M1_20170518_1456_dsRNA_H5_faster.rtdc
Click on the log titles below to expand the log entries present in this DC resource.
dclab-tdms2rtdc
{"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "284b87a24630d17c527cdb7b7ffd3427",
"name": "M1_20170518_1456_dsRNA_H5_faster.tdms"
}
],
"libraries": {
"dclab": "0.59.1",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.6:8b6ee5b, Oct 2 2023 14:57:12",
"implementation": "CPython",
"version": "3.11.6"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-09",
"time": "15:37:23"
}
}
dclab-tdms2rtdc-warnings
LimitingExportSizeWarningin dclab.rtdc_dataset.export line 303:
Not all features have the same length! Limiting output event count to 7022 (max 7023) in '7022'.
FG_Config.mcf
[Kamerasektion]ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;
[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;
[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini
[Framerate]Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6
[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini
[EVENT LOG] fRT-DC V1.06170206Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 27.1423
Start pulse train for BG Aquisition..
Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 2.82826e+008
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 50 frames Cell Events: 1 mean_circ: 0.930132
Ran for 550 frames Cell Events: 7 mean_circ: 0.910287
Ran for 1050 frames Cell Events: 12 mean_circ: 0.906838
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Ran for 565550 frames Cell Events: 5949 mean_circ: 0.924042
Ran for 566050 frames Cell Events: 5955 mean_circ: 0.924004
Ran for 566550 frames Cell Events: 5964 mean_circ: 0.924055
Ran for 567050 frames Cell Events: 5969 mean_circ: 0.924053
Ran for 567550 frames Cell Events: 5977 mean_circ: 0.924018
Ran for 568050 frames Cell Events: 5980 mean_circ: 0.924021
Ran for 568550 frames Cell Events: 5982 mean_circ: 0.924
Ran for 569050 frames Cell Events: 5987 mean_circ: 0.924016
Ran for 569550 frames Cell Events: 5993 mean_circ: 0.924046
Ran for 570050 frames Cell Events: 5998 mean_circ: 0.924038
Ran for 570550 frames Cell Events: 6002 mean_circ: 0.923975
Ran for 571050 frames Cell Events: 6006 mean_circ: 0.923989
Ran for 571550 frames Cell Events: 6010 mean_circ: 0.923997
Ran for 572050 frames Cell Events: 6012 mean_circ: 0.923996
Ran for 572550 frames Cell Events: 6018 mean_circ: 0.923992
Ran for 573050 frames Cell Events: 6022 mean_circ: 0.923984
Ran for 573550 frames Cell Events: 6024 mean_circ: 0.923976
Ran for 574050 frames Cell Events: 6028 mean_circ: 0.923981
Ran for 574550 frames Cell Events: 6032 mean_circ: 0.923988
Ran for 575050 frames Cell Events: 6036 mean_circ: 0.924011
Ran for 576050 frames Cell Events: 6045 mean_circ: 0.92394
Ran for 577050 frames Cell Events: 6053 mean_circ: 0.923968
Ran for 577550 frames Cell Events: 6056 mean_circ: 0.923947
Ran for 578550 frames Cell Events: 6062 mean_circ: 0.923968
Ran for 579050 frames Cell Events: 6066 mean_circ: 0.923971
Ran for 580050 frames Cell Events: 6074 mean_circ: 0.923957
Ran for 580550 frames Cell Events: 6082 mean_circ: 0.92398
Ran for 581050 frames Cell Events: 6088 mean_circ: 0.923977
Ran for 581550 frames Cell Events: 6101 mean_circ: 0.92399
