Click on the log titles below to expand the log entries present in this DC resource.
dclab-export_2024-07-09_18.23.02
{"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc
{
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "10e65f779cf9c4c2b6d9501bafc7a459",
"name": "M1_20170519_1341_dsRNA_K3_faster.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-09",
"time": "16:23:32"
}
}
dclab-tdms2rtdc-warnings
LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 9394 (max 9395) in '9394'.
FG_Config.mcf
[Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;
[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;
[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini
[Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6
[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini
[EVENT LOG] fRT-DC V1.06170206
Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 27.3337
Start pulse train for BG Aquisition..
Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 3.64748e+008
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 550 frames Cell Events: 20 mean_circ: 0.888846
Ran for 2050 frames Cell Events: 95 mean_circ: 0.905424
Ran for 2550 frames Cell Events: 109 mean_circ: 0.903203
Ran for 3050 frames Cell Events: 134 mean_circ: 0.904788
Ran for 4050 frames Cell Events: 150 mean_circ: 0.904854
Ran for 4550 frames Cell Events: 161 mean_circ: 0.903547
Ran for 7550 frames Cell Events: 285 mean_circ: 0.898103
Ran for 8050 frames Cell Events: 302 mean_circ: 0.89839
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Ran for 10550 frames Cell Events: 397 mean_circ: 0.899549
Ran for 11550 frames Cell Events: 439 mean_circ: 0.899328
Ran for 12050 frames Cell Events: 447 mean_circ: 0.899507
Ran for 12550 frames Cell Events: 466 mean_circ: 0.899042
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Ran for 13550 frames Cell Events: 500 mean_circ: 0.898713
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Ran for 19050 frames Cell Events: 719 mean_circ: 0.89485
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Ran for 73050 frames Cell Events: 2843 mean_circ: 0.895002
Ran for 73550 frames Cell Events: 2857 mean_circ: 0.895055
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Ran for 76550 frames Cell Events: 2968 mean_circ: 0.895367
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Ran for 77550 frames Cell Events: 2995 mean_circ: 0.895419
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Ran for 83050 frames Cell Events: 3207 mean_circ: 0.895456
Ran for 83550 frames Cell Events: 3221 mean_circ: 0.895509
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Ran for 86550 frames Cell Events: 3354 mean_circ: 0.895172
Ran for 87050 frames Cell Events: 3375 mean_circ: 0.895394
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Ran for 90550 frames Cell Events: 3476 mean_circ: 0.895487
Ran for 91050 frames Cell Events: 3487 mean_circ: 0.895532
Ran for 91550 frames Cell Events: 3495 mean_circ: 0.895553
Ran for 93550 frames Cell Events: 3588 mean_circ: 0.895725
Ran for 94050 frames Cell Events: 3607 mean_circ: 0.895646
Ran for 94550 frames Cell Events: 3619 mean_circ: 0.895624
Ran for 95050 frames Cell Events: 3647 mean_circ: 0.895626
Ran for 97550 frames Cell Events: 3781 mean_circ: 0.895631
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Ran for 99050 frames Cell Events: 3831 mean_circ: 0.895443
Ran for 99550 frames Cell Events: 3854 mean_circ: 0.895437
Ran for 100050 frames Cell Events: 3870 mean_circ: 0.895336
Ran for 100550 frames Cell Events: 3894 mean_circ: 0.895116
Ran for 101050 frames Cell Events: 3910 mean_circ: 0.895066
Ran for 101550 frames Cell Events: 3940 mean_circ: 0.895082
Ran for 102050 frames Cell Events: 3958 mean_circ: 0.895077
Ran for 102550 frames Cell Events: 3971 mean_circ: 0.895072
