Click on the log titles below to expand the log entries present in this DC resource.
dclab-tdms2rtdc
{
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "c3e8fd53390751b5bc9a5de5155362b0",
"name": "M1_20170515_1844_dsRNA_C7_faster.tdms"
}
],
"libraries": {
"dclab": "0.59.1",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.6:8b6ee5b, Oct 2 2023 14:57:12",
"implementation": "CPython",
"version": "3.11.6"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-09",
"time": "13:24:48"
}
}
dclab-tdms2rtdc-warnings
LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 303:
Not all features have the same length! Limiting output event count to 8371 (max 8372) in '8371'.
FG_Config.mcf
[Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;
[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;
[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini
[Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6
[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini
[EVENT LOG] fRT-DC V1.06170206
Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 26.2902
Start pulse train for BG Aquisition..
Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 3.73144e+007
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 550 frames Cell Events: 20 mean_circ: 0.935563
Ran for 1050 frames Cell Events: 59 mean_circ: 0.935013
Ran for 2550 frames Cell Events: 126 mean_circ: 0.939693
Ran for 3050 frames Cell Events: 154 mean_circ: 0.937488
Ran for 4050 frames Cell Events: 236 mean_circ: 0.935607
Ran for 5050 frames Cell Events: 271 mean_circ: 0.936378
Ran for 5550 frames Cell Events: 294 mean_circ: 0.936301
Ran for 7050 frames Cell Events: 365 mean_circ: 0.936901
Ran for 7550 frames Cell Events: 387 mean_circ: 0.936853
Ran for 9550 frames Cell Events: 473 mean_circ: 0.93691
Ran for 10050 frames Cell Events: 497 mean_circ: 0.937121
Ran for 10550 frames Cell Events: 530 mean_circ: 0.937254
Ran for 11050 frames Cell Events: 545 mean_circ: 0.937693
Ran for 12550 frames Cell Events: 621 mean_circ: 0.937115
Ran for 13050 frames Cell Events: 653 mean_circ: 0.936267
Ran for 15550 frames Cell Events: 793 mean_circ: 0.935914
Ran for 17550 frames Cell Events: 915 mean_circ: 0.936357
Ran for 18550 frames Cell Events: 986 mean_circ: 0.936716
Ran for 19550 frames Cell Events: 1041 mean_circ: 0.936669
Ran for 20050 frames Cell Events: 1064 mean_circ: 0.937101
Ran for 20550 frames Cell Events: 1087 mean_circ: 0.936674
Ran for 21550 frames Cell Events: 1144 mean_circ: 0.93691
Ran for 22050 frames Cell Events: 1170 mean_circ: 0.936878
Ran for 22550 frames Cell Events: 1195 mean_circ: 0.937042
Ran for 23550 frames Cell Events: 1243 mean_circ: 0.937089
Ran for 24550 frames Cell Events: 1284 mean_circ: 0.936855
Ran for 26050 frames Cell Events: 1358 mean_circ: 0.936837
Ran for 29050 frames Cell Events: 1530 mean_circ: 0.936153
Ran for 29550 frames Cell Events: 1560 mean_circ: 0.936092
Ran for 30050 frames Cell Events: 1577 mean_circ: 0.936165
Ran for 30550 frames Cell Events: 1602 mean_circ: 0.936213
Ran for 31050 frames Cell Events: 1626 mean_circ: 0.936303
