Click on the log titles below to expand the log entries present in this DC resource.
dclab-export_2024-07-09_19.01.17
{"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc
{
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "58c0de110e439cf8aa336e97264644d5",
"name": "M1_20170521_1707_dsRNA_A6_faster.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-09",
"time": "17:01:43"
}
}
dclab-tdms2rtdc-warnings
LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 7975 (max 7976) in '7975'.
FG_Config.mcf
[Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;
[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;
[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini
[Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6
[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini
[EVENT LOG] fRT-DC V1.06170206
Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 25.0578
Start pulse train for BG Aquisition..
Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 5.4987e+008
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 550 frames Cell Events: 15 mean_circ: 0.902162
Ran for 1050 frames Cell Events: 39 mean_circ: 0.896811
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Ran for 92550 frames Cell Events: 3136 mean_circ: 0.886621
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Ran for 100050 frames Cell Events: 3403 mean_circ: 0.887099
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Ran for 102550 frames Cell Events: 3483 mean_circ: 0.887031
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Ran for 122550 frames Cell Events: 4212 mean_circ: 0.886069
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Ran for 123550 frames Cell Events: 4262 mean_circ: 0.886103
Ran for 124050 frames Cell Events: 4290 mean_circ: 0.886297
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Ran for 126050 frames Cell Events: 4350 mean_circ: 0.886166
Ran for 126550 frames Cell Events: 4365 mean_circ: 0.886122
Ran for 127050 frames Cell Events: 4378 mean_circ: 0.886164
Ran for 127550 frames Cell Events: 4399 mean_circ: 0.886222
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Ran for 134050 frames Cell Events: 4613 mean_circ: 0.886547
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Ran for 197050 frames Cell Events: 6862 mean_circ: 0.886717
Ran for 197550 frames Cell Events: 6883 mean_circ: 0.886744
Ran for 198050 frames Cell Events: 6903 mean_circ: 0.886732
Ran for 199050 frames Cell Events: 6939 mean_circ: 0.886735
Ran for 199550 frames Cell Events: 6948 mean_circ: 0.886674
Ran for 200550 frames Cell Events: 6964 mean_circ: 0.886685
Ran for 201050 frames Cell Events: 6981 mean_circ: 0.886584
Ran for 201550 frames Cell Events: 6997 mean_circ: 0.886563
Ran for 202050 frames Cell Events: 7018 mean_circ: 0.886627
Ran for 202550 frames Cell Events: 7035 mean_circ: 0.88675
Ran for 203550 frames Cell Events: 7073 mean_circ: 0.886671
Ran for 204050 frames Cell Events: 7089 mean_circ: 0.88673
Ran for 204550 frames Cell Events: 7102 mean_circ: 0.886707
Ran for 205050 frames Cell Events: 7117 mean_circ: 0.886763
Ran for 205550 frames Cell Events: 7133 mean_circ: 0.886745
Ran for 206050 frames Cell Events: 7147 mean_circ: 0.88666
Ran for 206550 frames Cell Events: 7156 mean_circ: 0.886652
Ran for 207050 frames Cell Events: 7174 mean_circ: 0.886695
Ran for 207550 frames Cell Events: 7179 mean_circ: 0.886683
Ran for 209050 frames Cell Events: 7216 mean_circ: 0.886757
Ran for 209550 frames Cell Events: 7228 mean_circ: 0.886776
Ran for 210050 frames Cell Events: 7243 mean_circ: 0.886811
Ran for 211050 frames Cell Events: 7277 mean_circ: 0.886898
Ran for 211550 frames Cell Events: 7297 mean_circ: 0.886894
