Click on the log titles below to expand the log entries present in this DC resource.
dclab-export_2024-07-09_19.43.35
{"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc
{
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "8308dbef6b57a218defc76219b2b99d2",
"name": "M1_20170522_1815_dsRNA_E5_faster.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-09",
"time": "17:43:59"
}
}
dclab-tdms2rtdc-warnings
LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 9620 (max 9621) in '9620'.
FG_Config.mcf
[Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;
[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;
[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini
[Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6
[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini
[EVENT LOG] fRT-DC V1.06170206
Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 25.3934
Start pulse train for BG Aquisition..
Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 6.40344e+008
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 50 frames Cell Events: 3 mean_circ: 0.9387
Ran for 1050 frames Cell Events: 46 mean_circ: 0.934993
Ran for 1550 frames Cell Events: 79 mean_circ: 0.928865
Ran for 2050 frames Cell Events: 108 mean_circ: 0.926687
Ran for 2550 frames Cell Events: 142 mean_circ: 0.927465
Ran for 3050 frames Cell Events: 170 mean_circ: 0.928305
Ran for 4050 frames Cell Events: 196 mean_circ: 0.928054
Ran for 5550 frames Cell Events: 241 mean_circ: 0.926538
Ran for 6050 frames Cell Events: 280 mean_circ: 0.926904
Ran for 8550 frames Cell Events: 446 mean_circ: 0.92675
Ran for 9050 frames Cell Events: 470 mean_circ: 0.926275
Ran for 9550 frames Cell Events: 489 mean_circ: 0.926813
Ran for 10050 frames Cell Events: 513 mean_circ: 0.926991
Ran for 11050 frames Cell Events: 559 mean_circ: 0.925797
Ran for 11550 frames Cell Events: 580 mean_circ: 0.925967
Ran for 14550 frames Cell Events: 759 mean_circ: 0.925819
Ran for 15050 frames Cell Events: 788 mean_circ: 0.925819
Ran for 16050 frames Cell Events: 842 mean_circ: 0.926413
Ran for 16550 frames Cell Events: 870 mean_circ: 0.926109
Ran for 17050 frames Cell Events: 892 mean_circ: 0.926517
Ran for 18050 frames Cell Events: 964 mean_circ: 0.926186
Ran for 19550 frames Cell Events: 1073 mean_circ: 0.925981
Ran for 21550 frames Cell Events: 1190 mean_circ: 0.926178
Ran for 22550 frames Cell Events: 1236 mean_circ: 0.926233
Ran for 23550 frames Cell Events: 1304 mean_circ: 0.926074
Ran for 24050 frames Cell Events: 1324 mean_circ: 0.926372
Ran for 25550 frames Cell Events: 1405 mean_circ: 0.926413
Ran for 26050 frames Cell Events: 1434 mean_circ: 0.92635
Ran for 26550 frames Cell Events: 1472 mean_circ: 0.926242
Ran for 28050 frames Cell Events: 1559 mean_circ: 0.925739
Ran for 28550 frames Cell Events: 1597 mean_circ: 0.925895
Ran for 29050 frames Cell Events: 1644 mean_circ: 0.926035
Ran for 29550 frames Cell Events: 1660 mean_circ: 0.925861
Ran for 30550 frames Cell Events: 1710 mean_circ: 0.925687
Ran for 31050 frames Cell Events: 1741 mean_circ: 0.925498
Ran for 31550 frames Cell Events: 1767 mean_circ: 0.925377
Ran for 33550 frames Cell Events: 1881 mean_circ: 0.925434
Ran for 34050 frames Cell Events: 1903 mean_circ: 0.925472
Ran for 34550 frames Cell Events: 1933 mean_circ: 0.925319
Ran for 35050 frames Cell Events: 1954 mean_circ: 0.925429
Ran for 35550 frames Cell Events: 1981 mean_circ: 0.925412
Ran for 36050 frames Cell Events: 2005 mean_circ: 0.925458
Ran for 36550 frames Cell Events: 2032 mean_circ: 0.925468
Ran for 37050 frames Cell Events: 2052 mean_circ: 0.925391
Ran for 38050 frames Cell Events: 2082 mean_circ: 0.925531
Ran for 38550 frames Cell Events: 2114 mean_circ: 0.925498