Ran for 103050 frames Cell Events: 3987 mean_circ: 0.895098
Ran for 103550 frames Cell Events: 4009 mean_circ: 0.895023
Ran for 104550 frames Cell Events: 4050 mean_circ: 0.894997
Ran for 105050 frames Cell Events: 4074 mean_circ: 0.894973
Ran for 105550 frames Cell Events: 4091 mean_circ: 0.895013
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Ran for 106550 frames Cell Events: 4138 mean_circ: 0.895199
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Ran for 120050 frames Cell Events: 4675 mean_circ: 0.895293
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Ran for 121550 frames Cell Events: 4740 mean_circ: 0.895262
Ran for 122050 frames Cell Events: 4759 mean_circ: 0.895162
Ran for 122550 frames Cell Events: 4776 mean_circ: 0.89516
Ran for 123050 frames Cell Events: 4800 mean_circ: 0.89515
Ran for 124550 frames Cell Events: 4859 mean_circ: 0.895166
Ran for 125550 frames Cell Events: 4906 mean_circ: 0.895147
Ran for 127050 frames Cell Events: 4962 mean_circ: 0.895001
Ran for 128050 frames Cell Events: 5003 mean_circ: 0.894917
Ran for 129050 frames Cell Events: 5047 mean_circ: 0.894979
Ran for 129550 frames Cell Events: 5054 mean_circ: 0.895029
Ran for 131050 frames Cell Events: 5108 mean_circ: 0.895058
Ran for 131550 frames Cell Events: 5138 mean_circ: 0.895004
Ran for 132050 frames Cell Events: 5159 mean_circ: 0.895138
Ran for 132550 frames Cell Events: 5191 mean_circ: 0.895013
Ran for 133050 frames Cell Events: 5207 mean_circ: 0.894872
Ran for 133550 frames Cell Events: 5225 mean_circ: 0.894744
Ran for 134050 frames Cell Events: 5241 mean_circ: 0.894736
Ran for 136050 frames Cell Events: 5347 mean_circ: 0.894888
Ran for 136550 frames Cell Events: 5371 mean_circ: 0.894868
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Ran for 141050 frames Cell Events: 5595 mean_circ: 0.89492
Ran for 142050 frames Cell Events: 5632 mean_circ: 0.894838
Ran for 143550 frames Cell Events: 5660 mean_circ: 0.894881
Ran for 144050 frames Cell Events: 5687 mean_circ: 0.894827
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Ran for 146550 frames Cell Events: 5796 mean_circ: 0.894969
Ran for 147050 frames Cell Events: 5818 mean_circ: 0.894978
Ran for 147550 frames Cell Events: 5839 mean_circ: 0.894997
Ran for 148550 frames Cell Events: 5879 mean_circ: 0.894974
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Ran for 152050 frames Cell Events: 6035 mean_circ: 0.894767
Ran for 152550 frames Cell Events: 6053 mean_circ: 0.894818
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Ran for 183550 frames Cell Events: 7211 mean_circ: 0.894647
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Ran for 196550 frames Cell Events: 7695 mean_circ: 0.894349
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Ran for 197550 frames Cell Events: 7732 mean_circ: 0.894241
Ran for 198050 frames Cell Events: 7752 mean_circ: 0.894191
Ran for 198550 frames Cell Events: 7781 mean_circ: 0.894165
Ran for 199050 frames Cell Events: 7810 mean_circ: 0.894097
Ran for 199550 frames Cell Events: 7832 mean_circ: 0.894024
Ran for 200550 frames Cell Events: 7880 mean_circ: 0.894045
Ran for 201050 frames Cell Events: 7903 mean_circ: 0.894137
Ran for 201550 frames Cell Events: 7932 mean_circ: 0.894298
Ran for 202050 frames Cell Events: 7954 mean_circ: 0.894301
Ran for 202550 frames Cell Events: 7973 mean_circ: 0.894301
Ran for 203050 frames Cell Events: 7994 mean_circ: 0.894238
Ran for 203550 frames Cell Events: 8013 mean_circ: 0.894195
Ran for 204550 frames Cell Events: 8062 mean_circ: 0.894262
Ran for 205050 frames Cell Events: 8093 mean_circ: 0.894312
Ran for 206550 frames Cell Events: 8151 mean_circ: 0.894211
Ran for 207050 frames Cell Events: 8186 mean_circ: 0.894192
Ran for 207550 frames Cell Events: 8202 mean_circ: 0.894241
Ran for 209050 frames Cell Events: 8281 mean_circ: 0.894212
