.928131
Ran for 117050 frames Cell Events: 7158 mean_circ: 0.928144
Ran for 117550 frames Cell Events: 7191 mean_circ: 0.928114
Ran for 118550 frames Cell Events: 7273 mean_circ: 0.928075
Ran for 119050 frames Cell Events: 7296 mean_circ: 0.928011
Ran for 119550 frames Cell Events: 7318 mean_circ: 0.928029
Ran for 122050 frames Cell Events: 7464 mean_circ: 0.928053
Ran for 122550 frames Cell Events: 7491 mean_circ: 0.928019
Ran for 123550 frames Cell Events: 7529 mean_circ: 0.928043
Ran for 124550 frames Cell Events: 7570 mean_circ: 0.928029
Ran for 125550 frames Cell Events: 7644 mean_circ: 0.927995
Ran for 126050 frames Cell Events: 7677 mean_circ: 0.927978
Ran for 127550 frames Cell Events: 7792 mean_circ: 0.927864
Ran for 128550 frames Cell Events: 7856 mean_circ: 0.927922
Ran for 129550 frames Cell Events: 7911 mean_circ: 0.927886
Ran for 130050 frames Cell Events: 7947 mean_circ: 0.927882
Ran for 130550 frames Cell Events: 7981 mean_circ: 0.927905
Ran for 131050 frames Cell Events: 8009 mean_circ: 0.92787
Ran for 131550 frames Cell Events: 8042 mean_circ: 0.927866
Ran for 132050 frames Cell Events: 8074 mean_circ: 0.927807
Ran for 133550 frames Cell Events: 8157 mean_circ: 0.927783
Ran for 134050 frames Cell Events: 8184 mean_circ: 0.92772
Ran for 136550 frames Cell Events: 8373 mean_circ: 0.927569
Ran for 137050 frames Cell Events: 8395 mean_circ: 0.92758
Ran for 139550 frames Cell Events: 8547 mean_circ: 0.927368
Ran for 140550 frames Cell Events: 8603 mean_circ: 0.927391
Ran for 141550 frames Cell Events: 8665 mean_circ: 0.927493
Ran for 142050 frames Cell Events: 8697 mean_circ: 0.927523
Ran for 144050 frames Cell Events: 8838 mean_circ: 0.927514
Ran for 144550 frames Cell Events: 8874 mean_circ: 0.927531
Ran for 146050 frames Cell Events: 8963 mean_circ: 0.927612
Ran for 146550 frames Cell Events: 8988 mean_circ: 0.927598
Ran for 147050 frames Cell Events: 9015 mean_circ: 0.92761
Ran for 147550 frames Cell Events: 9049 mean_circ: 0.92761
Ran for 148050 frames Cell Events: 9073 mean_circ: 0.927607
Ran for 148550 frames Cell Events: 9101 mean_circ: 0.927655
Ran for 150050 frames Cell Events: 9173 mean_circ: 0.927582
Ran for 150550 frames Cell Events: 9204 mean_circ: 0.927582
Ran for 151050 frames Cell Events: 9220 mean_circ: 0.927565
Ran for 152050 frames Cell Events: 9277 mean_circ: 0.927606
Ran for 153050 frames Cell Events: 9334 mean_circ: 0.927596
Ran for 153550 frames Cell Events: 9365 mean_circ: 0.927542
Ran for 154050 frames Cell Events: 9400 mean_circ: 0.927542
Ran for 155050 frames Cell Events: 9476 mean_circ: 0.927452
Ran for 156050 frames Cell Events: 9527 mean_circ: 0.927442
Ran for 158050 frames Cell Events: 9677 mean_circ: 0.927393
Ran for 159550 frames Cell Events: 9786 mean_circ: 0.927427
Ran for 160050 frames Cell Events: 9826 mean_circ: 0.927427
Ran for 160550 frames Cell Events: 9853 mean_circ: 0.927427
Ran for 161550 frames Cell Events: 9920 mean_circ: 0.927422
Ran for 162550 frames Cell Events: 9993 mean_circ: 0.927411



End this measurment.
because stop condition (time/nr of cells) was met

Stopping time: 1.27747e+008
****************************************
Measurement finished

Mean Circularitiy: 0.927403
Mean Area: 651.222
Mean FL-1 intensity: 3301.47
Mean FL-2 intensity: 2562
Mean FL-3 intensity: 0

frames processed: 161263
Total Time: 81487.4ms
Number of Runs (new bg img) 0
Frames collected: 162785
Skipped frames: 5799
Rescued Frames: 428327
Samples DAQ: 40696840
Samples written: 81392684
Samples per Channel: 40696342
FPS: 1997.67

Samples per second: 499419
Expected samples/s: 500000

Events: 10002
Events per second: 122.743
jumped back (PMT): 4406
#Frames read, frequency
# 0 frames :262395
# 1 frames :47582
# 2 frames :3875
# 3 frames :1217
# 4 frames :848
# 5 frames :435
# 6 frames :1825
# 7 frames :1231
# 8 frames :901
# 9 frames :540
# 10 frames :872
# 11 frames :492
# 12 frames :349
# 13 frames :229
# 14 frames :283
# 15 frames :243
# 16 frames :231
# 17 frames :176
# 18 frames :116
# 19 frames :113
# 20 frames :105
# 21 frames :99
# 22 frames :80
# 23 frames :64
# 24 frames :66
# 25 frames :53
# 26 frames :51
# 27 frames :42
# 28 frames :45
# 29 frames :47
# 30 frames :43
# 31 frames :26
# 32 frames :31
# 33 frames :20
# 34 frames :19
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# 37 frames :7
# 38 frames :11
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# 40 frames :8
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# 43 frames :5
# 44 frames :11
# 45 frames :7
# 46 frames :3
# 47 frames :1
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# 64 frames :1
# 78 frames :1
M1_para.ini [General]
Measurement Number = 1
Flow Rate [ul/s] = 0.04
Region = "Channel"
Channel width = 20
Current LED = 100

[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 6
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0

#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 24.6888
parameters.txt #fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 6
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 300
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0