Ran for 41050 frames Cell Events: 2264 mean_circ: 0.925986
Ran for 41550 frames Cell Events: 2293 mean_circ: 0.926007
Ran for 42050 frames Cell Events: 2315 mean_circ: 0.925964
Ran for 42550 frames Cell Events: 2345 mean_circ: 0.925855
Ran for 43050 frames Cell Events: 2365 mean_circ: 0.925886
Ran for 43550 frames Cell Events: 2393 mean_circ: 0.925755
Ran for 44050 frames Cell Events: 2412 mean_circ: 0.925595
Ran for 44550 frames Cell Events: 2438 mean_circ: 0.925714
Ran for 45050 frames Cell Events: 2470 mean_circ: 0.925762
Ran for 46050 frames Cell Events: 2538 mean_circ: 0.925961
Ran for 46550 frames Cell Events: 2573 mean_circ: 0.926124
Ran for 47050 frames Cell Events: 2606 mean_circ: 0.926352
Ran for 47550 frames Cell Events: 2642 mean_circ: 0.926277
Ran for 48550 frames Cell Events: 2691 mean_circ: 0.926114
Ran for 49050 frames Cell Events: 2708 mean_circ: 0.926196
Ran for 50050 frames Cell Events: 2756 mean_circ: 0.925917
Ran for 50550 frames Cell Events: 2793 mean_circ: 0.925919
Ran for 51050 frames Cell Events: 2816 mean_circ: 0.925856
Ran for 51550 frames Cell Events: 2859 mean_circ: 0.925868
Ran for 53050 frames Cell Events: 2959 mean_circ: 0.925651
Ran for 53550 frames Cell Events: 2981 mean_circ: 0.925668
Ran for 54050 frames Cell Events: 3011 mean_circ: 0.925661
Ran for 54550 frames Cell Events: 3029 mean_circ: 0.925844
Ran for 55550 frames Cell Events: 3090 mean_circ: 0.925668
Ran for 56550 frames Cell Events: 3149 mean_circ: 0.92563
Ran for 57550 frames Cell Events: 3207 mean_circ: 0.925745
Ran for 59550 frames Cell Events: 3328 mean_circ: 0.925708
Ran for 60050 frames Cell Events: 3349 mean_circ: 0.925794
Ran for 60550 frames Cell Events: 3374 mean_circ: 0.925806
Ran for 61550 frames Cell Events: 3430 mean_circ: 0.925823
Ran for 62050 frames Cell Events: 3461 mean_circ: 0.925857
Ran for 62550 frames Cell Events: 3477 mean_circ: 0.925855
Ran for 64550 frames Cell Events: 3568 mean_circ: 0.925973
Ran for 65050 frames Cell Events: 3606 mean_circ: 0.925973
Ran for 65550 frames Cell Events: 3640 mean_circ: 0.925946
Ran for 66550 frames Cell Events: 3697 mean_circ: 0.925765
Ran for 67550 frames Cell Events: 3767 mean_circ: 0.925706
Ran for 68550 frames Cell Events: 3820 mean_circ: 0.925836
Ran for 70550 frames Cell Events: 3928 mean_circ: 0.925891
Ran for 71550 frames Cell Events: 3982 mean_circ: 0.925922
Ran for 72050 frames Cell Events: 4006 mean_circ: 0.925884
Ran for 72550 frames Cell Events: 4039 mean_circ: 0.925849
Ran for 73550 frames Cell Events: 4107 mean_circ: 0.925734
Ran for 74050 frames Cell Events: 4140 mean_circ: 0.925787
Ran for 74550 frames Cell Events: 4163 mean_circ: 0.925854
Ran for 75050 frames Cell Events: 4202 mean_circ: 0.925963
Ran for 75550 frames Cell Events: 4227 mean_circ: 0.926015
Ran for 76050 frames Cell Events: 4247 mean_circ: 0.926047
Ran for 76550 frames Cell Events: 4275 mean_circ: 0.926062
Ran for 77050 frames Cell Events: 4301 mean_circ: 0.92608
Ran for 77550 frames Cell Events: 4332 mean_circ: 0.926092
Ran for 78050 frames Cell Events: 4357 mean_circ: 0.926142
Ran for 78550 frames Cell Events: 4385 mean_circ: 0.926123
Ran for 79050 frames Cell Events: 4414 mean_circ: 0.926111
Ran for 79550 frames Cell Events: 4446 mean_circ: 0.926055
Ran for 80050 frames Cell Events: 4462 mean_circ: 0.926028
Ran for 81050 frames Cell Events: 4517 mean_circ: 0.926045
Ran for 81550 frames Cell Events: 4540 mean_circ: 0.925968
Ran for 82050 frames Cell Events: 4561 mean_circ: 0.925998
Ran for 82550 frames Cell Events: 4581 mean_circ: 0.925993
Ran for 83050 frames Cell Events: 4613 mean_circ: 0.925941
Ran for 83550 frames Cell Events: 4635 mean_circ: 0.92587
Ran for 84050 frames Cell Events: 4663 mean_circ: 0.925827
Ran for 84550 frames Cell Events: 4683 mean_circ: 0.925739
