Click on the log titles below to expand the log entries present in this DC resource.
dclab-export_2024-07-09_19.12.34
{"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc
{
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "0ea25f4d8c4db3a0c149d1c7debeb6c0",
"name": "M1_20170521_1923_dsRNA_L7_faster.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-09",
"time": "17:12:59"
}
}
dclab-tdms2rtdc-warnings
LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 10000 (max 10001) in
'10000'.
FG_Config.mcf
[Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;
[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;
[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini
[Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6
[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini
[EVENT LOG] fRT-DC V1.06170206
Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 25.8679
Start pulse train for BG Aquisition..
Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 5.58027e+008
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 1550 frames Cell Events: 105 mean_circ: 0.9285
Ran for 4050 frames Cell Events: 255 mean_circ: 0.931682
Ran for 5050 frames Cell Events: 336 mean_circ: 0.932336
Ran for 6050 frames Cell Events: 402 mean_circ: 0.929538
Ran for 6550 frames Cell Events: 426 mean_circ: 0.928965
Ran for 7050 frames Cell Events: 458 mean_circ: 0.928581
Ran for 7550 frames Cell Events: 487 mean_circ: 0.929614
Ran for 8050 frames Cell Events: 522 mean_circ: 0.930201
Ran for 8550 frames Cell Events: 553 mean_circ: 0.930471
Ran for 10550 frames Cell Events: 674 mean_circ: 0.930348
Ran for 11050 frames Cell Events: 712 mean_circ: 0.930188
Ran for 12050 frames Cell Events: 767 mean_circ: 0.930707
Ran for 13550 frames Cell Events: 853 mean_circ: 0.931398
Ran for 14550 frames Cell Events: 931 mean_circ: 0.931418
Ran for 15550 frames Cell Events: 983 mean_circ: 0.931196
Ran for 16550 frames Cell Events: 1033 mean_circ: 0.931427
Ran for 19550 frames Cell Events: 1186 mean_circ: 0.931689
Ran for 21050 frames Cell Events: 1290 mean_circ: 0.932111
Ran for 22050 frames Cell Events: 1366 mean_circ: 0.93219
Ran for 22550 frames Cell Events: 1393 mean_circ: 0.93217
Ran for 23550 frames Cell Events: 1476 mean_circ: 0.931989
Ran for 24550 frames Cell Events: 1537 mean_circ: 0.93234
Ran for 25550 frames Cell Events: 1599 mean_circ: 0.932351
Ran for 28050 frames Cell Events: 1771 mean_circ: 0.932637
Ran for 31550 frames Cell Events: 2007 mean_circ: 0.932549
Ran for 33050 frames Cell Events: 2113 mean_circ: 0.932467
Ran for 33550 frames Cell Events: 2140 mean_circ: 0.932624
Ran for 34050 frames Cell Events: 2172 mean_circ: 0.932581
Ran for 34550 frames Cell Events: 2189 mean_circ: 0.932399
Ran for 35550 frames Cell Events: 2254 mean_circ: 0.932443
Ran for 36050 frames Cell Events: 2284 mean_circ: 0.932577
Ran for 37050 frames Cell Events: 2350 mean_circ: 0.932605
Ran for 37550 frames Cell Events: 2394 mean_circ: 0.932577
Ran for 38050 frames Cell Events: 2411 mean_circ: 0.932594
Ran for 38550 frames Cell Events: 2435 mean_circ: 0.932584
Ran for 39550 frames Cell Events: 2490 mean_circ: 0.932646
Ran for 41050 frames Cell Events: 2581 mean_circ: 0.932375
Ran for 41550 frames Cell Events: 2611 mean_circ: 0.93244
Ran for 42050 frames Cell Events: 2640 mean_circ: 0.932567
Ran for 43050 frames Cell Events: 2696 mean_circ: 0.93284
Ran for 43550 frames Cell Events: 2732 mean_circ: 0.932944
Ran for 44050 frames Cell Events: 2758 mean_circ: 0.933014
Ran for 46050 frames Cell Events: 2878 mean_circ: 0.932994
Ran for 46550 frames Cell Events: 2888 mean_circ: 0.933011
Ran for 47050 frames Cell Events: 2909 mean_circ: 0.93311
Ran for 49050 frames Cell Events: 3030 mean_circ: 0.932965
Ran for 49550 frames Cell Events: 3055 mean_circ: 0.932944
Ran for 50550 frames Cell Events: 3115 mean_circ: 0.932969
Ran for 51050 frames Cell Events: 3142 mean_circ: 0.932905
Ran for 51550 frames Cell Events: 3167 mean_circ: 0.932968
