Click on the log titles below to expand the log entries present in this DC resource.
dclab-tdms2rtdc
{
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "d21c5e1c8d648db1127f40955e2b852f",
"name": "M1_20170515_1907_dsRNA_D1_faster.tdms"
}
],
"libraries": {
"dclab": "0.59.1",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.6:8b6ee5b, Oct 2 2023 14:57:12",
"implementation": "CPython",
"version": "3.11.6"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-09",
"time": "13:26:24"
}
}
dclab-tdms2rtdc-warnings
LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 303:
Not all features have the same length! Limiting output event count to 8036 (max 8037) in '8036'.
FG_Config.mcf
[Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;
[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;
[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini
[Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6
[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini
[EVENT LOG] fRT-DC V1.06170206
Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 26.361
Start pulse train for BG Aquisition..
Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 3.87225e+007
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 550 frames Cell Events: 38 mean_circ: 0.910175
Ran for 1050 frames Cell Events: 63 mean_circ: 0.923476
Ran for 2050 frames Cell Events: 118 mean_circ: 0.927437
Ran for 2550 frames Cell Events: 142 mean_circ: 0.926938
Ran for 4550 frames Cell Events: 257 mean_circ: 0.927268
Ran for 5050 frames Cell Events: 295 mean_circ: 0.929657
Ran for 5550 frames Cell Events: 319 mean_circ: 0.93018
Ran for 6550 frames Cell Events: 374 mean_circ: 0.930354
Ran for 7050 frames Cell Events: 396 mean_circ: 0.92796
Ran for 9050 frames Cell Events: 492 mean_circ: 0.928286
Ran for 10050 frames Cell Events: 535 mean_circ: 0.92924
Ran for 10550 frames Cell Events: 553 mean_circ: 0.929639
Ran for 11050 frames Cell Events: 579 mean_circ: 0.930388
Ran for 11550 frames Cell Events: 600 mean_circ: 0.930783
Ran for 12550 frames Cell Events: 653 mean_circ: 0.931944
Ran for 13050 frames Cell Events: 670 mean_circ: 0.931842
Ran for 13550 frames Cell Events: 705 mean_circ: 0.932263
Ran for 15550 frames Cell Events: 819 mean_circ: 0.931748
Ran for 17050 frames Cell Events: 909 mean_circ: 0.931644
Ran for 18050 frames Cell Events: 965 mean_circ: 0.93171
Ran for 18550 frames Cell Events: 990 mean_circ: 0.931304
Ran for 19050 frames Cell Events: 1007 mean_circ: 0.931068
Ran for 20050 frames Cell Events: 1059 mean_circ: 0.931116
Ran for 21050 frames Cell Events: 1117 mean_circ: 0.931208
Ran for 21550 frames Cell Events: 1141 mean_circ: 0.930813
Ran for 23050 frames Cell Events: 1227 mean_circ: 0.93123
Ran for 23550 frames Cell Events: 1253 mean_circ: 0.931259
Ran for 24050 frames Cell Events: 1281 mean_circ: 0.931014
Ran for 24550 frames Cell Events: 1313 mean_circ: 0.93105
Ran for 25050 frames Cell Events: 1331 mean_circ: 0.931416
Ran for 26050 frames Cell Events: 1383 mean_circ: 0.931441
Ran for 27550 frames Cell Events: 1452 mean_circ: 0.931845
Ran for 28550 frames Cell Events: 1500 mean_circ: 0.931768
Ran for 29550 frames Cell Events: 1549 mean_circ: 0.93194
Ran for 30550 frames Cell Events: 1605 mean_circ: 0.931865
Ran for 32050 frames Cell Events: 1683 mean_circ: 0.931614
Ran for 33050 frames Cell Events: 1731 mean_circ: 0.931777
Ran for 34550 frames Cell Events: 1777 mean_circ: 0.931734
Ran for 36050 frames Cell Events: 1843 mean_circ: 0.93179
Ran for 36550 frames Cell Events: 1860 mean_circ: 0.931572
Ran for 38050 frames Cell Events: 1925 mean_circ: 0.93133
Ran for 38550 frames Cell Events: 1954 mean_circ: 0.930814
Ran for 40050 frames Cell Events: 2038 mean_circ: 0.930727
Ran for 41050 frames Cell Events: 2081 mean_circ: 0.930624
Ran for 41550 frames Cell Events: 2095 mean_circ: 0.930754
Ran for 43550 frames Cell Events: 2190 mean_circ: 0.93093
Ran for 44050 frames Cell Events: 2214 mean_circ: 0.930982
Ran for 46050 frames Cell Events: 2332 mean_circ: 0.930947
Ran for 47550 frames Cell Events: 2444 mean_circ: 0.930762
