Click on the log titles below to expand the log entries present in this DC resource.
dclab-export_2024-07-09_19.38.03
{"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc
{
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "74f431f208feb75f5c20cba45a71948c",
"name": "M1_20170522_1718_dsRNA_E7_col.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-09",
"time": "17:38:24"
}
}
dclab-tdms2rtdc-warnings
LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8485 (max 8487) in '8485'.
FG_Config.mcf
[Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;
[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;
[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini
[Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6
[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini
[EVENT LOG] fRT-DC V1.06170206
Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 25.4909
Start pulse train for BG Aquisition..
Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 6.36927e+008
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 550 frames Cell Events: 18 mean_circ: 0.886652
Ran for 1050 frames Cell Events: 31 mean_circ: 0.910903
Ran for 1550 frames Cell Events: 47 mean_circ: 0.915956
Ran for 2050 frames Cell Events: 68 mean_circ: 0.929057
Ran for 3050 frames Cell Events: 109 mean_circ: 0.931497
Ran for 3550 frames Cell Events: 123 mean_circ: 0.932619
Ran for 4050 frames Cell Events: 144 mean_circ: 0.933608
Ran for 4550 frames Cell Events: 159 mean_circ: 0.935766
Ran for 5050 frames Cell Events: 173 mean_circ: 0.935885
Ran for 5550 frames Cell Events: 187 mean_circ: 0.936102
Ran for 6050 frames Cell Events: 205 mean_circ: 0.93713
Ran for 6550 frames Cell Events: 225 mean_circ: 0.936101
Ran for 7050 frames Cell Events: 236 mean_circ: 0.937488
Ran for 7550 frames Cell Events: 260 mean_circ: 0.937462
Ran for 8050 frames Cell Events: 271 mean_circ: 0.937306
Ran for 8550 frames Cell Events: 286 mean_circ: 0.937519
Ran for 9550 frames Cell Events: 335 mean_circ: 0.937707
Ran for 10550 frames Cell Events: 375 mean_circ: 0.939557
Ran for 11050 frames Cell Events: 391 mean_circ: 0.939609
Ran for 11550 frames Cell Events: 411 mean_circ: 0.939774
Ran for 12050 frames Cell Events: 426 mean_circ: 0.939197
Ran for 13050 frames Cell Events: 463 mean_circ: 0.939724
Ran for 14050 frames Cell Events: 499 mean_circ: 0.940374
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Ran for 15050 frames Cell Events: 536 mean_circ: 0.940167
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Ran for 32550 frames Cell Events: 1181 mean_circ: 0.94181
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Ran for 35050 frames Cell Events: 1262 mean_circ: 0.941348
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Ran for 36050 frames Cell Events: 1316 mean_circ: 0.941667
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Ran for 37050 frames Cell Events: 1352 mean_circ: 0.941764
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Ran for 63550 frames Cell Events: 2461 mean_circ: 0.942108
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Ran for 64550 frames Cell Events: 2500 mean_circ: 0.94217
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Ran for 67050 frames Cell Events: 2585 mean_circ: 0.942263
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Ran for 68050 frames Cell Events: 2613 mean_circ: 0.942333
Ran for 68550 frames Cell Events: 2635 mean_circ: 0.942338
Ran for 69050 frames Cell Events: 2656 mean_circ: 0.942403
Ran for 70050 frames Cell Events: 2695 mean_circ: 0.942543
Ran for 70550 frames Cell Events: 2711 mean_circ: 0.942563
