Click on the log titles below to expand the log entries present in this DC resource.
dclab-export_2024-07-09_18.50.15
{"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc
{
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "a2bf77637d76e27fdc720bd5ab42dfe4",
"name": "M1_20170521_1518_dsRNA_A8_faster.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-09",
"time": "16:50:43"
}
}
dclab-tdms2rtdc-warnings
LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8030 (max 8031) in '8030'.
FG_Config.mcf
[Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;
[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;
[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini
[Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6
[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini
[EVENT LOG] fRT-DC V1.06170206
Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 24.5395
Start pulse train for BG Aquisition..
Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 5.43366e+008
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 550 frames Cell Events: 11 mean_circ: 0.918324
Ran for 1050 frames Cell Events: 19 mean_circ: 0.911852
Ran for 2050 frames Cell Events: 38 mean_circ: 0.919612
Ran for 2550 frames Cell Events: 51 mean_circ: 0.912771
Ran for 3550 frames Cell Events: 86 mean_circ: 0.914762
Ran for 4050 frames Cell Events: 107 mean_circ: 0.91799
Ran for 4550 frames Cell Events: 126 mean_circ: 0.915592
Ran for 6050 frames Cell Events: 181 mean_circ: 0.92101
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Ran for 10050 frames Cell Events: 286 mean_circ: 0.921783
Ran for 10550 frames Cell Events: 309 mean_circ: 0.921856
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Ran for 12050 frames Cell Events: 345 mean_circ: 0.922117
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Ran for 13050 frames Cell Events: 378 mean_circ: 0.922555
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Ran for 33050 frames Cell Events: 1000 mean_circ: 0.929028
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Ran for 66050 frames Cell Events: 2030 mean_circ: 0.92905
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Ran for 67050 frames Cell Events: 2063 mean_circ: 0.928911
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Ran for 77550 frames Cell Events: 2392 mean_circ: 0.928766
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Ran for 83550 frames Cell Events: 2609 mean_circ: 0.928692
Ran for 84050 frames Cell Events: 2636 mean_circ: 0.928523
Ran for 84550 frames Cell Events: 2657 mean_circ: 0.928477
Ran for 85050 frames Cell Events: 2675 mean_circ: 0.92858
Ran for 86550 frames Cell Events: 2724 mean_circ: 0.928558
Ran for 88050 frames Cell Events: 2773 mean_circ: 0.928424
Ran for 89550 frames Cell Events: 2807 mean_circ: 0.928202
Ran for 90050 frames Cell Events: 2827 mean_circ: 0.9283
Ran for 90550 frames Cell Events: 2850 mean_circ: 0.928206
Ran for 91050 frames Cell Events: 2866 mean_circ: 0.928222
Ran for 92050 frames Cell Events: 2900 mean_circ: 0.928376
Ran for 93050 frames Cell Events: 2927 mean_circ: 0.928376
Ran for 93550 frames Cell Events: 2942 mean_circ: 0.928321
Ran for 94550 frames Cell Events: 2972 mean_circ: 0.92845
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Ran for 95550 frames Cell Events: 3004 mean_circ: 0.92835
Ran for 96050 frames Cell Events: 3022 mean_circ: 0.928308
Ran for 96550 frames Cell Events: 3038 mean_circ: 0.928302
Ran for 97050 frames Cell Events: 3052 mean_circ: 0.928272
Ran for 97550 frames Cell Events: 3062 mean_circ: 0.928218
Ran for 98050 frames Cell Events: 3077 mean_circ: 0.928139
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Ran for 99550 frames Cell Events: 3134 mean_circ: 0.928088
