Click on the log titles below to expand the log entries present in this DC resource.
dclab-export_2024-07-09_19.30.31
{"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc
{
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "8734e004056d0b2500aa373fc717d6ee",
"name": "M1_20170522_1545_dsRNA_C1_faster.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-09",
"time": "17:30:55"
}
}
dclab-tdms2rtdc-warnings
LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 9194 (max 9195) in '9194'.
FG_Config.mcf
[Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;
[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;
[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini
[Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6
[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini
[EVENT LOG] fRT-DC V1.06170206
Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 25.9821
Start pulse train for BG Aquisition..
Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 6.31377e+008
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 550 frames Cell Events: 12 mean_circ: 0.940605
Ran for 1050 frames Cell Events: 28 mean_circ: 0.944934
Ran for 1550 frames Cell Events: 45 mean_circ: 0.939157
Ran for 2550 frames Cell Events: 70 mean_circ: 0.935999
Ran for 3550 frames Cell Events: 103 mean_circ: 0.935847
Ran for 4050 frames Cell Events: 118 mean_circ: 0.936085
Ran for 4550 frames Cell Events: 139 mean_circ: 0.936509
Ran for 5050 frames Cell Events: 164 mean_circ: 0.937212
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Ran for 7550 frames Cell Events: 251 mean_circ: 0.935681
Ran for 8050 frames Cell Events: 263 mean_circ: 0.935002
Ran for 9050 frames Cell Events: 288 mean_circ: 0.933875
Ran for 9550 frames Cell Events: 304 mean_circ: 0.934459
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Ran for 11050 frames Cell Events: 353 mean_circ: 0.933029
Ran for 11550 frames Cell Events: 375 mean_circ: 0.932235
Ran for 12550 frames Cell Events: 435 mean_circ: 0.93168
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Ran for 13550 frames Cell Events: 459 mean_circ: 0.932113
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Ran for 64550 frames Cell Events: 2270 mean_circ: 0.930111
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Ran for 65550 frames Cell Events: 2318 mean_circ: 0.930211
Ran for 66050 frames Cell Events: 2334 mean_circ: 0.930335
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Ran for 67050 frames Cell Events: 2367 mean_circ: 0.930466
Ran for 67550 frames Cell Events: 2393 mean_circ: 0.930647
Ran for 68550 frames Cell Events: 2443 mean_circ: 0.930849
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Ran for 69550 frames Cell Events: 2484 mean_circ: 0.930679
Ran for 70050 frames Cell Events: 2504 mean_circ: 0.930656
Ran for 70550 frames Cell Events: 2526 mean_circ: 0.93063
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Ran for 71550 frames Cell Events: 2558 mean_circ: 0.930647
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Ran for 73050 frames Cell Events: 2618 mean_circ: 0.930507
Ran for 73550 frames Cell Events: 2638 mean_circ: 0.930534
Ran for 74050 frames Cell Events: 2661 mean_circ: 0.930502
Ran for 75050 frames Cell Events: 2700 mean_circ: 0.930621
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Ran for 76550 frames Cell Events: 2757 mean_circ: 0.930531
Ran for 77550 frames Cell Events: 2793 mean_circ: 0.930603
Ran for 78050 frames Cell Events: 2819 mean_circ: 0.930679
Ran for 78550 frames Cell Events: 2847 mean_circ: 0.930638
