Click on the log titles below to expand the log entries present in this DC resource.
dclab-export_2024-07-09_19.48.42
{"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc
{
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "fc10f560b8a619794aeb1f51629195fe",
"name": "M1_20170523_1404_dsRNA_G4_faster.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-09",
"time": "17:49:03"
}
}
dclab-tdms2rtdc-warnings
LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8392 (max 8393) in '8392'.
FG_Config.mcf
[Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;
[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;
[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini
[Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6
[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini
[EVENT LOG] fRT-DC V1.06170206
Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 24.9076
Start pulse train for BG Aquisition..
Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 7.11692e+008
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 550 frames Cell Events: 31 mean_circ: 0.887541
Ran for 1050 frames Cell Events: 58 mean_circ: 0.932607
Ran for 1550 frames Cell Events: 99 mean_circ: 0.933435
Ran for 2050 frames Cell Events: 119 mean_circ: 0.937049
Ran for 3550 frames Cell Events: 211 mean_circ: 0.938022
Ran for 6550 frames Cell Events: 377 mean_circ: 0.936154
Ran for 7050 frames Cell Events: 397 mean_circ: 0.936461
Ran for 7550 frames Cell Events: 425 mean_circ: 0.936965
Ran for 8050 frames Cell Events: 448 mean_circ: 0.936521
Ran for 9050 frames Cell Events: 520 mean_circ: 0.936957
Ran for 9550 frames Cell Events: 553 mean_circ: 0.936277
Ran for 10050 frames Cell Events: 583 mean_circ: 0.936481
Ran for 10550 frames Cell Events: 610 mean_circ: 0.936361
Ran for 12550 frames Cell Events: 734 mean_circ: 0.936755
Ran for 13050 frames Cell Events: 756 mean_circ: 0.936792
Ran for 14050 frames Cell Events: 818 mean_circ: 0.936358
Ran for 16050 frames Cell Events: 942 mean_circ: 0.936161
Ran for 16550 frames Cell Events: 961 mean_circ: 0.936131
Ran for 17050 frames Cell Events: 983 mean_circ: 0.936227
Ran for 18050 frames Cell Events: 1036 mean_circ: 0.935888
Ran for 18550 frames Cell Events: 1068 mean_circ: 0.935342
Ran for 19050 frames Cell Events: 1090 mean_circ: 0.935106
Ran for 20550 frames Cell Events: 1182 mean_circ: 0.935253
Ran for 21550 frames Cell Events: 1242 mean_circ: 0.935052
Ran for 22050 frames Cell Events: 1265 mean_circ: 0.934992
Ran for 24050 frames Cell Events: 1419 mean_circ: 0.935363
Ran for 26050 frames Cell Events: 1547 mean_circ: 0.93558
Ran for 27550 frames Cell Events: 1632 mean_circ: 0.935448
Ran for 28550 frames Cell Events: 1692 mean_circ: 0.934975
Ran for 29050 frames Cell Events: 1724 mean_circ: 0.934906
Ran for 29550 frames Cell Events: 1744 mean_circ: 0.934925
Ran for 30050 frames Cell Events: 1780 mean_circ: 0.934717
Ran for 30550 frames Cell Events: 1810 mean_circ: 0.934732
Ran for 32050 frames Cell Events: 1893 mean_circ: 0.934642
Ran for 34050 frames Cell Events: 2011 mean_circ: 0.934607
Ran for 34550 frames Cell Events: 2045 mean_circ: 0.93451
Ran for 35050 frames Cell Events: 2085 mean_circ: 0.934499
Ran for 36050 frames Cell Events: 2131 mean_circ: 0.934377
Ran for 36550 frames Cell Events: 2158 mean_circ: 0.934462
Ran for 37050 frames Cell Events: 2192 mean_circ: 0.934547
Ran for 38050 frames Cell Events: 2254 mean_circ: 0.934772
Ran for 38550 frames Cell Events: 2279 mean_circ: 0.934806
Ran for 40550 frames Cell Events: 2404 mean_circ: 0.934967
Ran for 41550 frames Cell Events: 2458 mean_circ: 0.934982
Ran for 44550 frames Cell Events: 2649 mean_circ: 0.935161
Ran for 45050 frames Cell Events: 2676 mean_circ: 0.935021
Ran for 45550 frames Cell Events: 2704 mean_circ: 0.93514
Ran for 46050 frames Cell Events: 2726 mean_circ: 0.935171
