Click on the log titles below to expand the log entries present in this DC resource.
dclab-export_2024-07-09_19.19.28
{"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc
{
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "18a301adcd477a16656af78a5fd5d606",
"name": "M1_20170522_1336_dsRNA_c8_faster.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-09",
"time": "17:19:49"
}
}
dclab-tdms2rtdc-warnings
LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8197 (max 8198) in '8197'.
FG_Config.mcf
[Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;
[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;
[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini
[Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6
[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini
[EVENT LOG] fRT-DC V1.06170206
Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 25.9198
Start pulse train for BG Aquisition..
Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 6.23639e+008
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 550 frames Cell Events: 21 mean_circ: 0.947954
Ran for 1050 frames Cell Events: 45 mean_circ: 0.946517
Ran for 2050 frames Cell Events: 84 mean_circ: 0.933227
Ran for 2550 frames Cell Events: 102 mean_circ: 0.93496
Ran for 3050 frames Cell Events: 125 mean_circ: 0.935729
Ran for 3550 frames Cell Events: 137 mean_circ: 0.936402
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Ran for 6050 frames Cell Events: 232 mean_circ: 0.935532
Ran for 6550 frames Cell Events: 241 mean_circ: 0.9346
Ran for 7050 frames Cell Events: 267 mean_circ: 0.934371
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Ran for 10050 frames Cell Events: 372 mean_circ: 0.937632
Ran for 10550 frames Cell Events: 392 mean_circ: 0.93747
Ran for 11550 frames Cell Events: 435 mean_circ: 0.937714
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Ran for 12550 frames Cell Events: 470 mean_circ: 0.938089
Ran for 13550 frames Cell Events: 516 mean_circ: 0.93728
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Ran for 35050 frames Cell Events: 1299 mean_circ: 0.936484
Ran for 35550 frames Cell Events: 1325 mean_circ: 0.936528
Ran for 36050 frames Cell Events: 1342 mean_circ: 0.936623
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Ran for 67050 frames Cell Events: 2472 mean_circ: 0.935121
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Ran for 74050 frames Cell Events: 2741 mean_circ: 0.935655
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Ran for 75050 frames Cell Events: 2770 mean_circ: 0.935624
Ran for 75550 frames Cell Events: 2788 mean_circ: 0.935619
Ran for 76050 frames Cell Events: 2805 mean_circ: 0.935662
Ran for 76550 frames Cell Events: 2819 mean_circ: 0.935611
Ran for 77050 frames Cell Events: 2842 mean_circ: 0.935605
Ran for 77550 frames Cell Events: 2865 mean_circ: 0.935521
Ran for 78050 frames Cell Events: 2888 mean_circ: 0.935568
Ran for 78550 frames Cell Events: 2903 mean_circ: 0.935533
Ran for 79050 frames Cell Events: 2924 mean_circ: 0.935553
Ran for 79550 frames Cell Events: 2939 mean_circ: 0.935512
Ran for 80550 frames Cell Events: 2971 mean_circ: 0.935547
Ran for 83050 frames Cell Events: 3066 mean_circ: 0.935549
Ran for 83550 frames Cell Events: 3078 mean_circ: 0.93559
Ran for 84550 frames Cell Events: 3091 mean_circ: 0.935506
Ran for 85050 frames Cell Events: 3098 mean_circ: 0.935518
Ran for 86050 frames Cell Events: 3132 mean_circ: 0.935575
Ran for 86550 frames Cell Events: 3144 mean_circ: 0.93554
Ran for 87050 frames Cell Events: 3168 mean_circ: 0.935567
Ran for 88050 frames Cell Events: 3205 mean_circ: 0.935395
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Ran for 91050 frames Cell Events: 3300 mean_circ: 0.935475
Ran for 92050 frames Cell Events: 3338 mean_circ: 0.935541
Ran for 93050 frames Cell Events: 3374 mean_circ: 0.935545
Ran for 93550 frames Cell Events: 3393 mean_circ: 0.935574
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Ran for 96550 frames Cell Events: 3492 mean_circ: 0.935497
Ran for 97050 frames Cell Events: 3509 mean_circ: 0.935522