Ran for 79050 frames Cell Events: 2860 mean_circ: 0.93053
Ran for 79550 frames Cell Events: 2892 mean_circ: 0.930549
Ran for 80050 frames Cell Events: 2910 mean_circ: 0.930612
Ran for 80550 frames Cell Events: 2922 mean_circ: 0.930641
Ran for 81050 frames Cell Events: 2939 mean_circ: 0.930565
Ran for 81550 frames Cell Events: 2961 mean_circ: 0.930571
Ran for 82050 frames Cell Events: 2978 mean_circ: 0.93052
Ran for 82550 frames Cell Events: 2998 mean_circ: 0.930523
Ran for 83050 frames Cell Events: 3022 mean_circ: 0.930509
Ran for 83550 frames Cell Events: 3043 mean_circ: 0.93052
Ran for 84050 frames Cell Events: 3058 mean_circ: 0.930522
Ran for 84550 frames Cell Events: 3075 mean_circ: 0.930579
Ran for 85050 frames Cell Events: 3091 mean_circ: 0.930584
Ran for 86050 frames Cell Events: 3146 mean_circ: 0.930542
Ran for 86550 frames Cell Events: 3164 mean_circ: 0.930545
Ran for 87050 frames Cell Events: 3182 mean_circ: 0.930526
Ran for 87550 frames Cell Events: 3203 mean_circ: 0.930531
Ran for 88050 frames Cell Events: 3224 mean_circ: 0.930561
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Ran for 90550 frames Cell Events: 3304 mean_circ: 0.930459
Ran for 91550 frames Cell Events: 3346 mean_circ: 0.930351
Ran for 92050 frames Cell Events: 3365 mean_circ: 0.930291
Ran for 92550 frames Cell Events: 3390 mean_circ: 0.930247
Ran for 93550 frames Cell Events: 3429 mean_circ: 0.930279
Ran for 94050 frames Cell Events: 3445 mean_circ: 0.930258
Ran for 94550 frames Cell Events: 3465 mean_circ: 0.930249
Ran for 95050 frames Cell Events: 3480 mean_circ: 0.930209
Ran for 95550 frames Cell Events: 3504 mean_circ: 0.930229
Ran for 96550 frames Cell Events: 3544 mean_circ: 0.930275
Ran for 97050 frames Cell Events: 3564 mean_circ: 0.930276
Ran for 97550 frames Cell Events: 3591 mean_circ: 0.93025
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Ran for 100050 frames Cell Events: 3665 mean_circ: 0.930231
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Ran for 101550 frames Cell Events: 3718 mean_circ: 0.930262
Ran for 104050 frames Cell Events: 3864 mean_circ: 0.930297
Ran for 104550 frames Cell Events: 3881 mean_circ: 0.93028
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Ran for 105550 frames Cell Events: 3918 mean_circ: 0.930315
Ran for 106050 frames Cell Events: 3948 mean_circ: 0.930371
Ran for 106550 frames Cell Events: 3973 mean_circ: 0.930401
Ran for 108050 frames Cell Events: 4044 mean_circ: 0.930457
Ran for 108550 frames Cell Events: 4063 mean_circ: 0.93035
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Ran for 109550 frames Cell Events: 4101 mean_circ: 0.930361
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Ran for 110550 frames Cell Events: 4141 mean_circ: 0.93022
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Ran for 115050 frames Cell Events: 4308 mean_circ: 0.9304
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Ran for 118050 frames Cell Events: 4437 mean_circ: 0.930207
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Ran for 120050 frames Cell Events: 4513 mean_circ: 0.929977
Ran for 120550 frames Cell Events: 4525 mean_circ: 0.929971
Ran for 121050 frames Cell Events: 4541 mean_circ: 0.92994
Ran for 122050 frames Cell Events: 4577 mean_circ: 0.92987
Ran for 123050 frames Cell Events: 4612 mean_circ: 0.929793
Ran for 123550 frames Cell Events: 4629 mean_circ: 0.929787
Ran for 124050 frames Cell Events: 4666 mean_circ: 0.929776
Ran for 124550 frames Cell Events: 4681 mean_circ: 0.929824
Ran for 125050 frames Cell Events: 4706 mean_circ: 0.929861
