Click on the log titles below to expand the log entries present in this DC resource.
dclab-export_2024-07-09_19.42.02
{"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc
{
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "e15f51306a3024030ec566b8184b22fd",
"name": "M1_20170522_1759_dsRNA_E1_col.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-09",
"time": "17:42:28"
}
}
dclab-tdms2rtdc-warnings
LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 10001 (max 10002) in
'10001'.
FG_Config.mcf
[Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;
[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;
[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini
[Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6
[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini
[EVENT LOG] fRT-DC V1.06170206
Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 25.3807
Start pulse train for BG Aquisition..
Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 6.39434e+008
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 550 frames Cell Events: 15 mean_circ: 0.953234
Ran for 1050 frames Cell Events: 38 mean_circ: 0.941028
Ran for 2050 frames Cell Events: 66 mean_circ: 0.947287
Ran for 2550 frames Cell Events: 80 mean_circ: 0.946777
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Ran for 83550 frames Cell Events: 3151 mean_circ: 0.944717
Ran for 84050 frames Cell Events: 3170 mean_circ: 0.944727
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Ran for 92050 frames Cell Events: 3480 mean_circ: 0.944709
Ran for 93050 frames Cell Events: 3534 mean_circ: 0.944561
Ran for 94050 frames Cell Events: 3574 mean_circ: 0.944546
Ran for 95550 frames Cell Events: 3643 mean_circ: 0.944607
Ran for 97050 frames Cell Events: 3700 mean_circ: 0.944673
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Ran for 100050 frames Cell Events: 3819 mean_circ: 0.944816
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Ran for 101550 frames Cell Events: 3890 mean_circ: 0.944737
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Ran for 103550 frames Cell Events: 3975 mean_circ: 0.944854
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Ran for 120050 frames Cell Events: 4564 mean_circ: 0.94521
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Ran for 122050 frames Cell Events: 4648 mean_circ: 0.945274
Ran for 124050 frames Cell Events: 4708 mean_circ: 0.94525
Ran for 125050 frames Cell Events: 4741 mean_circ: 0.945194
Ran for 125550 frames Cell Events: 4776 mean_circ: 0.945194
Ran for 126550 frames Cell Events: 4810 mean_circ: 0.945147
Ran for 128050 frames Cell Events: 4881 mean_circ: 0.945183
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Ran for 130550 frames Cell Events: 5000 mean_circ: 0.945288
Ran for 131050 frames Cell Events: 5026 mean_circ: 0.945279
Ran for 131550 frames Cell Events: 5044 mean_circ: 0.945129
Ran for 132050 frames Cell Events: 5072 mean_circ: 0.945133
Ran for 132550 frames Cell Events: 5099 mean_circ: 0.945114
Ran for 133050 frames Cell Events: 5126 mean_circ: 0.945079
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Ran for 173550 frames Cell Events: 6964 mean_circ: 0.945196
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Ran for 194550 frames Cell Events: 7961 mean_circ: 0.944997
Ran for 195050 frames Cell Events: 7978 mean_circ: 0.944997
Ran for 195550 frames Cell Events: 8006 mean_circ: 0.945017
Ran for 196050 frames Cell Events: 8031 mean_circ: 0.945052
Ran for 196550 frames Cell Events: 8067 mean_circ: 0.945052
Ran for 198050 frames Cell Events: 8148 mean_circ: 0.945048
Ran for 199550 frames Cell Events: 8235 mean_circ: 0.945079
Ran for 200050 frames Cell Events: 8258 mean_circ: 0.945063
Ran for 200550 frames Cell Events: 8281 mean_circ: 0.945069
Ran for 201050 frames Cell Events: 8307 mean_circ: 0.945093
Ran for 201550 frames Cell Events: 8323 mean_circ: 0.945115
Ran for 203050 frames Cell Events: 8384 mean_circ: 0.945123
Ran for 203550 frames Cell Events: 8403 mean_circ: 0.945143
Ran for 205050 frames Cell Events: 8464 mean_circ: 0.945209
Ran for 205550 frames Cell Events: 8479 mean_circ: 0.945217
Ran for 207050 frames Cell Events: 8546 mean_circ: 0.94524
Ran for 208050 frames Cell Events: 8596 mean_circ: 0.945178
