Click on the log titles below to expand the log entries present in this DC resource.
dclab-export_2024-07-09_19.13.33
{"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc
{
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "44f8cd67f5e6a470e5d54369c96f5ab5",
"name": "M1_20170521_1933_dsRNA_L2_faster.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-09",
"time": "17:13:54"
}
}
dclab-tdms2rtdc-warnings
LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8036 (max 8037) in '8036'.
FG_Config.mcf
[Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;
[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;
[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini
[Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6
[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini
[EVENT LOG] fRT-DC V1.06170206
Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 25.8807
Start pulse train for BG Aquisition..
Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 5.58677e+008
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 550 frames Cell Events: 18 mean_circ: 0.921801
Ran for 1050 frames Cell Events: 31 mean_circ: 0.928566
Ran for 2550 frames Cell Events: 94 mean_circ: 0.924781
Ran for 3050 frames Cell Events: 108 mean_circ: 0.925661
Ran for 3550 frames Cell Events: 124 mean_circ: 0.926018
Ran for 4050 frames Cell Events: 138 mean_circ: 0.925969
Ran for 4550 frames Cell Events: 151 mean_circ: 0.928609
Ran for 5050 frames Cell Events: 164 mean_circ: 0.926666
Ran for 5550 frames Cell Events: 185 mean_circ: 0.92683
Ran for 7050 frames Cell Events: 226 mean_circ: 0.926569
Ran for 7550 frames Cell Events: 259 mean_circ: 0.926807
Ran for 8050 frames Cell Events: 280 mean_circ: 0.924004
Ran for 9050 frames Cell Events: 317 mean_circ: 0.924947
Ran for 9550 frames Cell Events: 331 mean_circ: 0.925219
Ran for 10050 frames Cell Events: 353 mean_circ: 0.924991
Ran for 10550 frames Cell Events: 371 mean_circ: 0.923871
Ran for 11050 frames Cell Events: 385 mean_circ: 0.923877
Ran for 11550 frames Cell Events: 394 mean_circ: 0.923702
Ran for 12050 frames Cell Events: 410 mean_circ: 0.923762
Ran for 12550 frames Cell Events: 426 mean_circ: 0.924904
Ran for 14050 frames Cell Events: 471 mean_circ: 0.923585
Ran for 15050 frames Cell Events: 495 mean_circ: 0.923449
Ran for 15550 frames Cell Events: 509 mean_circ: 0.92258
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Ran for 35550 frames Cell Events: 1176 mean_circ: 0.923887
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Ran for 38050 frames Cell Events: 1272 mean_circ: 0.923825
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Ran for 63550 frames Cell Events: 2289 mean_circ: 0.92378
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Ran for 65550 frames Cell Events: 2360 mean_circ: 0.923898
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Ran for 68050 frames Cell Events: 2451 mean_circ: 0.924187
Ran for 70050 frames Cell Events: 2532 mean_circ: 0.923921
Ran for 70550 frames Cell Events: 2550 mean_circ: 0.923799
Ran for 71550 frames Cell Events: 2589 mean_circ: 0.923792
Ran for 72050 frames Cell Events: 2614 mean_circ: 0.923744
Ran for 72550 frames Cell Events: 2634 mean_circ: 0.923703
Ran for 73050 frames Cell Events: 2659 mean_circ: 0.923718
Ran for 73550 frames Cell Events: 2679 mean_circ: 0.923808
Ran for 74050 frames Cell Events: 2700 mean_circ: 0.923796
Ran for 74550 frames Cell Events: 2715 mean_circ: 0.923842
Ran for 75050 frames Cell Events: 2730 mean_circ: 0.923655
Ran for 75550 frames Cell Events: 2747 mean_circ: 0.923743
Ran for 76550 frames Cell Events: 2793 mean_circ: 0.923893
Ran for 77050 frames Cell Events: 2817 mean_circ: 0.923823
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Ran for 78550 frames Cell Events: 2871 mean_circ: 0.923932
Ran for 80550 frames Cell Events: 2952 mean_circ: 0.923909
Ran for 81050 frames Cell Events: 2967 mean_circ: 0.923914
Ran for 81550 frames Cell Events: 2983 mean_circ: 0.923987
Ran for 82050 frames Cell Events: 3008 mean_circ: 0.923865
Ran for 83050 frames Cell Events: 3041 mean_circ: 0.924061
Ran for 84050 frames Cell Events: 3072 mean_circ: 0.924093
Ran for 84550 frames Cell Events: 3081 mean_circ: 0.924049
Ran for 85550 frames Cell Events: 3121 mean_circ: 0.924127
Ran for 86050 frames Cell Events: 3139 mean_circ: 0.924032
Ran for 86550 frames Cell Events: 3174 mean_circ: 0.924076
