M1_20170518_1242_dsRNA_G5_col.rtdc
Click on the log titles below to expand the log entries present in this DC resource.
dclab-tdms2rtdc
{"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "b08cd6334242618a0c3979389623a0bf",
"name": "M1_20170518_1242_dsRNA_G5_col.tdms"
}
],
"libraries": {
"dclab": "0.59.1",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.6:8b6ee5b, Oct 2 2023 14:57:12",
"implementation": "CPython",
"version": "3.11.6"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-09",
"time": "13:50:42"
}
}
FG_Config.mcf
[Kamerasektion]ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;
[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;
[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini
[Framerate]Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6
[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini
[EVENT LOG] fRT-DC V1.06170206Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 26.2782
Start pulse train for BG Aquisition..
Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 2.7478e+008
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 50 frames Cell Events: 0 mean_circ: 0
Ran for 550 frames Cell Events: 2 mean_circ: 0.890844
Ran for 1050 frames Cell Events: 5 mean_circ: 0.946923
Ran for 1550 frames Cell Events: 9 mean_circ: 0.935408
Ran for 2050 frames Cell Events: 15 mean_circ: 0.939154
Ran for 2550 frames Cell Events: 19 mean_circ: 0.908486
Ran for 3050 frames Cell Events: 26 mean_circ: 0.923285
Ran for 3550 frames Cell Events: 26 mean_circ: 0.923285
Ran for 4050 frames Cell Events: 34 mean_circ: 0.927465
Ran for 4550 frames Cell Events: 36 mean_circ: 0.920762
Ran for 5050 frames Cell Events: 42 mean_circ: 0.922055
Ran for 5550 frames Cell Events: 43 mean_circ: 0.921931
Ran for 6050 frames Cell Events: 49 mean_circ: 0.924975
Ran for 6550 frames Cell Events: 51 mean_circ: 0.925172
Ran for 7050 frames Cell Events: 57 mean_circ: 0.925465
Ran for 7550 frames Cell Events: 60 mean_circ: 0.927255
Ran for 8050 frames Cell Events: 66 mean_circ: 0.928098
Ran for 8550 frames Cell Events: 69 mean_circ: 0.924637
Ran for 9050 frames Cell Events: 73 mean_circ: 0.926569
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Ran for 11550 frames Cell Events: 97 mean_circ: 0.928633
Ran for 12050 frames Cell Events: 105 mean_circ: 0.925671
Ran for 12550 frames Cell Events: 117 mean_circ: 0.923664
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Ran for 15550 frames Cell Events: 155 mean_circ: 0.926092
Ran for 16050 frames Cell Events: 159 mean_circ: 0.92673
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Ran for 20550 frames Cell Events: 216 mean_circ: 0.925509
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Ran for 22050 frames Cell Events: 239 mean_circ: 0.926313
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Ran for 23050 frames Cell Events: 256 mean_circ: 0.92532
Ran for 24050 frames Cell Events: 270 mean_circ: 0.92572
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Ran for 25550 frames Cell Events: 281 mean_circ: 0.925287
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Ran for 27050 frames Cell Events: 312 mean_circ: 0.92677
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Ran for 29550 frames Cell Events: 336 mean_circ: 0.925956
Ran for 30050 frames Cell Events: 339 mean_circ: 0.926231
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Ran for 31050 frames Cell Events: 356 mean_circ: 0.925598
Ran for 31550 frames Cell Events: 357 mean_circ: 0.925684