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Ran for 100550 frames Cell Events: 3635 mean_circ: 0.935766
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Ran for 103050 frames Cell Events: 3722 mean_circ: 0.93585
Ran for 103550 frames Cell Events: 3732 mean_circ: 0.935829
Ran for 104050 frames Cell Events: 3748 mean_circ: 0.93581
Ran for 106050 frames Cell Events: 3823 mean_circ: 0.935741
Ran for 106550 frames Cell Events: 3842 mean_circ: 0.935639
Ran for 107050 frames Cell Events: 3861 mean_circ: 0.935613
Ran for 108050 frames Cell Events: 3905 mean_circ: 0.935543
Ran for 108550 frames Cell Events: 3915 mean_circ: 0.93556
Ran for 109050 frames Cell Events: 3940 mean_circ: 0.935539
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Ran for 110050 frames Cell Events: 3980 mean_circ: 0.935537
Ran for 110550 frames Cell Events: 3993 mean_circ: 0.935559
Ran for 111050 frames Cell Events: 4013 mean_circ: 0.93554
Ran for 112050 frames Cell Events: 4060 mean_circ: 0.935597
Ran for 112550 frames Cell Events: 4081 mean_circ: 0.935591
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Ran for 115550 frames Cell Events: 4192 mean_circ: 0.935493
Ran for 116050 frames Cell Events: 4222 mean_circ: 0.935523
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Ran for 121050 frames Cell Events: 4390 mean_circ: 0.935463
Ran for 121550 frames Cell Events: 4410 mean_circ: 0.935513
Ran for 122050 frames Cell Events: 4434 mean_circ: 0.935458
Ran for 123050 frames Cell Events: 4472 mean_circ: 0.935517
Ran for 124050 frames Cell Events: 4504 mean_circ: 0.935564
Ran for 125050 frames Cell Events: 4545 mean_circ: 0.935671
Ran for 125550 frames Cell Events: 4558 mean_circ: 0.935697
Ran for 126050 frames Cell Events: 4579 mean_circ: 0.935714
Ran for 126550 frames Cell Events: 4592 mean_circ: 0.935753
Ran for 127050 frames Cell Events: 4609 mean_circ: 0.935711
Ran for 128050 frames Cell Events: 4650 mean_circ: 0.935769
Ran for 128550 frames Cell Events: 4673 mean_circ: 0.935832
Ran for 129050 frames Cell Events: 4687 mean_circ: 0.935835
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Ran for 130050 frames Cell Events: 4726 mean_circ: 0.935774
Ran for 131050 frames Cell Events: 4757 mean_circ: 0.935777
Ran for 132550 frames Cell Events: 4819 mean_circ: 0.935754
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Ran for 142550 frames Cell Events: 5137 mean_circ: 0.935909
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Ran for 146550 frames Cell Events: 5267 mean_circ: 0.936051
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Ran for 157050 frames Cell Events: 5618 mean_circ: 0.935829
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Ran for 172550 frames Cell Events: 6181 mean_circ: 0.935986
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Ran for 176050 frames Cell Events: 6326 mean_circ: 0.936021
Ran for 176550 frames Cell Events: 6346 mean_circ: 0.936034
Ran for 177550 frames Cell Events: 6397 mean_circ: 0.936068
Ran for 178050 frames Cell Events: 6413 mean_circ: 0.936057
Ran for 178550 frames Cell Events: 6430 mean_circ: 0.93606
Ran for 179050 frames Cell Events: 6447 mean_circ: 0.936062
Ran for 180050 frames Cell Events: 6485 mean_circ: 0.936028
Ran for 180550 frames Cell Events: 6495 mean_circ: 0.936035
Ran for 181050 frames Cell Events: 6509 mean_circ: 0.936056
Ran for 181550 frames Cell Events: 6526 mean_circ: 0.936073
Ran for 182050 frames Cell Events: 6546 mean_circ: 0.936091
Ran for 182550 frames Cell Events: 6562 mean_circ: 0.936121
Ran for 183050 frames Cell Events: 6591 mean_circ: 0.936086
Ran for 184550 frames Cell Events: 6654 mean_circ: 0.936116
Ran for 185050 frames Cell Events: 6670 mean_circ: 0.936073
Ran for 185550 frames Cell Events: 6690 mean_circ: 0.936078
Ran for 186050 frames Cell Events: 6707 mean_circ: 0.936029
Ran for 186550 frames Cell Events: 6727 mean_circ: 0.936056
Ran for 187050 frames Cell Events: 6739 mean_circ: 0.936062
Ran for 187550 frames Cell Events: 6764 mean_circ: 0.936039
Ran for 188050 frames Cell Events: 6796 mean_circ: 0.936049
Ran for 188550 frames Cell Events: 6810 mean_circ: 0.935988
Ran for 189050 frames Cell Events: 6828 mean_circ: 0.936002