Ran for 71550 frames Cell Events: 2736 mean_circ: 0.942587
Ran for 72050 frames Cell Events: 2765 mean_circ: 0.942595
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Ran for 74050 frames Cell Events: 2847 mean_circ: 0.942364
Ran for 75050 frames Cell Events: 2901 mean_circ: 0.9425
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Ran for 76550 frames Cell Events: 2960 mean_circ: 0.942516
Ran for 77550 frames Cell Events: 2999 mean_circ: 0.942628
Ran for 78050 frames Cell Events: 3015 mean_circ: 0.942604
Ran for 78550 frames Cell Events: 3032 mean_circ: 0.942679
Ran for 79550 frames Cell Events: 3074 mean_circ: 0.942761
Ran for 80050 frames Cell Events: 3096 mean_circ: 0.942782
Ran for 80550 frames Cell Events: 3122 mean_circ: 0.942813
Ran for 81050 frames Cell Events: 3145 mean_circ: 0.942927
Ran for 81550 frames Cell Events: 3160 mean_circ: 0.942962
Ran for 82050 frames Cell Events: 3176 mean_circ: 0.942975
Ran for 82550 frames Cell Events: 3189 mean_circ: 0.942965
Ran for 84050 frames Cell Events: 3241 mean_circ: 0.943013
Ran for 85050 frames Cell Events: 3280 mean_circ: 0.943054
Ran for 85550 frames Cell Events: 3297 mean_circ: 0.943116
Ran for 86050 frames Cell Events: 3311 mean_circ: 0.943137
Ran for 86550 frames Cell Events: 3327 mean_circ: 0.943109
Ran for 87050 frames Cell Events: 3341 mean_circ: 0.94317
Ran for 88050 frames Cell Events: 3383 mean_circ: 0.943076
Ran for 88550 frames Cell Events: 3408 mean_circ: 0.943134
Ran for 89050 frames Cell Events: 3419 mean_circ: 0.943148
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Ran for 90050 frames Cell Events: 3445 mean_circ: 0.943115
Ran for 91050 frames Cell Events: 3502 mean_circ: 0.942967
Ran for 91550 frames Cell Events: 3534 mean_circ: 0.942957
Ran for 92050 frames Cell Events: 3558 mean_circ: 0.942957
Ran for 93050 frames Cell Events: 3602 mean_circ: 0.943034
Ran for 94050 frames Cell Events: 3643 mean_circ: 0.942973
Ran for 94550 frames Cell Events: 3673 mean_circ: 0.942868
Ran for 95050 frames Cell Events: 3685 mean_circ: 0.942769
Ran for 95550 frames Cell Events: 3708 mean_circ: 0.942732
Ran for 96050 frames Cell Events: 3718 mean_circ: 0.942759
Ran for 96550 frames Cell Events: 3728 mean_circ: 0.942739
Ran for 97050 frames Cell Events: 3741 mean_circ: 0.942789
Ran for 98050 frames Cell Events: 3799 mean_circ: 0.942767
Ran for 99050 frames Cell Events: 3841 mean_circ: 0.942888
Ran for 100050 frames Cell Events: 3877 mean_circ: 0.942965
Ran for 100550 frames Cell Events: 3897 mean_circ: 0.94291
Ran for 101550 frames Cell Events: 3931 mean_circ: 0.943002
Ran for 102050 frames Cell Events: 3946 mean_circ: 0.94301
Ran for 102550 frames Cell Events: 3967 mean_circ: 0.942941
Ran for 103050 frames Cell Events: 3993 mean_circ: 0.942963
Ran for 103550 frames Cell Events: 4004 mean_circ: 0.942899
Ran for 104050 frames Cell Events: 4020 mean_circ: 0.942953
Ran for 104550 frames Cell Events: 4041 mean_circ: 0.94292
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Ran for 106050 frames Cell Events: 4112 mean_circ: 0.94298
Ran for 107050 frames Cell Events: 4165 mean_circ: 0.943058
Ran for 107550 frames Cell Events: 4181 mean_circ: 0.943085
Ran for 108550 frames Cell Events: 4235 mean_circ: 0.943056
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Ran for 110050 frames Cell Events: 4301 mean_circ: 0.943
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Ran for 111050 frames Cell Events: 4339 mean_circ: 0.942982
Ran for 112050 frames Cell Events: 4366 mean_circ: 0.942932
Ran for 113550 frames Cell Events: 4448 mean_circ: 0.94298
Ran for 114050 frames Cell Events: 4487 mean_circ: 0.943084
