Click on the log titles below to expand the log entries present in this DC resource.
dclab-export_2024-07-09_19.00.39
{"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc
{
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "8fb52337cb3a8203a9240a3aae19c646",
"name": "M1_20170521_1703_dsRNA_A6_col.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-09",
"time": "17:01:09"
}
}
dclab-tdms2rtdc-warnings
LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8312 (max 8313) in '8312'.
FG_Config.mcf
[Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;
[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;
[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini
[Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6
[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini
[EVENT LOG] fRT-DC V1.06170206
Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 25.0298
Start pulse train for BG Aquisition..
Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 5.49646e+008
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 550 frames Cell Events: 18 mean_circ: 0.916627
Ran for 2050 frames Cell Events: 52 mean_circ: 0.920362
Ran for 3050 frames Cell Events: 82 mean_circ: 0.911935
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Ran for 64550 frames Cell Events: 1948 mean_circ: 0.909171
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Ran for 72550 frames Cell Events: 2237 mean_circ: 0.909059
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Ran for 91550 frames Cell Events: 2805 mean_circ: 0.908243
Ran for 92050 frames Cell Events: 2819 mean_circ: 0.908172
Ran for 92550 frames Cell Events: 2830 mean_circ: 0.90815
Ran for 94050 frames Cell Events: 2883 mean_circ: 0.907973
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Ran for 100050 frames Cell Events: 3066 mean_circ: 0.90738
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Ran for 102050 frames Cell Events: 3138 mean_circ: 0.907225
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Ran for 120050 frames Cell Events: 3676 mean_circ: 0.907331
Ran for 121050 frames Cell Events: 3720 mean_circ: 0.907072
Ran for 121550 frames Cell Events: 3741 mean_circ: 0.907087
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Ran for 123550 frames Cell Events: 3816 mean_circ: 0.90722
Ran for 124050 frames Cell Events: 3833 mean_circ: 0.907195
Ran for 124550 frames Cell Events: 3847 mean_circ: 0.907209
Ran for 125050 frames Cell Events: 3862 mean_circ: 0.907197
Ran for 125550 frames Cell Events: 3874 mean_circ: 0.90714
Ran for 126050 frames Cell Events: 3888 mean_circ: 0.907123
Ran for 126550 frames Cell Events: 3906 mean_circ: 0.907117
Ran for 127550 frames Cell Events: 3932 mean_circ: 0.907105
Ran for 128050 frames Cell Events: 3946 mean_circ: 0.907165
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Ran for 131050 frames Cell Events: 4043 mean_circ: 0.907461
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Ran for 133050 frames Cell Events: 4110 mean_circ: 0.907137
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Ran for 181550 frames Cell Events: 5519 mean_circ: 0.906214
Ran for 182050 frames Cell Events: 5529 mean_circ: 0.906233
Ran for 182550 frames Cell Events: 5540 mean_circ: 0.906189
Ran for 183050 frames Cell Events: 5558 mean_circ: 0.906
Ran for 183550 frames Cell Events: 5565 mean_circ: 0.906011
Ran for 184050 frames Cell Events: 5581 mean_circ: 0.905916
Ran for 184550 frames Cell Events: 5599 mean_circ: 0.905915
Ran for 185550 frames Cell Events: 5645 mean_circ: 0.905917
Ran for 186050 frames Cell Events: 5660 mean_circ: 0.905974
Ran for 186550 frames Cell Events: 5670 mean_circ: 0.906007
Ran for 187050 frames Cell Events: 5683 mean_circ: 0.905852
Ran for 187550 frames Cell Events: 5690 mean_circ: 0.905846
Ran for 188050 frames Cell Events: 5706 mean_circ: 0.905862
Ran for 188550 frames Cell Events: 5725 mean_circ: 0.90585
Ran for 189050 frames Cell Events: 5734 mean_circ: 0.905835
Ran for 190050 frames Cell Events: 5754 mean_circ: 0.905899