Ran for 87050 frames Cell Events: 3195 mean_circ: 0.9239
Ran for 88050 frames Cell Events: 3244 mean_circ: 0.923984
Ran for 88550 frames Cell Events: 3257 mean_circ: 0.923924
Ran for 90050 frames Cell Events: 3313 mean_circ: 0.923989
Ran for 90550 frames Cell Events: 3334 mean_circ: 0.924109
Ran for 91050 frames Cell Events: 3350 mean_circ: 0.924102
Ran for 92050 frames Cell Events: 3390 mean_circ: 0.923999
Ran for 93550 frames Cell Events: 3427 mean_circ: 0.923933
Ran for 94050 frames Cell Events: 3439 mean_circ: 0.923953
Ran for 94550 frames Cell Events: 3453 mean_circ: 0.923943
Ran for 95050 frames Cell Events: 3472 mean_circ: 0.923894
Ran for 97050 frames Cell Events: 3553 mean_circ: 0.923815
Ran for 98050 frames Cell Events: 3596 mean_circ: 0.923792
Ran for 98550 frames Cell Events: 3613 mean_circ: 0.923819
Ran for 99050 frames Cell Events: 3632 mean_circ: 0.923774
Ran for 99550 frames Cell Events: 3652 mean_circ: 0.923818
Ran for 100550 frames Cell Events: 3691 mean_circ: 0.923797
Ran for 101050 frames Cell Events: 3715 mean_circ: 0.923888
Ran for 102050 frames Cell Events: 3756 mean_circ: 0.92401
Ran for 102550 frames Cell Events: 3768 mean_circ: 0.924085
Ran for 103050 frames Cell Events: 3788 mean_circ: 0.924152
Ran for 103550 frames Cell Events: 3814 mean_circ: 0.924092
Ran for 104550 frames Cell Events: 3862 mean_circ: 0.924168
Ran for 105050 frames Cell Events: 3881 mean_circ: 0.924165
Ran for 105550 frames Cell Events: 3903 mean_circ: 0.924161
Ran for 106050 frames Cell Events: 3922 mean_circ: 0.924275
Ran for 106550 frames Cell Events: 3940 mean_circ: 0.924241
Ran for 107050 frames Cell Events: 3967 mean_circ: 0.924148
Ran for 108050 frames Cell Events: 4004 mean_circ: 0.92422
Ran for 108550 frames Cell Events: 4026 mean_circ: 0.924065
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Ran for 109550 frames Cell Events: 4067 mean_circ: 0.923968
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Ran for 120050 frames Cell Events: 4490 mean_circ: 0.924219
Ran for 120550 frames Cell Events: 4513 mean_circ: 0.924223
Ran for 121050 frames Cell Events: 4532 mean_circ: 0.924125
Ran for 121550 frames Cell Events: 4558 mean_circ: 0.924001
Ran for 124050 frames Cell Events: 4662 mean_circ: 0.923905
Ran for 124550 frames Cell Events: 4673 mean_circ: 0.923958
Ran for 125050 frames Cell Events: 4689 mean_circ: 0.924029
Ran for 126050 frames Cell Events: 4734 mean_circ: 0.923991
Ran for 127050 frames Cell Events: 4765 mean_circ: 0.924058
Ran for 127550 frames Cell Events: 4791 mean_circ: 0.92412
Ran for 128550 frames Cell Events: 4835 mean_circ: 0.924008
Ran for 129050 frames Cell Events: 4855 mean_circ: 0.923989
Ran for 129550 frames Cell Events: 4870 mean_circ: 0.923943
Ran for 130050 frames Cell Events: 4891 mean_circ: 0.923898
Ran for 130550 frames Cell Events: 4911 mean_circ: 0.923895
Ran for 131050 frames Cell Events: 4928 mean_circ: 0.923857
Ran for 131550 frames Cell Events: 4957 mean_circ: 0.923905
Ran for 132050 frames Cell Events: 4979 mean_circ: 0.923896
Ran for 132550 frames Cell Events: 4999 mean_circ: 0.923901
Ran for 133050 frames Cell Events: 5029 mean_circ: 0.923931
Ran for 134050 frames Cell Events: 5068 mean_circ: 0.923894
Ran for 134550 frames Cell Events: 5093 mean_circ: 0.923925
Ran for 135050 frames Cell Events: 5104 mean_circ: 0.923883
Ran for 135550 frames Cell Events: 5130 mean_circ: 0.923876
Ran for 136050 frames Cell Events: 5148 mean_circ: 0.923902
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Ran for 137550 frames Cell Events: 5215 mean_circ: 0.923811
Ran for 138050 frames Cell Events: 5230 mean_circ: 0.923824
Ran for 138550 frames Cell Events: 5248 mean_circ: 0.923865
Ran for 139050 frames Cell Events: 5263 mean_circ: 0.92387
Ran for 140050 frames Cell Events: 5299 mean_circ: 0.923877
Ran for 140550 frames Cell Events: 5325 mean_circ: 0.923829
Ran for 141050 frames Cell Events: 5343 mean_circ: 0.92384
Ran for 141550 frames Cell Events: 5368 mean_circ: 0.92379
Ran for 142550 frames Cell Events: 5404 mean_circ: 0.923739
Ran for 143050 frames Cell Events: 5415 mean_circ: 0.923793
Ran for 143550 frames Cell Events: 5441 mean_circ: 0.923797
Ran for 144050 frames Cell Events: 5454 mean_circ: 0.923803
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Ran for 145550 frames Cell Events: 5503 mean_circ: 0.923896