Ran for 114550 frames Cell Events: 4513 mean_circ: 0.943073
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Ran for 116050 frames Cell Events: 4580 mean_circ: 0.943031
Ran for 117050 frames Cell Events: 4630 mean_circ: 0.943084
Ran for 117550 frames Cell Events: 4643 mean_circ: 0.943124
Ran for 118550 frames Cell Events: 4682 mean_circ: 0.943175
Ran for 120050 frames Cell Events: 4759 mean_circ: 0.943188
Ran for 120550 frames Cell Events: 4780 mean_circ: 0.943168
Ran for 121050 frames Cell Events: 4802 mean_circ: 0.94313
Ran for 121550 frames Cell Events: 4821 mean_circ: 0.943084
Ran for 122050 frames Cell Events: 4839 mean_circ: 0.943059
Ran for 123550 frames Cell Events: 4911 mean_circ: 0.943018
Ran for 124050 frames Cell Events: 4915 mean_circ: 0.943013
Ran for 125050 frames Cell Events: 4940 mean_circ: 0.943072
Ran for 126050 frames Cell Events: 4984 mean_circ: 0.943058
Ran for 127550 frames Cell Events: 5050 mean_circ: 0.943125
Ran for 128050 frames Cell Events: 5068 mean_circ: 0.943119
Ran for 128550 frames Cell Events: 5084 mean_circ: 0.943143
Ran for 129050 frames Cell Events: 5107 mean_circ: 0.943092
Ran for 130050 frames Cell Events: 5144 mean_circ: 0.943079
Ran for 130550 frames Cell Events: 5164 mean_circ: 0.943097
Ran for 131050 frames Cell Events: 5186 mean_circ: 0.943107
Ran for 131550 frames Cell Events: 5214 mean_circ: 0.943185
Ran for 132050 frames Cell Events: 5234 mean_circ: 0.943158
Ran for 133550 frames Cell Events: 5302 mean_circ: 0.943118
Ran for 134050 frames Cell Events: 5324 mean_circ: 0.943148
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Ran for 139050 frames Cell Events: 5549 mean_circ: 0.943256
Ran for 140050 frames Cell Events: 5594 mean_circ: 0.943298
Ran for 141050 frames Cell Events: 5633 mean_circ: 0.943314
Ran for 141550 frames Cell Events: 5656 mean_circ: 0.943295
Ran for 143550 frames Cell Events: 5723 mean_circ: 0.943395
Ran for 144550 frames Cell Events: 5763 mean_circ: 0.943344
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Ran for 146050 frames Cell Events: 5820 mean_circ: 0.943377
Ran for 146550 frames Cell Events: 5852 mean_circ: 0.943392
Ran for 147050 frames Cell Events: 5882 mean_circ: 0.943408
Ran for 147550 frames Cell Events: 5896 mean_circ: 0.943434
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Ran for 151050 frames Cell Events: 6076 mean_circ: 0.943389
Ran for 151550 frames Cell Events: 6100 mean_circ: 0.943401
Ran for 152050 frames Cell Events: 6124 mean_circ: 0.943468
Ran for 152550 frames Cell Events: 6142 mean_circ: 0.943413
Ran for 153050 frames Cell Events: 6156 mean_circ: 0.943428
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Ran for 164050 frames Cell Events: 6630 mean_circ: 0.943517
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Ran for 167050 frames Cell Events: 6739 mean_circ: 0.94357
Ran for 168050 frames Cell Events: 6789 mean_circ: 0.943519
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Ran for 171050 frames Cell Events: 6926 mean_circ: 0.943576
Ran for 171550 frames Cell Events: 6944 mean_circ: 0.943607
Ran for 172050 frames Cell Events: 6967 mean_circ: 0.943598
Ran for 172550 frames Cell Events: 7000 mean_circ: 0.943578
Ran for 173050 frames Cell Events: 7018 mean_circ: 0.943586
Ran for 174050 frames Cell Events: 7055 mean_circ: 0.943606
Ran for 174550 frames Cell Events: 7070 mean_circ: 0.943598
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Ran for 180550 frames Cell Events: 7341 mean_circ: 0.943628
Ran for 181050 frames Cell Events: 7361 mean_circ: 0.943614
Ran for 181550 frames Cell Events: 7384 mean_circ: 0.943635
