Click on the log titles below to expand the log entries present in this DC resource.
dclab-tdms2rtdc
{
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "a0e47e93e9f22858f56a58a6ab901a31",
"name": "M1_20170515_1434_dsRNA_A5_col.tdms"
}
],
"libraries": {
"dclab": "0.59.1",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.6:8b6ee5b, Oct 2 2023 14:57:12",
"implementation": "CPython",
"version": "3.11.6"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-09",
"time": "13:06:53"
}
}
dclab-tdms2rtdc-warnings
LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 303:
Not all features have the same length! Limiting output event count to 8278 (max 8279) in '8278'.
FG_Config.mcf
[Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;
[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;
[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini
[Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6
[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini
[EVENT LOG] fRT-DC V1.06170206
Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 26.1988
Start pulse train for BG Aquisition..
Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 2.23402e+007
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 550 frames Cell Events: 15 mean_circ: 0.945372
Ran for 1050 frames Cell Events: 24 mean_circ: 0.94136
Ran for 1550 frames Cell Events: 41 mean_circ: 0.942283
Ran for 2050 frames Cell Events: 59 mean_circ: 0.94398
Ran for 2550 frames Cell Events: 80 mean_circ: 0.942979
Ran for 3550 frames Cell Events: 118 mean_circ: 0.944398
Ran for 4550 frames Cell Events: 159 mean_circ: 0.945809
Ran for 6050 frames Cell Events: 196 mean_circ: 0.946172
Ran for 6550 frames Cell Events: 211 mean_circ: 0.9461
Ran for 7050 frames Cell Events: 229 mean_circ: 0.945575
Ran for 7550 frames Cell Events: 240 mean_circ: 0.944684
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Ran for 11550 frames Cell Events: 365 mean_circ: 0.946031
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Ran for 12550 frames Cell Events: 390 mean_circ: 0.945738
Ran for 13050 frames Cell Events: 402 mean_circ: 0.945908
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Ran for 14550 frames Cell Events: 442 mean_circ: 0.946512
Ran for 15050 frames Cell Events: 456 mean_circ: 0.946461
Ran for 15550 frames Cell Events: 473 mean_circ: 0.945777
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Ran for 23550 frames Cell Events: 713 mean_circ: 0.945518
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Ran for 33550 frames Cell Events: 1033 mean_circ: 0.945321
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Ran for 36050 frames Cell Events: 1105 mean_circ: 0.945182
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Ran for 37550 frames Cell Events: 1162 mean_circ: 0.944424
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Ran for 40550 frames Cell Events: 1257 mean_circ: 0.94486
Ran for 42050 frames Cell Events: 1315 mean_circ: 0.944335
Ran for 43050 frames Cell Events: 1353 mean_circ: 0.94462
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Ran for 44050 frames Cell Events: 1385 mean_circ: 0.944491
Ran for 45050 frames Cell Events: 1423 mean_circ: 0.944121
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Ran for 47550 frames Cell Events: 1509 mean_circ: 0.943856
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Ran for 48550 frames Cell Events: 1550 mean_circ: 0.943931
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Ran for 50050 frames Cell Events: 1613 mean_circ: 0.943821
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Ran for 63550 frames Cell Events: 2087 mean_circ: 0.943923
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Ran for 73050 frames Cell Events: 2398 mean_circ: 0.944235
Ran for 73550 frames Cell Events: 2422 mean_circ: 0.944219
Ran for 74050 frames Cell Events: 2441 mean_circ: 0.944241
Ran for 74550 frames Cell Events: 2465 mean_circ: 0.944167