Ran for 212050 frames Cell Events: 7308 mean_circ: 0.886836
Ran for 212550 frames Cell Events: 7328 mean_circ: 0.88685
Ran for 213050 frames Cell Events: 7340 mean_circ: 0.886855
Ran for 213550 frames Cell Events: 7354 mean_circ: 0.88684
Ran for 214050 frames Cell Events: 7365 mean_circ: 0.886833
Ran for 215050 frames Cell Events: 7406 mean_circ: 0.886838
Ran for 215550 frames Cell Events: 7414 mean_circ: 0.886781
Ran for 216050 frames Cell Events: 7428 mean_circ: 0.886802
Ran for 216550 frames Cell Events: 7441 mean_circ: 0.886805
Ran for 217050 frames Cell Events: 7453 mean_circ: 0.886808
Ran for 217550 frames Cell Events: 7468 mean_circ: 0.886759
Ran for 218050 frames Cell Events: 7481 mean_circ: 0.886751
Ran for 218550 frames Cell Events: 7494 mean_circ: 0.886782
Ran for 219050 frames Cell Events: 7508 mean_circ: 0.886831
Ran for 219550 frames Cell Events: 7521 mean_circ: 0.886842
Ran for 220050 frames Cell Events: 7543 mean_circ: 0.886855
Ran for 220550 frames Cell Events: 7559 mean_circ: 0.886794
Ran for 222050 frames Cell Events: 7615 mean_circ: 0.886813
Ran for 222550 frames Cell Events: 7625 mean_circ: 0.886802
Ran for 223050 frames Cell Events: 7644 mean_circ: 0.886806
Ran for 224050 frames Cell Events: 7665 mean_circ: 0.88678
Ran for 224550 frames Cell Events: 7676 mean_circ: 0.886766
Ran for 225050 frames Cell Events: 7692 mean_circ: 0.886743
Ran for 225550 frames Cell Events: 7705 mean_circ: 0.886764
Ran for 226050 frames Cell Events: 7723 mean_circ: 0.886794
Ran for 226550 frames Cell Events: 7752 mean_circ: 0.88678
Ran for 227550 frames Cell Events: 7785 mean_circ: 0.886688
Ran for 228050 frames Cell Events: 7799 mean_circ: 0.886682
Ran for 229050 frames Cell Events: 7832 mean_circ: 0.886676
Ran for 230050 frames Cell Events: 7888 mean_circ: 0.886781
Ran for 230550 frames Cell Events: 7915 mean_circ: 0.886821
Ran for 231050 frames Cell Events: 7931 mean_circ: 0.886833
Ran for 231550 frames Cell Events: 7947 mean_circ: 0.886795
Ran for 232050 frames Cell Events: 7958 mean_circ: 0.886806
End this measurment..
Stopping time: 5.49987e+008
****************************************
Measurement finished
Mean Circularitiy: 0.886712
Mean Area: 798.34
Mean FL-1 intensity: 3596.59
Mean FL-2 intensity: 4827.04
Mean FL-3 intensity: 0
frames processed: 230362
Total Time: 116548ms
Number of Runs (new bg img) 0
Frames collected: 232930
Skipped frames: 47159
Rescued Frames: 2271940
Samples DAQ: 58232686
Samples written: 116465098
Samples per Channel: 58232549
FPS: 1998.57
Samples per second: 499642
Expected samples/s: 500000
Events: 7976
Events per second: 68.435
jumped back (PMT): 16
#Frames read, frequency
# 0 frames :315863
# 1 frames :133906
# 2 frames :4652
# 3 frames :1381
# 4 frames :643
# 5 frames :236
# 6 frames :3057
# 7 frames :1786
# 8 frames :1306
# 9 frames :594
# 10 frames :465
# 11 frames :330
# 12 frames :354
# 13 frames :226
# 14 frames :214
# 15 frames :158
# 16 frames :138
# 17 frames :95
# 18 frames :61
# 19 frames :50
# 20 frames :60
# 21 frames :44
# 22 frames :22
# 23 frames :21
# 24 frames :15
# 25 frames :17
# 26 frames :13
# 27 frames :8
# 28 frames :11
# 29 frames :8
# 30 frames :5
# 31 frames :6
# 32 frames :2
# 33 frames :4
# 34 frames :2
# 35 frames :2
# 36 frames :3
# 37 frames :1
# 40 frames :1
# 43 frames :1
# 48 frames :1
# 52 frames :1
# 53 frames :1
# 62 frames :1
# 68 frames :1
# 74 frames :1
# 92 frames :1
M1_para.ini
[General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100
[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0
#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 25.0578
parameters.txt
#fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0