Click on the log titles below to expand the log entries present in this DC resource.
dclab-export_2024-07-09_19.08.07
{"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc
{
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "dff5b5956ef9f7cd485e8e312e751fbe",
"name": "M1_20170521_1830_dsRNA_LZ_col.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-09",
"time": "17:08:34"
}
}
dclab-tdms2rtdc-warnings
LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 9999 (max 10000) in
'9999'.
FG_Config.mcf
[Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;
[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;
[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini
[Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6
[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini
[EVENT LOG] fRT-DC V1.06170206
Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 25.5338
Start pulse train for BG Aquisition..
Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 5.54869e+008
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 1050 frames Cell Events: 25 mean_circ: 0.943193
Ran for 1550 frames Cell Events: 36 mean_circ: 0.948156
Ran for 2050 frames Cell Events: 44 mean_circ: 0.952524
Ran for 3050 frames Cell Events: 73 mean_circ: 0.943694
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Ran for 4550 frames Cell Events: 118 mean_circ: 0.93825
Ran for 5550 frames Cell Events: 149 mean_circ: 0.9388
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Ran for 91050 frames Cell Events: 2802 mean_circ: 0.941223
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Ran for 92050 frames Cell Events: 2820 mean_circ: 0.941192
Ran for 92550 frames Cell Events: 2831 mean_circ: 0.941159
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Ran for 93550 frames Cell Events: 2850 mean_circ: 0.941233
Ran for 94050 frames Cell Events: 2860 mean_circ: 0.941314
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Ran for 95050 frames Cell Events: 2890 mean_circ: 0.941328
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Ran for 97050 frames Cell Events: 2963 mean_circ: 0.941266
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Ran for 98550 frames Cell Events: 3009 mean_circ: 0.941336
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Ran for 100550 frames Cell Events: 3059 mean_circ: 0.941359
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Ran for 102050 frames Cell Events: 3102 mean_circ: 0.941203
Ran for 102550 frames Cell Events: 3117 mean_circ: 0.941204
Ran for 103050 frames Cell Events: 3133 mean_circ: 0.941151
Ran for 103550 frames Cell Events: 3154 mean_circ: 0.941186
Ran for 104050 frames Cell Events: 3169 mean_circ: 0.941108
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Ran for 107550 frames Cell Events: 3278 mean_circ: 0.941125
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Ran for 121050 frames Cell Events: 3740 mean_circ: 0.940895
Ran for 122050 frames Cell Events: 3762 mean_circ: 0.940876
Ran for 123550 frames Cell Events: 3810 mean_circ: 0.940911
Ran for 124050 frames Cell Events: 3826 mean_circ: 0.940911
Ran for 127050 frames Cell Events: 3921 mean_circ: 0.940831
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Ran for 129550 frames Cell Events: 4008 mean_circ: 0.940952
Ran for 131050 frames Cell Events: 4051 mean_circ: 0.940828
Ran for 131550 frames Cell Events: 4066 mean_circ: 0.940854
Ran for 132050 frames Cell Events: 4082 mean_circ: 0.940833
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Ran for 141550 frames Cell Events: 4394 mean_circ: 0.94087