Ran for 182550 frames Cell Events: 7414 mean_circ: 0.943655
Ran for 183050 frames Cell Events: 7429 mean_circ: 0.943616
Ran for 185050 frames Cell Events: 7508 mean_circ: 0.943626
Ran for 185550 frames Cell Events: 7534 mean_circ: 0.94364
Ran for 186550 frames Cell Events: 7572 mean_circ: 0.943651
Ran for 187550 frames Cell Events: 7620 mean_circ: 0.94364
Ran for 188050 frames Cell Events: 7638 mean_circ: 0.943644
Ran for 188550 frames Cell Events: 7664 mean_circ: 0.943629
Ran for 190050 frames Cell Events: 7742 mean_circ: 0.94366
Ran for 190550 frames Cell Events: 7763 mean_circ: 0.943676
Ran for 191050 frames Cell Events: 7785 mean_circ: 0.943664
Ran for 191550 frames Cell Events: 7806 mean_circ: 0.943658
Ran for 193050 frames Cell Events: 7888 mean_circ: 0.943736
Ran for 193550 frames Cell Events: 7916 mean_circ: 0.943736
Ran for 194050 frames Cell Events: 7944 mean_circ: 0.943714
Ran for 195050 frames Cell Events: 7995 mean_circ: 0.943749
Ran for 195550 frames Cell Events: 8020 mean_circ: 0.94377
Ran for 196050 frames Cell Events: 8036 mean_circ: 0.943792
Ran for 197050 frames Cell Events: 8074 mean_circ: 0.943767
Ran for 198550 frames Cell Events: 8144 mean_circ: 0.943729
Ran for 199050 frames Cell Events: 8163 mean_circ: 0.943683
Ran for 199550 frames Cell Events: 8183 mean_circ: 0.943692
Ran for 200550 frames Cell Events: 8217 mean_circ: 0.943671
Ran for 201050 frames Cell Events: 8232 mean_circ: 0.943676
Ran for 202050 frames Cell Events: 8267 mean_circ: 0.943647
Ran for 202550 frames Cell Events: 8292 mean_circ: 0.943615
Ran for 204050 frames Cell Events: 8338 mean_circ: 0.943613
Ran for 204550 frames Cell Events: 8351 mean_circ: 0.943622
Ran for 206050 frames Cell Events: 8408 mean_circ: 0.943616
Ran for 207050 frames Cell Events: 8455 mean_circ: 0.943611
Ran for 207550 frames Cell Events: 8482 mean_circ: 0.943606
End this measurment..
Stopping time: 6.37031e+008
****************************************
Measurement finished
Mean Circularitiy: 0.943604
Mean Area: 658.816
Mean FL-1 intensity: 4953.04
Mean FL-2 intensity: 6065.42
Mean FL-3 intensity: 0
frames processed: 206107
Total Time: 103962ms
Number of Runs (new bg img) 0
Frames collected: 207768
Skipped frames: 76539
Rescued Frames: 3668704
Samples DAQ: 51942076
Samples written: 103884102
Samples per Channel: 51942051
FPS: 1998.51
Samples per second: 499628
Expected samples/s: 500000
Events: 8487
Events per second: 81.636
jumped back (PMT): 5206
#Frames read, frequency
# 0 frames :287548
# 1 frames :112245
# 2 frames :4960
# 3 frames :1177
# 4 frames :563
# 5 frames :192
# 6 frames :2635
# 7 frames :2225
# 8 frames :1332
# 9 frames :466
# 10 frames :418
# 11 frames :260
# 12 frames :296
# 13 frames :192
# 14 frames :238
# 15 frames :194
# 16 frames :128
# 17 frames :78
# 18 frames :53
# 19 frames :28
# 20 frames :35
# 21 frames :61
# 22 frames :31
# 23 frames :24
# 24 frames :27
# 25 frames :18
# 26 frames :18
# 27 frames :11
# 28 frames :10
# 29 frames :4
# 30 frames :8
# 31 frames :1
# 32 frames :4
# 33 frames :2
# 34 frames :2
# 35 frames :5
# 36 frames :2
# 37 frames :5
# 38 frames :2
# 39 frames :2
# 41 frames :1
# 43 frames :1
# 47 frames :1
# 50 frames :1
# 65 frames :1
M1_para.ini
[General]
Measurement Number = 1
Flow Rate [ul/s] = 0.04
Region = "Channel"
Channel width = 20
Current LED = 100
[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0
#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 25.4909
parameters.txt
#fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 300
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0