Click on the log titles below to expand the log entries present in this DC resource.
dclab-tdms2rtdc
{
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "a73c3d97ff12832688b0995529ca7f0f",
"name": "M1_20170515_1624_dsRNA_B5_faster.tdms"
}
],
"libraries": {
"dclab": "0.59.1",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.6:8b6ee5b, Oct 2 2023 14:57:12",
"implementation": "CPython",
"version": "3.11.6"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-09",
"time": "13:14:29"
}
}
dclab-tdms2rtdc-warnings
LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 303:
Not all features have the same length! Limiting output event count to 8624 (max 8625) in '8624'.
FG_Config.mcf
[Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;
[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;
[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini
[Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6
[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini
[EVENT LOG] fRT-DC V1.06170206
Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 26.3317
Start pulse train for BG Aquisition..
Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 2.89252e+007
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 550 frames Cell Events: 19 mean_circ: 0.922142
Ran for 1050 frames Cell Events: 40 mean_circ: 0.930044
Ran for 1550 frames Cell Events: 66 mean_circ: 0.930044
Ran for 2550 frames Cell Events: 99 mean_circ: 0.938239
Ran for 3050 frames Cell Events: 113 mean_circ: 0.937096
Ran for 3550 frames Cell Events: 123 mean_circ: 0.935541
Ran for 4050 frames Cell Events: 150 mean_circ: 0.938358
Ran for 4550 frames Cell Events: 163 mean_circ: 0.937295
Ran for 5550 frames Cell Events: 196 mean_circ: 0.933706
Ran for 7550 frames Cell Events: 262 mean_circ: 0.933235
Ran for 9050 frames Cell Events: 319 mean_circ: 0.934407
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Ran for 15050 frames Cell Events: 544 mean_circ: 0.932168
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Ran for 17050 frames Cell Events: 596 mean_circ: 0.933029
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Ran for 23550 frames Cell Events: 814 mean_circ: 0.931302
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Ran for 35550 frames Cell Events: 1278 mean_circ: 0.93169
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Ran for 37550 frames Cell Events: 1365 mean_circ: 0.931686
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Ran for 39050 frames Cell Events: 1425 mean_circ: 0.931296
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Ran for 40550 frames Cell Events: 1475 mean_circ: 0.930953
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Ran for 42550 frames Cell Events: 1556 mean_circ: 0.931
Ran for 43050 frames Cell Events: 1570 mean_circ: 0.930924
Ran for 43550 frames Cell Events: 1591 mean_circ: 0.930966
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Ran for 53050 frames Cell Events: 1907 mean_circ: 0.931317
Ran for 53550 frames Cell Events: 1931 mean_circ: 0.931345
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Ran for 68550 frames Cell Events: 2521 mean_circ: 0.930652
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Ran for 70050 frames Cell Events: 2577 mean_circ: 0.93009
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Ran for 71050 frames Cell Events: 2604 mean_circ: 0.930188
Ran for 72550 frames Cell Events: 2654 mean_circ: 0.930111
Ran for 73050 frames Cell Events: 2678 mean_circ: 0.930212
Ran for 73550 frames Cell Events: 2700 mean_circ: 0.930071
Ran for 74050 frames Cell Events: 2723 mean_circ: 0.929958