Ran for 582050 frames Cell Events: 6104 mean_circ: 0.924008
Ran for 582550 frames Cell Events: 6110 mean_circ: 0.924005
Ran for 583050 frames Cell Events: 6115 mean_circ: 0.924019
Ran for 583550 frames Cell Events: 6119 mean_circ: 0.924011
Ran for 584050 frames Cell Events: 6126 mean_circ: 0.923966
Ran for 584550 frames Cell Events: 6131 mean_circ: 0.923952
Ran for 585050 frames Cell Events: 6136 mean_circ: 0.923974
Ran for 586050 frames Cell Events: 6145 mean_circ: 0.923982
Ran for 586550 frames Cell Events: 6151 mean_circ: 0.923994
Ran for 587050 frames Cell Events: 6153 mean_circ: 0.924006
Ran for 587550 frames Cell Events: 6160 mean_circ: 0.924008
Ran for 588050 frames Cell Events: 6163 mean_circ: 0.923972
Ran for 588550 frames Cell Events: 6169 mean_circ: 0.923943
Ran for 590050 frames Cell Events: 6184 mean_circ: 0.923941
Ran for 590550 frames Cell Events: 6188 mean_circ: 0.92394
Ran for 591050 frames Cell Events: 6194 mean_circ: 0.923936
Ran for 591550 frames Cell Events: 6194 mean_circ: 0.923936
Ran for 592050 frames Cell Events: 6201 mean_circ: 0.923939
Ran for 592550 frames Cell Events: 6206 mean_circ: 0.923934
Ran for 593050 frames Cell Events: 6210 mean_circ: 0.923945
Ran for 594050 frames Cell Events: 6220 mean_circ: 0.923963
Ran for 595050 frames Cell Events: 6232 mean_circ: 0.924021
Ran for 595550 frames Cell Events: 6237 mean_circ: 0.924037
Ran for 596050 frames Cell Events: 6240 mean_circ: 0.924042
Ran for 596550 frames Cell Events: 6242 mean_circ: 0.92406
Ran for 597050 frames Cell Events: 6244 mean_circ: 0.924059
Ran for 597550 frames Cell Events: 6249 mean_circ: 0.924051
Ran for 598050 frames Cell Events: 6250 mean_circ: 0.924068
Ran for 598550 frames Cell Events: 6255 mean_circ: 0.924082
Ran for 599550 frames Cell Events: 6264 mean_circ: 0.924113
Ran for 600550 frames Cell Events: 6279 mean_circ: 0.924112
Ran for 601050 frames Cell Events: 6289 mean_circ: 0.924115
Ran for 601550 frames Cell Events: 6298 mean_circ: 0.924112
Ran for 603050 frames Cell Events: 6316 mean_circ: 0.924153
Ran for 604050 frames Cell Events: 6332 mean_circ: 0.924157
Ran for 605050 frames Cell Events: 6347 mean_circ: 0.924207
Ran for 605550 frames Cell Events: 6360 mean_circ: 0.924201
Ran for 606050 frames Cell Events: 6367 mean_circ: 0.924177
Ran for 606550 frames Cell Events: 6375 mean_circ: 0.924213
Ran for 607050 frames Cell Events: 6378 mean_circ: 0.924219
Ran for 607550 frames Cell Events: 6381 mean_circ: 0.92422
Ran for 608050 frames Cell Events: 6388 mean_circ: 0.924232
Ran for 608550 frames Cell Events: 6392 mean_circ: 0.924246
Ran for 609050 frames Cell Events: 6397 mean_circ: 0.924247
Ran for 609550 frames Cell Events: 6400 mean_circ: 0.924247
Ran for 610050 frames Cell Events: 6404 mean_circ: 0.924265
Ran for 610550 frames Cell Events: 6407 mean_circ: 0.924239
Ran for 611550 frames Cell Events: 6417 mean_circ: 0.924227
Ran for 612050 frames Cell Events: 6422 mean_circ: 0.924236
Ran for 612550 frames Cell Events: 6424 mean_circ: 0.924253
Ran for 613050 frames Cell Events: 6431 mean_circ: 0.924261
Ran for 613550 frames Cell Events: 6433 mean_circ: 0.924263
Ran for 614550 frames Cell Events: 6442 mean_circ: 0.924292
