Click on the log titles below to expand the log entries present in this DC resource.
dclab-export_2024-07-09_19.08.41
{"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc
{
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "9b9b4242669b1b0fbde64892c9ba57c1",
"name": "M1_20170521_1836_dsRNA_LZ_faster.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-09",
"time": "17:09:05"
}
}
dclab-tdms2rtdc-warnings
LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 9363 (max 9364) in '9363'.
FG_Config.mcf
[Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;
[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;
[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini
[Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6
[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini
[EVENT LOG] fRT-DC V1.06170206
Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 25.5755
Start pulse train for BG Aquisition..
Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 5.55214e+008
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 550 frames Cell Events: 16 mean_circ: 0.897471
Ran for 1050 frames Cell Events: 51 mean_circ: 0.897471
Ran for 1550 frames Cell Events: 67 mean_circ: 0.912891
Ran for 2050 frames Cell Events: 84 mean_circ: 0.915589
Ran for 2550 frames Cell Events: 105 mean_circ: 0.918433
Ran for 3050 frames Cell Events: 121 mean_circ: 0.919347
Ran for 3550 frames Cell Events: 145 mean_circ: 0.919933
Ran for 5050 frames Cell Events: 203 mean_circ: 0.920832
Ran for 6050 frames Cell Events: 241 mean_circ: 0.921868
Ran for 6550 frames Cell Events: 255 mean_circ: 0.922055
Ran for 9050 frames Cell Events: 347 mean_circ: 0.921844
Ran for 9550 frames Cell Events: 363 mean_circ: 0.922123
Ran for 10050 frames Cell Events: 381 mean_circ: 0.921746
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Ran for 12550 frames Cell Events: 465 mean_circ: 0.922888
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Ran for 15050 frames Cell Events: 579 mean_circ: 0.924046
Ran for 16050 frames Cell Events: 627 mean_circ: 0.922329
Ran for 16550 frames Cell Events: 647 mean_circ: 0.922423
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Ran for 76550 frames Cell Events: 2851 mean_circ: 0.925911
Ran for 77050 frames Cell Events: 2872 mean_circ: 0.925893
Ran for 77550 frames Cell Events: 2887 mean_circ: 0.925873
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Ran for 82050 frames Cell Events: 3063 mean_circ: 0.926159
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Ran for 83050 frames Cell Events: 3118 mean_circ: 0.926286
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Ran for 85550 frames Cell Events: 3223 mean_circ: 0.926461
Ran for 86050 frames Cell Events: 3249 mean_circ: 0.92646
Ran for 86550 frames Cell Events: 3262 mean_circ: 0.926427
Ran for 87050 frames Cell Events: 3273 mean_circ: 0.926451
Ran for 88050 frames Cell Events: 3307 mean_circ: 0.926359
Ran for 88550 frames Cell Events: 3331 mean_circ: 0.926416
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Ran for 92050 frames Cell Events: 3489 mean_circ: 0.925949
Ran for 93050 frames Cell Events: 3530 mean_circ: 0.925851
Ran for 93550 frames Cell Events: 3548 mean_circ: 0.925918
Ran for 94550 frames Cell Events: 3591 mean_circ: 0.926012
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Ran for 95550 frames Cell Events: 3628 mean_circ: 0.925987
Ran for 96550 frames Cell Events: 3677 mean_circ: 0.925961
Ran for 97550 frames Cell Events: 3709 mean_circ: 0.926091
Ran for 98050 frames Cell Events: 3726 mean_circ: 0.926072
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Ran for 100050 frames Cell Events: 3809 mean_circ: 0.926331
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Ran for 102050 frames Cell Events: 3880 mean_circ: 0.926232
Ran for 103050 frames Cell Events: 3909 mean_circ: 0.926261