Ran for 53050 frames Cell Events: 3276 mean_circ: 0.932862
Ran for 53550 frames Cell Events: 3305 mean_circ: 0.932868
Ran for 54550 frames Cell Events: 3364 mean_circ: 0.932871
Ran for 55050 frames Cell Events: 3386 mean_circ: 0.932915
Ran for 56050 frames Cell Events: 3444 mean_circ: 0.93303
Ran for 57050 frames Cell Events: 3497 mean_circ: 0.933137
Ran for 57550 frames Cell Events: 3533 mean_circ: 0.933136
Ran for 58050 frames Cell Events: 3584 mean_circ: 0.933236
Ran for 60050 frames Cell Events: 3723 mean_circ: 0.933528
Ran for 62050 frames Cell Events: 3828 mean_circ: 0.933606
Ran for 63050 frames Cell Events: 3908 mean_circ: 0.933606
Ran for 64050 frames Cell Events: 3955 mean_circ: 0.933375
Ran for 64550 frames Cell Events: 3984 mean_circ: 0.933395
Ran for 65050 frames Cell Events: 4012 mean_circ: 0.933351
Ran for 69550 frames Cell Events: 4308 mean_circ: 0.933495
Ran for 70050 frames Cell Events: 4337 mean_circ: 0.93351
Ran for 72050 frames Cell Events: 4474 mean_circ: 0.933452
Ran for 72550 frames Cell Events: 4509 mean_circ: 0.933531
Ran for 73550 frames Cell Events: 4580 mean_circ: 0.933548
Ran for 74050 frames Cell Events: 4612 mean_circ: 0.933635
Ran for 75550 frames Cell Events: 4700 mean_circ: 0.933446
Ran for 76050 frames Cell Events: 4731 mean_circ: 0.933411
Ran for 77550 frames Cell Events: 4856 mean_circ: 0.933488
Ran for 79050 frames Cell Events: 4960 mean_circ: 0.933375
Ran for 81550 frames Cell Events: 5110 mean_circ: 0.933212
Ran for 82550 frames Cell Events: 5159 mean_circ: 0.933213
Ran for 84550 frames Cell Events: 5280 mean_circ: 0.933239
Ran for 85550 frames Cell Events: 5320 mean_circ: 0.933277
Ran for 86050 frames Cell Events: 5344 mean_circ: 0.933306
Ran for 90550 frames Cell Events: 5621 mean_circ: 0.933362
Ran for 91050 frames Cell Events: 5635 mean_circ: 0.933375
Ran for 92050 frames Cell Events: 5696 mean_circ: 0.933299
Ran for 93050 frames Cell Events: 5730 mean_circ: 0.933308
Ran for 94050 frames Cell Events: 5797 mean_circ: 0.933088
Ran for 95050 frames Cell Events: 5839 mean_circ: 0.933138
Ran for 95550 frames Cell Events: 5878 mean_circ: 0.933108
Ran for 96050 frames Cell Events: 5905 mean_circ: 0.933109
Ran for 97550 frames Cell Events: 6003 mean_circ: 0.933169
Ran for 98050 frames Cell Events: 6053 mean_circ: 0.93318
Ran for 98550 frames Cell Events: 6072 mean_circ: 0.933272
Ran for 99050 frames Cell Events: 6097 mean_circ: 0.933292
Ran for 99550 frames Cell Events: 6119 mean_circ: 0.933308
Ran for 100550 frames Cell Events: 6188 mean_circ: 0.93324
Ran for 101050 frames Cell Events: 6216 mean_circ: 0.933214
Ran for 102550 frames Cell Events: 6312 mean_circ: 0.933158
Ran for 103050 frames Cell Events: 6341 mean_circ: 0.933158
Ran for 103550 frames Cell Events: 6364 mean_circ: 0.933198
Ran for 104550 frames Cell Events: 6416 mean_circ: 0.933193
Ran for 105050 frames Cell Events: 6443 mean_circ: 0.933215
Ran for 105550 frames Cell Events: 6463 mean_circ: 0.933191
Ran for 108050 frames Cell Events: 6627 mean_circ: 0.933147
Ran for 110050 frames Cell Events: 6755 mean_circ: 0.933119
Ran for 113550 frames Cell Events: 6959 mean_circ: 0.933234
Ran for 114550 frames Cell Events: 7000 mean_circ: 0.933209
Ran for 116050 frames Cell Events: 7082 mean_circ: 0.933123
Ran for 116550 frames Cell Events: 7105 mean_circ: 0.933027
Ran for 117050 frames Cell Events: 7129 mean_circ: 0.933088
Ran for 117550 frames Cell Events: 7166 mean_circ: 0.933026
Ran for 118550 frames Cell Events: 7219 mean_circ: 0.933021
Ran for 119550 frames Cell Events: 7306 mean_circ: 0.933036
Ran for 120050 frames Cell Events: 7332 mean_circ: 0.933043
Ran for 120550 frames Cell Events: 7357 mean_circ: 0.933081
Ran for 121050 frames Cell Events: 7374 mean_circ: 0.933074
Ran for 123550 frames Cell Events: 7566 mean_circ: 0.933002
Ran for 125050 frames Cell Events: 7655 mean_circ: 0.932986
Ran for 126050 frames Cell Events: 7721 mean_circ: 0.933022
Ran for 126550 frames Cell Events: 7744 mean_circ: 0.93308
