Click on the log titles below to expand the log entries present in this DC resource.
dclab-export_2024-07-09_19.52.26
{"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc
{
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "d36fb31e4b7cbaf3198dc5d99b9b8d10",
"name": "M1_20170523_1452_dsRNA_G3_faster.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-09",
"time": "17:52:52"
}
}
dclab-tdms2rtdc-warnings
LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 10000 (max 10001) in
'10000'.
FG_Config.mcf
[Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;
[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;
[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini
[Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6
[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini
[EVENT LOG] fRT-DC V1.06170206
Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 24.9941
Start pulse train for BG Aquisition..
Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 7.14572e+008
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 50 frames Cell Events: 1 mean_circ: 0.947747
Ran for 550 frames Cell Events: 22 mean_circ: 0.876592
Ran for 1050 frames Cell Events: 44 mean_circ: 0.918146
Ran for 3550 frames Cell Events: 168 mean_circ: 0.937802
Ran for 6050 frames Cell Events: 314 mean_circ: 0.936756
Ran for 7550 frames Cell Events: 392 mean_circ: 0.938243
Ran for 8050 frames Cell Events: 423 mean_circ: 0.93774
Ran for 8550 frames Cell Events: 437 mean_circ: 0.937915
Ran for 9050 frames Cell Events: 480 mean_circ: 0.9379
Ran for 9550 frames Cell Events: 502 mean_circ: 0.937969
Ran for 12050 frames Cell Events: 643 mean_circ: 0.936867
Ran for 12550 frames Cell Events: 673 mean_circ: 0.935658
Ran for 13050 frames Cell Events: 695 mean_circ: 0.935415
Ran for 14050 frames Cell Events: 743 mean_circ: 0.935125
Ran for 14550 frames Cell Events: 769 mean_circ: 0.935813
Ran for 15050 frames Cell Events: 794 mean_circ: 0.935778
Ran for 15550 frames Cell Events: 814 mean_circ: 0.935466
Ran for 16050 frames Cell Events: 838 mean_circ: 0.935281
Ran for 16550 frames Cell Events: 864 mean_circ: 0.935168
Ran for 18550 frames Cell Events: 988 mean_circ: 0.934188
Ran for 21550 frames Cell Events: 1165 mean_circ: 0.935368
Ran for 23050 frames Cell Events: 1258 mean_circ: 0.935377
Ran for 23550 frames Cell Events: 1285 mean_circ: 0.935428
Ran for 24550 frames Cell Events: 1332 mean_circ: 0.935831
Ran for 25550 frames Cell Events: 1373 mean_circ: 0.935858
Ran for 26050 frames Cell Events: 1400 mean_circ: 0.935897
Ran for 26550 frames Cell Events: 1417 mean_circ: 0.935792
Ran for 27550 frames Cell Events: 1464 mean_circ: 0.936167
Ran for 28050 frames Cell Events: 1494 mean_circ: 0.936105
Ran for 28550 frames Cell Events: 1514 mean_circ: 0.936333
Ran for 29550 frames Cell Events: 1580 mean_circ: 0.936027
Ran for 30050 frames Cell Events: 1622 mean_circ: 0.935977
Ran for 31050 frames Cell Events: 1685 mean_circ: 0.93598
Ran for 31550 frames Cell Events: 1713 mean_circ: 0.936088
Ran for 32050 frames Cell Events: 1729 mean_circ: 0.936257
Ran for 32550 frames Cell Events: 1746 mean_circ: 0.936338
Ran for 33050 frames Cell Events: 1783 mean_circ: 0.936296
Ran for 34550 frames Cell Events: 1841 mean_circ: 0.936552