Ran for 615050 frames Cell Events: 6445 mean_circ: 0.924292
Ran for 616550 frames Cell Events: 6459 mean_circ: 0.92427
Ran for 617050 frames Cell Events: 6467 mean_circ: 0.92429
Ran for 617550 frames Cell Events: 6478 mean_circ: 0.924275
Ran for 618050 frames Cell Events: 6486 mean_circ: 0.924274
Ran for 618550 frames Cell Events: 6488 mean_circ: 0.924269
Ran for 619050 frames Cell Events: 6493 mean_circ: 0.924279
Ran for 619550 frames Cell Events: 6500 mean_circ: 0.924282
Ran for 620050 frames Cell Events: 6501 mean_circ: 0.924291
Ran for 620550 frames Cell Events: 6504 mean_circ: 0.924282
Ran for 621050 frames Cell Events: 6509 mean_circ: 0.924264
Ran for 621550 frames Cell Events: 6515 mean_circ: 0.924265
Ran for 622050 frames Cell Events: 6522 mean_circ: 0.924274
Ran for 622550 frames Cell Events: 6528 mean_circ: 0.924271
Ran for 623050 frames Cell Events: 6533 mean_circ: 0.924261
Ran for 623550 frames Cell Events: 6537 mean_circ: 0.924241
Ran for 624050 frames Cell Events: 6544 mean_circ: 0.924254
Ran for 624550 frames Cell Events: 6546 mean_circ: 0.924261
Ran for 625050 frames Cell Events: 6547 mean_circ: 0.924261
Ran for 625550 frames Cell Events: 6550 mean_circ: 0.924246
Ran for 626050 frames Cell Events: 6553 mean_circ: 0.924249
Ran for 626550 frames Cell Events: 6557 mean_circ: 0.924225
Ran for 627550 frames Cell Events: 6567 mean_circ: 0.924249
Ran for 628050 frames Cell Events: 6574 mean_circ: 0.92426
Ran for 629050 frames Cell Events: 6582 mean_circ: 0.924274
Ran for 629550 frames Cell Events: 6587 mean_circ: 0.924288
Ran for 630050 frames Cell Events: 6597 mean_circ: 0.924271
Ran for 630550 frames Cell Events: 6600 mean_circ: 0.924257
Ran for 631050 frames Cell Events: 6603 mean_circ: 0.924262
Ran for 631550 frames Cell Events: 6613 mean_circ: 0.924263
Ran for 632050 frames Cell Events: 6617 mean_circ: 0.924268
Ran for 632550 frames Cell Events: 6620 mean_circ: 0.924253
Ran for 633050 frames Cell Events: 6625 mean_circ: 0.924257
Ran for 633550 frames Cell Events: 6628 mean_circ: 0.924231
Ran for 634050 frames Cell Events: 6632 mean_circ: 0.924242
Ran for 634550 frames Cell Events: 6637 mean_circ: 0.92426
Ran for 635050 frames Cell Events: 6642 mean_circ: 0.924257
Ran for 635550 frames Cell Events: 6646 mean_circ: 0.924231
Ran for 636050 frames Cell Events: 6656 mean_circ: 0.924196
Ran for 636550 frames Cell Events: 6667 mean_circ: 0.924197
Ran for 637050 frames Cell Events: 6671 mean_circ: 0.924203
Ran for 637550 frames Cell Events: 6678 mean_circ: 0.924197
Ran for 638050 frames Cell Events: 6682 mean_circ: 0.924178
Ran for 638550 frames Cell Events: 6686 mean_circ: 0.924156
Ran for 639050 frames Cell Events: 6689 mean_circ: 0.924172
Ran for 639550 frames Cell Events: 6695 mean_circ: 0.924181
Ran for 640050 frames Cell Events: 6699 mean_circ: 0.924165
Ran for 640550 frames Cell Events: 6705 mean_circ: 0.924156
Ran for 641050 frames Cell Events: 6715 mean_circ: 0.924128
Ran for 641550 frames Cell Events: 6717 mean_circ: 0.924085
Ran for 642050 frames Cell Events: 6723 mean_circ: 0.92408
Ran for 642550 frames Cell Events: 6728 mean_circ: 0.924103
Ran for 643050 frames Cell Events: 6734 mean_circ: 0.924146
Ran for 643550 frames Cell Events: 6739 mean_circ: 0.924119