Ran for 127050 frames Cell Events: 7778 mean_circ: 0.933115
Ran for 127550 frames Cell Events: 7798 mean_circ: 0.933109
Ran for 131550 frames Cell Events: 8088 mean_circ: 0.933045
Ran for 132550 frames Cell Events: 8152 mean_circ: 0.933101
Ran for 133050 frames Cell Events: 8188 mean_circ: 0.933153
Ran for 133550 frames Cell Events: 8213 mean_circ: 0.933173
Ran for 134050 frames Cell Events: 8244 mean_circ: 0.933201
Ran for 135050 frames Cell Events: 8290 mean_circ: 0.933246
Ran for 135550 frames Cell Events: 8319 mean_circ: 0.933233
Ran for 136050 frames Cell Events: 8349 mean_circ: 0.933234
Ran for 136550 frames Cell Events: 8379 mean_circ: 0.933257
Ran for 137550 frames Cell Events: 8451 mean_circ: 0.933188
Ran for 139050 frames Cell Events: 8536 mean_circ: 0.933238
Ran for 141050 frames Cell Events: 8623 mean_circ: 0.933224
Ran for 141550 frames Cell Events: 8643 mean_circ: 0.933185
Ran for 142550 frames Cell Events: 8690 mean_circ: 0.93315
Ran for 144050 frames Cell Events: 8800 mean_circ: 0.933095
Ran for 144550 frames Cell Events: 8854 mean_circ: 0.933095
Ran for 145550 frames Cell Events: 8914 mean_circ: 0.933182
Ran for 146550 frames Cell Events: 8996 mean_circ: 0.933161
Ran for 147050 frames Cell Events: 9024 mean_circ: 0.933077
Ran for 150050 frames Cell Events: 9230 mean_circ: 0.932951
Ran for 150550 frames Cell Events: 9252 mean_circ: 0.93297
Ran for 152050 frames Cell Events: 9355 mean_circ: 0.932827
Ran for 154050 frames Cell Events: 9471 mean_circ: 0.932774
Ran for 154550 frames Cell Events: 9507 mean_circ: 0.932748
Ran for 156050 frames Cell Events: 9626 mean_circ: 0.932738
Ran for 156550 frames Cell Events: 9646 mean_circ: 0.932763
Ran for 157050 frames Cell Events: 9676 mean_circ: 0.932733
Ran for 159050 frames Cell Events: 9793 mean_circ: 0.932666
Ran for 159550 frames Cell Events: 9811 mean_circ: 0.932649
Ran for 160050 frames Cell Events: 9837 mean_circ: 0.932659
Ran for 161050 frames Cell Events: 9898 mean_circ: 0.932566
Ran for 161550 frames Cell Events: 9936 mean_circ: 0.932592
End this measurment.
because stop condition (time/nr of cells) was met
Stopping time: 5.58108e+008
****************************************
Measurement finished
Mean Circularitiy: 0.93259
Mean Area: 600.178
Mean FL-1 intensity: 3181.15
Mean FL-2 intensity: 4095.41
Mean FL-3 intensity: 0
frames processed: 161097
Total Time: 81305.5ms
Number of Runs (new bg img) 0
Frames collected: 162437
Skipped frames: 87761
Rescued Frames: 4391025
Samples DAQ: 40610485
Samples written: 81218432
Samples per Channel: 40609216
FPS: 1997.86
Samples per second: 499464
Expected samples/s: 500000
Events: 10001
Events per second: 123.005
jumped back (PMT): 21
#Frames read, frequency
# 0 frames :250033
# 1 frames :61269
# 2 frames :3179
# 3 frames :1011
# 4 frames :482
# 5 frames :229
# 6 frames :2357
# 7 frames :1526
# 8 frames :1249
# 9 frames :628
# 10 frames :455
# 11 frames :273
# 12 frames :232
# 13 frames :197
# 14 frames :256
# 15 frames :238
# 16 frames :165
# 17 frames :144
# 18 frames :111
# 19 frames :76
# 20 frames :81
# 21 frames :73
# 22 frames :85
# 23 frames :62
# 24 frames :38
# 25 frames :44
# 26 frames :35
# 27 frames :26
# 28 frames :22
# 29 frames :16
# 30 frames :25
# 31 frames :14
# 32 frames :13
# 33 frames :24
# 34 frames :14
# 35 frames :11
# 36 frames :15
# 37 frames :8
# 38 frames :12
# 39 frames :9
# 40 frames :13
# 41 frames :10
# 42 frames :2
# 43 frames :4
# 44 frames :1
# 45 frames :5
# 46 frames :3
# 47 frames :5
# 48 frames :6
# 49 frames :1
# 50 frames :2
# 51 frames :2
# 52 frames :2
# 54 frames :3
# 55 frames :1
# 58 frames :1
# 59 frames :1
# 62 frames :2
# 66 frames :1
# 73 frames :1
M1_para.ini
[General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100
[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0
#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 25.8679
parameters.txt
#fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0