Ran for 100050 frames Cell Events: 3145 mean_circ: 0.928113
Ran for 100550 frames Cell Events: 3161 mean_circ: 0.928194
Ran for 102050 frames Cell Events: 3207 mean_circ: 0.928135
Ran for 103050 frames Cell Events: 3236 mean_circ: 0.927992
Ran for 104050 frames Cell Events: 3261 mean_circ: 0.92802
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Ran for 106550 frames Cell Events: 3337 mean_circ: 0.92782
Ran for 107050 frames Cell Events: 3349 mean_circ: 0.927755
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Ran for 117550 frames Cell Events: 3677 mean_circ: 0.927705
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Ran for 121050 frames Cell Events: 3768 mean_circ: 0.927834
Ran for 122050 frames Cell Events: 3787 mean_circ: 0.927932
Ran for 122550 frames Cell Events: 3807 mean_circ: 0.927996
Ran for 123050 frames Cell Events: 3824 mean_circ: 0.928037
Ran for 124050 frames Cell Events: 3860 mean_circ: 0.928059
Ran for 124550 frames Cell Events: 3877 mean_circ: 0.928022
Ran for 125550 frames Cell Events: 3911 mean_circ: 0.928049
Ran for 126050 frames Cell Events: 3917 mean_circ: 0.928068
Ran for 126550 frames Cell Events: 3929 mean_circ: 0.928085
Ran for 128050 frames Cell Events: 3973 mean_circ: 0.928079
Ran for 128550 frames Cell Events: 3989 mean_circ: 0.928144
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Ran for 130550 frames Cell Events: 4055 mean_circ: 0.928179
Ran for 131050 frames Cell Events: 4069 mean_circ: 0.928233
Ran for 131550 frames Cell Events: 4085 mean_circ: 0.928246
Ran for 132050 frames Cell Events: 4100 mean_circ: 0.928225
Ran for 132550 frames Cell Events: 4109 mean_circ: 0.928258
Ran for 134050 frames Cell Events: 4163 mean_circ: 0.928297
Ran for 134550 frames Cell Events: 4174 mean_circ: 0.928283
Ran for 136050 frames Cell Events: 4221 mean_circ: 0.928264
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Ran for 141550 frames Cell Events: 4391 mean_circ: 0.928299
Ran for 142050 frames Cell Events: 4406 mean_circ: 0.928346
Ran for 143050 frames Cell Events: 4444 mean_circ: 0.928379
Ran for 144550 frames Cell Events: 4485 mean_circ: 0.928428
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Ran for 147550 frames Cell Events: 4577 mean_circ: 0.928463
Ran for 148050 frames Cell Events: 4604 mean_circ: 0.928418
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Ran for 190550 frames Cell Events: 6205 mean_circ: 0.928772
Ran for 191550 frames Cell Events: 6245 mean_circ: 0.928642
Ran for 193050 frames Cell Events: 6309 mean_circ: 0.928586
Ran for 193550 frames Cell Events: 6321 mean_circ: 0.928583
Ran for 194050 frames Cell Events: 6331 mean_circ: 0.928563
Ran for 194550 frames Cell Events: 6342 mean_circ: 0.928559
Ran for 195550 frames Cell Events: 6376 mean_circ: 0.928493
Ran for 196050 frames Cell Events: 6402 mean_circ: 0.92844
Ran for 197550 frames Cell Events: 6453 mean_circ: 0.928472
Ran for 199550 frames Cell Events: 6521 mean_circ: 0.928471
Ran for 201050 frames Cell Events: 6592 mean_circ: 0.928453
Ran for 201550 frames Cell Events: 6619 mean_circ: 0.928424
Ran for 203050 frames Cell Events: 6704 mean_circ: 0.92836
Ran for 203550 frames Cell Events: 6723 mean_circ: 0.92836
Ran for 205050 frames Cell Events: 6795 mean_circ: 0.928437
Ran for 205550 frames Cell Events: 6814 mean_circ: 0.928443
Ran for 207550 frames Cell Events: 6878 mean_circ: 0.928442
Ran for 208050 frames Cell Events: 6903 mean_circ: 0.928426
Ran for 208550 frames Cell Events: 6930 mean_circ: 0.928428
Ran for 209050 frames Cell Events: 6946 mean_circ: 0.928492
Ran for 209550 frames Cell Events: 6963 mean_circ: 0.928459
Ran for 210050 frames Cell Events: 6971 mean_circ: 0.928485
Ran for 210550 frames Cell Events: 6985 mean_circ: 0.928498