Ran for 34050 frames Cell Events: 1781 mean_circ: 0.935924
Ran for 34550 frames Cell Events: 1810 mean_circ: 0.935952
Ran for 35550 frames Cell Events: 1853 mean_circ: 0.93596
Ran for 36050 frames Cell Events: 1884 mean_circ: 0.93587
Ran for 37550 frames Cell Events: 1952 mean_circ: 0.935981
Ran for 38050 frames Cell Events: 1970 mean_circ: 0.936052
Ran for 38550 frames Cell Events: 2004 mean_circ: 0.935866
Ran for 40550 frames Cell Events: 2138 mean_circ: 0.93609
Ran for 41050 frames Cell Events: 2165 mean_circ: 0.936088
Ran for 42050 frames Cell Events: 2212 mean_circ: 0.936048
Ran for 43550 frames Cell Events: 2292 mean_circ: 0.93613
Ran for 44550 frames Cell Events: 2356 mean_circ: 0.935899
Ran for 45050 frames Cell Events: 2381 mean_circ: 0.935915
Ran for 45550 frames Cell Events: 2396 mean_circ: 0.935983
Ran for 46050 frames Cell Events: 2414 mean_circ: 0.936027
Ran for 46550 frames Cell Events: 2453 mean_circ: 0.936139
Ran for 47050 frames Cell Events: 2478 mean_circ: 0.936215
Ran for 47550 frames Cell Events: 2494 mean_circ: 0.936222
Ran for 48050 frames Cell Events: 2513 mean_circ: 0.936276
Ran for 48550 frames Cell Events: 2540 mean_circ: 0.936269
Ran for 49550 frames Cell Events: 2598 mean_circ: 0.936213
Ran for 50550 frames Cell Events: 2649 mean_circ: 0.936236
Ran for 51050 frames Cell Events: 2685 mean_circ: 0.936256
Ran for 52050 frames Cell Events: 2746 mean_circ: 0.936196
Ran for 52550 frames Cell Events: 2764 mean_circ: 0.936118
Ran for 53050 frames Cell Events: 2785 mean_circ: 0.93616
Ran for 54050 frames Cell Events: 2834 mean_circ: 0.936258
Ran for 55550 frames Cell Events: 2923 mean_circ: 0.936154
Ran for 56050 frames Cell Events: 2967 mean_circ: 0.9362
Ran for 56550 frames Cell Events: 2983 mean_circ: 0.936171
Ran for 57550 frames Cell Events: 3045 mean_circ: 0.936267
Ran for 58550 frames Cell Events: 3087 mean_circ: 0.936114
Ran for 59550 frames Cell Events: 3143 mean_circ: 0.936007
Ran for 61050 frames Cell Events: 3215 mean_circ: 0.936153
Ran for 61550 frames Cell Events: 3238 mean_circ: 0.936058
Ran for 62550 frames Cell Events: 3300 mean_circ: 0.93598
Ran for 63550 frames Cell Events: 3343 mean_circ: 0.935894
Ran for 65050 frames Cell Events: 3417 mean_circ: 0.935974
Ran for 66050 frames Cell Events: 3470 mean_circ: 0.936004
Ran for 66550 frames Cell Events: 3511 mean_circ: 0.935932
Ran for 68050 frames Cell Events: 3624 mean_circ: 0.935906
Ran for 69550 frames Cell Events: 3726 mean_circ: 0.93597
Ran for 70050 frames Cell Events: 3763 mean_circ: 0.935903
Ran for 70550 frames Cell Events: 3787 mean_circ: 0.935932
Ran for 71050 frames Cell Events: 3805 mean_circ: 0.935879
Ran for 72550 frames Cell Events: 3897 mean_circ: 0.935977
Ran for 73550 frames Cell Events: 3955 mean_circ: 0.935835
Ran for 74050 frames Cell Events: 3979 mean_circ: 0.935758
Ran for 74550 frames Cell Events: 3994 mean_circ: 0.935704
Ran for 75050 frames Cell Events: 4017 mean_circ: 0.935738
Ran for 76050 frames Cell Events: 4076 mean_circ: 0.93567
Ran for 76550 frames Cell Events: 4100 mean_circ: 0.935583
Ran for 77550 frames Cell Events: 4163 mean_circ: 0.935514
