Click on the log titles below to expand the log entries present in this DC resource.
dclab-export_2024-07-09_18.00.05
{"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc
{
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "07afa54c7526061619f8e268ee25b4b7",
"name": "M1_20170518_1719_dsRNA_I3_fast.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-09",
"time": "16:00:26"
}
}
dclab-tdms2rtdc-warnings
LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8258 (max 8259) in '8258'.
FG_Config.mcf
[Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;
[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;
[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini
[Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6
[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini
[EVENT LOG] fRT-DC V1.06170206
Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 27.3011
Start pulse train for BG Aquisition..
Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 2.91418e+008
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 1050 frames Cell Events: 52 mean_circ: 0.933478
Ran for 1550 frames Cell Events: 77 mean_circ: 0.93561
Ran for 2050 frames Cell Events: 96 mean_circ: 0.935023
Ran for 2550 frames Cell Events: 120 mean_circ: 0.931274
Ran for 3050 frames Cell Events: 159 mean_circ: 0.931622
Ran for 4550 frames Cell Events: 256 mean_circ: 0.930197
Ran for 7050 frames Cell Events: 393 mean_circ: 0.931637
Ran for 7550 frames Cell Events: 412 mean_circ: 0.931696
Ran for 9050 frames Cell Events: 491 mean_circ: 0.933725
Ran for 9550 frames Cell Events: 513 mean_circ: 0.93396
Ran for 10550 frames Cell Events: 559 mean_circ: 0.934589
Ran for 12050 frames Cell Events: 650 mean_circ: 0.935565
Ran for 12550 frames Cell Events: 680 mean_circ: 0.934988
Ran for 13050 frames Cell Events: 706 mean_circ: 0.934502
Ran for 14550 frames Cell Events: 771 mean_circ: 0.934352
Ran for 16050 frames Cell Events: 858 mean_circ: 0.935278
Ran for 16550 frames Cell Events: 885 mean_circ: 0.934949
Ran for 17550 frames Cell Events: 923 mean_circ: 0.934839
Ran for 18050 frames Cell Events: 942 mean_circ: 0.935164
Ran for 18550 frames Cell Events: 964 mean_circ: 0.935343
Ran for 19050 frames Cell Events: 989 mean_circ: 0.93543
Ran for 20550 frames Cell Events: 1065 mean_circ: 0.935239
Ran for 21550 frames Cell Events: 1103 mean_circ: 0.935232
Ran for 23050 frames Cell Events: 1186 mean_circ: 0.935256
Ran for 24050 frames Cell Events: 1221 mean_circ: 0.935298
Ran for 25550 frames Cell Events: 1312 mean_circ: 0.934448
Ran for 27050 frames Cell Events: 1360 mean_circ: 0.93419
Ran for 27550 frames Cell Events: 1384 mean_circ: 0.934358
Ran for 28050 frames Cell Events: 1399 mean_circ: 0.934431
Ran for 29550 frames Cell Events: 1470 mean_circ: 0.934558
Ran for 30050 frames Cell Events: 1496 mean_circ: 0.934652
Ran for 31550 frames Cell Events: 1584 mean_circ: 0.934457
Ran for 32050 frames Cell Events: 1612 mean_circ: 0.934051
Ran for 32550 frames Cell Events: 1637 mean_circ: 0.933891
Ran for 33050 frames Cell Events: 1657 mean_circ: 0.933938
Ran for 33550 frames Cell Events: 1678 mean_circ: 0.934041
Ran for 34050 frames Cell Events: 1693 mean_circ: 0.934088
Ran for 34550 frames Cell Events: 1717 mean_circ: 0.934264