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Ran for 76550 frames Cell Events: 2520 mean_circ: 0.944164
Ran for 77550 frames Cell Events: 2552 mean_circ: 0.944187
Ran for 78550 frames Cell Events: 2585 mean_circ: 0.944198
Ran for 79050 frames Cell Events: 2605 mean_circ: 0.944225
Ran for 80050 frames Cell Events: 2640 mean_circ: 0.944215
Ran for 80550 frames Cell Events: 2658 mean_circ: 0.944194
Ran for 81050 frames Cell Events: 2678 mean_circ: 0.944266
Ran for 81550 frames Cell Events: 2693 mean_circ: 0.944272
Ran for 82050 frames Cell Events: 2705 mean_circ: 0.944234
Ran for 82550 frames Cell Events: 2722 mean_circ: 0.94421
Ran for 83050 frames Cell Events: 2736 mean_circ: 0.944174
Ran for 83550 frames Cell Events: 2748 mean_circ: 0.944158
Ran for 84550 frames Cell Events: 2777 mean_circ: 0.944303
Ran for 85550 frames Cell Events: 2807 mean_circ: 0.944398
Ran for 86050 frames Cell Events: 2820 mean_circ: 0.944406
Ran for 86550 frames Cell Events: 2833 mean_circ: 0.944403
Ran for 87050 frames Cell Events: 2844 mean_circ: 0.944378
Ran for 87550 frames Cell Events: 2872 mean_circ: 0.944385
Ran for 88050 frames Cell Events: 2898 mean_circ: 0.944229
Ran for 88550 frames Cell Events: 2925 mean_circ: 0.944285
Ran for 89550 frames Cell Events: 2962 mean_circ: 0.94433
Ran for 90050 frames Cell Events: 2971 mean_circ: 0.944342
Ran for 91050 frames Cell Events: 3011 mean_circ: 0.944287
Ran for 91550 frames Cell Events: 3022 mean_circ: 0.944293
Ran for 92550 frames Cell Events: 3051 mean_circ: 0.944326
Ran for 93050 frames Cell Events: 3066 mean_circ: 0.94436
Ran for 93550 frames Cell Events: 3087 mean_circ: 0.944437
Ran for 94050 frames Cell Events: 3107 mean_circ: 0.944459
Ran for 94550 frames Cell Events: 3122 mean_circ: 0.944447
Ran for 95050 frames Cell Events: 3145 mean_circ: 0.94445
Ran for 98050 frames Cell Events: 3252 mean_circ: 0.944574
Ran for 99550 frames Cell Events: 3304 mean_circ: 0.944595
Ran for 101050 frames Cell Events: 3365 mean_circ: 0.944454
Ran for 102550 frames Cell Events: 3423 mean_circ: 0.944411
Ran for 103050 frames Cell Events: 3443 mean_circ: 0.94439
Ran for 103550 frames Cell Events: 3458 mean_circ: 0.944425
Ran for 104550 frames Cell Events: 3492 mean_circ: 0.944498
Ran for 105050 frames Cell Events: 3513 mean_circ: 0.944529
Ran for 105550 frames Cell Events: 3529 mean_circ: 0.944462
Ran for 107050 frames Cell Events: 3592 mean_circ: 0.944534
Ran for 108050 frames Cell Events: 3612 mean_circ: 0.944598
Ran for 108550 frames Cell Events: 3634 mean_circ: 0.944651
Ran for 109050 frames Cell Events: 3654 mean_circ: 0.944693
Ran for 109550 frames Cell Events: 3670 mean_circ: 0.944758
Ran for 110050 frames Cell Events: 3687 mean_circ: 0.944769
Ran for 110550 frames Cell Events: 3696 mean_circ: 0.944779
Ran for 111050 frames Cell Events: 3710 mean_circ: 0.944842
Ran for 111550 frames Cell Events: 3726 mean_circ: 0.94489
Ran for 112050 frames Cell Events: 3756 mean_circ: 0.944762
Ran for 112550 frames Cell Events: 3770 mean_circ: 0.944747
Ran for 113050 frames Cell Events: 3784 mean_circ: 0.944765
Ran for 113550 frames Cell Events: 3796 mean_circ: 0.944744
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Ran for 121050 frames Cell Events: 4065 mean_circ: 0.944623
Ran for 121550 frames Cell Events: 4082 mean_circ: 0.944642
Ran for 122050 frames Cell Events: 4095 mean_circ: 0.944646
Ran for 123050 frames Cell Events: 4149 mean_circ: 0.944625
Ran for 124550 frames Cell Events: 4210 mean_circ: 0.944574
Ran for 125050 frames Cell Events: 4229 mean_circ: 0.944608
Ran for 125550 frames Cell Events: 4252 mean_circ: 0.944572
Ran for 126050 frames Cell Events: 4262 mean_circ: 0.944569
Ran for 126550 frames Cell Events: 4284 mean_circ: 0.944586
Ran for 128050 frames Cell Events: 4339 mean_circ: 0.94456
Ran for 129550 frames Cell Events: 4393 mean_circ: 0.94457
Ran for 130050 frames Cell Events: 4418 mean_circ: 0.944594
Ran for 130550 frames Cell Events: 4439 mean_circ: 0.944561