Ran for 85050 frames Cell Events: 4707 mean_circ: 0.925765
Ran for 85550 frames Cell Events: 4743 mean_circ: 0.925857
Ran for 86550 frames Cell Events: 4793 mean_circ: 0.925843
Ran for 87050 frames Cell Events: 4823 mean_circ: 0.925878
Ran for 88050 frames Cell Events: 4901 mean_circ: 0.925794
Ran for 89050 frames Cell Events: 4963 mean_circ: 0.925741
Ran for 90050 frames Cell Events: 5014 mean_circ: 0.925704
Ran for 90550 frames Cell Events: 5038 mean_circ: 0.925695
Ran for 91550 frames Cell Events: 5101 mean_circ: 0.92569
Ran for 92050 frames Cell Events: 5123 mean_circ: 0.925727
Ran for 92550 frames Cell Events: 5152 mean_circ: 0.92576
Ran for 94050 frames Cell Events: 5226 mean_circ: 0.925746
Ran for 95550 frames Cell Events: 5299 mean_circ: 0.925846
Ran for 96550 frames Cell Events: 5344 mean_circ: 0.925926
Ran for 97050 frames Cell Events: 5364 mean_circ: 0.925797
Ran for 97550 frames Cell Events: 5376 mean_circ: 0.925756
Ran for 101050 frames Cell Events: 5564 mean_circ: 0.925717
Ran for 102050 frames Cell Events: 5626 mean_circ: 0.925713
Ran for 102550 frames Cell Events: 5649 mean_circ: 0.925708
Ran for 103050 frames Cell Events: 5679 mean_circ: 0.925692
Ran for 103550 frames Cell Events: 5705 mean_circ: 0.925626
Ran for 105050 frames Cell Events: 5815 mean_circ: 0.925669
Ran for 105550 frames Cell Events: 5850 mean_circ: 0.925667
Ran for 107050 frames Cell Events: 5944 mean_circ: 0.925595
Ran for 109550 frames Cell Events: 6075 mean_circ: 0.925554
Ran for 110550 frames Cell Events: 6134 mean_circ: 0.92555
Ran for 112050 frames Cell Events: 6218 mean_circ: 0.925454
Ran for 112550 frames Cell Events: 6242 mean_circ: 0.925433
Ran for 113550 frames Cell Events: 6287 mean_circ: 0.925483
Ran for 114050 frames Cell Events: 6309 mean_circ: 0.925506
Ran for 115050 frames Cell Events: 6360 mean_circ: 0.925548
Ran for 115550 frames Cell Events: 6377 mean_circ: 0.925551
Ran for 116550 frames Cell Events: 6433 mean_circ: 0.925639
Ran for 117050 frames Cell Events: 6462 mean_circ: 0.925632
Ran for 117550 frames Cell Events: 6494 mean_circ: 0.925669
Ran for 119050 frames Cell Events: 6541 mean_circ: 0.925677
Ran for 119550 frames Cell Events: 6570 mean_circ: 0.925618
Ran for 120550 frames Cell Events: 6616 mean_circ: 0.925576
Ran for 121550 frames Cell Events: 6656 mean_circ: 0.925612
Ran for 124550 frames Cell Events: 6791 mean_circ: 0.925667
Ran for 125050 frames Cell Events: 6809 mean_circ: 0.925666
Ran for 126050 frames Cell Events: 6858 mean_circ: 0.925714
Ran for 127050 frames Cell Events: 6915 mean_circ: 0.925672
Ran for 127550 frames Cell Events: 6938 mean_circ: 0.925644
Ran for 129550 frames Cell Events: 7022 mean_circ: 0.925635
Ran for 130550 frames Cell Events: 7080 mean_circ: 0.925629
Ran for 131550 frames Cell Events: 7132 mean_circ: 0.925631
Ran for 132050 frames Cell Events: 7149 mean_circ: 0.925581
Ran for 133550 frames Cell Events: 7259 mean_circ: 0.925548
Ran for 134550 frames Cell Events: 7315 mean_circ: 0.925465
Ran for 137550 frames Cell Events: 7459 mean_circ: 0.925356
Ran for 138050 frames Cell Events: 7479 mean_circ: 0.925264
Ran for 140550 frames Cell Events: 7635 mean_circ: 0.925431
Ran for 144050 frames Cell Events: 7831 mean_circ: 0.92528
Ran for 145050 frames Cell Events: 7882 mean_circ: 0.925308
Ran for 145550 frames Cell Events: 7907 mean_circ: 0.925265
Ran for 146550 frames Cell Events: 7962 mean_circ: 0.925317
Ran for 147050 frames Cell Events: 7983 mean_circ: 0.925294
Ran for 148050 frames Cell Events: 8043 mean_circ: 0.925226
Ran for 148550 frames Cell Events: 8072 mean_circ: 0.925214
Ran for 149050 frames Cell Events: 8096 mean_circ: 0.925202
Ran for 149550 frames Cell Events: 8109 mean_circ: 0.925229