Ran for 142550 frames Cell Events: 4432 mean_circ: 0.940879
Ran for 143050 frames Cell Events: 4447 mean_circ: 0.940885
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Ran for 146550 frames Cell Events: 4562 mean_circ: 0.940984
Ran for 147050 frames Cell Events: 4584 mean_circ: 0.941012
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Ran for 182550 frames Cell Events: 5617 mean_circ: 0.941041
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Ran for 201550 frames Cell Events: 6248 mean_circ: 0.940793
Ran for 202050 frames Cell Events: 6267 mean_circ: 0.940776
Ran for 202550 frames Cell Events: 6278 mean_circ: 0.940755
Ran for 203550 frames Cell Events: 6309 mean_circ: 0.940831
Ran for 204050 frames Cell Events: 6329 mean_circ: 0.940749
Ran for 205050 frames Cell Events: 6358 mean_circ: 0.940749
Ran for 206050 frames Cell Events: 6398 mean_circ: 0.940727
Ran for 207050 frames Cell Events: 6429 mean_circ: 0.940754
Ran for 208050 frames Cell Events: 6464 mean_circ: 0.940696
Ran for 209050 frames Cell Events: 6498 mean_circ: 0.940664
Ran for 209550 frames Cell Events: 6518 mean_circ: 0.940665
Ran for 210050 frames Cell Events: 6526 mean_circ: 0.940684
Ran for 210550 frames Cell Events: 6550 mean_circ: 0.940704
Ran for 211050 frames Cell Events: 6562 mean_circ: 0.940701
Ran for 211550 frames Cell Events: 6577 mean_circ: 0.940652
Ran for 212050 frames Cell Events: 6597 mean_circ: 0.940653
Ran for 213050 frames Cell Events: 6629 mean_circ: 0.940602
Ran for 214050 frames Cell Events: 6676 mean_circ: 0.940563
Ran for 215050 frames Cell Events: 6703 mean_circ: 0.940614
Ran for 216550 frames Cell Events: 6745 mean_circ: 0.940687
Ran for 217050 frames Cell Events: 6762 mean_circ: 0.940675
Ran for 218050 frames Cell Events: 6814 mean_circ: 0.940708
Ran for 219050 frames Cell Events: 6856 mean_circ: 0.940765
Ran for 220550 frames Cell Events: 6902 mean_circ: 0.940722
Ran for 221050 frames Cell Events: 6915 mean_circ: 0.940688
Ran for 222050 frames Cell Events: 6936 mean_circ: 0.940719
Ran for 222550 frames Cell Events: 6954 mean_circ: 0.940744
Ran for 223550 frames Cell Events: 6985 mean_circ: 0.940666
Ran for 224550 frames Cell Events: 7012 mean_circ: 0.940628
Ran for 225050 frames Cell Events: 7030 mean_circ: 0.940643
Ran for 227050 frames Cell Events: 7086 mean_circ: 0.940748
Ran for 228050 frames Cell Events: 7117 mean_circ: 0.940711
Ran for 228550 frames Cell Events: 7130 mean_circ: 0.940734
Ran for 229050 frames Cell Events: 7150 mean_circ: 0.940774
Ran for 229550 frames Cell Events: 7165 mean_circ: 0.940792
Ran for 230050 frames Cell Events: 7179 mean_circ: 0.940793
Ran for 230550 frames Cell Events: 7194 mean_circ: 0.940818
Ran for 231050 frames Cell Events: 7207 mean_circ: 0.940823
Ran for 231550 frames Cell Events: 7214 mean_circ: 0.940813
Ran for 232050 frames Cell Events: 7228 mean_circ: 0.940834
Ran for 232550 frames Cell Events: 7241 mean_circ: 0.940807
Ran for 233550 frames Cell Events: 7273 mean_circ: 0.940825
Ran for 234050 frames Cell Events: 7291 mean_circ: 0.940838
Ran for 234550 frames Cell Events: 7308 mean_circ: 0.940849
Ran for 235050 frames Cell Events: 7324 mean_circ: 0.940832
Ran for 235550 frames Cell Events: 7346 mean_circ: 0.94081
Ran for 236550 frames Cell Events: 7378 mean_circ: 0.940737
Ran for 237550 frames Cell Events: 7430 mean_circ: 0.940742
Ran for 238550 frames Cell Events: 7462 mean_circ: 0.940658
Ran for 239050 frames Cell Events: 7471 mean_circ: 0.940665
Ran for 240050 frames Cell Events: 7514 mean_circ: 0.940677
Ran for 241050 frames Cell Events: 7564 mean_circ: 0.94064