Ran for 32050 frames Cell Events: 360 mean_circ: 0.925414
Ran for 32550 frames Cell Events: 372 mean_circ: 0.926056
Ran for 33050 frames Cell Events: 376 mean_circ: 0.925476
Ran for 33550 frames Cell Events: 383 mean_circ: 0.925744
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Ran for 71550 frames Cell Events: 948 mean_circ: 0.929374
Ran for 72050 frames Cell Events: 954 mean_circ: 0.929475
Ran for 73550 frames Cell Events: 990 mean_circ: 0.929685
Ran for 74050 frames Cell Events: 993 mean_circ: 0.929778
Ran for 74550 frames Cell Events: 1003 mean_circ: 0.929823
Ran for 75050 frames Cell Events: 1010 mean_circ: 0.929889
Ran for 75550 frames Cell Events: 1019 mean_circ: 0.929981
Ran for 76050 frames Cell Events: 1026 mean_circ: 0.92973
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Ran for 77050 frames Cell Events: 1037 mean_circ: 0.929684
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Ran for 80050 frames Cell Events: 1085 mean_circ: 0.929056
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Ran for 82050 frames Cell Events: 1102 mean_circ: 0.929222
Ran for 82550 frames Cell Events: 1112 mean_circ: 0.929345
Ran for 83050 frames Cell Events: 1125 mean_circ: 0.928678
Ran for 83550 frames Cell Events: 1134 mean_circ: 0.928854
Ran for 84050 frames Cell Events: 1141 mean_circ: 0.929005
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Ran for 91050 frames Cell Events: 1243 mean_circ: 0.92799
Ran for 91550 frames Cell Events: 1251 mean_circ: 0.92819
Ran for 92050 frames Cell Events: 1265 mean_circ: 0.928431
Ran for 92550 frames Cell Events: 1270 mean_circ: 0.928292
Ran for 93050 frames Cell Events: 1273 mean_circ: 0.928176
Ran for 93550 frames Cell Events: 1282 mean_circ: 0.928176
Ran for 94050 frames Cell Events: 1287 mean_circ: 0.928427
Ran for 94550 frames Cell Events: 1294 mean_circ: 0.928572
Ran for 95550 frames Cell Events: 1306 mean_circ: 0.928374
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Ran for 97050 frames Cell Events: 1328 mean_circ: 0.92836
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Ran for 100550 frames Cell Events: 1392 mean_circ: 0.928614
Ran for 101050 frames Cell Events: 1400 mean_circ: 0.928873
Ran for 102050 frames Cell Events: 1415 mean_circ: 0.928936
Ran for 102550 frames Cell Events: 1426 mean_circ: 0.928855
Ran for 103050 frames Cell Events: 1433 mean_circ: 0.928594
Ran for 103550 frames Cell Events: 1441 mean_circ: 0.928765
Ran for 104050 frames Cell Events: 1450 mean_circ: 0.928733
Ran for 104550 frames Cell Events: 1457 mean_circ: 0.928613
Ran for 105550 frames Cell Events: 1475 mean_circ: 0.928549
Ran for 106050 frames Cell Events: 1485 mean_circ: 0.928745
Ran for 106550 frames Cell Events: 1492 mean_circ: 0.928895
Ran for 107050 frames Cell Events: 1502 mean_circ: 0.928566
Ran for 107550 frames Cell Events: 1506 mean_circ: 0.928659
Ran for 108550 frames Cell Events: 1521 mean_circ: 0.928405
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Ran for 110050 frames Cell Events: 1550 mean_circ: 0.928413
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Ran for 111050 frames Cell Events: 1564 mean_circ: 0.928457
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Ran for 122550 frames Cell Events: 1736 mean_circ: 0.928228
Ran for 123050 frames Cell Events: 1746 mean_circ: 0.928286
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Ran for 156550 frames Cell Events: 2214 mean_circ: 0.928413
Ran for 157050 frames Cell Events: 2223 mean_circ: 0.928366
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Ran for 161050 frames Cell Events: 2284 mean_circ: 0.928154