Ran for 190550 frames Cell Events: 5775 mean_circ: 0.905916
Ran for 191050 frames Cell Events: 5788 mean_circ: 0.905847
Ran for 191550 frames Cell Events: 5809 mean_circ: 0.905843
Ran for 192050 frames Cell Events: 5828 mean_circ: 0.905657
Ran for 192550 frames Cell Events: 5842 mean_circ: 0.9056
Ran for 193050 frames Cell Events: 5865 mean_circ: 0.905683
Ran for 194050 frames Cell Events: 5895 mean_circ: 0.905781
Ran for 194550 frames Cell Events: 5908 mean_circ: 0.905771
Ran for 195050 frames Cell Events: 5929 mean_circ: 0.905784
Ran for 196550 frames Cell Events: 5982 mean_circ: 0.905828
Ran for 197050 frames Cell Events: 5999 mean_circ: 0.905861
Ran for 198550 frames Cell Events: 6052 mean_circ: 0.906016
Ran for 199550 frames Cell Events: 6073 mean_circ: 0.906064
Ran for 200050 frames Cell Events: 6086 mean_circ: 0.906067
Ran for 201050 frames Cell Events: 6112 mean_circ: 0.906049
Ran for 202050 frames Cell Events: 6145 mean_circ: 0.906006
Ran for 202550 frames Cell Events: 6155 mean_circ: 0.905988
Ran for 203050 frames Cell Events: 6171 mean_circ: 0.905956
Ran for 205050 frames Cell Events: 6235 mean_circ: 0.905727
Ran for 205550 frames Cell Events: 6246 mean_circ: 0.905781
Ran for 206050 frames Cell Events: 6260 mean_circ: 0.905758
Ran for 206550 frames Cell Events: 6277 mean_circ: 0.90576
Ran for 208050 frames Cell Events: 6346 mean_circ: 0.905848
Ran for 208550 frames Cell Events: 6358 mean_circ: 0.905827
Ran for 209550 frames Cell Events: 6395 mean_circ: 0.905812
Ran for 210050 frames Cell Events: 6405 mean_circ: 0.905816
Ran for 210550 frames Cell Events: 6420 mean_circ: 0.905775
Ran for 211050 frames Cell Events: 6437 mean_circ: 0.90572
Ran for 212050 frames Cell Events: 6466 mean_circ: 0.905722
Ran for 213050 frames Cell Events: 6490 mean_circ: 0.905707
Ran for 213550 frames Cell Events: 6502 mean_circ: 0.905747
Ran for 214050 frames Cell Events: 6512 mean_circ: 0.90579
Ran for 214550 frames Cell Events: 6526 mean_circ: 0.90581
Ran for 215550 frames Cell Events: 6558 mean_circ: 0.905759
Ran for 216050 frames Cell Events: 6575 mean_circ: 0.905818
Ran for 216550 frames Cell Events: 6585 mean_circ: 0.905817
Ran for 217050 frames Cell Events: 6601 mean_circ: 0.905869
Ran for 218050 frames Cell Events: 6630 mean_circ: 0.905904
Ran for 218550 frames Cell Events: 6637 mean_circ: 0.905948
Ran for 219050 frames Cell Events: 6652 mean_circ: 0.905932
Ran for 219550 frames Cell Events: 6664 mean_circ: 0.905953
Ran for 222050 frames Cell Events: 6735 mean_circ: 0.905904
Ran for 223550 frames Cell Events: 6786 mean_circ: 0.905884
Ran for 224050 frames Cell Events: 6808 mean_circ: 0.905822
Ran for 224550 frames Cell Events: 6830 mean_circ: 0.90585
Ran for 225050 frames Cell Events: 6841 mean_circ: 0.905866
Ran for 225550 frames Cell Events: 6847 mean_circ: 0.905875
Ran for 226050 frames Cell Events: 6860 mean_circ: 0.90582
Ran for 226550 frames Cell Events: 6874 mean_circ: 0.905833
Ran for 227050 frames Cell Events: 6896 mean_circ: 0.905853
Ran for 227550 frames Cell Events: 6916 mean_circ: 0.905928
Ran for 228050 frames Cell Events: 6931 mean_circ: 0.905941
Ran for 228550 frames Cell Events: 6941 mean_circ: 0.905937
Ran for 229050 frames Cell Events: 6956 mean_circ: 0.90594
Ran for 229550 frames Cell Events: 6974 mean_circ: 0.905854
Ran for 230050 frames Cell Events: 6996 mean_circ: 0.905901
Ran for 231050 frames Cell Events: 7032 mean_circ: 0.905874
Ran for 232050 frames Cell Events: 7065 mean_circ: 0.905839
Ran for 233050 frames Cell Events: 7096 mean_circ: 0.905772
Ran for 233550 frames Cell Events: 7112 mean_circ: 0.905825
Ran for 234550 frames Cell Events: 7141 mean_circ: 0.905877
Ran for 235050 frames Cell Events: 7164 mean_circ: 0.905888
Ran for 235550 frames Cell Events: 7182 mean_circ: 0.905857
Ran for 237550 frames Cell Events: 7239 mean_circ: 0.905866
Ran for 239550 frames Cell Events: 7319 mean_circ: 0.905782
Ran for 240050 frames Cell Events: 7335 mean_circ: 0.905747
Ran for 240550 frames Cell Events: 7355 mean_circ: 0.905756