Ran for 46550 frames Cell Events: 2761 mean_circ: 0.935177
Ran for 47050 frames Cell Events: 2791 mean_circ: 0.935304
Ran for 48550 frames Cell Events: 2886 mean_circ: 0.93507
Ran for 49050 frames Cell Events: 2907 mean_circ: 0.934996
Ran for 50050 frames Cell Events: 2980 mean_circ: 0.934857
Ran for 51050 frames Cell Events: 3070 mean_circ: 0.934922
Ran for 51550 frames Cell Events: 3099 mean_circ: 0.934667
Ran for 52050 frames Cell Events: 3123 mean_circ: 0.934669
Ran for 52550 frames Cell Events: 3156 mean_circ: 0.934709
Ran for 53550 frames Cell Events: 3206 mean_circ: 0.934759
Ran for 55050 frames Cell Events: 3293 mean_circ: 0.934722
Ran for 55550 frames Cell Events: 3311 mean_circ: 0.93476
Ran for 56550 frames Cell Events: 3375 mean_circ: 0.934732
Ran for 57550 frames Cell Events: 3424 mean_circ: 0.934773
Ran for 58050 frames Cell Events: 3453 mean_circ: 0.934814
Ran for 60050 frames Cell Events: 3586 mean_circ: 0.935041
Ran for 61050 frames Cell Events: 3642 mean_circ: 0.935012
Ran for 62050 frames Cell Events: 3701 mean_circ: 0.935035
Ran for 62550 frames Cell Events: 3722 mean_circ: 0.935028
Ran for 63050 frames Cell Events: 3751 mean_circ: 0.934949
Ran for 63550 frames Cell Events: 3769 mean_circ: 0.934978
Ran for 66050 frames Cell Events: 3888 mean_circ: 0.934763
Ran for 67550 frames Cell Events: 3962 mean_circ: 0.934698
Ran for 68050 frames Cell Events: 3989 mean_circ: 0.934672
Ran for 69050 frames Cell Events: 4038 mean_circ: 0.934643
Ran for 70050 frames Cell Events: 4086 mean_circ: 0.934619
Ran for 70550 frames Cell Events: 4115 mean_circ: 0.934694
Ran for 71050 frames Cell Events: 4140 mean_circ: 0.934727
Ran for 71550 frames Cell Events: 4173 mean_circ: 0.934739
Ran for 72050 frames Cell Events: 4202 mean_circ: 0.934749
Ran for 72550 frames Cell Events: 4232 mean_circ: 0.934764
Ran for 74050 frames Cell Events: 4315 mean_circ: 0.934781
Ran for 75050 frames Cell Events: 4371 mean_circ: 0.934862
Ran for 77550 frames Cell Events: 4509 mean_circ: 0.934822
Ran for 78050 frames Cell Events: 4538 mean_circ: 0.934844
Ran for 80050 frames Cell Events: 4636 mean_circ: 0.934801
Ran for 82550 frames Cell Events: 4787 mean_circ: 0.934808
Ran for 83050 frames Cell Events: 4809 mean_circ: 0.934821
Ran for 85050 frames Cell Events: 4919 mean_circ: 0.934879
Ran for 88550 frames Cell Events: 5143 mean_circ: 0.934862
Ran for 89050 frames Cell Events: 5167 mean_circ: 0.934841
Ran for 90050 frames Cell Events: 5216 mean_circ: 0.934837
Ran for 90550 frames Cell Events: 5257 mean_circ: 0.934867
Ran for 91050 frames Cell Events: 5288 mean_circ: 0.934853
Ran for 91550 frames Cell Events: 5318 mean_circ: 0.934738
Ran for 93050 frames Cell Events: 5408 mean_circ: 0.934703
Ran for 93550 frames Cell Events: 5438 mean_circ: 0.934654
Ran for 95050 frames Cell Events: 5529 mean_circ: 0.934629
Ran for 96550 frames Cell Events: 5627 mean_circ: 0.934626
Ran for 97050 frames Cell Events: 5660 mean_circ: 0.93471
Ran for 97550 frames Cell Events: 5692 mean_circ: 0.934757
Ran for 98050 frames Cell Events: 5714 mean_circ: 0.934893
Ran for 98550 frames Cell Events: 5747 mean_circ: 0.93488
Ran for 99050 frames Cell Events: 5774 mean_circ: 0.934842
Ran for 100050 frames Cell Events: 5821 mean_circ: 0.934816
Ran for 101050 frames Cell Events: 5880 mean_circ: 0.934852
Ran for 101550 frames Cell Events: 5907 mean_circ: 0.934896
Ran for 102550 frames Cell Events: 5951 mean_circ: 0.934825
Ran for 103050 frames Cell Events: 5974 mean_circ: 0.934784
Ran for 104550 frames Cell Events: 6068 mean_circ: 0.934768
Ran for 105050 frames Cell Events: 6097 mean_circ: 0.934728
Ran for 105550 frames Cell Events: 6138 mean_circ: 0.934709
Ran for 106050 frames Cell Events: 6170 mean_circ: 0.934703
Ran for 106550 frames Cell Events: 6194 mean_circ: 0.934694
Ran for 107050 frames Cell Events: 6224 mean_circ: 0.934675
