M1_20170518_1447_dsRNA_H5_col.rtdc
Click on the log titles below to expand the log entries present in this DC resource.
dclab-tdms2rtdc
{"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "daec1f1de6db59e313d2b345072a6a8f",
"name": "M1_20170518_1447_dsRNA_H5_col.tdms"
}
],
"libraries": {
"dclab": "0.59.1",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.6:8b6ee5b, Oct 2 2023 14:57:12",
"implementation": "CPython",
"version": "3.11.6"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-09",
"time": "15:37:02"
}
}
FG_Config.mcf
[Kamerasektion]ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;
[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;
[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini
[Framerate]Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6
[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini
[EVENT LOG] fRT-DC V1.06170206Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 27.1075
Start pulse train for BG Aquisition..
Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 2.82298e+008
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 50 frames Cell Events: 0 mean_circ: 0.93407
Ran for 550 frames Cell Events: 4 mean_circ: 0.930409
Ran for 1550 frames Cell Events: 14 mean_circ: 0.925956
Ran for 2050 frames Cell Events: 17 mean_circ: 0.93082
Ran for 2550 frames Cell Events: 17 mean_circ: 0.93082
Ran for 3050 frames Cell Events: 20 mean_circ: 0.929054
Ran for 3550 frames Cell Events: 25 mean_circ: 0.937205
Ran for 4050 frames Cell Events: 29 mean_circ: 0.937054
Ran for 4550 frames Cell Events: 32 mean_circ: 0.935177
Ran for 5050 frames Cell Events: 34 mean_circ: 0.936474
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Ran for 7050 frames Cell Events: 51 mean_circ: 0.935554
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Ran for 8050 frames Cell Events: 63 mean_circ: 0.935346
Ran for 8550 frames Cell Events: 69 mean_circ: 0.93475
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Ran for 10050 frames Cell Events: 81 mean_circ: 0.937449
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Ran for 11050 frames Cell Events: 93 mean_circ: 0.935433
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Ran for 12050 frames Cell Events: 109 mean_circ: 0.932225
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Ran for 26050 frames Cell Events: 253 mean_circ: 0.930314
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Ran for 70050 frames Cell Events: 644 mean_circ: 0.929368
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Ran for 71550 frames Cell Events: 657 mean_circ: 0.928876
Ran for 72550 frames Cell Events: 668 mean_circ: 0.928757
Ran for 73050 frames Cell Events: 673 mean_circ: 0.928736
Ran for 73550 frames Cell Events: 677 mean_circ: 0.928829
Ran for 74050 frames Cell Events: 680 mean_circ: 0.928899
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Ran for 81050 frames Cell Events: 762 mean_circ: 0.928738
Ran for 81550 frames Cell Events: 764 mean_circ: 0.928773
Ran for 82050 frames Cell Events: 771 mean_circ: 0.928716
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Ran for 83550 frames Cell Events: 792 mean_circ: 0.928497
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Ran for 91050 frames Cell Events: 885 mean_circ: 0.928028
Ran for 91550 frames Cell Events: 887 mean_circ: 0.927999
Ran for 92050 frames Cell Events: 893 mean_circ: 0.927823