Ran for 209550 frames Cell Events: 8313 mean_circ: 0.894217
Ran for 210050 frames Cell Events: 8333 mean_circ: 0.894242
Ran for 211550 frames Cell Events: 8391 mean_circ: 0.894228
Ran for 212050 frames Cell Events: 8403 mean_circ: 0.894258
Ran for 212550 frames Cell Events: 8426 mean_circ: 0.894192
Ran for 213050 frames Cell Events: 8442 mean_circ: 0.894147
Ran for 213550 frames Cell Events: 8460 mean_circ: 0.894115
Ran for 215050 frames Cell Events: 8537 mean_circ: 0.893941
Ran for 215550 frames Cell Events: 8553 mean_circ: 0.893871
Ran for 216550 frames Cell Events: 8596 mean_circ: 0.893896
Ran for 217050 frames Cell Events: 8621 mean_circ: 0.893859
Ran for 217550 frames Cell Events: 8636 mean_circ: 0.893877
Ran for 218550 frames Cell Events: 8670 mean_circ: 0.89385
Ran for 219550 frames Cell Events: 8712 mean_circ: 0.893855
Ran for 220050 frames Cell Events: 8738 mean_circ: 0.893912
Ran for 220550 frames Cell Events: 8763 mean_circ: 0.893849
Ran for 221550 frames Cell Events: 8796 mean_circ: 0.893834
Ran for 222050 frames Cell Events: 8826 mean_circ: 0.893853
Ran for 223550 frames Cell Events: 8882 mean_circ: 0.893819
Ran for 224050 frames Cell Events: 8889 mean_circ: 0.893797
Ran for 225050 frames Cell Events: 8950 mean_circ: 0.893771
Ran for 225550 frames Cell Events: 8972 mean_circ: 0.893755
Ran for 226550 frames Cell Events: 8995 mean_circ: 0.893776
Ran for 227550 frames Cell Events: 9047 mean_circ: 0.893766
Ran for 228550 frames Cell Events: 9084 mean_circ: 0.893763
Ran for 229550 frames Cell Events: 9127 mean_circ: 0.893802
Ran for 230050 frames Cell Events: 9144 mean_circ: 0.893775
Ran for 231050 frames Cell Events: 9156 mean_circ: 0.893768
Ran for 232050 frames Cell Events: 9194 mean_circ: 0.893747
Ran for 232550 frames Cell Events: 9217 mean_circ: 0.893792
Ran for 233050 frames Cell Events: 9231 mean_circ: 0.893742
Ran for 234550 frames Cell Events: 9277 mean_circ: 0.893708
Ran for 235050 frames Cell Events: 9298 mean_circ: 0.893676
Ran for 235550 frames Cell Events: 9309 mean_circ: 0.893685
Ran for 236050 frames Cell Events: 9322 mean_circ: 0.89367
Ran for 236550 frames Cell Events: 9344 mean_circ: 0.893666
End this measurment..
Stopping time: 3.64867e+008
****************************************
Measurement finished
Mean Circularitiy: 0.893689
Mean Area: 603.351
Mean FL-1 intensity: 2810.27
Mean FL-2 intensity: 4485.46
Mean FL-3 intensity: 0
frames processed: 235556
Total Time: 119029ms
Number of Runs (new bg img) 0
Frames collected: 237889
Skipped frames: 11205
Rescued Frames: 693379
Samples DAQ: 59472138
Samples written: 118944170
Samples per Channel: 59472085
FPS: 1998.58
Samples per second: 499643
Expected samples/s: 500000
Events: 9395
Events per second: 78.9303
jumped back (PMT): 22
#Frames read, frequency
# 0 frames :326304
# 1 frames :131160
# 2 frames :5660
# 3 frames :1501
# 4 frames :674
# 5 frames :245
# 6 frames :3934
# 7 frames :2047
# 8 frames :1443
# 9 frames :700
# 10 frames :391
# 11 frames :262
# 12 frames :350
# 13 frames :168
# 14 frames :237
# 15 frames :173
# 16 frames :121
# 17 frames :85
# 18 frames :61
# 19 frames :30
# 20 frames :36
# 21 frames :35
# 22 frames :32
# 23 frames :15
# 24 frames :12
# 25 frames :12
# 26 frames :12
# 27 frames :10
# 28 frames :1
# 29 frames :2
# 30 frames :3
# 31 frames :5
# 32 frames :6
# 33 frames :2
# 34 frames :1
# 35 frames :3
# 37 frames :1
# 38 frames :1
# 39 frames :1
# 41 frames :1
# 42 frames :2
# 46 frames :1
# 50 frames :1
# 54 frames :1
# 68 frames :1
# 91 frames :1
M1_para.ini
[General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100
[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0
#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 27.3337
parameters.txt
#fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0