Ran for 241550 frames Cell Events: 7581 mean_circ: 0.940662
Ran for 242050 frames Cell Events: 7599 mean_circ: 0.940712
Ran for 242550 frames Cell Events: 7616 mean_circ: 0.94069
Ran for 244050 frames Cell Events: 7665 mean_circ: 0.94071
Ran for 245550 frames Cell Events: 7722 mean_circ: 0.940714
Ran for 246050 frames Cell Events: 7752 mean_circ: 0.940764
Ran for 246550 frames Cell Events: 7767 mean_circ: 0.940751
Ran for 248550 frames Cell Events: 7838 mean_circ: 0.940747
Ran for 249050 frames Cell Events: 7858 mean_circ: 0.940731
Ran for 249550 frames Cell Events: 7881 mean_circ: 0.940746
Ran for 250050 frames Cell Events: 7902 mean_circ: 0.940729
Ran for 250550 frames Cell Events: 7927 mean_circ: 0.940776
Ran for 252050 frames Cell Events: 7975 mean_circ: 0.940803
Ran for 252550 frames Cell Events: 7996 mean_circ: 0.940782
Ran for 253050 frames Cell Events: 8011 mean_circ: 0.940785
Ran for 253550 frames Cell Events: 8030 mean_circ: 0.940829
Ran for 254050 frames Cell Events: 8048 mean_circ: 0.940858
Ran for 254550 frames Cell Events: 8071 mean_circ: 0.940869
Ran for 255050 frames Cell Events: 8086 mean_circ: 0.940868
Ran for 255550 frames Cell Events: 8108 mean_circ: 0.940886
Ran for 256550 frames Cell Events: 8131 mean_circ: 0.940883
Ran for 257050 frames Cell Events: 8146 mean_circ: 0.940845
Ran for 258050 frames Cell Events: 8182 mean_circ: 0.940867
Ran for 258550 frames Cell Events: 8204 mean_circ: 0.940889
Ran for 260550 frames Cell Events: 8260 mean_circ: 0.940857
Ran for 261050 frames Cell Events: 8270 mean_circ: 0.940881
Ran for 263550 frames Cell Events: 8362 mean_circ: 0.940912
Ran for 264050 frames Cell Events: 8386 mean_circ: 0.94089
Ran for 265050 frames Cell Events: 8427 mean_circ: 0.940855
Ran for 266050 frames Cell Events: 8474 mean_circ: 0.94094
Ran for 266550 frames Cell Events: 8484 mean_circ: 0.940957
Ran for 267050 frames Cell Events: 8496 mean_circ: 0.940961
Ran for 269050 frames Cell Events: 8567 mean_circ: 0.940924
Ran for 269550 frames Cell Events: 8584 mean_circ: 0.940932
Ran for 270050 frames Cell Events: 8592 mean_circ: 0.940938
Ran for 271550 frames Cell Events: 8652 mean_circ: 0.940909
Ran for 272050 frames Cell Events: 8670 mean_circ: 0.940904
Ran for 272550 frames Cell Events: 8685 mean_circ: 0.940895
Ran for 273550 frames Cell Events: 8723 mean_circ: 0.940903
Ran for 274050 frames Cell Events: 8734 mean_circ: 0.940913
Ran for 274550 frames Cell Events: 8752 mean_circ: 0.940893
Ran for 275550 frames Cell Events: 8782 mean_circ: 0.940891
Ran for 276050 frames Cell Events: 8792 mean_circ: 0.940857
Ran for 276550 frames Cell Events: 8805 mean_circ: 0.940879
Ran for 277050 frames Cell Events: 8819 mean_circ: 0.940894
Ran for 277550 frames Cell Events: 8837 mean_circ: 0.940899
Ran for 278050 frames Cell Events: 8849 mean_circ: 0.940902
Ran for 279050 frames Cell Events: 8881 mean_circ: 0.940882
Ran for 279550 frames Cell Events: 8893 mean_circ: 0.940864
Ran for 280050 frames Cell Events: 8908 mean_circ: 0.940826
Ran for 280550 frames Cell Events: 8923 mean_circ: 0.940842
Ran for 281550 frames Cell Events: 8957 mean_circ: 0.940827
Ran for 282550 frames Cell Events: 8991 mean_circ: 0.940778
Ran for 283050 frames Cell Events: 9016 mean_circ: 0.940778
Ran for 283550 frames Cell Events: 9036 mean_circ: 0.940799
Ran for 284050 frames Cell Events: 9070 mean_circ: 0.940763
Ran for 284550 frames Cell Events: 9088 mean_circ: 0.940761
Ran for 285050 frames Cell Events: 9107 mean_circ: 0.940739
Ran for 286050 frames Cell Events: 9153 mean_circ: 0.940726