Ran for 48050 frames Cell Events: 2473 mean_circ: 0.930853
Ran for 48550 frames Cell Events: 2507 mean_circ: 0.931084
Ran for 51050 frames Cell Events: 2662 mean_circ: 0.931148
Ran for 53550 frames Cell Events: 2783 mean_circ: 0.931074
Ran for 54550 frames Cell Events: 2800 mean_circ: 0.931076
Ran for 55050 frames Cell Events: 2829 mean_circ: 0.931083
Ran for 55550 frames Cell Events: 2856 mean_circ: 0.931077
Ran for 57550 frames Cell Events: 2953 mean_circ: 0.931103
Ran for 58050 frames Cell Events: 2979 mean_circ: 0.931084
Ran for 58550 frames Cell Events: 2995 mean_circ: 0.931148
Ran for 59050 frames Cell Events: 3009 mean_circ: 0.931169
Ran for 61550 frames Cell Events: 3167 mean_circ: 0.931135
Ran for 62050 frames Cell Events: 3192 mean_circ: 0.931228
Ran for 63050 frames Cell Events: 3242 mean_circ: 0.931141
Ran for 63550 frames Cell Events: 3273 mean_circ: 0.931148
Ran for 64050 frames Cell Events: 3296 mean_circ: 0.931082
Ran for 64550 frames Cell Events: 3316 mean_circ: 0.931178
Ran for 65550 frames Cell Events: 3392 mean_circ: 0.930999
Ran for 66050 frames Cell Events: 3414 mean_circ: 0.931101
Ran for 66550 frames Cell Events: 3437 mean_circ: 0.931235
Ran for 67050 frames Cell Events: 3466 mean_circ: 0.931192
Ran for 67550 frames Cell Events: 3485 mean_circ: 0.931237
Ran for 69050 frames Cell Events: 3545 mean_circ: 0.931203
Ran for 69550 frames Cell Events: 3566 mean_circ: 0.931135
Ran for 70050 frames Cell Events: 3598 mean_circ: 0.931019
Ran for 70550 frames Cell Events: 3617 mean_circ: 0.931042
Ran for 73050 frames Cell Events: 3767 mean_circ: 0.93119
Ran for 73550 frames Cell Events: 3797 mean_circ: 0.931059
Ran for 74050 frames Cell Events: 3821 mean_circ: 0.931062
Ran for 74550 frames Cell Events: 3841 mean_circ: 0.931016
Ran for 76050 frames Cell Events: 3913 mean_circ: 0.931089
Ran for 77550 frames Cell Events: 3962 mean_circ: 0.931025
Ran for 79550 frames Cell Events: 4065 mean_circ: 0.931049
Ran for 80050 frames Cell Events: 4098 mean_circ: 0.931037
Ran for 82050 frames Cell Events: 4212 mean_circ: 0.930869
Ran for 83550 frames Cell Events: 4312 mean_circ: 0.930805
Ran for 85050 frames Cell Events: 4409 mean_circ: 0.930741
Ran for 86550 frames Cell Events: 4508 mean_circ: 0.930817
Ran for 87550 frames Cell Events: 4579 mean_circ: 0.930861
Ran for 88550 frames Cell Events: 4637 mean_circ: 0.930881
Ran for 89050 frames Cell Events: 4657 mean_circ: 0.93093
Ran for 91550 frames Cell Events: 4842 mean_circ: 0.931031
Ran for 92550 frames Cell Events: 4891 mean_circ: 0.931144
Ran for 94050 frames Cell Events: 4962 mean_circ: 0.931108
Ran for 94550 frames Cell Events: 4985 mean_circ: 0.931081
Ran for 95050 frames Cell Events: 5004 mean_circ: 0.931055
Ran for 96050 frames Cell Events: 5076 mean_circ: 0.931093
Ran for 97050 frames Cell Events: 5122 mean_circ: 0.93107
Ran for 98050 frames Cell Events: 5185 mean_circ: 0.930911
Ran for 98550 frames Cell Events: 5208 mean_circ: 0.930904
Ran for 99550 frames Cell Events: 5272 mean_circ: 0.930912
Ran for 100550 frames Cell Events: 5310 mean_circ: 0.931014
Ran for 101050 frames Cell Events: 5357 mean_circ: 0.931014
Ran for 102550 frames Cell Events: 5430 mean_circ: 0.931041
Ran for 103550 frames Cell Events: 5486 mean_circ: 0.930939
Ran for 106050 frames Cell Events: 5608 mean_circ: 0.931019
Ran for 106550 frames Cell Events: 5621 mean_circ: 0.931052
Ran for 107050 frames Cell Events: 5660 mean_circ: 0.931097
Ran for 107550 frames Cell Events: 5690 mean_circ: 0.93109
Ran for 108050 frames Cell Events: 5728 mean_circ: 0.931089
Ran for 109050 frames Cell Events: 5808 mean_circ: 0.931096
Ran for 109550 frames Cell Events: 5834 mean_circ: 0.931157
Ran for 110550 frames Cell Events: 5882 mean_circ: 0.9312
Ran for 112050 frames Cell Events: 5960 mean_circ: 0.9312
Ran for 113550 frames Cell Events: 6023 mean_circ: 0.931304
Ran for 114050 frames Cell Events: 6043 mean_circ: 0.931284
Ran for 114550 frames Cell Events: 6063 mean_circ: 0.9313
Ran for 115550 frames Cell Events: 6139 mean_circ: 0.931401
Ran for 116050 frames Cell Events: 6163 mean_circ: 0.931392