Ran for 161550 frames Cell Events: 2286 mean_circ: 0.928151
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Ran for 374050 frames Cell Events: 6467 mean_circ: 0.925971
Ran for 374550 frames Cell Events: 6475 mean_circ: 0.925988
Ran for 375050 frames Cell Events: 6484 mean_circ: 0.926008
Ran for 375550 frames Cell Events: 6493 mean_circ: 0.926052
Ran for 376050 frames Cell Events: 6500 mean_circ: 0.926038
Ran for 376550 frames Cell Events: 6516 mean_circ: 0.926035
Ran for 377050 frames Cell Events: 6527 mean_circ: 0.926066
Ran for 378050 frames Cell Events: 6553 mean_circ: 0.926002
Ran for 378550 frames Cell Events: 6562 mean_circ: 0.926019
Ran for 379050 frames Cell Events: 6573 mean_circ: 0.926043
Ran for 379550 frames Cell Events: 6585 mean_circ: 0.926014
Ran for 380550 frames Cell Events: 6612 mean_circ: 0.926034
Ran for 381050 frames Cell Events: 6629 mean_circ: 0.925998
Ran for 382050 frames Cell Events: 6659 mean_circ: 0.926045
Ran for 383050 frames Cell Events: 6670 mean_circ: 0.926051
Ran for 383550 frames Cell Events: 6680 mean_circ: 0.926043
Ran for 384050 frames Cell Events: 6693 mean_circ: 0.926027
Ran for 384550 frames Cell Events: 6698 mean_circ: 0.926039
Ran for 385050 frames Cell Events: 6704 mean_circ: 0.926065
Ran for 386050 frames Cell Events: 6721 mean_circ: 0.926147
Ran for 386550 frames Cell Events: 6730 mean_circ: 0.926135
Ran for 387050 frames Cell Events: 6736 mean_circ: 0.926176
Ran for 387550 frames Cell Events: 6746 mean_circ: 0.926197
Ran for 388050 frames Cell Events: 6757 mean_circ: 0.926237
Ran for 389550 frames Cell Events: 6775 mean_circ: 0.926194
Ran for 391050 frames Cell Events: 6816 mean_circ: 0.926201
Ran for 392050 frames Cell Events: 6846 mean_circ: 0.926166
Ran for 392550 frames Cell Events: 6851 mean_circ: 0.926217
Ran for 393050 frames Cell Events: 6861 mean_circ: 0.926197
Ran for 393550 frames Cell Events: 6876 mean_circ: 0.926143
Ran for 394050 frames Cell Events: 6889 mean_circ: 0.926165
Ran for 394550 frames Cell Events: 6894 mean_circ: 0.926194
Ran for 395050 frames Cell Events: 6903 mean_circ: 0.926169
Ran for 395550 frames Cell Events: 6907 mean_circ: 0.926194
Ran for 396050 frames Cell Events: 6921 mean_circ: 0.92618
Ran for 396550 frames Cell Events: 6930 mean_circ: 0.926188
Ran for 397050 frames Cell Events: 6936 mean_circ: 0.926201
Ran for 397550 frames Cell Events: 6947 mean_circ: 0.926231
Ran for 398550 frames Cell Events: 6981 mean_circ: 0.926171
Ran for 399050 frames Cell Events: 6997 mean_circ: 0.926166
Ran for 400550 frames Cell Events: 7036 mean_circ: 0.926054
Ran for 401050 frames Cell Events: 7056 mean_circ: 0.926039
Ran for 401550 frames Cell Events: 7063 mean_circ: 0.926069
Ran for 402050 frames Cell Events: 7069 mean_circ: 0.926085
Ran for 402550 frames Cell Events: 7084 mean_circ: 0.926047
Ran for 403050 frames Cell Events: 7096 mean_circ: 0.926058
Ran for 403550 frames Cell Events: 7109 mean_circ: 0.926083
Ran for 404550 frames Cell Events: 7125 mean_circ: 0.926141
Ran for 405550 frames Cell Events: 7148 mean_circ: 0.926134
Ran for 406550 frames Cell Events: 7171 mean_circ: 0.926121
Ran for 407050 frames Cell Events: 7188 mean_circ: 0.926162
Ran for 407550 frames Cell Events: 7196 mean_circ: 0.926169
Ran for 408050 frames Cell Events: 7203 mean_circ: 0.926167
Ran for 408550 frames Cell Events: 7218 mean_circ: 0.926124
Ran for 409050 frames Cell Events: 7229 mean_circ: 0.926148
Ran for 409550 frames Cell Events: 7234 mean_circ: 0.926127