Ran for 35050 frames Cell Events: 1858 mean_circ: 0.93655
Ran for 36050 frames Cell Events: 1909 mean_circ: 0.936776
Ran for 37050 frames Cell Events: 1967 mean_circ: 0.936791
Ran for 37550 frames Cell Events: 2003 mean_circ: 0.936715
Ran for 38550 frames Cell Events: 2057 mean_circ: 0.936931
Ran for 39050 frames Cell Events: 2089 mean_circ: 0.936891
Ran for 40550 frames Cell Events: 2174 mean_circ: 0.937087
Ran for 41050 frames Cell Events: 2198 mean_circ: 0.937106
Ran for 42050 frames Cell Events: 2243 mean_circ: 0.937253
Ran for 42550 frames Cell Events: 2268 mean_circ: 0.937221
Ran for 43050 frames Cell Events: 2295 mean_circ: 0.93711
Ran for 43550 frames Cell Events: 2326 mean_circ: 0.937158
Ran for 44550 frames Cell Events: 2380 mean_circ: 0.937248
Ran for 45050 frames Cell Events: 2408 mean_circ: 0.937107
Ran for 45550 frames Cell Events: 2445 mean_circ: 0.93726
Ran for 46050 frames Cell Events: 2476 mean_circ: 0.937401
Ran for 46550 frames Cell Events: 2507 mean_circ: 0.937381
Ran for 47050 frames Cell Events: 2550 mean_circ: 0.937436
Ran for 47550 frames Cell Events: 2581 mean_circ: 0.937463
Ran for 48050 frames Cell Events: 2601 mean_circ: 0.937433
Ran for 48550 frames Cell Events: 2641 mean_circ: 0.937531
Ran for 49050 frames Cell Events: 2671 mean_circ: 0.937497
Ran for 49550 frames Cell Events: 2707 mean_circ: 0.937488
Ran for 50050 frames Cell Events: 2743 mean_circ: 0.937475
Ran for 51550 frames Cell Events: 2852 mean_circ: 0.937473
Ran for 52550 frames Cell Events: 2905 mean_circ: 0.937426
Ran for 53550 frames Cell Events: 2975 mean_circ: 0.937348
Ran for 57050 frames Cell Events: 3213 mean_circ: 0.937534
Ran for 57550 frames Cell Events: 3257 mean_circ: 0.937604
Ran for 58550 frames Cell Events: 3315 mean_circ: 0.937586
Ran for 60550 frames Cell Events: 3429 mean_circ: 0.937559
Ran for 61050 frames Cell Events: 3465 mean_circ: 0.937411
Ran for 61550 frames Cell Events: 3499 mean_circ: 0.937417
Ran for 62050 frames Cell Events: 3519 mean_circ: 0.937546
Ran for 62550 frames Cell Events: 3545 mean_circ: 0.93749
Ran for 63550 frames Cell Events: 3624 mean_circ: 0.937576
Ran for 64050 frames Cell Events: 3641 mean_circ: 0.937642
Ran for 64550 frames Cell Events: 3675 mean_circ: 0.937719
Ran for 65050 frames Cell Events: 3700 mean_circ: 0.937714
Ran for 66050 frames Cell Events: 3741 mean_circ: 0.937587
Ran for 66550 frames Cell Events: 3771 mean_circ: 0.937601
Ran for 67050 frames Cell Events: 3795 mean_circ: 0.937486
Ran for 67550 frames Cell Events: 3813 mean_circ: 0.937529
Ran for 68050 frames Cell Events: 3853 mean_circ: 0.937529
Ran for 68550 frames Cell Events: 3885 mean_circ: 0.937492
Ran for 69050 frames Cell Events: 3916 mean_circ: 0.937419
Ran for 70050 frames Cell Events: 3950 mean_circ: 0.937432
Ran for 71050 frames Cell Events: 4019 mean_circ: 0.937391
Ran for 71550 frames Cell Events: 4043 mean_circ: 0.937383
Ran for 74550 frames Cell Events: 4231 mean_circ: 0.937215
Ran for 76050 frames Cell Events: 4323 mean_circ: 0.937374
Ran for 76550 frames Cell Events: 4347 mean_circ: 0.93733
Ran for 77050 frames Cell Events: 4374 mean_circ: 0.937373
Ran for 77550 frames Cell Events: 4399 mean_circ: 0.937371
Ran for 78550 frames Cell Events: 4456 mean_circ: 0.937407