Ran for 116550 frames Cell Events: 6182 mean_circ: 0.931441
Ran for 117050 frames Cell Events: 6212 mean_circ: 0.931488
Ran for 117550 frames Cell Events: 6246 mean_circ: 0.931441
Ran for 118050 frames Cell Events: 6269 mean_circ: 0.9315
Ran for 118550 frames Cell Events: 6290 mean_circ: 0.931521
Ran for 119050 frames Cell Events: 6308 mean_circ: 0.931519
Ran for 119550 frames Cell Events: 6335 mean_circ: 0.931539
Ran for 120550 frames Cell Events: 6389 mean_circ: 0.93149
Ran for 121550 frames Cell Events: 6435 mean_circ: 0.931492
Ran for 122550 frames Cell Events: 6477 mean_circ: 0.931441
Ran for 123050 frames Cell Events: 6494 mean_circ: 0.931417
Ran for 123550 frames Cell Events: 6511 mean_circ: 0.931426
Ran for 124050 frames Cell Events: 6535 mean_circ: 0.931429
Ran for 125050 frames Cell Events: 6603 mean_circ: 0.931403
Ran for 126550 frames Cell Events: 6662 mean_circ: 0.931378
Ran for 127550 frames Cell Events: 6694 mean_circ: 0.931417
Ran for 130050 frames Cell Events: 6809 mean_circ: 0.93136
Ran for 131050 frames Cell Events: 6870 mean_circ: 0.931422
Ran for 131550 frames Cell Events: 6901 mean_circ: 0.931431
Ran for 133050 frames Cell Events: 6978 mean_circ: 0.931465
Ran for 134050 frames Cell Events: 7037 mean_circ: 0.931552
Ran for 134550 frames Cell Events: 7056 mean_circ: 0.931556
Ran for 135550 frames Cell Events: 7103 mean_circ: 0.931559
Ran for 138550 frames Cell Events: 7279 mean_circ: 0.931623
Ran for 141050 frames Cell Events: 7388 mean_circ: 0.931525
Ran for 141550 frames Cell Events: 7407 mean_circ: 0.931549
Ran for 142050 frames Cell Events: 7434 mean_circ: 0.931557
Ran for 143550 frames Cell Events: 7507 mean_circ: 0.931623
Ran for 144050 frames Cell Events: 7536 mean_circ: 0.931624
Ran for 145550 frames Cell Events: 7602 mean_circ: 0.931599
Ran for 146050 frames Cell Events: 7638 mean_circ: 0.931601
Ran for 147550 frames Cell Events: 7733 mean_circ: 0.931595
Ran for 150050 frames Cell Events: 7857 mean_circ: 0.931626
Ran for 151050 frames Cell Events: 7906 mean_circ: 0.931626
Ran for 152050 frames Cell Events: 7956 mean_circ: 0.931563
Ran for 153050 frames Cell Events: 8011 mean_circ: 0.931553
End this measurment..
Stopping time: 3.87994e+007
****************************************
Measurement finished
Mean Circularitiy: 0.931551
Mean Area: 682.763
Mean FL-1 intensity: 3870.94
Mean FL-2 intensity: 5761.03
Mean FL-3 intensity: 0
frames processed: 151168
Total Time: 76886.6ms
Number of Runs (new bg img) 0
Frames collected: 153682
Skipped frames: 111770
Rescued Frames: 5088805
Samples DAQ: 38420571
Samples written: 76840748
Samples per Channel: 38420374
FPS: 1998.81
Samples per second: 499702
Expected samples/s: 500000
Events: 8037
Events per second: 104.531
jumped back (PMT): 24
#Frames read, frequency
# 0 frames :236236
# 1 frames :58652
# 2 frames :2745
# 3 frames :1058
# 4 frames :561
# 5 frames :312
# 6 frames :1560
# 7 frames :1211
# 8 frames :959
# 9 frames :671
# 10 frames :611
# 11 frames :381
# 12 frames :316
# 13 frames :216
# 14 frames :225
# 15 frames :197
# 16 frames :155
# 17 frames :121
# 18 frames :131
# 19 frames :96
# 20 frames :83
# 21 frames :61
# 22 frames :52
# 23 frames :51
# 24 frames :39
# 25 frames :38
# 26 frames :35
# 27 frames :23
# 28 frames :33
# 29 frames :17
# 30 frames :18
# 31 frames :20
# 32 frames :26
# 33 frames :12
# 34 frames :14
# 35 frames :11
# 36 frames :11
# 37 frames :11
# 38 frames :8
# 39 frames :5
# 40 frames :10
# 41 frames :7
# 42 frames :7
# 43 frames :4
# 44 frames :4
# 45 frames :2
# 46 frames :3
# 47 frames :4
# 48 frames :6
# 49 frames :1
# 50 frames :1
# 51 frames :1
# 52 frames :2
# 54 frames :1
# 55 frames :1
# 56 frames :2
# 58 frames :1
# 59 frames :1
# 61 frames :1
# 65 frames :1
# 69 frames :1
# 75 frames :1
# 78 frames :1
# 85 frames :1
M1_para.ini
[General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100
[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0
#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 26.361
parameters.txt
#fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0