Click on the log titles below to expand the log entries present in this DC resource.
dclab-export_2024-07-09_19.50.56
{"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc
{
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "8903c44208e23cdf86e38ea2de45d4a0",
"name": "M1_20170523_1436_dsRNA_F7_col.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-09",
"time": "17:51:16"
}
}
dclab-tdms2rtdc-warnings
LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8146 (max 8147) in '8146'.
FG_Config.mcf
[Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;
[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;
[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini
[Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6
[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini
[EVENT LOG] fRT-DC V1.06170206
Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 25.0562
Start pulse train for BG Aquisition..
Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 7.13652e+008
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 1050 frames Cell Events: 42 mean_circ: 0.941888
Ran for 1550 frames Cell Events: 62 mean_circ: 0.945265
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Ran for 3050 frames Cell Events: 118 mean_circ: 0.948144
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Ran for 5050 frames Cell Events: 196 mean_circ: 0.945927
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Ran for 77550 frames Cell Events: 2865 mean_circ: 0.945698
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Ran for 83050 frames Cell Events: 3087 mean_circ: 0.945943
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Ran for 85050 frames Cell Events: 3159 mean_circ: 0.946018
Ran for 85550 frames Cell Events: 3174 mean_circ: 0.946063
Ran for 86050 frames Cell Events: 3186 mean_circ: 0.946071
Ran for 87050 frames Cell Events: 3219 mean_circ: 0.946134
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Ran for 92050 frames Cell Events: 3413 mean_circ: 0.946302
Ran for 92550 frames Cell Events: 3426 mean_circ: 0.946321
Ran for 93050 frames Cell Events: 3457 mean_circ: 0.946153
Ran for 93550 frames Cell Events: 3478 mean_circ: 0.946177
Ran for 96050 frames Cell Events: 3670 mean_circ: 0.945518
Ran for 96550 frames Cell Events: 3698 mean_circ: 0.945524
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Ran for 97550 frames Cell Events: 3751 mean_circ: 0.945497
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Ran for 121050 frames Cell Events: 4785 mean_circ: 0.945781
Ran for 121550 frames Cell Events: 4811 mean_circ: 0.945818
Ran for 122050 frames Cell Events: 4830 mean_circ: 0.945863
Ran for 122550 frames Cell Events: 4849 mean_circ: 0.9458
Ran for 123550 frames Cell Events: 4886 mean_circ: 0.945825
Ran for 124050 frames Cell Events: 4898 mean_circ: 0.94583
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Ran for 125050 frames Cell Events: 4935 mean_circ: 0.945888
Ran for 125550 frames Cell Events: 4954 mean_circ: 0.9459
Ran for 126550 frames Cell Events: 5000 mean_circ: 0.945929
Ran for 127550 frames Cell Events: 5040 mean_circ: 0.945978
Ran for 128550 frames Cell Events: 5089 mean_circ: 0.94606
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Ran for 132550 frames Cell Events: 5290 mean_circ: 0.946099
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Ran for 142050 frames Cell Events: 5660 mean_circ: 0.946353
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Ran for 189050 frames Cell Events: 7717 mean_circ: 0.94639
Ran for 189550 frames Cell Events: 7732 mean_circ: 0.946411
Ran for 190550 frames Cell Events: 7777 mean_circ: 0.94641
Ran for 191050 frames Cell Events: 7798 mean_circ: 0.946397
Ran for 191550 frames Cell Events: 7821 mean_circ: 0.946345
Ran for 192050 frames Cell Events: 7841 mean_circ: 0.946324
Ran for 192550 frames Cell Events: 7862 mean_circ: 0.946362
Ran for 193050 frames Cell Events: 7884 mean_circ: 0.946341
Ran for 194050 frames Cell Events: 7931 mean_circ: 0.946381
Ran for 195050 frames Cell Events: 7975 mean_circ: 0.946417
Ran for 195550 frames Cell Events: 7994 mean_circ: 0.946425
Ran for 196550 frames Cell Events: 8048 mean_circ: 0.946411
Ran for 197550 frames Cell Events: 8098 mean_circ: 0.946397
Ran for 198050 frames Cell Events: 8116 mean_circ: 0.946392
End this measurment..
Stopping time: 7.13752e+008
****************************************
Measurement finished
Mean Circularitiy: 0.946405
Mean Area: 588.261
Mean FL-1 intensity: 4847.03
Mean FL-2 intensity: 6239.22
Mean FL-3 intensity: 0
frames processed: 197143
Total Time: 99263.4ms
Number of Runs (new bg img) 0
Frames collected: 198397
Skipped frames: 28206
Rescued Frames: 1131592
Samples DAQ: 49599418
Samples written: 99198286
Samples per Channel: 49599143
FPS: 1998.69
Samples per second: 499672
Expected samples/s: 500000
Events: 8147
Events per second: 82.0746
jumped back (PMT): 4912
#Frames read, frequency
# 0 frames :280128
# 1 frames :100560
# 2 frames :4460
# 3 frames :1379
# 4 frames :728
# 5 frames :291
# 6 frames :2158
# 7 frames :1889
# 8 frames :1120
# 9 frames :574
# 10 frames :523
# 11 frames :695
# 12 frames :359
# 13 frames :220
# 14 frames :195
# 15 frames :175
# 16 frames :136
# 17 frames :102
# 18 frames :77
# 19 frames :53
# 20 frames :58
# 21 frames :47
# 22 frames :24
# 23 frames :33
# 24 frames :21
# 25 frames :21
# 26 frames :14
# 27 frames :9
# 28 frames :7
# 29 frames :8
# 30 frames :8
# 31 frames :7
# 32 frames :4
# 33 frames :2
# 34 frames :3
# 35 frames :5
# 36 frames :2
# 38 frames :3
# 39 frames :5
# 41 frames :1
# 42 frames :2
# 43 frames :1
# 44 frames :1
# 45 frames :1
# 47 frames :1
# 50 frames :1
# 52 frames :1
# 55 frames :1
# 60 frames :1
M1_para.ini
[General]
Measurement Number = 1
Flow Rate [ul/s] = 0.04
Region = "Channel"
Channel width = 20
Current LED = 100
[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0
#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 25.0562
parameters.txt
#fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 300
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0