Ran for 92550 frames Cell Events: 898 mean_circ: 0.927915
Ran for 93050 frames Cell Events: 905 mean_circ: 0.927943
Ran for 93550 frames Cell Events: 913 mean_circ: 0.928162
Ran for 94050 frames Cell Events: 920 mean_circ: 0.928099
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Ran for 100050 frames Cell Events: 966 mean_circ: 0.928368
Ran for 100550 frames Cell Events: 969 mean_circ: 0.928374
Ran for 101050 frames Cell Events: 972 mean_circ: 0.928332
Ran for 101550 frames Cell Events: 974 mean_circ: 0.928367
Ran for 102050 frames Cell Events: 977 mean_circ: 0.928419
Ran for 102550 frames Cell Events: 980 mean_circ: 0.928606
Ran for 103050 frames Cell Events: 987 mean_circ: 0.928689
Ran for 103550 frames Cell Events: 996 mean_circ: 0.928839
Ran for 104050 frames Cell Events: 1003 mean_circ: 0.928782
Ran for 104550 frames Cell Events: 1005 mean_circ: 0.928769
Ran for 105050 frames Cell Events: 1012 mean_circ: 0.928758
Ran for 105550 frames Cell Events: 1017 mean_circ: 0.928773
Ran for 106050 frames Cell Events: 1017 mean_circ: 0.928773
Ran for 106550 frames Cell Events: 1019 mean_circ: 0.928712
Ran for 107050 frames Cell Events: 1023 mean_circ: 0.928599
Ran for 108050 frames Cell Events: 1033 mean_circ: 0.928622
Ran for 108550 frames Cell Events: 1040 mean_circ: 0.92867
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Ran for 111050 frames Cell Events: 1064 mean_circ: 0.928448
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Ran for 122550 frames Cell Events: 1164 mean_circ: 0.927851
Ran for 123050 frames Cell Events: 1165 mean_circ: 0.927876
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Ran for 142550 frames Cell Events: 1354 mean_circ: 0.928327
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Ran for 148550 frames Cell Events: 1410 mean_circ: 0.927626
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Ran for 151050 frames Cell Events: 1433 mean_circ: 0.927735
Ran for 151550 frames Cell Events: 1442 mean_circ: 0.927876
Ran for 152050 frames Cell Events: 1451 mean_circ: 0.928127
Ran for 153050 frames Cell Events: 1461 mean_circ: 0.928
Ran for 154050 frames Cell Events: 1472 mean_circ: 0.928179
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Ran for 740050 frames Cell Events: 7600 mean_circ: 0.929833
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Ran for 751050 frames Cell Events: 7704 mean_circ: 0.929872
Ran for 751550 frames Cell Events: 7706 mean_circ: 0.929864
Ran for 752050 frames Cell Events: 7712 mean_circ: 0.929872
Ran for 753050 frames Cell Events: 7723 mean_circ: 0.929881
Ran for 753550 frames Cell Events: 7732 mean_circ: 0.929872
Ran for 754050 frames Cell Events: 7741 mean_circ: 0.929888
Ran for 754550 frames Cell Events: 7746 mean_circ: 0.929898
Ran for 755050 frames Cell Events: 7752 mean_circ: 0.929905
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Ran for 757050 frames Cell Events: 7774 mean_circ: 0.929923
Ran for 757550 frames Cell Events: 7781 mean_circ: 0.929919
Ran for 759050 frames Cell Events: 7796 mean_circ: 0.929945
Ran for 759550 frames Cell Events: 7801 mean_circ: 0.929933
Ran for 760050 frames Cell Events: 7805 mean_circ: 0.929936
Ran for 760550 frames Cell Events: 7808 mean_circ: 0.929938
Ran for 761050 frames Cell Events: 7817 mean_circ: 0.929946
Ran for 761550 frames Cell Events: 7828 mean_circ: 0.929915