Ran for 410050 frames Cell Events: 7244 mean_circ: 0.926139
Ran for 410550 frames Cell Events: 7261 mean_circ: 0.926175
Ran for 411550 frames Cell Events: 7280 mean_circ: 0.926154
Ran for 412050 frames Cell Events: 7290 mean_circ: 0.926187
Ran for 412550 frames Cell Events: 7298 mean_circ: 0.926165
Ran for 413050 frames Cell Events: 7307 mean_circ: 0.926168
Ran for 413550 frames Cell Events: 7318 mean_circ: 0.926198
Ran for 414050 frames Cell Events: 7339 mean_circ: 0.926179
Ran for 414550 frames Cell Events: 7347 mean_circ: 0.92611
Ran for 415550 frames Cell Events: 7377 mean_circ: 0.926144
Ran for 416050 frames Cell Events: 7390 mean_circ: 0.926066
Ran for 416550 frames Cell Events: 7405 mean_circ: 0.926093
Ran for 417050 frames Cell Events: 7417 mean_circ: 0.92614
Ran for 418050 frames Cell Events: 7437 mean_circ: 0.926188
Ran for 418550 frames Cell Events: 7442 mean_circ: 0.926197
Ran for 419550 frames Cell Events: 7462 mean_circ: 0.926192
Ran for 420550 frames Cell Events: 7477 mean_circ: 0.926229
Ran for 421050 frames Cell Events: 7490 mean_circ: 0.926238
Ran for 421550 frames Cell Events: 7502 mean_circ: 0.926233
Ran for 422050 frames Cell Events: 7514 mean_circ: 0.926214
Ran for 422550 frames Cell Events: 7521 mean_circ: 0.926183
Ran for 423050 frames Cell Events: 7528 mean_circ: 0.926164
Ran for 424050 frames Cell Events: 7545 mean_circ: 0.926115
Ran for 424550 frames Cell Events: 7562 mean_circ: 0.926144
Ran for 425050 frames Cell Events: 7576 mean_circ: 0.926148
Ran for 425550 frames Cell Events: 7584 mean_circ: 0.926102
Ran for 426050 frames Cell Events: 7597 mean_circ: 0.926088
Ran for 426550 frames Cell Events: 7606 mean_circ: 0.926082
Ran for 427050 frames Cell Events: 7610 mean_circ: 0.926105
Ran for 427550 frames Cell Events: 7617 mean_circ: 0.926063
Ran for 428050 frames Cell Events: 7623 mean_circ: 0.926057
Ran for 428550 frames Cell Events: 7631 mean_circ: 0.926011
Ran for 429550 frames Cell Events: 7651 mean_circ: 0.925894
Ran for 430050 frames Cell Events: 7670 mean_circ: 0.925914
Ran for 431550 frames Cell Events: 7715 mean_circ: 0.925991
Ran for 432050 frames Cell Events: 7723 mean_circ: 0.925963
Ran for 434050 frames Cell Events: 7776 mean_circ: 0.926036
Ran for 434550 frames Cell Events: 7787 mean_circ: 0.926062
Ran for 435050 frames Cell Events: 7804 mean_circ: 0.926033
Ran for 435550 frames Cell Events: 7820 mean_circ: 0.926012
Ran for 436050 frames Cell Events: 7831 mean_circ: 0.926029
Ran for 436550 frames Cell Events: 7845 mean_circ: 0.92607
Ran for 437050 frames Cell Events: 7853 mean_circ: 0.926072
Ran for 437550 frames Cell Events: 7861 mean_circ: 0.926085
Ran for 439050 frames Cell Events: 7904 mean_circ: 0.926063
Ran for 440550 frames Cell Events: 7933 mean_circ: 0.92603
Ran for 441050 frames Cell Events: 7942 mean_circ: 0.925999
Ran for 441550 frames Cell Events: 7950 mean_circ: 0.925935
Ran for 442550 frames Cell Events: 7974 mean_circ: 0.92589
Ran for 443050 frames Cell Events: 7985 mean_circ: 0.925937
Ran for 444050 frames Cell Events: 8004 mean_circ: 0.925976
Ran for 444550 frames Cell Events: 8018 mean_circ: 0.925961
Ran for 445050 frames Cell Events: 8033 mean_circ: 0.925981
Ran for 445550 frames Cell Events: 8041 mean_circ: 0.926006
Ran for 446050 frames Cell Events: 8049 mean_circ: 0.926006
Ran for 446550 frames Cell Events: 8061 mean_circ: 0.925995
Ran for 447050 frames Cell Events: 8070 mean_circ: 0.926022
Ran for 447550 frames Cell Events: 8082 mean_circ: 0.925989
End this measurment..