Ran for 150050 frames Cell Events: 8128 mean_circ: 0.92519
Ran for 150550 frames Cell Events: 8145 mean_circ: 0.925199
Ran for 152550 frames Cell Events: 8278 mean_circ: 0.925233
Ran for 153050 frames Cell Events: 8314 mean_circ: 0.925227
Ran for 154050 frames Cell Events: 8376 mean_circ: 0.925199
Ran for 154550 frames Cell Events: 8395 mean_circ: 0.925201
Ran for 155050 frames Cell Events: 8426 mean_circ: 0.925201
Ran for 155550 frames Cell Events: 8462 mean_circ: 0.925252
Ran for 156050 frames Cell Events: 8487 mean_circ: 0.925258
Ran for 157050 frames Cell Events: 8552 mean_circ: 0.925297
Ran for 157550 frames Cell Events: 8584 mean_circ: 0.925256
Ran for 158550 frames Cell Events: 8656 mean_circ: 0.925255
Ran for 159050 frames Cell Events: 8674 mean_circ: 0.925249
Ran for 159550 frames Cell Events: 8700 mean_circ: 0.925244
Ran for 160050 frames Cell Events: 8721 mean_circ: 0.925208
Ran for 161050 frames Cell Events: 8758 mean_circ: 0.925215
Ran for 163050 frames Cell Events: 8889 mean_circ: 0.925228
Ran for 163550 frames Cell Events: 8919 mean_circ: 0.925281
Ran for 164050 frames Cell Events: 8943 mean_circ: 0.925245
Ran for 165550 frames Cell Events: 9036 mean_circ: 0.925249
Ran for 166550 frames Cell Events: 9088 mean_circ: 0.925225
Ran for 167050 frames Cell Events: 9121 mean_circ: 0.925213
Ran for 169050 frames Cell Events: 9270 mean_circ: 0.925151
Ran for 170050 frames Cell Events: 9312 mean_circ: 0.925164
Ran for 171550 frames Cell Events: 9391 mean_circ: 0.925131
Ran for 172550 frames Cell Events: 9414 mean_circ: 0.925152
Ran for 173050 frames Cell Events: 9439 mean_circ: 0.925154
Ran for 173550 frames Cell Events: 9469 mean_circ: 0.925174
Ran for 174550 frames Cell Events: 9522 mean_circ: 0.925131
Ran for 175050 frames Cell Events: 9551 mean_circ: 0.92511
Ran for 175550 frames Cell Events: 9573 mean_circ: 0.925127
Ran for 176050 frames Cell Events: 9592 mean_circ: 0.92513
Ran for 176550 frames Cell Events: 9615 mean_circ: 0.925122
End this measurment..
Stopping time: 6.40432e+008
****************************************
Measurement finished
Mean Circularitiy: 0.925108
Mean Area: 656.65
Mean FL-1 intensity: 2854.79
Mean FL-2 intensity: 4242.61
Mean FL-3 intensity: 0
frames processed: 173021
Total Time: 88430ms
Number of Runs (new bg img) 0
Frames collected: 176711
Skipped frames: 96539
Rescued Frames: 4548820
Samples DAQ: 44178916
Samples written: 88355542
Samples per Channel: 44177771
FPS: 1998.31
Samples per second: 499579
Expected samples/s: 500000
Events: 9621
Events per second: 108.798
jumped back (PMT): 18
#Frames read, frequency
# 0 frames :262650
# 1 frames :76263
# 2 frames :3648
# 3 frames :1050
# 4 frames :530
# 5 frames :205
# 6 frames :3171
# 7 frames :1424
# 8 frames :1354
# 9 frames :629
# 10 frames :427
# 11 frames :228
# 12 frames :252
# 13 frames :181
# 14 frames :327
# 15 frames :192
# 16 frames :181
# 17 frames :106
# 18 frames :80
# 19 frames :43
# 20 frames :38
# 21 frames :68
# 22 frames :48
# 23 frames :38
# 24 frames :31
# 25 frames :21
# 26 frames :17
# 27 frames :22
# 28 frames :22
# 29 frames :23
# 30 frames :12
# 31 frames :13
# 32 frames :11
# 33 frames :8
# 34 frames :5
# 35 frames :4
# 36 frames :3
# 37 frames :3
# 38 frames :2
# 39 frames :3
# 40 frames :2
# 41 frames :1
# 42 frames :4
# 43 frames :4
# 44 frames :4
# 45 frames :1
# 46 frames :4
# 47 frames :1
# 48 frames :3
# 49 frames :2
# 50 frames :3
# 51 frames :3
# 54 frames :1
# 56 frames :2
# 66 frames :1
# 87 frames :1
M1_para.ini
[General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100
[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0
#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 25.3934
parameters.txt
#fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0