Click on the log titles below to expand the log entries present in this DC resource.
dclab-export_2024-07-09_18.44.05
{"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc
{
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "1be3b35c61437cd4012cd799bc3feb12",
"name": "M1_20170519_1714_dsRNA_M3_faster.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-09",
"time": "16:44:35"
}
}
dclab-tdms2rtdc-warnings
LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8621 (max 8622) in '8621'.
FG_Config.mcf
[Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;
[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;
[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini
[Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6
[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini
[EVENT LOG] fRT-DC V1.06170206
Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 26.9199
Start pulse train for BG Aquisition..
Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 3.775e+008
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 1050 frames Cell Events: 39 mean_circ: 0.937062
Ran for 2050 frames Cell Events: 78 mean_circ: 0.935151
Ran for 2550 frames Cell Events: 97 mean_circ: 0.933109
Ran for 3550 frames Cell Events: 130 mean_circ: 0.931198
Ran for 5050 frames Cell Events: 175 mean_circ: 0.930397
Ran for 6050 frames Cell Events: 216 mean_circ: 0.930971
Ran for 8050 frames Cell Events: 282 mean_circ: 0.931111
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Ran for 9050 frames Cell Events: 326 mean_circ: 0.93037
Ran for 9550 frames Cell Events: 355 mean_circ: 0.930012
Ran for 10050 frames Cell Events: 375 mean_circ: 0.929503
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Ran for 11550 frames Cell Events: 437 mean_circ: 0.93066
Ran for 12050 frames Cell Events: 448 mean_circ: 0.93115
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Ran for 92050 frames Cell Events: 3607 mean_circ: 0.929262
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Ran for 93050 frames Cell Events: 3652 mean_circ: 0.929324
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Ran for 94050 frames Cell Events: 3686 mean_circ: 0.929412
Ran for 95550 frames Cell Events: 3757 mean_circ: 0.929322
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Ran for 96550 frames Cell Events: 3810 mean_circ: 0.929161
Ran for 97050 frames Cell Events: 3847 mean_circ: 0.929224
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Ran for 98050 frames Cell Events: 3900 mean_circ: 0.929257
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Ran for 102050 frames Cell Events: 4064 mean_circ: 0.929154
Ran for 102550 frames Cell Events: 4088 mean_circ: 0.929138
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Ran for 122550 frames Cell Events: 4956 mean_circ: 0.929737
Ran for 123050 frames Cell Events: 4980 mean_circ: 0.929779
Ran for 124050 frames Cell Events: 5020 mean_circ: 0.929737
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Ran for 126050 frames Cell Events: 5104 mean_circ: 0.9298
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Ran for 128050 frames Cell Events: 5186 mean_circ: 0.929887
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Ran for 132550 frames Cell Events: 5376 mean_circ: 0.929607
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Ran for 142050 frames Cell Events: 5798 mean_circ: 0.929567
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Ran for 194550 frames Cell Events: 8140 mean_circ: 0.929869
Ran for 196050 frames Cell Events: 8205 mean_circ: 0.929858
Ran for 197050 frames Cell Events: 8247 mean_circ: 0.929901
Ran for 197550 frames Cell Events: 8263 mean_circ: 0.929881
Ran for 198050 frames Cell Events: 8279 mean_circ: 0.929868
Ran for 199050 frames Cell Events: 8335 mean_circ: 0.929831
Ran for 199550 frames Cell Events: 8348 mean_circ: 0.929827
Ran for 200550 frames Cell Events: 8378 mean_circ: 0.929848
Ran for 202050 frames Cell Events: 8442 mean_circ: 0.929788
Ran for 202550 frames Cell Events: 8452 mean_circ: 0.92978
Ran for 203050 frames Cell Events: 8474 mean_circ: 0.929805
Ran for 203550 frames Cell Events: 8499 mean_circ: 0.929839
Ran for 206050 frames Cell Events: 8562 mean_circ: 0.929845
Ran for 206550 frames Cell Events: 8581 mean_circ: 0.929811
End this measurment..
Stopping time: 3.77604e+008
****************************************
Measurement finished
Mean Circularitiy: 0.929793
Mean Area: 627.567
Mean FL-1 intensity: 2865.62
Mean FL-2 intensity: 4740.22
Mean FL-3 intensity: 0
frames processed: 206341
Total Time: 103842ms
Number of Runs (new bg img) 0
Frames collected: 207517
Skipped frames: 73052
Rescued Frames: 3681939
Samples DAQ: 51879381
Samples written: 103758532
Samples per Channel: 51879266
FPS: 1998.39
Samples per second: 499598
Expected samples/s: 500000
Events: 8622
Events per second: 83.03
jumped back (PMT): 18
#Frames read, frequency
# 0 frames :292390
# 1 frames :106760
# 2 frames :4628
# 3 frames :1274
# 4 frames :689
# 5 frames :242
# 6 frames :2394
# 7 frames :2057
# 8 frames :1272
# 9 frames :664
# 10 frames :658
# 11 frames :313
# 12 frames :329
# 13 frames :222
# 14 frames :210
# 15 frames :172
# 16 frames :158
# 17 frames :92
# 18 frames :81
# 19 frames :52
# 20 frames :50
# 21 frames :52
# 22 frames :30
# 23 frames :33
# 24 frames :22
# 25 frames :23
# 26 frames :22
# 27 frames :15
# 28 frames :15
# 29 frames :13
# 30 frames :18
# 31 frames :7
# 32 frames :3
# 33 frames :5
# 34 frames :1
# 35 frames :3
# 36 frames :4
# 37 frames :3
# 38 frames :1
# 39 frames :3
# 40 frames :1
# 41 frames :5
# 42 frames :1
# 43 frames :1
# 45 frames :1
# 46 frames :3
# 47 frames :3
# 48 frames :1
# 50 frames :1
# 51 frames :1
# 67 frames :1
M1_para.ini
[General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100
[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0
#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 26.9199
parameters.txt
#fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0