Ran for 74550 frames Cell Events: 2744 mean_circ: 0.929933
Ran for 75050 frames Cell Events: 2762 mean_circ: 0.929816
Ran for 75550 frames Cell Events: 2772 mean_circ: 0.929897
Ran for 76050 frames Cell Events: 2787 mean_circ: 0.929911
Ran for 76550 frames Cell Events: 2804 mean_circ: 0.929846
Ran for 78050 frames Cell Events: 2875 mean_circ: 0.930053
Ran for 78550 frames Cell Events: 2894 mean_circ: 0.930121
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Ran for 81050 frames Cell Events: 2987 mean_circ: 0.930025
Ran for 82050 frames Cell Events: 3030 mean_circ: 0.930071
Ran for 82550 frames Cell Events: 3053 mean_circ: 0.930129
Ran for 85050 frames Cell Events: 3135 mean_circ: 0.929996
Ran for 85550 frames Cell Events: 3147 mean_circ: 0.929989
Ran for 86050 frames Cell Events: 3170 mean_circ: 0.930037
Ran for 86550 frames Cell Events: 3201 mean_circ: 0.930068
Ran for 87050 frames Cell Events: 3223 mean_circ: 0.929945
Ran for 87550 frames Cell Events: 3235 mean_circ: 0.929976
Ran for 88550 frames Cell Events: 3277 mean_circ: 0.929896
Ran for 89550 frames Cell Events: 3305 mean_circ: 0.929918
Ran for 90550 frames Cell Events: 3329 mean_circ: 0.929833
Ran for 91550 frames Cell Events: 3378 mean_circ: 0.929711
Ran for 92050 frames Cell Events: 3389 mean_circ: 0.929742
Ran for 92550 frames Cell Events: 3405 mean_circ: 0.929755
Ran for 93050 frames Cell Events: 3419 mean_circ: 0.929669
Ran for 93550 frames Cell Events: 3433 mean_circ: 0.929572
Ran for 94050 frames Cell Events: 3458 mean_circ: 0.929555
Ran for 94550 frames Cell Events: 3474 mean_circ: 0.929638
Ran for 95050 frames Cell Events: 3491 mean_circ: 0.929638
Ran for 96050 frames Cell Events: 3523 mean_circ: 0.929618
Ran for 96550 frames Cell Events: 3535 mean_circ: 0.929622
Ran for 97550 frames Cell Events: 3573 mean_circ: 0.929681
Ran for 98050 frames Cell Events: 3592 mean_circ: 0.929494
Ran for 99050 frames Cell Events: 3621 mean_circ: 0.929442
Ran for 101050 frames Cell Events: 3707 mean_circ: 0.929399
Ran for 103050 frames Cell Events: 3753 mean_circ: 0.929372
Ran for 103550 frames Cell Events: 3771 mean_circ: 0.929444
Ran for 105050 frames Cell Events: 3817 mean_circ: 0.929368
Ran for 106050 frames Cell Events: 3855 mean_circ: 0.929296
Ran for 107050 frames Cell Events: 3885 mean_circ: 0.929278
Ran for 108050 frames Cell Events: 3930 mean_circ: 0.929463
Ran for 108550 frames Cell Events: 3945 mean_circ: 0.929459
Ran for 109050 frames Cell Events: 3966 mean_circ: 0.92943
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Ran for 110550 frames Cell Events: 4031 mean_circ: 0.929501
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Ran for 112050 frames Cell Events: 4097 mean_circ: 0.929491
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Ran for 122050 frames Cell Events: 4489 mean_circ: 0.929175
Ran for 124050 frames Cell Events: 4549 mean_circ: 0.929204
Ran for 125550 frames Cell Events: 4607 mean_circ: 0.929256
Ran for 126050 frames Cell Events: 4634 mean_circ: 0.929175
Ran for 128050 frames Cell Events: 4693 mean_circ: 0.929115
Ran for 130550 frames Cell Events: 4815 mean_circ: 0.928946
Ran for 131050 frames Cell Events: 4832 mean_circ: 0.928958
Ran for 131550 frames Cell Events: 4860 mean_circ: 0.929
Ran for 132050 frames Cell Events: 4878 mean_circ: 0.928959
Ran for 133050 frames Cell Events: 4927 mean_circ: 0.928954
Ran for 134050 frames Cell Events: 4967 mean_circ: 0.928985
Ran for 134550 frames Cell Events: 4988 mean_circ: 0.92896
Ran for 135050 frames Cell Events: 4996 mean_circ: 0.928909