Ran for 108550 frames Cell Events: 6308 mean_circ: 0.934712
Ran for 109550 frames Cell Events: 6351 mean_circ: 0.934733
Ran for 111050 frames Cell Events: 6465 mean_circ: 0.934507
Ran for 112050 frames Cell Events: 6533 mean_circ: 0.934503
Ran for 113050 frames Cell Events: 6593 mean_circ: 0.93453
Ran for 114050 frames Cell Events: 6645 mean_circ: 0.934586
Ran for 114550 frames Cell Events: 6676 mean_circ: 0.934582
Ran for 115550 frames Cell Events: 6745 mean_circ: 0.934567
Ran for 117550 frames Cell Events: 6874 mean_circ: 0.934601
Ran for 118050 frames Cell Events: 6912 mean_circ: 0.934604
Ran for 118550 frames Cell Events: 6945 mean_circ: 0.93458
Ran for 119050 frames Cell Events: 6974 mean_circ: 0.934584
Ran for 120050 frames Cell Events: 7044 mean_circ: 0.934597
Ran for 120550 frames Cell Events: 7074 mean_circ: 0.934537
Ran for 121550 frames Cell Events: 7127 mean_circ: 0.93458
Ran for 122550 frames Cell Events: 7180 mean_circ: 0.934579
Ran for 124050 frames Cell Events: 7263 mean_circ: 0.934587
Ran for 125050 frames Cell Events: 7324 mean_circ: 0.934674
Ran for 125550 frames Cell Events: 7352 mean_circ: 0.934637
Ran for 126550 frames Cell Events: 7419 mean_circ: 0.934546
Ran for 128050 frames Cell Events: 7495 mean_circ: 0.934589
Ran for 128550 frames Cell Events: 7516 mean_circ: 0.934567
Ran for 130550 frames Cell Events: 7605 mean_circ: 0.934527
Ran for 132550 frames Cell Events: 7709 mean_circ: 0.934609
Ran for 133550 frames Cell Events: 7772 mean_circ: 0.934519
Ran for 134050 frames Cell Events: 7786 mean_circ: 0.934539
Ran for 135050 frames Cell Events: 7837 mean_circ: 0.934505
Ran for 135550 frames Cell Events: 7875 mean_circ: 0.934525
Ran for 138550 frames Cell Events: 8069 mean_circ: 0.934597
Ran for 139050 frames Cell Events: 8092 mean_circ: 0.934611
Ran for 141550 frames Cell Events: 8209 mean_circ: 0.934632
Ran for 144050 frames Cell Events: 8385 mean_circ: 0.934691
End this measurment..
Stopping time: 7.11764e+008
****************************************
Measurement finished
Mean Circularitiy: 0.93469
Mean Area: 599.921
Mean FL-1 intensity: 3318.27
Mean FL-2 intensity: 4478.51
Mean FL-3 intensity: 0
frames processed: 141732
Total Time: 72152.6ms
Number of Runs (new bg img) 0
Frames collected: 144192
Skipped frames: 19134
Rescued Frames: 708260
Samples DAQ: 36049028
Samples written: 72095890
Samples per Channel: 36047945
FPS: 1998.43
Samples per second: 499607
Expected samples/s: 500000
Events: 8393
Events per second: 116.323
jumped back (PMT): 17
#Frames read, frequency
# 0 frames :221925
# 1 frames :54460
# 2 frames :2738
# 3 frames :925
# 4 frames :497
# 5 frames :214
# 6 frames :2316
# 7 frames :1095
# 8 frames :1065
# 9 frames :529
# 10 frames :349
# 11 frames :247
# 12 frames :211
# 13 frames :231
# 14 frames :268
# 15 frames :186
# 16 frames :138
# 17 frames :133
# 18 frames :92
# 19 frames :65
# 20 frames :75
# 21 frames :56
# 22 frames :62
# 23 frames :54
# 24 frames :33
# 25 frames :29
# 26 frames :29
# 27 frames :27
# 28 frames :18
# 29 frames :17
# 30 frames :22
# 31 frames :11
# 32 frames :19
# 33 frames :14
# 34 frames :12
# 35 frames :10
# 36 frames :12
# 37 frames :11
# 38 frames :9
# 39 frames :6
# 40 frames :6
# 41 frames :5
# 42 frames :4
# 43 frames :6
# 44 frames :6
# 46 frames :5
# 48 frames :4
# 49 frames :3
# 50 frames :5
# 51 frames :2
# 52 frames :3
# 53 frames :2
# 54 frames :1
# 55 frames :2
# 56 frames :2
# 57 frames :1
# 58 frames :3
# 61 frames :3
# 64 frames :1
# 66 frames :1
# 67 frames :1
# 74 frames :1
# 98 frames :1
M1_para.ini
[General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100
[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0
#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 24.9076
parameters.txt
#fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0