Ran for 131050 frames Cell Events: 4451 mean_circ: 0.944546
Ran for 132050 frames Cell Events: 4479 mean_circ: 0.944559
Ran for 132550 frames Cell Events: 4494 mean_circ: 0.944565
Ran for 134050 frames Cell Events: 4550 mean_circ: 0.944506
Ran for 134550 frames Cell Events: 4561 mean_circ: 0.944525
Ran for 135050 frames Cell Events: 4586 mean_circ: 0.944515
Ran for 135550 frames Cell Events: 4612 mean_circ: 0.944453
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Ran for 139050 frames Cell Events: 4771 mean_circ: 0.944411
Ran for 140050 frames Cell Events: 4803 mean_circ: 0.944425
Ran for 140550 frames Cell Events: 4818 mean_circ: 0.944418
Ran for 141050 frames Cell Events: 4846 mean_circ: 0.944466
Ran for 141550 frames Cell Events: 4862 mean_circ: 0.94442
Ran for 142050 frames Cell Events: 4891 mean_circ: 0.944427
Ran for 142550 frames Cell Events: 4904 mean_circ: 0.944361
Ran for 143050 frames Cell Events: 4928 mean_circ: 0.944373
Ran for 143550 frames Cell Events: 4946 mean_circ: 0.944388
Ran for 144550 frames Cell Events: 4983 mean_circ: 0.944298
Ran for 145050 frames Cell Events: 4999 mean_circ: 0.944318
Ran for 145550 frames Cell Events: 5013 mean_circ: 0.94434
Ran for 146550 frames Cell Events: 5038 mean_circ: 0.94433
Ran for 147050 frames Cell Events: 5054 mean_circ: 0.944382
Ran for 148050 frames Cell Events: 5104 mean_circ: 0.944356
Ran for 148550 frames Cell Events: 5112 mean_circ: 0.944358
Ran for 149050 frames Cell Events: 5135 mean_circ: 0.944331
Ran for 149550 frames Cell Events: 5158 mean_circ: 0.944279
Ran for 151550 frames Cell Events: 5227 mean_circ: 0.944176
Ran for 152050 frames Cell Events: 5242 mean_circ: 0.944168
Ran for 154050 frames Cell Events: 5325 mean_circ: 0.944102
Ran for 154550 frames Cell Events: 5344 mean_circ: 0.944143
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Ran for 155550 frames Cell Events: 5377 mean_circ: 0.944204
Ran for 156050 frames Cell Events: 5391 mean_circ: 0.944207
Ran for 156550 frames Cell Events: 5410 mean_circ: 0.944158
Ran for 157050 frames Cell Events: 5429 mean_circ: 0.944169
Ran for 157550 frames Cell Events: 5442 mean_circ: 0.944198
Ran for 158550 frames Cell Events: 5480 mean_circ: 0.94422
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Ran for 162050 frames Cell Events: 5602 mean_circ: 0.944296
Ran for 164550 frames Cell Events: 5715 mean_circ: 0.944294
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Ran for 166050 frames Cell Events: 5772 mean_circ: 0.944447
Ran for 166550 frames Cell Events: 5790 mean_circ: 0.944428
Ran for 167050 frames Cell Events: 5810 mean_circ: 0.944346
Ran for 167550 frames Cell Events: 5821 mean_circ: 0.944297
Ran for 168050 frames Cell Events: 5846 mean_circ: 0.94429
Ran for 168550 frames Cell Events: 5859 mean_circ: 0.944289
Ran for 169050 frames Cell Events: 5875 mean_circ: 0.944282
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Ran for 171050 frames Cell Events: 5950 mean_circ: 0.944262
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Ran for 173050 frames Cell Events: 6039 mean_circ: 0.944217
Ran for 173550 frames Cell Events: 6059 mean_circ: 0.94424
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Ran for 176550 frames Cell Events: 6183 mean_circ: 0.944257
Ran for 178050 frames Cell Events: 6240 mean_circ: 0.944335
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Ran for 187550 frames Cell Events: 6635 mean_circ: 0.944357
Ran for 188050 frames Cell Events: 6658 mean_circ: 0.944407
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Ran for 190050 frames Cell Events: 6731 mean_circ: 0.944405
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Ran for 192050 frames Cell Events: 6812 mean_circ: 0.944421
Ran for 193050 frames Cell Events: 6844 mean_circ: 0.944416
Ran for 193550 frames Cell Events: 6867 mean_circ: 0.944425
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Ran for 195050 frames Cell Events: 6920 mean_circ: 0.944442