Ran for 125550 frames Cell Events: 4727 mean_circ: 0.929837
Ran for 126550 frames Cell Events: 4763 mean_circ: 0.929796
Ran for 127550 frames Cell Events: 4797 mean_circ: 0.92978
Ran for 128050 frames Cell Events: 4824 mean_circ: 0.929724
Ran for 128550 frames Cell Events: 4844 mean_circ: 0.929717
Ran for 130050 frames Cell Events: 4892 mean_circ: 0.929656
Ran for 131050 frames Cell Events: 4937 mean_circ: 0.929588
Ran for 131550 frames Cell Events: 4954 mean_circ: 0.929618
Ran for 132050 frames Cell Events: 4970 mean_circ: 0.929644
Ran for 132550 frames Cell Events: 4992 mean_circ: 0.929708
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Ran for 168050 frames Cell Events: 6354 mean_circ: 0.929195
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Ran for 180550 frames Cell Events: 6808 mean_circ: 0.928994
Ran for 181050 frames Cell Events: 6831 mean_circ: 0.928962
Ran for 181550 frames Cell Events: 6843 mean_circ: 0.928919
Ran for 182050 frames Cell Events: 6854 mean_circ: 0.928944
Ran for 182550 frames Cell Events: 6870 mean_circ: 0.928961
Ran for 183550 frames Cell Events: 6914 mean_circ: 0.928959
Ran for 184050 frames Cell Events: 6932 mean_circ: 0.928984
Ran for 184550 frames Cell Events: 6954 mean_circ: 0.929013
Ran for 185050 frames Cell Events: 6973 mean_circ: 0.928995
Ran for 185550 frames Cell Events: 6994 mean_circ: 0.929031
Ran for 186050 frames Cell Events: 7023 mean_circ: 0.929025
Ran for 187050 frames Cell Events: 7064 mean_circ: 0.928984
Ran for 189050 frames Cell Events: 7121 mean_circ: 0.929029
Ran for 189550 frames Cell Events: 7139 mean_circ: 0.929047
Ran for 190050 frames Cell Events: 7163 mean_circ: 0.929067
Ran for 191050 frames Cell Events: 7207 mean_circ: 0.929064
Ran for 191550 frames Cell Events: 7228 mean_circ: 0.929013
Ran for 192050 frames Cell Events: 7258 mean_circ: 0.928951
Ran for 193050 frames Cell Events: 7288 mean_circ: 0.928982
Ran for 193550 frames Cell Events: 7322 mean_circ: 0.929096
Ran for 194050 frames Cell Events: 7336 mean_circ: 0.929127
Ran for 194550 frames Cell Events: 7365 mean_circ: 0.929133
Ran for 195050 frames Cell Events: 7389 mean_circ: 0.929092
Ran for 195550 frames Cell Events: 7416 mean_circ: 0.929056
Ran for 196550 frames Cell Events: 7468 mean_circ: 0.929051
Ran for 197550 frames Cell Events: 7523 mean_circ: 0.929062
Ran for 198050 frames Cell Events: 7548 mean_circ: 0.929059
Ran for 198550 frames Cell Events: 7566 mean_circ: 0.929053
Ran for 199050 frames Cell Events: 7588 mean_circ: 0.929057
Ran for 199550 frames Cell Events: 7608 mean_circ: 0.929054
Ran for 200050 frames Cell Events: 7632 mean_circ: 0.929053
Ran for 200550 frames Cell Events: 7656 mean_circ: 0.929014
Ran for 201050 frames Cell Events: 7667 mean_circ: 0.929047
Ran for 201550 frames Cell Events: 7693 mean_circ: 0.929047
Ran for 202050 frames Cell Events: 7719 mean_circ: 0.929079
Ran for 202550 frames Cell Events: 7739 mean_circ: 0.929072
Ran for 203050 frames Cell Events: 7756 mean_circ: 0.929042
Ran for 203550 frames Cell Events: 7786 mean_circ: 0.929058
Ran for 204550 frames Cell Events: 7833 mean_circ: 0.929025
Ran for 205050 frames Cell Events: 7862 mean_circ: 0.929035
Ran for 206050 frames Cell Events: 7886 mean_circ: 0.929008
Ran for 207050 frames Cell Events: 7919 mean_circ: 0.929074
Ran for 208550 frames Cell Events: 7988 mean_circ: 0.929075
Ran for 209050 frames Cell Events: 8017 mean_circ: 0.929071
Ran for 210050 frames Cell Events: 8065 mean_circ: 0.929056
Ran for 211550 frames Cell Events: 8125 mean_circ: 0.929072
Ran for 212050 frames Cell Events: 8139 mean_circ: 0.929096