Ran for 35050 frames Cell Events: 1735 mean_circ: 0.934368
Ran for 35550 frames Cell Events: 1760 mean_circ: 0.934262
Ran for 36050 frames Cell Events: 1783 mean_circ: 0.934265
Ran for 36550 frames Cell Events: 1819 mean_circ: 0.934122
Ran for 37550 frames Cell Events: 1896 mean_circ: 0.934124
Ran for 38550 frames Cell Events: 1970 mean_circ: 0.934234
Ran for 39550 frames Cell Events: 2024 mean_circ: 0.934462
Ran for 40550 frames Cell Events: 2077 mean_circ: 0.934119
Ran for 41050 frames Cell Events: 2115 mean_circ: 0.934106
Ran for 42550 frames Cell Events: 2192 mean_circ: 0.93378
Ran for 43550 frames Cell Events: 2249 mean_circ: 0.9337
Ran for 44050 frames Cell Events: 2269 mean_circ: 0.933854
Ran for 44550 frames Cell Events: 2294 mean_circ: 0.933793
Ran for 45050 frames Cell Events: 2316 mean_circ: 0.933672
Ran for 47050 frames Cell Events: 2416 mean_circ: 0.933581
Ran for 47550 frames Cell Events: 2446 mean_circ: 0.933699
Ran for 48050 frames Cell Events: 2469 mean_circ: 0.933701
Ran for 48550 frames Cell Events: 2492 mean_circ: 0.933797
Ran for 49050 frames Cell Events: 2520 mean_circ: 0.933894
Ran for 50050 frames Cell Events: 2594 mean_circ: 0.933613
Ran for 50550 frames Cell Events: 2622 mean_circ: 0.933621
Ran for 52550 frames Cell Events: 2759 mean_circ: 0.933407
Ran for 53050 frames Cell Events: 2772 mean_circ: 0.933409
Ran for 54050 frames Cell Events: 2832 mean_circ: 0.933271
Ran for 55050 frames Cell Events: 2877 mean_circ: 0.933304
Ran for 56050 frames Cell Events: 2932 mean_circ: 0.933119
Ran for 57050 frames Cell Events: 2965 mean_circ: 0.933064
Ran for 59050 frames Cell Events: 3084 mean_circ: 0.932912
Ran for 59550 frames Cell Events: 3103 mean_circ: 0.932779
Ran for 60050 frames Cell Events: 3125 mean_circ: 0.932814
Ran for 60550 frames Cell Events: 3141 mean_circ: 0.932774
Ran for 61050 frames Cell Events: 3164 mean_circ: 0.932857
Ran for 61550 frames Cell Events: 3184 mean_circ: 0.93262
Ran for 62550 frames Cell Events: 3232 mean_circ: 0.932558
Ran for 63050 frames Cell Events: 3257 mean_circ: 0.932572
Ran for 64050 frames Cell Events: 3304 mean_circ: 0.932664
Ran for 65050 frames Cell Events: 3356 mean_circ: 0.932857
Ran for 65550 frames Cell Events: 3384 mean_circ: 0.932958
Ran for 66050 frames Cell Events: 3413 mean_circ: 0.932939
Ran for 67050 frames Cell Events: 3487 mean_circ: 0.932749
Ran for 67550 frames Cell Events: 3521 mean_circ: 0.932789
Ran for 69050 frames Cell Events: 3606 mean_circ: 0.932625
Ran for 70050 frames Cell Events: 3667 mean_circ: 0.932595
Ran for 70550 frames Cell Events: 3693 mean_circ: 0.932526
Ran for 71050 frames Cell Events: 3736 mean_circ: 0.932431
Ran for 73050 frames Cell Events: 3865 mean_circ: 0.932397
Ran for 75550 frames Cell Events: 4004 mean_circ: 0.932184
Ran for 76550 frames Cell Events: 4060 mean_circ: 0.932321
Ran for 77050 frames Cell Events: 4088 mean_circ: 0.932298
Ran for 77550 frames Cell Events: 4127 mean_circ: 0.93234
Ran for 78050 frames Cell Events: 4165 mean_circ: 0.932265
Ran for 79050 frames Cell Events: 4212 mean_circ: 0.932125
Ran for 79550 frames Cell Events: 4236 mean_circ: 0.932201
Ran for 80050 frames Cell Events: 4270 mean_circ: 0.93224
Ran for 80550 frames Cell Events: 4289 mean_circ: 0.932296