Ran for 211050 frames Cell Events: 7012 mean_circ: 0.928464
Ran for 213050 frames Cell Events: 7088 mean_circ: 0.928495
Ran for 214050 frames Cell Events: 7130 mean_circ: 0.928513
Ran for 214550 frames Cell Events: 7151 mean_circ: 0.928509
Ran for 215050 frames Cell Events: 7173 mean_circ: 0.928543
Ran for 215550 frames Cell Events: 7192 mean_circ: 0.928519
Ran for 216550 frames Cell Events: 7238 mean_circ: 0.928541
Ran for 217050 frames Cell Events: 7254 mean_circ: 0.928499
Ran for 217550 frames Cell Events: 7275 mean_circ: 0.928483
Ran for 218050 frames Cell Events: 7285 mean_circ: 0.928472
Ran for 218550 frames Cell Events: 7309 mean_circ: 0.928502
Ran for 219550 frames Cell Events: 7337 mean_circ: 0.928578
Ran for 220550 frames Cell Events: 7372 mean_circ: 0.928617
Ran for 221050 frames Cell Events: 7392 mean_circ: 0.928582
Ran for 221550 frames Cell Events: 7402 mean_circ: 0.928605
Ran for 222050 frames Cell Events: 7418 mean_circ: 0.928577
Ran for 222550 frames Cell Events: 7439 mean_circ: 0.928614
Ran for 223050 frames Cell Events: 7463 mean_circ: 0.928651
Ran for 225550 frames Cell Events: 7564 mean_circ: 0.92872
Ran for 226550 frames Cell Events: 7599 mean_circ: 0.92874
Ran for 227050 frames Cell Events: 7621 mean_circ: 0.928766
Ran for 227550 frames Cell Events: 7648 mean_circ: 0.928769
Ran for 228550 frames Cell Events: 7678 mean_circ: 0.928783
Ran for 229050 frames Cell Events: 7704 mean_circ: 0.928769
Ran for 230550 frames Cell Events: 7760 mean_circ: 0.928763
Ran for 231050 frames Cell Events: 7776 mean_circ: 0.928769
Ran for 231550 frames Cell Events: 7791 mean_circ: 0.928736
Ran for 233550 frames Cell Events: 7841 mean_circ: 0.928744
Ran for 234050 frames Cell Events: 7855 mean_circ: 0.928754
Ran for 235050 frames Cell Events: 7879 mean_circ: 0.928714
Ran for 235550 frames Cell Events: 7901 mean_circ: 0.928731
Ran for 237050 frames Cell Events: 7970 mean_circ: 0.928768
Ran for 238050 frames Cell Events: 8003 mean_circ: 0.928734
Ran for 238550 frames Cell Events: 8022 mean_circ: 0.928732
End this measurment..
Stopping time: 5.43486e+008
****************************************
Measurement finished
Mean Circularitiy: 0.928747
Mean Area: 576.027
Mean FL-1 intensity: 2913.85
Mean FL-2 intensity: 4201.38
Mean FL-3 intensity: 0
frames processed: 234837
Total Time: 119469ms
Number of Runs (new bg img) 0
Frames collected: 238818
Skipped frames: 14469
Rescued Frames: 611062
Samples DAQ: 59704463
Samples written: 119408750
Samples per Channel: 59704375
FPS: 1999
Samples per second: 499749
Expected samples/s: 500000
Events: 8031
Events per second: 67.2226
jumped back (PMT): 18
#Frames read, frequency
# 0 frames :332286
# 1 frames :128540
# 2 frames :4585
# 3 frames :1556
# 4 frames :953
# 5 frames :372
# 6 frames :2268
# 7 frames :1880
# 8 frames :1477
# 9 frames :812
# 10 frames :584
# 11 frames :407
# 12 frames :344
# 13 frames :253
# 14 frames :245
# 15 frames :199
# 16 frames :167
# 17 frames :143
# 18 frames :95
# 19 frames :60
# 20 frames :69
# 21 frames :50
# 22 frames :36
# 23 frames :33
# 24 frames :23
# 25 frames :24
# 26 frames :22
# 27 frames :16
# 28 frames :13
# 29 frames :13
# 30 frames :8
# 31 frames :6
# 32 frames :9
# 33 frames :2
# 34 frames :5
# 35 frames :3
# 36 frames :4
# 37 frames :4
# 38 frames :1
# 39 frames :1
# 40 frames :2
# 43 frames :2
# 44 frames :1
# 45 frames :2
# 46 frames :1
# 48 frames :2
# 49 frames :1
# 54 frames :1
# 55 frames :2
# 57 frames :1
# 68 frames :1
M1_para.ini
[General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100
[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0
#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 24.5395
parameters.txt
#fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0