Ran for 79050 frames Cell Events: 4493 mean_circ: 0.937398
Ran for 79550 frames Cell Events: 4516 mean_circ: 0.937466
Ran for 83050 frames Cell Events: 4694 mean_circ: 0.937399
Ran for 85050 frames Cell Events: 4805 mean_circ: 0.937275
Ran for 85550 frames Cell Events: 4829 mean_circ: 0.93725
Ran for 87050 frames Cell Events: 4934 mean_circ: 0.937233
Ran for 88550 frames Cell Events: 4986 mean_circ: 0.937329
Ran for 89050 frames Cell Events: 5020 mean_circ: 0.937271
Ran for 89550 frames Cell Events: 5049 mean_circ: 0.937229
Ran for 90050 frames Cell Events: 5081 mean_circ: 0.937241
Ran for 90550 frames Cell Events: 5116 mean_circ: 0.937144
Ran for 91050 frames Cell Events: 5150 mean_circ: 0.937143
Ran for 92050 frames Cell Events: 5197 mean_circ: 0.93719
Ran for 93550 frames Cell Events: 5287 mean_circ: 0.937123
Ran for 94050 frames Cell Events: 5313 mean_circ: 0.93716
Ran for 95050 frames Cell Events: 5365 mean_circ: 0.937113
Ran for 95550 frames Cell Events: 5387 mean_circ: 0.9371
Ran for 96050 frames Cell Events: 5416 mean_circ: 0.93707
Ran for 97050 frames Cell Events: 5481 mean_circ: 0.937148
Ran for 98050 frames Cell Events: 5558 mean_circ: 0.936988
Ran for 99050 frames Cell Events: 5608 mean_circ: 0.936828
Ran for 103550 frames Cell Events: 5866 mean_circ: 0.936693
Ran for 104550 frames Cell Events: 5918 mean_circ: 0.936684
Ran for 106550 frames Cell Events: 6037 mean_circ: 0.936666
Ran for 108050 frames Cell Events: 6116 mean_circ: 0.93658
Ran for 108550 frames Cell Events: 6157 mean_circ: 0.936636
Ran for 110550 frames Cell Events: 6277 mean_circ: 0.936583
Ran for 111050 frames Cell Events: 6315 mean_circ: 0.93663
Ran for 112050 frames Cell Events: 6388 mean_circ: 0.936627
Ran for 112550 frames Cell Events: 6404 mean_circ: 0.936657
Ran for 113050 frames Cell Events: 6443 mean_circ: 0.936522
Ran for 114050 frames Cell Events: 6493 mean_circ: 0.936574
Ran for 115550 frames Cell Events: 6592 mean_circ: 0.936456
Ran for 116050 frames Cell Events: 6622 mean_circ: 0.936467
Ran for 117050 frames Cell Events: 6681 mean_circ: 0.936509
Ran for 118550 frames Cell Events: 6779 mean_circ: 0.936512
Ran for 120050 frames Cell Events: 6878 mean_circ: 0.936649
Ran for 122050 frames Cell Events: 7001 mean_circ: 0.936593
Ran for 123050 frames Cell Events: 7069 mean_circ: 0.936542
Ran for 124050 frames Cell Events: 7133 mean_circ: 0.936493
Ran for 125550 frames Cell Events: 7228 mean_circ: 0.936497
Ran for 126550 frames Cell Events: 7323 mean_circ: 0.936603
Ran for 128550 frames Cell Events: 7452 mean_circ: 0.936615
Ran for 130050 frames Cell Events: 7555 mean_circ: 0.936668
Ran for 131550 frames Cell Events: 7649 mean_circ: 0.936715
Ran for 132550 frames Cell Events: 7727 mean_circ: 0.936625
Ran for 133050 frames Cell Events: 7768 mean_circ: 0.936609
Ran for 133550 frames Cell Events: 7808 mean_circ: 0.936611
Ran for 134050 frames Cell Events: 7834 mean_circ: 0.936578
Ran for 134550 frames Cell Events: 7852 mean_circ: 0.936604
Ran for 135050 frames Cell Events: 7885 mean_circ: 0.93664
Ran for 135550 frames Cell Events: 7909 mean_circ: 0.93664
Ran for 136050 frames Cell Events: 7933 mean_circ: 0.936617
Ran for 139550 frames Cell Events: 8174 mean_circ: 0.936595