Stopping time: 2.75004e+008
****************************************
Measurement finished
Mean Circularitiy: 0.925986
Mean Area: 714.124
Mean FL-1 intensity: 3110.58
Mean FL-2 intensity: 4780.84
Mean FL-3 intensity: 0
frames processed: 445288
Total Time: 223979ms
Number of Runs (new bg img) 0
Frames collected: 447782
Skipped frames: 2494
Rescued Frames: 127988
Samples DAQ: 111945502
Samples written: 223890812
Samples per Channel: 111945406
FPS: 1999.21
Samples per second: 499803
Expected samples/s: 500000
Events: 8093
Events per second: 36.1328
jumped back (PMT): 6406
#Frames read, frequency
# 0 frames :578221
# 1 frames :293700
# 2 frames :6603
# 3 frames :2695
# 4 frames :1463
# 5 frames :987
# 6 frames :1126
# 7 frames :2720
# 8 frames :1792
# 9 frames :1278
# 10 frames :981
# 11 frames :758
# 12 frames :734
# 13 frames :570
# 14 frames :398
# 15 frames :340
# 16 frames :250
# 17 frames :223
# 18 frames :163
# 19 frames :114
# 20 frames :92
# 21 frames :67
# 22 frames :31
# 23 frames :41
# 24 frames :28
# 25 frames :13
# 26 frames :27
# 27 frames :19
# 28 frames :8
# 29 frames :16
# 30 frames :17
# 31 frames :2
# 32 frames :8
# 33 frames :10
# 34 frames :2
# 35 frames :5
# 36 frames :5
# 37 frames :1
# 38 frames :1
# 39 frames :4
# 40 frames :1
# 42 frames :1
# 44 frames :1
# 46 frames :2
# 48 frames :2
# 49 frames :1
# 53 frames :1
# 54 frames :1
# 59 frames :3
# 60 frames :1
# 67 frames :1
M1_para.ini
[General]Measurement Number = 1
Flow Rate [ul/s] = 0.04
Region = "Channel"
Channel width = 20
Current LED = 100
[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0
#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 26.2782
parameters.txt
#fRT-DC Configuration file# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 300
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0
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Additional Information
Field | Value |
---|---|
license | Creative Commons Public Domain Dedication |
created | 3 months ago |
etag | ac34572387d795b391ec5d6790006a8f |
format | RT-FDC |
id | 91f12627-0ca3-48af-b689-20fd2c310727 |
metadata modified | 3 months ago |
mimetype | RT-DC |
package id | a36cd1ff-1be5-44d7-8742-531472ec4564 |
position | 0 |
s3 available | True |
s3 url | https://objectstore.hpccloud.mpcdf.mpg.de/circle-8a23b5b7-6d86-46b7-9833-62373e9ec110/resource/91f/126/27-0ca3-48af-b689-20fd2c310727 |
sha256 | 56bd68f71f3e30aaeef8350e1ecb125b5677485f1d826671402ebd4704779275 |
size | 143.2 MiB |
dc:experiment:date | 2017-05-18 |
dc:experiment:event count | 8,092 |
dc:experiment:run identifier | None-42d6 |
dc:experiment:run index | 1 |
dc:experiment:sample | 20170518_1242_dsRNA_G5_col |
dc:experiment:time | 12:42:21 |
dc:fluorescence:bit depth | 16 |
dc:fluorescence:channel 1 name | FL1 |
dc:fluorescence:channel 2 name | FL2 |
dc:fluorescence:channel count | 2 |
dc:fluorescence:channels installed | 3 |
dc:fluorescence:laser 1 lambda | 488 |
dc:fluorescence:laser 1 power | 10 |
dc:fluorescence:laser 2 lambda | 561 |
dc:fluorescence:laser 2 power | 35 |
dc:fluorescence:laser 3 lambda | 640 |
dc:fluorescence:laser 3 power | 0 |
dc:fluorescence:laser count | 2 |
dc:fluorescence:lasers installed | 3 |
dc:fluorescence:sample rate | 500,000 |
dc:fluorescence:samples per event | 300 |
dc:fluorescence:signal max | 1 |
dc:fluorescence:signal min | -1 |
dc:fluorescence:trace median | 5 |
dc:imaging:flash device | LED (undefined) |
dc:imaging:flash duration | 2 |
dc:imaging:frame rate | 2,000 |
dc:imaging:pixel size | 0.34 |
dc:imaging:roi position x | 504 |
dc:imaging:roi position y | 472 |
dc:imaging:roi size x | 256 |
dc:imaging:roi size y | 96 |
dc:online contour:bin area min | 50 |
dc:online contour:bin kernel | 5 |
dc:online contour:bin threshold | 6 |
dc:online contour:image blur | 0 |
dc:online contour:no absdiff | True |
dc:setup:channel width | 20 |
dc:setup:chip region | channel |
dc:setup:flow rate | 0.04 |
dc:setup:module composition | Cell_Flow_2, Fluor |
dc:setup:software version | fRT-DC V1.06170206 | dclab 0.59.1 |
sp:cells:cell type | KC167 |
sp:cells:live | True |
sp:cells:organism | Drosophila melanogaster |
sp:chip:channel width | 20 |
sp:experiment:buffer osmolality | 325 |
sp:experiment:buffer ph | 6.8 |
sp:experiment:concentration | 5 |
sp:identifiers:labels | GFP tdTomato |
sp:identifiers:tags | NLS-GFP |