Ran for 195550 frames Cell Events: 6938 mean_circ: 0.944417
Ran for 196050 frames Cell Events: 6958 mean_circ: 0.944444
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Ran for 199050 frames Cell Events: 7069 mean_circ: 0.944336
Ran for 200550 frames Cell Events: 7148 mean_circ: 0.944406
Ran for 201550 frames Cell Events: 7197 mean_circ: 0.944377
Ran for 202050 frames Cell Events: 7227 mean_circ: 0.944388
Ran for 202550 frames Cell Events: 7246 mean_circ: 0.944408
Ran for 203050 frames Cell Events: 7267 mean_circ: 0.944389
Ran for 203550 frames Cell Events: 7291 mean_circ: 0.944404
Ran for 204550 frames Cell Events: 7322 mean_circ: 0.944321
Ran for 205050 frames Cell Events: 7339 mean_circ: 0.944305
Ran for 208050 frames Cell Events: 7472 mean_circ: 0.944365
Ran for 208550 frames Cell Events: 7490 mean_circ: 0.944352
Ran for 210050 frames Cell Events: 7563 mean_circ: 0.944377
Ran for 211550 frames Cell Events: 7630 mean_circ: 0.944336
Ran for 212050 frames Cell Events: 7651 mean_circ: 0.944367
Ran for 212550 frames Cell Events: 7675 mean_circ: 0.944378
Ran for 215550 frames Cell Events: 7791 mean_circ: 0.944309
Ran for 216050 frames Cell Events: 7815 mean_circ: 0.944338
Ran for 217050 frames Cell Events: 7852 mean_circ: 0.944394
Ran for 218050 frames Cell Events: 7900 mean_circ: 0.944371
Ran for 220050 frames Cell Events: 7988 mean_circ: 0.944359
Ran for 221050 frames Cell Events: 8023 mean_circ: 0.944346
Ran for 222050 frames Cell Events: 8065 mean_circ: 0.944349
Ran for 222550 frames Cell Events: 8094 mean_circ: 0.944377
Ran for 223050 frames Cell Events: 8117 mean_circ: 0.944386
Ran for 223550 frames Cell Events: 8141 mean_circ: 0.944398
Ran for 224050 frames Cell Events: 8155 mean_circ: 0.944415
Ran for 224550 frames Cell Events: 8180 mean_circ: 0.944416
Ran for 225050 frames Cell Events: 8195 mean_circ: 0.944438
Ran for 225550 frames Cell Events: 8210 mean_circ: 0.944464
Ran for 227050 frames Cell Events: 8262 mean_circ: 0.944444
End this measurment..
Stopping time: 2.24541e+007
****************************************
Measurement finished
Mean Circularitiy: 0.944362
Mean Area: 635.441
Mean FL-1 intensity: 3879.39
Mean FL-2 intensity: 4734.41
Mean FL-3 intensity: 0
frames processed: 225687
Total Time: 113852ms
Number of Runs (new bg img) 0
Frames collected: 227484
Skipped frames: 25653
Rescued Frames: 1130941
Samples DAQ: 56876154
Samples written: 113742112
Samples per Channel: 56871056
FPS: 1998.07
Samples per second: 499518
Expected samples/s: 500000
Events: 8279
Events per second: 72.7174
jumped back (PMT): 3843
#Frames read, frequency
# 0 frames :332071
# 1 frames :106995
# 2 frames :3907
# 3 frames :1558
# 4 frames :873
# 5 frames :473
# 6 frames :1197
# 7 frames :1764
# 8 frames :1151
# 9 frames :704
# 10 frames :535
# 11 frames :734
# 12 frames :539
# 13 frames :341
# 14 frames :277
# 15 frames :234
# 16 frames :176
# 17 frames :179
# 18 frames :173
# 19 frames :165
# 20 frames :184
# 21 frames :141
# 22 frames :108
# 23 frames :81
# 24 frames :46
# 25 frames :41
# 26 frames :43
# 27 frames :33
# 28 frames :30
# 29 frames :24
# 30 frames :20
# 31 frames :18
# 32 frames :9
# 33 frames :10
# 34 frames :12
# 35 frames :10
# 36 frames :9
# 37 frames :9
# 38 frames :3
# 39 frames :7
# 40 frames :5
# 42 frames :6
# 43 frames :8
# 44 frames :1
# 45 frames :2
# 47 frames :2
# 48 frames :1
# 49 frames :2
# 50 frames :3
# 52 frames :3
# 53 frames :2
# 55 frames :1
# 56 frames :1
# 60 frames :2
# 63 frames :1
# 66 frames :1
# 68 frames :1
# 72 frames :1
M1_para.ini
[General]
Measurement Number = 1
Flow Rate [ul/s] = 0.04
Region = "Channel"
Channel width = 20
Current LED = 100
[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0
#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 26.1988
parameters.txt
#fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 300
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0