Ran for 81550 frames Cell Events: 4330 mean_circ: 0.932254
Ran for 82050 frames Cell Events: 4360 mean_circ: 0.93227
Ran for 82550 frames Cell Events: 4387 mean_circ: 0.932164
Ran for 83050 frames Cell Events: 4411 mean_circ: 0.932168
Ran for 84550 frames Cell Events: 4478 mean_circ: 0.932209
Ran for 85550 frames Cell Events: 4538 mean_circ: 0.932142
Ran for 86050 frames Cell Events: 4561 mean_circ: 0.932177
Ran for 86550 frames Cell Events: 4584 mean_circ: 0.932115
Ran for 87550 frames Cell Events: 4623 mean_circ: 0.932154
Ran for 88550 frames Cell Events: 4677 mean_circ: 0.932168
Ran for 89050 frames Cell Events: 4709 mean_circ: 0.932153
Ran for 89550 frames Cell Events: 4731 mean_circ: 0.932235
Ran for 90050 frames Cell Events: 4761 mean_circ: 0.9322
Ran for 90550 frames Cell Events: 4791 mean_circ: 0.932174
Ran for 91050 frames Cell Events: 4811 mean_circ: 0.932252
Ran for 91550 frames Cell Events: 4829 mean_circ: 0.932257
Ran for 92050 frames Cell Events: 4867 mean_circ: 0.932263
Ran for 93050 frames Cell Events: 4920 mean_circ: 0.932183
Ran for 93550 frames Cell Events: 4949 mean_circ: 0.932223
Ran for 96550 frames Cell Events: 5129 mean_circ: 0.932268
Ran for 97050 frames Cell Events: 5151 mean_circ: 0.93232
Ran for 98050 frames Cell Events: 5200 mean_circ: 0.932292
Ran for 99550 frames Cell Events: 5274 mean_circ: 0.932249
Ran for 100050 frames Cell Events: 5305 mean_circ: 0.932251
Ran for 101050 frames Cell Events: 5358 mean_circ: 0.932151
Ran for 101550 frames Cell Events: 5389 mean_circ: 0.932192
Ran for 102550 frames Cell Events: 5443 mean_circ: 0.932199
Ran for 103050 frames Cell Events: 5463 mean_circ: 0.932233
Ran for 103550 frames Cell Events: 5483 mean_circ: 0.932151
Ran for 104550 frames Cell Events: 5531 mean_circ: 0.93216
Ran for 105050 frames Cell Events: 5559 mean_circ: 0.932095
Ran for 105550 frames Cell Events: 5582 mean_circ: 0.932025
Ran for 106550 frames Cell Events: 5636 mean_circ: 0.932002
Ran for 107050 frames Cell Events: 5672 mean_circ: 0.932015
Ran for 108050 frames Cell Events: 5732 mean_circ: 0.932014
Ran for 108550 frames Cell Events: 5755 mean_circ: 0.932003
Ran for 110550 frames Cell Events: 5842 mean_circ: 0.931942
Ran for 111050 frames Cell Events: 5867 mean_circ: 0.931966
Ran for 111550 frames Cell Events: 5895 mean_circ: 0.931977
Ran for 112050 frames Cell Events: 5921 mean_circ: 0.932007
Ran for 114050 frames Cell Events: 6061 mean_circ: 0.931814
Ran for 115050 frames Cell Events: 6133 mean_circ: 0.931864
Ran for 115550 frames Cell Events: 6162 mean_circ: 0.931864
Ran for 117550 frames Cell Events: 6262 mean_circ: 0.931863
Ran for 118050 frames Cell Events: 6286 mean_circ: 0.931857
Ran for 118550 frames Cell Events: 6315 mean_circ: 0.931806
Ran for 119050 frames Cell Events: 6354 mean_circ: 0.931789
Ran for 119550 frames Cell Events: 6373 mean_circ: 0.931788
Ran for 120050 frames Cell Events: 6402 mean_circ: 0.931771
Ran for 121050 frames Cell Events: 6453 mean_circ: 0.931781
Ran for 122050 frames Cell Events: 6521 mean_circ: 0.931858
Ran for 123550 frames Cell Events: 6585 mean_circ: 0.931795
Ran for 125050 frames Cell Events: 6679 mean_circ: 0.931759
Ran for 127050 frames Cell Events: 6813 mean_circ: 0.931731