Ran for 762050 frames Cell Events: 7830 mean_circ: 0.929894
Ran for 762550 frames Cell Events: 7837 mean_circ: 0.929868
Ran for 763050 frames Cell Events: 7846 mean_circ: 0.929852
Ran for 763550 frames Cell Events: 7848 mean_circ: 0.929872
Ran for 764050 frames Cell Events: 7852 mean_circ: 0.929864
Ran for 764550 frames Cell Events: 7858 mean_circ: 0.92982
Ran for 765050 frames Cell Events: 7861 mean_circ: 0.929831
Ran for 765550 frames Cell Events: 7864 mean_circ: 0.92984
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Ran for 766550 frames Cell Events: 7878 mean_circ: 0.929825
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Ran for 767550 frames Cell Events: 7883 mean_circ: 0.929824
Ran for 768050 frames Cell Events: 7887 mean_circ: 0.929816
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Ran for 772550 frames Cell Events: 7930 mean_circ: 0.929871
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Ran for 775050 frames Cell Events: 7962 mean_circ: 0.929905
Ran for 775550 frames Cell Events: 7975 mean_circ: 0.929915
Ran for 776050 frames Cell Events: 7979 mean_circ: 0.929918
Ran for 776550 frames Cell Events: 7984 mean_circ: 0.92992
Ran for 777050 frames Cell Events: 7994 mean_circ: 0.929917
Ran for 777550 frames Cell Events: 7998 mean_circ: 0.929911
Ran for 778050 frames Cell Events: 8003 mean_circ: 0.929922
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Ran for 782050 frames Cell Events: 8050 mean_circ: 0.929965
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Ran for 783050 frames Cell Events: 8058 mean_circ: 0.929958
Ran for 783550 frames Cell Events: 8063 mean_circ: 0.929962
Ran for 784050 frames Cell Events: 8066 mean_circ: 0.929965
Ran for 784550 frames Cell Events: 8072 mean_circ: 0.929948
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Ran for 789550 frames Cell Events: 8127 mean_circ: 0.929909
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Ran for 792050 frames Cell Events: 8155 mean_circ: 0.929842
Ran for 792550 frames Cell Events: 8165 mean_circ: 0.929874
Ran for 793050 frames Cell Events: 8170 mean_circ: 0.92988
Ran for 793550 frames Cell Events: 8173 mean_circ: 0.929897
Ran for 794050 frames Cell Events: 8177 mean_circ: 0.929892
Ran for 794550 frames Cell Events: 8184 mean_circ: 0.929899
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Ran for 795550 frames Cell Events: 8198 mean_circ: 0.929899
Ran for 796050 frames Cell Events: 8207 mean_circ: 0.929879
Ran for 796550 frames Cell Events: 8213 mean_circ: 0.929882
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Ran for 800550 frames Cell Events: 8259 mean_circ: 0.929894
Ran for 801050 frames Cell Events: 8264 mean_circ: 0.929902
Ran for 801550 frames Cell Events: 8269 mean_circ: 0.929897
Ran for 802550 frames Cell Events: 8278 mean_circ: 0.929932
Ran for 803050 frames Cell Events: 8282 mean_circ: 0.929932
Ran for 803550 frames Cell Events: 8285 mean_circ: 0.929933
Ran for 804050 frames Cell Events: 8287 mean_circ: 0.929937
Ran for 804550 frames Cell Events: 8294 mean_circ: 0.929925
Ran for 805050 frames Cell Events: 8301 mean_circ: 0.929915
Ran for 805550 frames Cell Events: 8304 mean_circ: 0.929913
Ran for 806050 frames Cell Events: 8306 mean_circ: 0.929917
Ran for 806550 frames Cell Events: 8313 mean_circ: 0.92993
Ran for 807050 frames Cell Events: 8319 mean_circ: 0.929943
Ran for 808050 frames Cell Events: 8327 mean_circ: 0.929969