Ran for 241550 frames Cell Events: 7405 mean_circ: 0.905773
Ran for 242050 frames Cell Events: 7423 mean_circ: 0.905833
Ran for 242550 frames Cell Events: 7444 mean_circ: 0.905864
Ran for 243050 frames Cell Events: 7453 mean_circ: 0.905878
Ran for 243550 frames Cell Events: 7468 mean_circ: 0.905875
Ran for 245050 frames Cell Events: 7502 mean_circ: 0.905828
Ran for 245550 frames Cell Events: 7514 mean_circ: 0.905856
Ran for 246050 frames Cell Events: 7530 mean_circ: 0.905888
Ran for 246550 frames Cell Events: 7543 mean_circ: 0.90582
Ran for 247050 frames Cell Events: 7559 mean_circ: 0.905751
Ran for 247550 frames Cell Events: 7565 mean_circ: 0.905767
Ran for 248050 frames Cell Events: 7581 mean_circ: 0.905708
Ran for 249050 frames Cell Events: 7612 mean_circ: 0.905665
Ran for 250050 frames Cell Events: 7640 mean_circ: 0.905637
Ran for 251050 frames Cell Events: 7675 mean_circ: 0.905612
Ran for 251550 frames Cell Events: 7685 mean_circ: 0.905579
Ran for 252050 frames Cell Events: 7709 mean_circ: 0.905505
Ran for 253550 frames Cell Events: 7758 mean_circ: 0.905412
Ran for 254050 frames Cell Events: 7769 mean_circ: 0.905429
Ran for 254550 frames Cell Events: 7783 mean_circ: 0.90544
Ran for 255050 frames Cell Events: 7797 mean_circ: 0.905415
Ran for 256550 frames Cell Events: 7849 mean_circ: 0.905342
Ran for 258050 frames Cell Events: 7903 mean_circ: 0.905372
Ran for 258550 frames Cell Events: 7921 mean_circ: 0.905462
Ran for 260050 frames Cell Events: 7969 mean_circ: 0.905402
Ran for 261550 frames Cell Events: 8018 mean_circ: 0.905327
Ran for 263050 frames Cell Events: 8069 mean_circ: 0.905189
Ran for 263550 frames Cell Events: 8079 mean_circ: 0.905203
Ran for 264550 frames Cell Events: 8109 mean_circ: 0.905268
Ran for 265550 frames Cell Events: 8139 mean_circ: 0.905269
Ran for 266050 frames Cell Events: 8160 mean_circ: 0.905223
Ran for 266550 frames Cell Events: 8183 mean_circ: 0.90519
Ran for 267050 frames Cell Events: 8198 mean_circ: 0.905217
Ran for 267550 frames Cell Events: 8210 mean_circ: 0.905195
Ran for 268050 frames Cell Events: 8238 mean_circ: 0.905151
Ran for 268550 frames Cell Events: 8254 mean_circ: 0.90514
Ran for 269050 frames Cell Events: 8267 mean_circ: 0.905212
Ran for 269550 frames Cell Events: 8287 mean_circ: 0.905237
Ran for 270050 frames Cell Events: 8304 mean_circ: 0.905233
End this measurment..
Stopping time: 5.49781e+008
****************************************
Measurement finished
Mean Circularitiy: 0.90512
Mean Area: 781.912
Mean FL-1 intensity: 3743.36
Mean FL-2 intensity: 4401.18
Mean FL-3 intensity: 0
frames processed: 268298
Total Time: 135257ms
Number of Runs (new bg img) 0
Frames collected: 270355
Skipped frames: 44590
Rescued Frames: 2191477
Samples DAQ: 67588664
Samples written: 135177032
Samples per Channel: 67588516
FPS: 1998.83
Samples per second: 499706
Expected samples/s: 500000
Events: 8314
Events per second: 61.4684
jumped back (PMT): 5694
#Frames read, frequency
# 0 frames :360079
# 1 frames :162914
# 2 frames :5304
# 3 frames :1727
# 4 frames :785
# 5 frames :233
# 6 frames :2661
# 7 frames :2235
# 8 frames :1220
# 9 frames :633
# 10 frames :859
# 11 frames :433
# 12 frames :375
# 13 frames :269
# 14 frames :205
# 15 frames :187
# 16 frames :142
# 17 frames :108
# 18 frames :70
# 19 frames :36
# 20 frames :31
# 21 frames :33
# 22 frames :27
# 23 frames :24
# 24 frames :15
# 25 frames :14
# 26 frames :15
# 27 frames :4
# 28 frames :11
# 29 frames :3
# 30 frames :3
# 31 frames :9
# 32 frames :1
# 33 frames :4
# 34 frames :2
# 36 frames :1
# 37 frames :3
# 38 frames :2
# 39 frames :1
# 41 frames :1
# 43 frames :2
# 44 frames :1
# 49 frames :1
M1_para.ini
[General]
Measurement Number = 1
Flow Rate [ul/s] = 0.04
Region = "Channel"
Channel width = 20
Current LED = 100
[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0
#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 25.0298
parameters.txt
#fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 300
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0