Ran for 78050 frames Cell Events: 4191 mean_circ: 0.935531
Ran for 78550 frames Cell Events: 4215 mean_circ: 0.935519
Ran for 79550 frames Cell Events: 4269 mean_circ: 0.935539
Ran for 80550 frames Cell Events: 4328 mean_circ: 0.935434
Ran for 81050 frames Cell Events: 4352 mean_circ: 0.935507
Ran for 82050 frames Cell Events: 4414 mean_circ: 0.935609
Ran for 82550 frames Cell Events: 4441 mean_circ: 0.93561
Ran for 83050 frames Cell Events: 4463 mean_circ: 0.935666
Ran for 83550 frames Cell Events: 4497 mean_circ: 0.935675
Ran for 84550 frames Cell Events: 4558 mean_circ: 0.935681
Ran for 86550 frames Cell Events: 4633 mean_circ: 0.935643
Ran for 87050 frames Cell Events: 4657 mean_circ: 0.935633
Ran for 87550 frames Cell Events: 4686 mean_circ: 0.935668
Ran for 89050 frames Cell Events: 4780 mean_circ: 0.935788
Ran for 90050 frames Cell Events: 4827 mean_circ: 0.935769
Ran for 90550 frames Cell Events: 4848 mean_circ: 0.935788
Ran for 93550 frames Cell Events: 4973 mean_circ: 0.935827
Ran for 94550 frames Cell Events: 5027 mean_circ: 0.935882
Ran for 96550 frames Cell Events: 5143 mean_circ: 0.935857
Ran for 99550 frames Cell Events: 5293 mean_circ: 0.93584
Ran for 100550 frames Cell Events: 5351 mean_circ: 0.935862
Ran for 101050 frames Cell Events: 5381 mean_circ: 0.935814
Ran for 101550 frames Cell Events: 5406 mean_circ: 0.935795
Ran for 103050 frames Cell Events: 5485 mean_circ: 0.935786
Ran for 103550 frames Cell Events: 5519 mean_circ: 0.935746
Ran for 104050 frames Cell Events: 5551 mean_circ: 0.935703
Ran for 104550 frames Cell Events: 5580 mean_circ: 0.935731
Ran for 105050 frames Cell Events: 5611 mean_circ: 0.935679
Ran for 106050 frames Cell Events: 5664 mean_circ: 0.935597
Ran for 106550 frames Cell Events: 5686 mean_circ: 0.935534
Ran for 107050 frames Cell Events: 5712 mean_circ: 0.935529
Ran for 108550 frames Cell Events: 5817 mean_circ: 0.935545
Ran for 109550 frames Cell Events: 5892 mean_circ: 0.935605
Ran for 110050 frames Cell Events: 5912 mean_circ: 0.935599
Ran for 111050 frames Cell Events: 5971 mean_circ: 0.935481
Ran for 111550 frames Cell Events: 5994 mean_circ: 0.93555
Ran for 113050 frames Cell Events: 6075 mean_circ: 0.935519
Ran for 114050 frames Cell Events: 6142 mean_circ: 0.935553
Ran for 114550 frames Cell Events: 6163 mean_circ: 0.935564
Ran for 115050 frames Cell Events: 6195 mean_circ: 0.935567
Ran for 116050 frames Cell Events: 6244 mean_circ: 0.935587
Ran for 117050 frames Cell Events: 6302 mean_circ: 0.935652
Ran for 117550 frames Cell Events: 6328 mean_circ: 0.935639
Ran for 118050 frames Cell Events: 6351 mean_circ: 0.935661
Ran for 119550 frames Cell Events: 6437 mean_circ: 0.935634
Ran for 120050 frames Cell Events: 6456 mean_circ: 0.935614
Ran for 122550 frames Cell Events: 6584 mean_circ: 0.935498
Ran for 123050 frames Cell Events: 6605 mean_circ: 0.935505
Ran for 124050 frames Cell Events: 6652 mean_circ: 0.935523
Ran for 124550 frames Cell Events: 6676 mean_circ: 0.935563
Ran for 125050 frames Cell Events: 6702 mean_circ: 0.935578
Ran for 129050 frames Cell Events: 6912 mean_circ: 0.935573