Ran for 808550 frames Cell Events: 8333 mean_circ: 0.929965
Ran for 809050 frames Cell Events: 8335 mean_circ: 0.929969
Ran for 809550 frames Cell Events: 8339 mean_circ: 0.929975
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Ran for 810550 frames Cell Events: 8351 mean_circ: 0.929958
Ran for 811050 frames Cell Events: 8356 mean_circ: 0.929963
Ran for 811550 frames Cell Events: 8359 mean_circ: 0.929959
Ran for 812050 frames Cell Events: 8362 mean_circ: 0.929948
Ran for 812550 frames Cell Events: 8370 mean_circ: 0.929969
Ran for 813550 frames Cell Events: 8378 mean_circ: 0.929979
Ran for 814050 frames Cell Events: 8386 mean_circ: 0.929975
Ran for 814550 frames Cell Events: 8392 mean_circ: 0.929937
Ran for 815050 frames Cell Events: 8394 mean_circ: 0.929939
Ran for 815550 frames Cell Events: 8396 mean_circ: 0.929948
Ran for 816050 frames Cell Events: 8399 mean_circ: 0.929951
Ran for 816550 frames Cell Events: 8405 mean_circ: 0.929945
Ran for 817050 frames Cell Events: 8410 mean_circ: 0.929967
Ran for 817550 frames Cell Events: 8412 mean_circ: 0.929969
Ran for 818550 frames Cell Events: 8431 mean_circ: 0.929984
Ran for 819050 frames Cell Events: 8436 mean_circ: 0.929974
Ran for 819550 frames Cell Events: 8441 mean_circ: 0.929931
Ran for 820050 frames Cell Events: 8445 mean_circ: 0.929931
Ran for 820550 frames Cell Events: 8447 mean_circ: 0.929937
Ran for 821050 frames Cell Events: 8453 mean_circ: 0.929959
Ran for 821550 frames Cell Events: 8460 mean_circ: 0.929923
Ran for 822050 frames Cell Events: 8467 mean_circ: 0.929921
Ran for 822550 frames Cell Events: 8473 mean_circ: 0.929909
Ran for 823550 frames Cell Events: 8483 mean_circ: 0.9299
Ran for 824050 frames Cell Events: 8489 mean_circ: 0.929907
Ran for 824550 frames Cell Events: 8493 mean_circ: 0.929908
Ran for 825050 frames Cell Events: 8498 mean_circ: 0.929891
Ran for 825550 frames Cell Events: 8503 mean_circ: 0.929901
Ran for 826050 frames Cell Events: 8509 mean_circ: 0.929905
Ran for 827050 frames Cell Events: 8521 mean_circ: 0.929913
Ran for 828050 frames Cell Events: 8530 mean_circ: 0.929931
Ran for 828550 frames Cell Events: 8530 mean_circ: 0.929931
Ran for 829050 frames Cell Events: 8534 mean_circ: 0.929924
Ran for 829550 frames Cell Events: 8538 mean_circ: 0.929929
Ran for 830050 frames Cell Events: 8547 mean_circ: 0.929949
Ran for 830550 frames Cell Events: 8550 mean_circ: 0.929951
Ran for 831050 frames Cell Events: 8556 mean_circ: 0.929959
Ran for 831550 frames Cell Events: 8559 mean_circ: 0.929956
Ran for 832050 frames Cell Events: 8563 mean_circ: 0.929974
Ran for 833050 frames Cell Events: 8573 mean_circ: 0.929974
Ran for 833550 frames Cell Events: 8580 mean_circ: 0.92999
Ran for 834050 frames Cell Events: 8585 mean_circ: 0.929982
Ran for 834550 frames Cell Events: 8592 mean_circ: 0.929982
Ran for 835050 frames Cell Events: 8595 mean_circ: 0.929986
Ran for 835550 frames Cell Events: 8603 mean_circ: 0.929982
Ran for 836050 frames Cell Events: 8613 mean_circ: 0.930003
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Ran for 837050 frames Cell Events: 8621 mean_circ: 0.930029
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Ran for 841050 frames Cell Events: 8670 mean_circ: 0.93002
Ran for 841550 frames Cell Events: 8675 mean_circ: 0.930018
Ran for 842050 frames Cell Events: 8675 mean_circ: 0.930018