Ran for 146050 frames Cell Events: 5529 mean_circ: 0.92389
Ran for 146550 frames Cell Events: 5543 mean_circ: 0.923921
Ran for 147050 frames Cell Events: 5561 mean_circ: 0.923885
Ran for 147550 frames Cell Events: 5571 mean_circ: 0.923898
Ran for 148050 frames Cell Events: 5587 mean_circ: 0.923883
Ran for 148550 frames Cell Events: 5601 mean_circ: 0.923914
Ran for 149050 frames Cell Events: 5638 mean_circ: 0.923966
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Ran for 151050 frames Cell Events: 5696 mean_circ: 0.923846
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Ran for 152550 frames Cell Events: 5763 mean_circ: 0.923799
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Ran for 157050 frames Cell Events: 5937 mean_circ: 0.92381
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Ran for 174050 frames Cell Events: 6598 mean_circ: 0.923335
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Ran for 176550 frames Cell Events: 6700 mean_circ: 0.923443
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Ran for 181550 frames Cell Events: 6900 mean_circ: 0.923647
Ran for 182050 frames Cell Events: 6912 mean_circ: 0.923676
Ran for 183550 frames Cell Events: 6979 mean_circ: 0.923591
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Ran for 187550 frames Cell Events: 7119 mean_circ: 0.923659
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Ran for 190050 frames Cell Events: 7218 mean_circ: 0.923651
Ran for 193050 frames Cell Events: 7354 mean_circ: 0.923776
Ran for 193550 frames Cell Events: 7368 mean_circ: 0.923776
Ran for 194050 frames Cell Events: 7399 mean_circ: 0.923746
Ran for 196550 frames Cell Events: 7534 mean_circ: 0.923701
Ran for 197050 frames Cell Events: 7564 mean_circ: 0.923724
Ran for 197550 frames Cell Events: 7579 mean_circ: 0.92372
Ran for 198050 frames Cell Events: 7598 mean_circ: 0.9237
Ran for 199050 frames Cell Events: 7615 mean_circ: 0.923653
Ran for 199550 frames Cell Events: 7625 mean_circ: 0.923675
Ran for 200050 frames Cell Events: 7646 mean_circ: 0.923676
Ran for 200550 frames Cell Events: 7672 mean_circ: 0.923686
Ran for 201050 frames Cell Events: 7697 mean_circ: 0.923602
Ran for 203050 frames Cell Events: 7791 mean_circ: 0.923373
Ran for 204550 frames Cell Events: 7850 mean_circ: 0.92327
Ran for 205050 frames Cell Events: 7876 mean_circ: 0.923272
Ran for 205550 frames Cell Events: 7901 mean_circ: 0.923277
Ran for 206050 frames Cell Events: 7920 mean_circ: 0.9233
Ran for 206550 frames Cell Events: 7943 mean_circ: 0.92332
Ran for 207550 frames Cell Events: 7982 mean_circ: 0.923334
Ran for 208050 frames Cell Events: 8014 mean_circ: 0.923356
End this measurment..
Stopping time: 5.58782e+008
****************************************
Measurement finished
Mean Circularitiy: 0.923354
Mean Area: 594.503
Mean FL-1 intensity: 2961.85
Mean FL-2 intensity: 4337.09
Mean FL-3 intensity: 0
frames processed: 204722
Total Time: 104286ms
Number of Runs (new bg img) 0
Frames collected: 208419
Skipped frames: 95835
Rescued Frames: 4550552
Samples DAQ: 52104680
Samples written: 104209252
Samples per Channel: 52104626
FPS: 1998.53
Samples per second: 499631
Expected samples/s: 500000
Events: 8037
Events per second: 77.0668
jumped back (PMT): 24
#Frames read, frequency
# 0 frames :290102
# 1 frames :111275
# 2 frames :4383
# 3 frames :1377
# 4 frames :647
# 5 frames :240
# 6 frames :2959
# 7 frames :1663
# 8 frames :1273
# 9 frames :660
# 10 frames :392
# 11 frames :288
# 12 frames :318
# 13 frames :188
# 14 frames :223
# 15 frames :160
# 16 frames :119
# 17 frames :77
# 18 frames :58
# 19 frames :47
# 20 frames :52
# 21 frames :40
# 22 frames :41
# 23 frames :23
# 24 frames :24
# 25 frames :12
# 26 frames :14
# 27 frames :14
# 28 frames :10
# 29 frames :9
# 30 frames :12
# 31 frames :5
# 32 frames :5
# 33 frames :3
# 34 frames :2
# 35 frames :4
# 36 frames :5
# 37 frames :1
# 38 frames :3
# 39 frames :3
# 40 frames :1
# 42 frames :1
# 47 frames :2
# 48 frames :1
# 49 frames :1
# 50 frames :1
# 51 frames :1
# 52 frames :2
# 53 frames :1
# 54 frames :1
# 71 frames :1
# 72 frames :1
# 76 frames :1
M1_para.ini
[General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100
[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0
#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 25.8807
parameters.txt
#fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0