Ran for 213050 frames Cell Events: 8186 mean_circ: 0.929103
Ran for 213550 frames Cell Events: 8205 mean_circ: 0.929105
Ran for 214050 frames Cell Events: 8226 mean_circ: 0.929124
Ran for 214550 frames Cell Events: 8247 mean_circ: 0.929128
Ran for 215050 frames Cell Events: 8274 mean_circ: 0.929123
Ran for 215550 frames Cell Events: 8287 mean_circ: 0.929105
Ran for 216050 frames Cell Events: 8308 mean_circ: 0.929108
Ran for 217050 frames Cell Events: 8359 mean_circ: 0.92909
Ran for 217550 frames Cell Events: 8384 mean_circ: 0.929107
Ran for 218050 frames Cell Events: 8407 mean_circ: 0.929125
Ran for 219050 frames Cell Events: 8418 mean_circ: 0.929145
Ran for 219550 frames Cell Events: 8432 mean_circ: 0.929121
Ran for 220050 frames Cell Events: 8448 mean_circ: 0.929136
Ran for 220550 frames Cell Events: 8472 mean_circ: 0.929182
Ran for 221550 frames Cell Events: 8531 mean_circ: 0.929158
Ran for 222050 frames Cell Events: 8551 mean_circ: 0.929192
Ran for 222550 frames Cell Events: 8557 mean_circ: 0.929194
Ran for 224050 frames Cell Events: 8615 mean_circ: 0.929171
Ran for 224550 frames Cell Events: 8628 mean_circ: 0.929187
Ran for 225050 frames Cell Events: 8652 mean_circ: 0.929207
Ran for 227050 frames Cell Events: 8748 mean_circ: 0.929186
Ran for 227550 frames Cell Events: 8762 mean_circ: 0.92918
Ran for 228050 frames Cell Events: 8773 mean_circ: 0.929178
Ran for 228550 frames Cell Events: 8800 mean_circ: 0.929155
Ran for 229050 frames Cell Events: 8823 mean_circ: 0.92912
Ran for 229550 frames Cell Events: 8842 mean_circ: 0.929076
Ran for 230050 frames Cell Events: 8863 mean_circ: 0.929067
Ran for 231050 frames Cell Events: 8876 mean_circ: 0.92906
Ran for 231550 frames Cell Events: 8888 mean_circ: 0.929053
Ran for 232050 frames Cell Events: 8901 mean_circ: 0.929051
Ran for 233550 frames Cell Events: 8946 mean_circ: 0.929029
Ran for 235050 frames Cell Events: 9020 mean_circ: 0.929017
Ran for 237550 frames Cell Events: 9152 mean_circ: 0.928988
End this measurment..
Stopping time: 6.31496e+008
****************************************
Measurement finished
Mean Circularitiy: 0.92895
Mean Area: 622.416
Mean FL-1 intensity: 3027.96
Mean FL-2 intensity: 4706.78
Mean FL-3 intensity: 0
frames processed: 234258
Total Time: 119318ms
Number of Runs (new bg img) 0
Frames collected: 238500
Skipped frames: 47973
Rescued Frames: 2416156
Samples DAQ: 59625041
Samples written: 119249786
Samples per Channel: 59624893
FPS: 1998.86
Samples per second: 499715
Expected samples/s: 500000
Events: 9195
Events per second: 77.0631
jumped back (PMT): 9
#Frames read, frequency
# 0 frames :323779
# 1 frames :135705
# 2 frames :5428
# 3 frames :1380
# 4 frames :497
# 5 frames :172
# 6 frames :4422
# 7 frames :1685
# 8 frames :1541
# 9 frames :505
# 10 frames :397
# 11 frames :201
# 12 frames :327
# 13 frames :154
# 14 frames :223
# 15 frames :155
# 16 frames :81
# 17 frames :71
# 18 frames :50
# 19 frames :18
# 20 frames :35
# 21 frames :30
# 22 frames :23
# 23 frames :17
# 24 frames :18
# 25 frames :7
# 26 frames :6
# 27 frames :11
# 28 frames :5
# 29 frames :5
# 30 frames :2
# 31 frames :2
# 32 frames :1
# 33 frames :2
# 34 frames :1
# 35 frames :3
# 36 frames :2
# 37 frames :1
# 40 frames :1
# 42 frames :1
# 44 frames :2
# 52 frames :1
# 70 frames :1
# 84 frames :1
M1_para.ini
[General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100
[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0
#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 25.9821
parameters.txt
#fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0