Ran for 842550 frames Cell Events: 8679 mean_circ: 0.930013
Ran for 843050 frames Cell Events: 8683 mean_circ: 0.930022
Ran for 844050 frames Cell Events: 8687 mean_circ: 0.930022
Ran for 844550 frames Cell Events: 8691 mean_circ: 0.930023
Ran for 845050 frames Cell Events: 8692 mean_circ: 0.930005
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Ran for 846050 frames Cell Events: 8704 mean_circ: 0.930003
Ran for 846550 frames Cell Events: 8710 mean_circ: 0.930009
Ran for 847050 frames Cell Events: 8715 mean_circ: 0.930017
Ran for 847550 frames Cell Events: 8720 mean_circ: 0.930022
Ran for 848050 frames Cell Events: 8726 mean_circ: 0.930039
Ran for 848550 frames Cell Events: 8730 mean_circ: 0.930046
Ran for 849050 frames Cell Events: 8735 mean_circ: 0.930046
Ran for 849550 frames Cell Events: 8743 mean_circ: 0.930055
Ran for 850550 frames Cell Events: 8767 mean_circ: 0.930064
Ran for 851050 frames Cell Events: 8772 mean_circ: 0.930064
Ran for 851550 frames Cell Events: 8777 mean_circ: 0.930062
Ran for 852050 frames Cell Events: 8778 mean_circ: 0.93007
Ran for 852550 frames Cell Events: 8784 mean_circ: 0.930082
Ran for 853050 frames Cell Events: 8785 mean_circ: 0.930075
Ran for 853550 frames Cell Events: 8791 mean_circ: 0.930096
Ran for 854050 frames Cell Events: 8795 mean_circ: 0.930091
Ran for 854550 frames Cell Events: 8800 mean_circ: 0.930102
Ran for 855050 frames Cell Events: 8805 mean_circ: 0.930109
Ran for 855550 frames Cell Events: 8810 mean_circ: 0.930075
Ran for 856050 frames Cell Events: 8815 mean_circ: 0.930069
Ran for 856550 frames Cell Events: 8822 mean_circ: 0.930065
Ran for 857050 frames Cell Events: 8828 mean_circ: 0.930081
Ran for 857550 frames Cell Events: 8830 mean_circ: 0.930078
Ran for 858050 frames Cell Events: 8835 mean_circ: 0.930078
Ran for 858550 frames Cell Events: 8838 mean_circ: 0.930086
Ran for 859050 frames Cell Events: 8845 mean_circ: 0.930091
Ran for 859550 frames Cell Events: 8850 mean_circ: 0.930107
Ran for 861050 frames Cell Events: 8870 mean_circ: 0.930101
Ran for 862050 frames Cell Events: 8874 mean_circ: 0.930108
Ran for 862550 frames Cell Events: 8877 mean_circ: 0.93011
Ran for 863050 frames Cell Events: 8881 mean_circ: 0.930118
Ran for 863550 frames Cell Events: 8889 mean_circ: 0.930114
Ran for 864050 frames Cell Events: 8891 mean_circ: 0.930122
Ran for 864550 frames Cell Events: 8894 mean_circ: 0.930127
Ran for 865050 frames Cell Events: 8899 mean_circ: 0.930128
Ran for 865550 frames Cell Events: 8907 mean_circ: 0.930118
Ran for 866050 frames Cell Events: 8914 mean_circ: 0.930132
End this measurment..
Stopping time: 2.82731e+008
****************************************
Measurement finished
Mean Circularitiy: 0.930134
Mean Area: 1035.43
Mean FL-1 intensity: 5380.79
Mean FL-2 intensity: 7592.84
Mean FL-3 intensity: 0
frames processed: 864828
Total Time: 433129ms
Number of Runs (new bg img) 0
Frames collected: 866061
Skipped frames: 31302
Rescued Frames: 1925457
Samples DAQ: 216515367
Samples written: 433030530
Samples per Channel: 216515265
FPS: 1999.55
Samples per second: 499887
Expected samples/s: 500000
Events: 8914
Events per second: 20.5805
jumped back (PMT): 8457
#Frames read, frequency