Ran for 103550 frames Cell Events: 3930 mean_circ: 0.926276
Ran for 104050 frames Cell Events: 3952 mean_circ: 0.926117
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Ran for 106050 frames Cell Events: 4034 mean_circ: 0.926269
Ran for 106550 frames Cell Events: 4050 mean_circ: 0.926379
Ran for 107050 frames Cell Events: 4068 mean_circ: 0.926303
Ran for 107550 frames Cell Events: 4104 mean_circ: 0.926348
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Ran for 111550 frames Cell Events: 4256 mean_circ: 0.926349
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Ran for 112550 frames Cell Events: 4298 mean_circ: 0.926341
Ran for 113050 frames Cell Events: 4315 mean_circ: 0.926282
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Ran for 120050 frames Cell Events: 4547 mean_circ: 0.926212
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Ran for 121050 frames Cell Events: 4587 mean_circ: 0.926145
Ran for 121550 frames Cell Events: 4605 mean_circ: 0.926181
Ran for 123550 frames Cell Events: 4678 mean_circ: 0.926028
Ran for 124050 frames Cell Events: 4693 mean_circ: 0.926065
Ran for 124550 frames Cell Events: 4716 mean_circ: 0.926068
Ran for 125050 frames Cell Events: 4723 mean_circ: 0.926065
Ran for 125550 frames Cell Events: 4745 mean_circ: 0.926077
Ran for 126550 frames Cell Events: 4779 mean_circ: 0.926094
Ran for 128550 frames Cell Events: 4870 mean_circ: 0.92603
Ran for 129050 frames Cell Events: 4893 mean_circ: 0.926017
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Ran for 131550 frames Cell Events: 4975 mean_circ: 0.925845
Ran for 132050 frames Cell Events: 4992 mean_circ: 0.925836
Ran for 133050 frames Cell Events: 5019 mean_circ: 0.925877
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Ran for 143550 frames Cell Events: 5429 mean_circ: 0.925687
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Ran for 174050 frames Cell Events: 6582 mean_circ: 0.925523
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Ran for 194050 frames Cell Events: 7306 mean_circ: 0.925351
Ran for 195050 frames Cell Events: 7344 mean_circ: 0.925305
Ran for 196550 frames Cell Events: 7425 mean_circ: 0.925306
Ran for 197550 frames Cell Events: 7468 mean_circ: 0.925306
Ran for 198550 frames Cell Events: 7502 mean_circ: 0.925339
Ran for 199050 frames Cell Events: 7533 mean_circ: 0.925292
Ran for 199550 frames Cell Events: 7550 mean_circ: 0.925326
Ran for 201550 frames Cell Events: 7617 mean_circ: 0.925347
Ran for 202050 frames Cell Events: 7631 mean_circ: 0.925325
Ran for 202550 frames Cell Events: 7653 mean_circ: 0.925287
Ran for 203550 frames Cell Events: 7680 mean_circ: 0.92531
Ran for 204050 frames Cell Events: 7697 mean_circ: 0.925292
Ran for 205050 frames Cell Events: 7743 mean_circ: 0.925267
Ran for 205550 frames Cell Events: 7762 mean_circ: 0.925288
Ran for 206050 frames Cell Events: 7785 mean_circ: 0.925318
Ran for 206550 frames Cell Events: 7803 mean_circ: 0.925279
Ran for 207050 frames Cell Events: 7831 mean_circ: 0.925259
Ran for 207550 frames Cell Events: 7856 mean_circ: 0.925288
Ran for 208550 frames Cell Events: 7898 mean_circ: 0.925292
Ran for 209050 frames Cell Events: 7912 mean_circ: 0.925294
Ran for 209550 frames Cell Events: 7923 mean_circ: 0.925278
Ran for 210050 frames Cell Events: 7941 mean_circ: 0.925304
Ran for 210550 frames Cell Events: 7956 mean_circ: 0.925306
Ran for 211550 frames Cell Events: 7985 mean_circ: 0.925298
Ran for 212050 frames Cell Events: 8005 mean_circ: 0.925291
Ran for 213550 frames Cell Events: 8082 mean_circ: 0.925302
Ran for 215550 frames Cell Events: 8152 mean_circ: 0.925305
Ran for 216050 frames Cell Events: 8178 mean_circ: 0.925313
Ran for 216550 frames Cell Events: 8199 mean_circ: 0.925251
Ran for 217050 frames Cell Events: 8218 mean_circ: 0.925266
Ran for 217550 frames Cell Events: 8232 mean_circ: 0.925262
Ran for 218050 frames Cell Events: 8248 mean_circ: 0.925254