Ran for 189550 frames Cell Events: 6852 mean_circ: 0.935982
Ran for 190050 frames Cell Events: 6864 mean_circ: 0.935962
Ran for 190550 frames Cell Events: 6885 mean_circ: 0.935981
Ran for 191050 frames Cell Events: 6906 mean_circ: 0.935958
Ran for 192050 frames Cell Events: 6969 mean_circ: 0.935934
Ran for 192550 frames Cell Events: 6987 mean_circ: 0.935931
Ran for 193050 frames Cell Events: 7005 mean_circ: 0.935919
Ran for 194050 frames Cell Events: 7049 mean_circ: 0.935889
Ran for 194550 frames Cell Events: 7070 mean_circ: 0.935883
Ran for 195050 frames Cell Events: 7079 mean_circ: 0.935895
Ran for 195550 frames Cell Events: 7095 mean_circ: 0.935899
Ran for 196550 frames Cell Events: 7140 mean_circ: 0.935896
Ran for 197050 frames Cell Events: 7159 mean_circ: 0.935925
Ran for 198050 frames Cell Events: 7202 mean_circ: 0.935936
Ran for 198550 frames Cell Events: 7225 mean_circ: 0.935899
Ran for 199550 frames Cell Events: 7276 mean_circ: 0.93592
Ran for 200050 frames Cell Events: 7301 mean_circ: 0.935921
Ran for 200550 frames Cell Events: 7319 mean_circ: 0.935842
Ran for 201050 frames Cell Events: 7334 mean_circ: 0.935821
Ran for 202050 frames Cell Events: 7384 mean_circ: 0.935799
Ran for 203050 frames Cell Events: 7422 mean_circ: 0.935839
Ran for 203550 frames Cell Events: 7446 mean_circ: 0.93586
Ran for 205050 frames Cell Events: 7512 mean_circ: 0.935904
Ran for 205550 frames Cell Events: 7529 mean_circ: 0.935889
Ran for 207550 frames Cell Events: 7622 mean_circ: 0.935899
Ran for 208050 frames Cell Events: 7638 mean_circ: 0.935924
Ran for 208550 frames Cell Events: 7661 mean_circ: 0.935903
Ran for 209050 frames Cell Events: 7680 mean_circ: 0.935908
Ran for 210050 frames Cell Events: 7723 mean_circ: 0.935919
Ran for 210550 frames Cell Events: 7737 mean_circ: 0.935912
Ran for 211050 frames Cell Events: 7758 mean_circ: 0.935871
Ran for 211550 frames Cell Events: 7778 mean_circ: 0.935853
Ran for 212550 frames Cell Events: 7822 mean_circ: 0.935881
Ran for 213050 frames Cell Events: 7839 mean_circ: 0.935879
Ran for 213550 frames Cell Events: 7860 mean_circ: 0.935876
Ran for 214050 frames Cell Events: 7878 mean_circ: 0.935866
Ran for 215550 frames Cell Events: 7939 mean_circ: 0.93583
Ran for 216550 frames Cell Events: 7979 mean_circ: 0.935765
Ran for 218550 frames Cell Events: 8074 mean_circ: 0.935714
Ran for 219050 frames Cell Events: 8090 mean_circ: 0.93573
Ran for 219550 frames Cell Events: 8106 mean_circ: 0.935743
Ran for 220550 frames Cell Events: 8138 mean_circ: 0.935707
Ran for 221050 frames Cell Events: 8156 mean_circ: 0.935676
Ran for 221550 frames Cell Events: 8177 mean_circ: 0.935644
End this measurment..
Stopping time: 6.2375e+008
****************************************
Measurement finished
Mean Circularitiy: 0.93566
Mean Area: 595.735
Mean FL-1 intensity: 2878.88
Mean FL-2 intensity: 4625.53
Mean FL-3 intensity: 0
frames processed: 220837
Total Time: 111036ms
Number of Runs (new bg img) 0
Frames collected: 221924
Skipped frames: 11769
Rescued Frames: 566569
Samples DAQ: 55480978
Samples written: 110961826
Samples per Channel: 55480913
FPS: 1998.67
Samples per second: 499666
Expected samples/s: 500000
Events: 8198
Events per second: 73.8318
jumped back (PMT): 5
#Frames read, frequency
# 0 frames :302130
# 1 frames :125132
# 2 frames :5152
# 3 frames :1466
# 4 frames :657
# 5 frames :173
# 6 frames :3162
# 7 frames :1620
# 8 frames :1239
# 9 frames :483
# 10 frames :491
# 11 frames :910
# 12 frames :381
# 13 frames :144
# 14 frames :171
# 15 frames :133
# 16 frames :89
# 17 frames :92
# 18 frames :45
# 19 frames :19
# 20 frames :25
# 21 frames :22
# 22 frames :23
# 23 frames :12
# 24 frames :17
# 25 frames :7
# 26 frames :4
# 27 frames :3
# 28 frames :3
# 29 frames :3
# 30 frames :1
# 31 frames :1
# 35 frames :1
# 36 frames :2
# 40 frames :1
# 43 frames :1
# 44 frames :1
# 49 frames :1
# 66 frames :1
M1_para.ini
[General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100
[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0
#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 25.9198
parameters.txt
#fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0