Ran for 127550 frames Cell Events: 6835 mean_circ: 0.9317
Ran for 128550 frames Cell Events: 6899 mean_circ: 0.931686
Ran for 130550 frames Cell Events: 7058 mean_circ: 0.931666
Ran for 132050 frames Cell Events: 7167 mean_circ: 0.931622
Ran for 132550 frames Cell Events: 7187 mean_circ: 0.931622
Ran for 134050 frames Cell Events: 7261 mean_circ: 0.931602
Ran for 134550 frames Cell Events: 7282 mean_circ: 0.931625
Ran for 135050 frames Cell Events: 7310 mean_circ: 0.931632
Ran for 136550 frames Cell Events: 7397 mean_circ: 0.931609
Ran for 137550 frames Cell Events: 7460 mean_circ: 0.931646
Ran for 138050 frames Cell Events: 7478 mean_circ: 0.931658
Ran for 138550 frames Cell Events: 7490 mean_circ: 0.931694
Ran for 139050 frames Cell Events: 7514 mean_circ: 0.931711
Ran for 139550 frames Cell Events: 7544 mean_circ: 0.931705
Ran for 140550 frames Cell Events: 7608 mean_circ: 0.931637
Ran for 141050 frames Cell Events: 7638 mean_circ: 0.931639
Ran for 141550 frames Cell Events: 7665 mean_circ: 0.931642
Ran for 143050 frames Cell Events: 7751 mean_circ: 0.931708
Ran for 144550 frames Cell Events: 7837 mean_circ: 0.931764
Ran for 145550 frames Cell Events: 7892 mean_circ: 0.931795
Ran for 146050 frames Cell Events: 7920 mean_circ: 0.931804
Ran for 147050 frames Cell Events: 8005 mean_circ: 0.931804
Ran for 148550 frames Cell Events: 8114 mean_circ: 0.931766
Ran for 149550 frames Cell Events: 8153 mean_circ: 0.931653
Ran for 150050 frames Cell Events: 8186 mean_circ: 0.931688
End this measurment..
Stopping time: 2.91494e+008
****************************************
Measurement finished
Mean Circularitiy: 0.931669
Mean Area: 654.527
Mean FL-1 intensity: 2967.02
Mean FL-2 intensity: 5286.13
Mean FL-3 intensity: 0
frames processed: 148872
Total Time: 75547.6ms
Number of Runs (new bg img) 0
Frames collected: 150931
Skipped frames: 58196
Rescued Frames: 3135798
Samples DAQ: 37737718
Samples written: 75465472
Samples per Channel: 37732736
FPS: 1997.83
Samples per second: 499456
Expected samples/s: 500000
Events: 8259
Events per second: 109.322
jumped back (PMT): 23
#Frames read, frequency
# 0 frames :226879
# 1 frames :62277
# 2 frames :3068
# 3 frames :1018
# 4 frames :490
# 5 frames :199
# 6 frames :2087
# 7 frames :1438
# 8 frames :1011
# 9 frames :574
# 10 frames :430
# 11 frames :391
# 12 frames :271
# 13 frames :170
# 14 frames :233
# 15 frames :200
# 16 frames :158
# 17 frames :104
# 18 frames :78
# 19 frames :58
# 20 frames :59
# 21 frames :63
# 22 frames :57
# 23 frames :35
# 24 frames :41
# 25 frames :28
# 26 frames :19
# 27 frames :19
# 28 frames :23
# 29 frames :13
# 30 frames :16
# 31 frames :22
# 32 frames :5
# 33 frames :8
# 34 frames :6
# 35 frames :4
# 36 frames :2
# 37 frames :2
# 38 frames :4
# 39 frames :2
# 40 frames :5
# 41 frames :2
# 42 frames :4
# 43 frames :2
# 44 frames :2
# 45 frames :2
# 46 frames :1
# 47 frames :3
# 51 frames :1
# 53 frames :1
# 55 frames :1
# 57 frames :1
# 58 frames :1
# 63 frames :1
# 64 frames :1
M1_para.ini
[General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100
[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0
#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 27.3011
parameters.txt
#fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0