Ran for 208550 frames Cell Events: 8617 mean_circ: 0.945223
Ran for 209550 frames Cell Events: 8652 mean_circ: 0.945204
Ran for 210050 frames Cell Events: 8672 mean_circ: 0.945195
Ran for 211550 frames Cell Events: 8755 mean_circ: 0.945204
Ran for 212050 frames Cell Events: 8771 mean_circ: 0.945215
Ran for 212550 frames Cell Events: 8790 mean_circ: 0.945224
Ran for 213050 frames Cell Events: 8815 mean_circ: 0.945219
Ran for 214550 frames Cell Events: 8880 mean_circ: 0.945209
Ran for 215050 frames Cell Events: 8912 mean_circ: 0.945139
Ran for 215550 frames Cell Events: 8935 mean_circ: 0.945137
Ran for 216050 frames Cell Events: 8959 mean_circ: 0.945101
Ran for 216550 frames Cell Events: 8976 mean_circ: 0.94509
Ran for 217550 frames Cell Events: 9009 mean_circ: 0.945103
Ran for 219550 frames Cell Events: 9100 mean_circ: 0.945046
Ran for 220550 frames Cell Events: 9137 mean_circ: 0.945054
Ran for 222050 frames Cell Events: 9195 mean_circ: 0.945074
Ran for 222550 frames Cell Events: 9210 mean_circ: 0.945046
Ran for 223050 frames Cell Events: 9231 mean_circ: 0.94508
Ran for 224050 frames Cell Events: 9277 mean_circ: 0.945076
Ran for 224550 frames Cell Events: 9292 mean_circ: 0.945096
Ran for 225050 frames Cell Events: 9306 mean_circ: 0.945074
Ran for 225550 frames Cell Events: 9331 mean_circ: 0.945072
Ran for 226550 frames Cell Events: 9402 mean_circ: 0.945077
Ran for 227550 frames Cell Events: 9446 mean_circ: 0.945067
Ran for 228550 frames Cell Events: 9473 mean_circ: 0.945063
Ran for 229050 frames Cell Events: 9494 mean_circ: 0.945075
Ran for 230050 frames Cell Events: 9545 mean_circ: 0.945068
Ran for 231050 frames Cell Events: 9588 mean_circ: 0.945095
Ran for 231550 frames Cell Events: 9608 mean_circ: 0.945126
Ran for 232050 frames Cell Events: 9630 mean_circ: 0.945135
Ran for 232550 frames Cell Events: 9646 mean_circ: 0.945145
Ran for 233050 frames Cell Events: 9663 mean_circ: 0.945168
Ran for 233550 frames Cell Events: 9680 mean_circ: 0.945141
Ran for 234050 frames Cell Events: 9701 mean_circ: 0.945146
Ran for 234550 frames Cell Events: 9725 mean_circ: 0.945141
Ran for 235050 frames Cell Events: 9742 mean_circ: 0.94513
Ran for 238550 frames Cell Events: 9934 mean_circ: 0.945083
Ran for 239050 frames Cell Events: 9963 mean_circ: 0.945095
Ran for 239550 frames Cell Events: 9986 mean_circ: 0.945119
End this measurment.
because stop condition (time/nr of cells) was met
Stopping time: 6.39554e+008
****************************************
Measurement finished
Mean Circularitiy: 0.945131
Mean Area: 623.947
Mean FL-1 intensity: 3097.91
Mean FL-2 intensity: 4168.98
Mean FL-3 intensity: 0
frames processed: 239617
Total Time: 120019ms
Number of Runs (new bg img) 0
Frames collected: 239888
Skipped frames: 89460
Rescued Frames: 4314302
Samples DAQ: 59976204
Samples written: 119943964
Samples per Channel: 59971982
FPS: 1998.75
Samples per second: 499687
Expected samples/s: 500000
Events: 10002
Events per second: 83.3368
jumped back (PMT): 6083
#Frames read, frequency
# 0 frames :339715
# 1 frames :121134
# 2 frames :5474
# 3 frames :1830
# 4 frames :834
# 5 frames :317
# 6 frames :2270
# 7 frames :2210
# 8 frames :1243
# 9 frames :684
# 10 frames :1623
# 11 frames :498
# 12 frames :419
# 13 frames :207
# 14 frames :182
# 15 frames :224
# 16 frames :211
# 17 frames :125
# 18 frames :102
# 19 frames :62
# 20 frames :45
# 21 frames :62
# 22 frames :44
# 23 frames :36
# 24 frames :23
# 25 frames :27
# 26 frames :19
# 27 frames :9
# 28 frames :12
# 29 frames :7
# 30 frames :2
# 31 frames :3
# 32 frames :7
# 33 frames :6
# 34 frames :3
# 35 frames :4
# 36 frames :4
# 37 frames :3
# 38 frames :2
# 39 frames :2
# 41 frames :2
# 42 frames :2
# 46 frames :1
# 47 frames :1
# 48 frames :1
# 50 frames :1
# 51 frames :1
# 52 frames :1
# 53 frames :1
# 55 frames :1
# 60 frames :1
M1_para.ini
[General]
Measurement Number = 1
Flow Rate [ul/s] = 0.04
Region = "Channel"
Channel width = 20
Current LED = 100
[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0
#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 25.3807
parameters.txt
#fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 300
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0