Ran for 221050 frames Cell Events: 8346 mean_circ: 0.925304
Ran for 221550 frames Cell Events: 8361 mean_circ: 0.925329
Ran for 222050 frames Cell Events: 8379 mean_circ: 0.925307
Ran for 222550 frames Cell Events: 8399 mean_circ: 0.925278
Ran for 223050 frames Cell Events: 8423 mean_circ: 0.925306
Ran for 224550 frames Cell Events: 8471 mean_circ: 0.925327
Ran for 225550 frames Cell Events: 8510 mean_circ: 0.925349
Ran for 226050 frames Cell Events: 8528 mean_circ: 0.925327
Ran for 226550 frames Cell Events: 8551 mean_circ: 0.925342
Ran for 227050 frames Cell Events: 8570 mean_circ: 0.925367
Ran for 228550 frames Cell Events: 8624 mean_circ: 0.925404
Ran for 229050 frames Cell Events: 8646 mean_circ: 0.925408
Ran for 229550 frames Cell Events: 8660 mean_circ: 0.925336
Ran for 230050 frames Cell Events: 8683 mean_circ: 0.92537
Ran for 230550 frames Cell Events: 8700 mean_circ: 0.925339
Ran for 232550 frames Cell Events: 8787 mean_circ: 0.925314
Ran for 233550 frames Cell Events: 8832 mean_circ: 0.925338
Ran for 234050 frames Cell Events: 8843 mean_circ: 0.92536
Ran for 234550 frames Cell Events: 8861 mean_circ: 0.925367
Ran for 235050 frames Cell Events: 8880 mean_circ: 0.925366
Ran for 235550 frames Cell Events: 8903 mean_circ: 0.925379
Ran for 236050 frames Cell Events: 8917 mean_circ: 0.925372
Ran for 236550 frames Cell Events: 8928 mean_circ: 0.925388
Ran for 237050 frames Cell Events: 8943 mean_circ: 0.925398
Ran for 239550 frames Cell Events: 9031 mean_circ: 0.925373
Ran for 240050 frames Cell Events: 9042 mean_circ: 0.925355
Ran for 241050 frames Cell Events: 9094 mean_circ: 0.925266
Ran for 241550 frames Cell Events: 9124 mean_circ: 0.925292
Ran for 242050 frames Cell Events: 9147 mean_circ: 0.925244
Ran for 243050 frames Cell Events: 9188 mean_circ: 0.925223
Ran for 244550 frames Cell Events: 9227 mean_circ: 0.925161
Ran for 245050 frames Cell Events: 9239 mean_circ: 0.925155
Ran for 245550 frames Cell Events: 9256 mean_circ: 0.925127
Ran for 246550 frames Cell Events: 9302 mean_circ: 0.925181
Ran for 248050 frames Cell Events: 9344 mean_circ: 0.925207
Ran for 248550 frames Cell Events: 9360 mean_circ: 0.925168
End this measurment..
Stopping time: 5.55339e+008
****************************************
Measurement finished
Mean Circularitiy: 0.925163
Mean Area: 636.688
Mean FL-1 intensity: 2818.01
Mean FL-2 intensity: 4474.2
Mean FL-3 intensity: 0
frames processed: 246323
Total Time: 124432ms
Number of Runs (new bg img) 0
Frames collected: 248685
Skipped frames: 74504
Rescued Frames: 3690006
Samples DAQ: 62171265
Samples written: 124342304
Samples per Channel: 62171152
FPS: 1998.56
Samples per second: 499639
Expected samples/s: 500000
Events: 9364
Events per second: 75.2539
jumped back (PMT): 17
#Frames read, frequency
# 0 frames :345919
# 1 frames :132779
# 2 frames :5481
# 3 frames :1681
# 4 frames :852
# 5 frames :318
# 6 frames :2875
# 7 frames :2212
# 8 frames :1537
# 9 frames :813
# 10 frames :631
# 11 frames :375
# 12 frames :395
# 13 frames :286
# 14 frames :244
# 15 frames :196
# 16 frames :143
# 17 frames :128
# 18 frames :98
# 19 frames :54
# 20 frames :55
# 21 frames :52
# 22 frames :44
# 23 frames :32
# 24 frames :29
# 25 frames :12
# 26 frames :23
# 27 frames :10
# 28 frames :6
# 29 frames :11
# 30 frames :5
# 31 frames :3
# 32 frames :6
# 33 frames :10
# 34 frames :2
# 36 frames :3
# 37 frames :2
# 38 frames :1
# 39 frames :1
# 40 frames :1
# 41 frames :2
# 43 frames :1
# 44 frames :1
# 45 frames :1
# 48 frames :1
# 50 frames :1
# 58 frames :1
# 59 frames :1
# 60 frames :1
# 61 frames :1
# 68 frames :1
M1_para.ini
[General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100
[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0
#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 25.5755
parameters.txt
#fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0