Ran for 644050 frames Cell Events: 6743 mean_circ: 0.92412
Ran for 644550 frames Cell Events: 6751 mean_circ: 0.924083
Ran for 645050 frames Cell Events: 6754 mean_circ: 0.92409
Ran for 645550 frames Cell Events: 6757 mean_circ: 0.924094
Ran for 646050 frames Cell Events: 6769 mean_circ: 0.924081
Ran for 647550 frames Cell Events: 6782 mean_circ: 0.924073
Ran for 648550 frames Cell Events: 6796 mean_circ: 0.924067
Ran for 649050 frames Cell Events: 6804 mean_circ: 0.924023
Ran for 649550 frames Cell Events: 6808 mean_circ: 0.92403
Ran for 650050 frames Cell Events: 6812 mean_circ: 0.924039
Ran for 650550 frames Cell Events: 6817 mean_circ: 0.924037
Ran for 651050 frames Cell Events: 6818 mean_circ: 0.924033
Ran for 651550 frames Cell Events: 6823 mean_circ: 0.924036
Ran for 652050 frames Cell Events: 6824 mean_circ: 0.924035
Ran for 652550 frames Cell Events: 6831 mean_circ: 0.924017
Ran for 653050 frames Cell Events: 6835 mean_circ: 0.924021
Ran for 653550 frames Cell Events: 6839 mean_circ: 0.924032
Ran for 654050 frames Cell Events: 6842 mean_circ: 0.924053
Ran for 654550 frames Cell Events: 6850 mean_circ: 0.92406
Ran for 655050 frames Cell Events: 6852 mean_circ: 0.924081
Ran for 655550 frames Cell Events: 6855 mean_circ: 0.924084
Ran for 656050 frames Cell Events: 6861 mean_circ: 0.924089
Ran for 656550 frames Cell Events: 6869 mean_circ: 0.9241
Ran for 657050 frames Cell Events: 6873 mean_circ: 0.924071
Ran for 658050 frames Cell Events: 6880 mean_circ: 0.92408
Ran for 658550 frames Cell Events: 6884 mean_circ: 0.924092
Ran for 659050 frames Cell Events: 6887 mean_circ: 0.924106
Ran for 659550 frames Cell Events: 6894 mean_circ: 0.924108
Ran for 660550 frames Cell Events: 6905 mean_circ: 0.924069
Ran for 661050 frames Cell Events: 6908 mean_circ: 0.924065
Ran for 661550 frames Cell Events: 6912 mean_circ: 0.924054
Ran for 662050 frames Cell Events: 6917 mean_circ: 0.924073
Ran for 662550 frames Cell Events: 6921 mean_circ: 0.924088
Ran for 663050 frames Cell Events: 6931 mean_circ: 0.924089
Ran for 664050 frames Cell Events: 6940 mean_circ: 0.924073
Ran for 664550 frames Cell Events: 6944 mean_circ: 0.92409
Ran for 665050 frames Cell Events: 6947 mean_circ: 0.92409
Ran for 665550 frames Cell Events: 6952 mean_circ: 0.924099
Ran for 666050 frames Cell Events: 6956 mean_circ: 0.924111
Ran for 666550 frames Cell Events: 6961 mean_circ: 0.924079
Ran for 667050 frames Cell Events: 6963 mean_circ: 0.924089
Ran for 667550 frames Cell Events: 6966 mean_circ: 0.92411
Ran for 668050 frames Cell Events: 6971 mean_circ: 0.924088
Ran for 668550 frames Cell Events: 6977 mean_circ: 0.924099
Ran for 669050 frames Cell Events: 6978 mean_circ: 0.924099
Ran for 669550 frames Cell Events: 6982 mean_circ: 0.924091
Ran for 670050 frames Cell Events: 6989 mean_circ: 0.924098
Ran for 670550 frames Cell Events: 6996 mean_circ: 0.924076
Ran for 671050 frames Cell Events: 7003 mean_circ: 0.924048
Ran for 671550 frames Cell Events: 7008 mean_circ: 0.924067
Ran for 672050 frames Cell Events: 7011 mean_circ: 0.924044
Ran for 672550 frames Cell Events: 7014 mean_circ: 0.924054
Ran for 673050 frames Cell Events: 7017 mean_circ: 0.924052
End this measurment..