Ran for 132550 frames Cell Events: 7105 mean_circ: 0.935619
Ran for 134050 frames Cell Events: 7199 mean_circ: 0.935586
Ran for 134550 frames Cell Events: 7240 mean_circ: 0.935586
Ran for 136550 frames Cell Events: 7349 mean_circ: 0.935643
Ran for 137550 frames Cell Events: 7407 mean_circ: 0.935582
Ran for 139550 frames Cell Events: 7533 mean_circ: 0.935398
Ran for 140050 frames Cell Events: 7555 mean_circ: 0.935349
Ran for 141550 frames Cell Events: 7647 mean_circ: 0.935461
Ran for 142050 frames Cell Events: 7671 mean_circ: 0.935527
Ran for 142550 frames Cell Events: 7701 mean_circ: 0.935525
Ran for 143050 frames Cell Events: 7725 mean_circ: 0.93553
Ran for 143550 frames Cell Events: 7747 mean_circ: 0.935592
Ran for 145050 frames Cell Events: 7831 mean_circ: 0.935631
Ran for 146050 frames Cell Events: 7868 mean_circ: 0.935601
Ran for 150050 frames Cell Events: 8111 mean_circ: 0.935519
Ran for 150550 frames Cell Events: 8132 mean_circ: 0.935526
Ran for 151550 frames Cell Events: 8182 mean_circ: 0.935553
Ran for 152550 frames Cell Events: 8237 mean_circ: 0.935545
Ran for 153050 frames Cell Events: 8264 mean_circ: 0.935528
Ran for 154050 frames Cell Events: 8315 mean_circ: 0.935568
End this measurment..
Stopping time: 3.73921e+007
****************************************
Measurement finished
Mean Circularitiy: 0.935589
Mean Area: 638.262
Mean FL-1 intensity: 3980.23
Mean FL-2 intensity: 5607.53
Mean FL-3 intensity: 0
frames processed: 152679
Total Time: 77637.1ms
Number of Runs (new bg img) 0
Frames collected: 155031
Skipped frames: 102631
Rescued Frames: 4744714
Samples DAQ: 38762902
Samples written: 77515530
Samples per Channel: 38757765
FPS: 1996.87
Samples per second: 499217
Expected samples/s: 500000
Events: 8372
Events per second: 107.835
jumped back (PMT): 23
#Frames read, frequency
# 0 frames :236960
# 1 frames :59762
# 2 frames :2766
# 3 frames :935
# 4 frames :497
# 5 frames :265
# 6 frames :2056
# 7 frames :1146
# 8 frames :1041
# 9 frames :607
# 10 frames :494
# 11 frames :327
# 12 frames :308
# 13 frames :213
# 14 frames :214
# 15 frames :186
# 16 frames :155
# 17 frames :149
# 18 frames :115
# 19 frames :94
# 20 frames :90
# 21 frames :69
# 22 frames :53
# 23 frames :60
# 24 frames :47
# 25 frames :31
# 26 frames :37
# 27 frames :32
# 28 frames :26
# 29 frames :26
# 30 frames :19
# 31 frames :21
# 32 frames :9
# 33 frames :18
# 34 frames :11
# 35 frames :11
# 36 frames :3
# 37 frames :10
# 38 frames :11
# 39 frames :6
# 40 frames :6
# 41 frames :4
# 42 frames :7
# 43 frames :4
# 44 frames :5
# 45 frames :5
# 46 frames :8
# 47 frames :3
# 48 frames :3
# 49 frames :5
# 50 frames :5
# 52 frames :1
# 53 frames :2
# 54 frames :1
# 55 frames :5
# 58 frames :1
# 60 frames :1
# 61 frames :2
# 62 frames :1
# 63 frames :1
# 68 frames :2
# 71 frames :1
# 77 frames :1
# 88 frames :2
M1_para.ini
[General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100
[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0
#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 26.2902
parameters.txt
#fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0