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Ran for 138050 frames Cell Events: 5112 mean_circ: 0.928964
Ran for 138550 frames Cell Events: 5123 mean_circ: 0.928924
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Ran for 141550 frames Cell Events: 5221 mean_circ: 0.928861
Ran for 142050 frames Cell Events: 5238 mean_circ: 0.928896
Ran for 143050 frames Cell Events: 5286 mean_circ: 0.928907
Ran for 143550 frames Cell Events: 5305 mean_circ: 0.92892
Ran for 146550 frames Cell Events: 5428 mean_circ: 0.92895
Ran for 147050 frames Cell Events: 5455 mean_circ: 0.928911
Ran for 147550 frames Cell Events: 5470 mean_circ: 0.928839
Ran for 148050 frames Cell Events: 5488 mean_circ: 0.928837
Ran for 149050 frames Cell Events: 5521 mean_circ: 0.928854
Ran for 151050 frames Cell Events: 5602 mean_circ: 0.928915
Ran for 151550 frames Cell Events: 5626 mean_circ: 0.928925
Ran for 152050 frames Cell Events: 5649 mean_circ: 0.928925
Ran for 152550 frames Cell Events: 5660 mean_circ: 0.928926
Ran for 153550 frames Cell Events: 5683 mean_circ: 0.928999
Ran for 154550 frames Cell Events: 5720 mean_circ: 0.928969
Ran for 155550 frames Cell Events: 5762 mean_circ: 0.928949
Ran for 156050 frames Cell Events: 5785 mean_circ: 0.928889
Ran for 156550 frames Cell Events: 5802 mean_circ: 0.928891
Ran for 157050 frames Cell Events: 5823 mean_circ: 0.928923
Ran for 158050 frames Cell Events: 5859 mean_circ: 0.928858
Ran for 158550 frames Cell Events: 5879 mean_circ: 0.92887
Ran for 160050 frames Cell Events: 5926 mean_circ: 0.928918
Ran for 160550 frames Cell Events: 5941 mean_circ: 0.928934
Ran for 161050 frames Cell Events: 5961 mean_circ: 0.928946
Ran for 161550 frames Cell Events: 5979 mean_circ: 0.92892
Ran for 162050 frames Cell Events: 5996 mean_circ: 0.928871
Ran for 162550 frames Cell Events: 6012 mean_circ: 0.928874
Ran for 163050 frames Cell Events: 6029 mean_circ: 0.928933
Ran for 164050 frames Cell Events: 6072 mean_circ: 0.92892
Ran for 165550 frames Cell Events: 6137 mean_circ: 0.928936
Ran for 167050 frames Cell Events: 6201 mean_circ: 0.928912
Ran for 167550 frames Cell Events: 6219 mean_circ: 0.928909
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Ran for 168550 frames Cell Events: 6248 mean_circ: 0.928977
Ran for 169050 frames Cell Events: 6262 mean_circ: 0.929003
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Ran for 172550 frames Cell Events: 6393 mean_circ: 0.92888
Ran for 173550 frames Cell Events: 6437 mean_circ: 0.928891
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Ran for 201550 frames Cell Events: 7459 mean_circ: 0.928832
Ran for 202050 frames Cell Events: 7481 mean_circ: 0.928807
Ran for 203550 frames Cell Events: 7534 mean_circ: 0.928897
Ran for 204050 frames Cell Events: 7546 mean_circ: 0.928903
Ran for 204550 frames Cell Events: 7565 mean_circ: 0.928897
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Ran for 205550 frames Cell Events: 7605 mean_circ: 0.928826
Ran for 206050 frames Cell Events: 7622 mean_circ: 0.928811
Ran for 207550 frames Cell Events: 7683 mean_circ: 0.928803
Ran for 208050 frames Cell Events: 7695 mean_circ: 0.928774
Ran for 208550 frames Cell Events: 7712 mean_circ: 0.92876
Ran for 209050 frames Cell Events: 7733 mean_circ: 0.92874
Ran for 209550 frames Cell Events: 7750 mean_circ: 0.928764
Ran for 210050 frames Cell Events: 7759 mean_circ: 0.928771
Ran for 210550 frames Cell Events: 7783 mean_circ: 0.928796
Ran for 211050 frames Cell Events: 7800 mean_circ: 0.928776
Ran for 211550 frames Cell Events: 7822 mean_circ: 0.928759
Ran for 212050 frames Cell Events: 7839 mean_circ: 0.928761