Ran for 286550 frames Cell Events: 9167 mean_circ: 0.940742
Ran for 287550 frames Cell Events: 9212 mean_circ: 0.940729
Ran for 288050 frames Cell Events: 9232 mean_circ: 0.940717
Ran for 289550 frames Cell Events: 9301 mean_circ: 0.940726
Ran for 290050 frames Cell Events: 9312 mean_circ: 0.940763
Ran for 291050 frames Cell Events: 9358 mean_circ: 0.940775
Ran for 291550 frames Cell Events: 9370 mean_circ: 0.940781
Ran for 292050 frames Cell Events: 9393 mean_circ: 0.940793
Ran for 292550 frames Cell Events: 9410 mean_circ: 0.940804
Ran for 293050 frames Cell Events: 9423 mean_circ: 0.940798
Ran for 293550 frames Cell Events: 9446 mean_circ: 0.940844
Ran for 294050 frames Cell Events: 9469 mean_circ: 0.940841
Ran for 295050 frames Cell Events: 9510 mean_circ: 0.94082
Ran for 296050 frames Cell Events: 9539 mean_circ: 0.940783
Ran for 296550 frames Cell Events: 9558 mean_circ: 0.940775
Ran for 297550 frames Cell Events: 9586 mean_circ: 0.940782
Ran for 298550 frames Cell Events: 9615 mean_circ: 0.940782
Ran for 300050 frames Cell Events: 9680 mean_circ: 0.940731
Ran for 300550 frames Cell Events: 9695 mean_circ: 0.940757
Ran for 301050 frames Cell Events: 9714 mean_circ: 0.94075
Ran for 302050 frames Cell Events: 9751 mean_circ: 0.940772
Ran for 302550 frames Cell Events: 9765 mean_circ: 0.940785
Ran for 303550 frames Cell Events: 9793 mean_circ: 0.940838
Ran for 304050 frames Cell Events: 9811 mean_circ: 0.940852
Ran for 305050 frames Cell Events: 9841 mean_circ: 0.940851
Ran for 305550 frames Cell Events: 9856 mean_circ: 0.940845
Ran for 307050 frames Cell Events: 9939 mean_circ: 0.940837
Ran for 307550 frames Cell Events: 9958 mean_circ: 0.940817
End this measurment.
because stop condition (time/nr of cells) was met
Stopping time: 5.55023e+008
****************************************
Measurement finished
Mean Circularitiy: 0.940786
Mean Area: 625.475
Mean FL-1 intensity: 2938.44
Mean FL-2 intensity: 4245.41
Mean FL-3 intensity: 0
frames processed: 308118
Total Time: 154324ms
Number of Runs (new bg img) 0
Frames collected: 308470
Skipped frames: 72142
Rescued Frames: 3595096
Samples DAQ: 77118085
Samples written: 154234752
Samples per Channel: 77117376
FPS: 1998.84
Samples per second: 499710
Expected samples/s: 500000
Events: 10000
Events per second: 64.7987
jumped back (PMT): 5695
#Frames read, frequency
# 0 frames :437067
# 1 frames :158252
# 2 frames :5647
# 3 frames :2321
# 4 frames :1399
# 5 frames :616
# 6 frames :1441
# 7 frames :2241
# 8 frames :1406
# 9 frames :958
# 10 frames :1129
# 11 frames :894
# 12 frames :661
# 13 frames :532
# 14 frames :381
# 15 frames :344
# 16 frames :272
# 17 frames :257
# 18 frames :244
# 19 frames :147
# 20 frames :109
# 21 frames :75
# 22 frames :67
# 23 frames :56
# 24 frames :56
# 25 frames :43
# 26 frames :34
# 27 frames :28
# 28 frames :28
# 29 frames :26
# 30 frames :18
# 31 frames :27
# 32 frames :17
# 33 frames :6
# 34 frames :6
# 35 frames :10
# 36 frames :7
# 37 frames :5
# 38 frames :6
# 39 frames :5
# 41 frames :3
# 42 frames :1
# 43 frames :4
# 44 frames :2
# 46 frames :2
# 48 frames :2
# 49 frames :2
# 52 frames :1
# 54 frames :1
# 59 frames :1
# 61 frames :2
# 65 frames :1
M1_para.ini
[General]
Measurement Number = 1
Flow Rate [ul/s] = 0.04
Region = "Channel"
Channel width = 20
Current LED = 100
[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0
#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 25.5338
parameters.txt
#fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 300
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0