# 0 frames :1065163
# 1 frames :629644
# 2 frames :10646
# 3 frames :4801
# 4 frames :2601
# 5 frames :1928
# 6 frames :1466
# 7 frames :3641
# 8 frames :2315
# 9 frames :1553
# 10 frames :921
# 11 frames :1438
# 12 frames :1498
# 13 frames :1368
# 14 frames :947
# 15 frames :551
# 16 frames :370
# 17 frames :271
# 18 frames :232
# 19 frames :222
# 20 frames :187
# 21 frames :123
# 22 frames :61
# 23 frames :37
# 24 frames :27
# 25 frames :13
# 26 frames :16
# 27 frames :12
# 28 frames :9
# 29 frames :5
# 30 frames :5
# 31 frames :4
# 32 frames :2
# 33 frames :2
# 34 frames :4
# 35 frames :2
# 38 frames :1
# 39 frames :3
# 42 frames :1
M1_para.ini
[General]Measurement Number = 1
Flow Rate [ul/s] = 0.04
Region = "Channel"
Channel width = 20
Current LED = 100
[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0
#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 27.1075
parameters.txt
#fRT-DC Configuration file# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 300
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0
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Additional Information
Field | Value |
---|---|
license | Creative Commons Public Domain Dedication |
created | 3 months ago |
etag | 1fb5e8253d779c121258188feac1992d |
format | RT-FDC |
id | 1cb54fe4-8d7d-455f-8a21-f74b0e74b8f5 |
metadata modified | 3 months ago |
mimetype | RT-DC |
package id | f699b933-43e4-4182-9ede-8ad1e3840e75 |
position | 0 |
s3 available | True |
s3 url | https://objectstore.hpccloud.mpcdf.mpg.de/circle-8a23b5b7-6d86-46b7-9833-62373e9ec110/resource/1cb/54f/e4-8d7d-455f-8a21-f74b0e74b8f5 |
sha256 | 1ee0f1b75a2ba346463c1b00b45ae7abef98e2311ba2c9b36db1de2ab160161b |
size | 159.6 MiB |
dc:experiment:date | 2017-05-18 |
dc:experiment:event count | 8,913 |
dc:experiment:run identifier | None-8168 |
dc:experiment:run index | 1 |
dc:experiment:sample | 20170518_1447_dsRNA_H5_col |
dc:experiment:time | 14:47:39 |
dc:fluorescence:bit depth | 16 |
dc:fluorescence:channel 1 name | FL1 |
dc:fluorescence:channel 2 name | FL2 |
dc:fluorescence:channel count | 2 |
dc:fluorescence:channels installed | 3 |
dc:fluorescence:laser 1 lambda | 488 |
dc:fluorescence:laser 1 power | 10 |
dc:fluorescence:laser 2 lambda | 561 |
dc:fluorescence:laser 2 power | 35 |
dc:fluorescence:laser 3 lambda | 640 |
dc:fluorescence:laser 3 power | 0 |
dc:fluorescence:laser count | 2 |
dc:fluorescence:lasers installed | 3 |
dc:fluorescence:sample rate | 500,000 |
dc:fluorescence:samples per event | 300 |
dc:fluorescence:signal max | 1 |
dc:fluorescence:signal min | -1 |
dc:fluorescence:trace median | 5 |
dc:imaging:flash device | LED (undefined) |
dc:imaging:flash duration | 2 |
dc:imaging:frame rate | 2,000 |
dc:imaging:pixel size | 0.34 |
dc:imaging:roi position x | 504 |
dc:imaging:roi position y | 472 |
dc:imaging:roi size x | 256 |
dc:imaging:roi size y | 96 |
dc:online contour:bin area min | 50 |
dc:online contour:bin kernel | 5 |
dc:online contour:bin threshold | 6 |
dc:online contour:image blur | 0 |
dc:online contour:no absdiff | True |
dc:setup:channel width | 20 |
dc:setup:chip region | channel |
dc:setup:flow rate | 0.04 |
dc:setup:module composition | Cell_Flow_2, Fluor |
dc:setup:software version | fRT-DC V1.06170206 | dclab 0.59.1 |
sp:cells:cell type | KC167 |
sp:cells:live | True |
sp:cells:organism | Drosophila melanogaster |
sp:chip:channel width | 20 |
sp:experiment:buffer osmolality | 325 |
sp:experiment:buffer ph | 6.8 |
sp:experiment:concentration | 5 |
sp:identifiers:labels | GFP tdTomato |
sp:identifiers:tags | NLS-GFP |