Ran for 212550 frames Cell Events: 7853 mean_circ: 0.928774
Ran for 213050 frames Cell Events: 7870 mean_circ: 0.928787
Ran for 213550 frames Cell Events: 7889 mean_circ: 0.928762
Ran for 214550 frames Cell Events: 7935 mean_circ: 0.928767
Ran for 215050 frames Cell Events: 7944 mean_circ: 0.928773
Ran for 215550 frames Cell Events: 7953 mean_circ: 0.928771
Ran for 216050 frames Cell Events: 7983 mean_circ: 0.928759
Ran for 216550 frames Cell Events: 7992 mean_circ: 0.928753
Ran for 217050 frames Cell Events: 8015 mean_circ: 0.928732
Ran for 217550 frames Cell Events: 8038 mean_circ: 0.928695
Ran for 218050 frames Cell Events: 8067 mean_circ: 0.928648
Ran for 219050 frames Cell Events: 8107 mean_circ: 0.928613
Ran for 219550 frames Cell Events: 8123 mean_circ: 0.92862
Ran for 220050 frames Cell Events: 8136 mean_circ: 0.928614
Ran for 220550 frames Cell Events: 8159 mean_circ: 0.928592
Ran for 221050 frames Cell Events: 8178 mean_circ: 0.928601
Ran for 222050 frames Cell Events: 8228 mean_circ: 0.928651
Ran for 222550 frames Cell Events: 8242 mean_circ: 0.92864
Ran for 223050 frames Cell Events: 8264 mean_circ: 0.9286
Ran for 224050 frames Cell Events: 8305 mean_circ: 0.928611
Ran for 225050 frames Cell Events: 8340 mean_circ: 0.928606
Ran for 225550 frames Cell Events: 8367 mean_circ: 0.928553
Ran for 226550 frames Cell Events: 8405 mean_circ: 0.928544
Ran for 227550 frames Cell Events: 8423 mean_circ: 0.928555
Ran for 228050 frames Cell Events: 8439 mean_circ: 0.928573
Ran for 228550 frames Cell Events: 8460 mean_circ: 0.928546
Ran for 229050 frames Cell Events: 8491 mean_circ: 0.928532
Ran for 229550 frames Cell Events: 8504 mean_circ: 0.928563
Ran for 231050 frames Cell Events: 8584 mean_circ: 0.928619
End this measurment..
Stopping time: 2.90411e+007
****************************************
Measurement finished
Mean Circularitiy: 0.928585
Mean Area: 685.035
Mean FL-1 intensity: 3654.44
Mean FL-2 intensity: 5084.22
Mean FL-3 intensity: 0
frames processed: 228998
Total Time: 115895ms
Number of Runs (new bg img) 0
Frames collected: 231656
Skipped frames: 54271
Rescued Frames: 2744947
Samples DAQ: 57916697
Samples written: 115827782
Samples per Channel: 57913891
FPS: 1998.85
Samples per second: 499712
Expected samples/s: 500000
Events: 8625
Events per second: 74.4211
jumped back (PMT): 20
#Frames read, frequency
# 0 frames :330132
# 1 frames :116586
# 2 frames :4221
# 3 frames :1593
# 4 frames :824
# 5 frames :352
# 6 frames :2216
# 7 frames :1883
# 8 frames :1340
# 9 frames :772
# 10 frames :519
# 11 frames :522
# 12 frames :454
# 13 frames :286
# 14 frames :274
# 15 frames :217
# 16 frames :165
# 17 frames :140
# 18 frames :126
# 19 frames :96
# 20 frames :98
# 21 frames :77
# 22 frames :77
# 23 frames :44
# 24 frames :39
# 25 frames :32
# 26 frames :27
# 27 frames :32
# 28 frames :13
# 29 frames :15
# 30 frames :16
# 31 frames :10
# 32 frames :10
# 33 frames :4
# 34 frames :7
# 35 frames :5
# 36 frames :5
# 37 frames :4
# 38 frames :3
# 39 frames :4
# 40 frames :4
# 41 frames :2
# 42 frames :4
# 43 frames :1
# 44 frames :5
# 45 frames :2
# 46 frames :2
# 48 frames :1
# 49 frames :1
# 50 frames :2
# 51 frames :1
# 52 frames :1
# 53 frames :1
# 54 frames :1
# 55 frames :1
# 56 frames :1
# 58 frames :1
# 59 frames :2
# 79 frames :1
M1_para.ini
[General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100
[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0
#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 26.3317
parameters.txt
#fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0