Stopping time: 2.83163e+008
****************************************
Measurement finished
Mean Circularitiy: 0.924064
Mean Area: 1045.06
Mean FL-1 intensity: 5944.71
Mean FL-2 intensity: 9191.36
Mean FL-3 intensity: 0
frames processed: 670268
Total Time: 336827ms
Number of Runs (new bg img) 0
Frames collected: 673477
Skipped frames: 34511
Rescued Frames: 2096266
Samples DAQ: 168369378
Samples written: 336738312
Samples per Channel: 168369156
FPS: 1999.47
Samples per second: 499868
Expected samples/s: 500000
Events: 7023
Events per second: 20.8505
jumped back (PMT): 21
#Frames read, frequency
# 0 frames :847455
# 1 frames :468362
# 2 frames :8430
# 3 frames :3925
# 4 frames :3029
# 5 frames :1608
# 6 frames :1799
# 7 frames :1885
# 8 frames :1708
# 9 frames :1104
# 10 frames :967
# 11 frames :999
# 12 frames :1423
# 13 frames :990
# 14 frames :848
# 15 frames :567
# 16 frames :403
# 17 frames :307
# 18 frames :336
# 19 frames :231
# 20 frames :164
# 21 frames :106
# 22 frames :69
# 23 frames :38
# 24 frames :31
# 25 frames :22
# 26 frames :22
# 27 frames :16
# 28 frames :13
# 29 frames :7
# 30 frames :10
# 31 frames :11
# 32 frames :6
# 33 frames :3
# 34 frames :5
# 35 frames :2
# 36 frames :3
# 37 frames :2
# 38 frames :2
# 39 frames :3
# 42 frames :2
# 46 frames :1
# 54 frames :1
# 55 frames :1
# 63 frames :1
M1_para.ini
[General]Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100
[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0
#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 27.1423
parameters.txt
#fRT-DC Configuration file# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0
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Additional Information
Field | Value |
---|---|
license | Creative Commons Public Domain Dedication |
created | 3 months ago |
etag | 20cc18e60270b345ac5777e1aa783945 |
format | RT-FDC |
id | 7fcbd2b7-43ce-4562-b37f-1981b1a2d08f |
metadata modified | 3 months ago |
mimetype | RT-DC |
package id | c94d5a65-0960-48b5-b77f-9c4c10e4e346 |
position | 0 |
s3 available | True |
s3 url | https://objectstore.hpccloud.mpcdf.mpg.de/circle-8a23b5b7-6d86-46b7-9833-62373e9ec110/resource/7fc/bd2/b7-43ce-4562-b37f-1981b1a2d08f |
sha256 | ad6e046bfb9e132d15a5326084731a15baadef06086f7cb4f76fd03a25a036b8 |
size | 123.4 MiB |
dc:experiment:date | 2017-05-18 |
dc:experiment:event count | 7,021 |
dc:experiment:run identifier | None-6519 |
dc:experiment:run index | 1 |
dc:experiment:sample | 20170518_1456_dsRNA_H5_faster |
dc:experiment:time | 14:56:27 |
dc:fluorescence:bit depth | 16 |
dc:fluorescence:channel 1 name | FL1 |
dc:fluorescence:channel 2 name | FL2 |
dc:fluorescence:channel count | 2 |
dc:fluorescence:channels installed | 3 |
dc:fluorescence:laser 1 lambda | 488 |
dc:fluorescence:laser 1 power | 10 |
dc:fluorescence:laser 2 lambda | 561 |
dc:fluorescence:laser 2 power | 35 |
dc:fluorescence:laser 3 lambda | 640 |
dc:fluorescence:laser 3 power | 0 |
dc:fluorescence:laser count | 2 |
dc:fluorescence:lasers installed | 3 |
dc:fluorescence:sample rate | 500,000 |
dc:fluorescence:samples per event | 200 |
dc:fluorescence:signal max | 1 |
dc:fluorescence:signal min | -1 |
dc:fluorescence:trace median | 5 |
dc:imaging:flash device | LED (undefined) |
dc:imaging:flash duration | 2 |
dc:imaging:frame rate | 2,000 |
dc:imaging:pixel size | 0.34 |
dc:imaging:roi position x | 504 |
dc:imaging:roi position y | 472 |
dc:imaging:roi size x | 256 |
dc:imaging:roi size y | 96 |
dc:online contour:bin area min | 50 |
dc:online contour:bin kernel | 5 |
dc:online contour:bin threshold | 6 |
dc:online contour:image blur | 0 |
dc:online contour:no absdiff | True |
dc:setup:channel width | 20 |
dc:setup:chip region | channel |
dc:setup:flow rate | 0.06 |
dc:setup:module composition | Cell_Flow_2, Fluor |
dc:setup:software version | fRT-DC V1.06170206 | dclab 0.59.1 |
sp:cells:cell type | KC167 |
sp:cells:live | True |
sp:cells:organism | Drosophila melanogaster |
sp:chip:channel width | 20 |
sp:experiment:buffer osmolality | 325 |
sp:experiment:buffer ph | 6.8 |
sp:experiment:concentration | 5 |
sp:identifiers:labels | GFP tdTomato |
sp:identifiers:tags | NLS-GFP |