Ran for 140050 frames Cell Events: 8196 mean_circ: 0.936588
Ran for 141050 frames Cell Events: 8250 mean_circ: 0.936537
Ran for 142050 frames Cell Events: 8315 mean_circ: 0.936645
Ran for 142550 frames Cell Events: 8334 mean_circ: 0.936659
Ran for 143550 frames Cell Events: 8399 mean_circ: 0.936632
Ran for 144050 frames Cell Events: 8428 mean_circ: 0.936645
Ran for 145050 frames Cell Events: 8487 mean_circ: 0.936594
Ran for 145550 frames Cell Events: 8508 mean_circ: 0.936619
Ran for 146550 frames Cell Events: 8563 mean_circ: 0.936606
Ran for 147050 frames Cell Events: 8583 mean_circ: 0.936591
Ran for 148050 frames Cell Events: 8641 mean_circ: 0.936518
Ran for 150050 frames Cell Events: 8754 mean_circ: 0.936556
Ran for 150550 frames Cell Events: 8776 mean_circ: 0.93657
Ran for 151050 frames Cell Events: 8820 mean_circ: 0.936597
Ran for 151550 frames Cell Events: 8861 mean_circ: 0.936597
Ran for 152050 frames Cell Events: 8892 mean_circ: 0.93657
Ran for 153050 frames Cell Events: 8936 mean_circ: 0.93656
Ran for 153550 frames Cell Events: 8973 mean_circ: 0.936555
Ran for 154050 frames Cell Events: 9004 mean_circ: 0.936594
Ran for 158050 frames Cell Events: 9291 mean_circ: 0.936517
Ran for 158550 frames Cell Events: 9326 mean_circ: 0.936475
Ran for 163050 frames Cell Events: 9651 mean_circ: 0.936461
Ran for 163550 frames Cell Events: 9680 mean_circ: 0.936493
Ran for 164050 frames Cell Events: 9717 mean_circ: 0.93646
Ran for 165050 frames Cell Events: 9769 mean_circ: 0.936491
Ran for 166050 frames Cell Events: 9830 mean_circ: 0.936486
Ran for 168050 frames Cell Events: 9936 mean_circ: 0.936506
Ran for 168550 frames Cell Events: 9969 mean_circ: 0.936538
End this measurment.
because stop condition (time/nr of cells) was met
Stopping time: 7.14657e+008
****************************************
Measurement finished
Mean Circularitiy: 0.936568
Mean Area: 575.637
Mean FL-1 intensity: 3203.97
Mean FL-2 intensity: 4268.77
Mean FL-3 intensity: 0
frames processed: 166825
Total Time: 84520.1ms
Number of Runs (new bg img) 0
Frames collected: 168948
Skipped frames: 33522
Rescued Frames: 1386869
Samples DAQ: 42238001
Samples written: 84473780
Samples per Channel: 42236890
FPS: 1998.91
Samples per second: 499726
Expected samples/s: 500000
Events: 10001
Events per second: 118.327
jumped back (PMT): 17
#Frames read, frequency
# 0 frames :257784
# 1 frames :66156
# 2 frames :3193
# 3 frames :1028
# 4 frames :584
# 5 frames :245
# 6 frames :2953
# 7 frames :1299
# 8 frames :1223
# 9 frames :623
# 10 frames :411
# 11 frames :293
# 12 frames :253
# 13 frames :199
# 14 frames :336
# 15 frames :207
# 16 frames :154
# 17 frames :115
# 18 frames :86
# 19 frames :61
# 20 frames :90
# 21 frames :77
# 22 frames :77
# 23 frames :50
# 24 frames :44
# 25 frames :37
# 26 frames :26
# 27 frames :44
# 28 frames :24
# 29 frames :35
# 30 frames :17
# 31 frames :17
# 32 frames :14
# 33 frames :17
# 34 frames :14
# 35 frames :11
# 36 frames :7
# 37 frames :4
# 38 frames :2
# 39 frames :7
# 40 frames :5
# 41 frames :12
# 42 frames :4
# 43 frames :5
# 44 frames :4
# 45 frames :5
# 46 frames :4
# 47 frames :3
# 48 frames :1
# 49 frames :3
# 50 frames :2
# 51 frames :1
# 52 frames :2
# 53 frames :3
# 54 frames :2
# 57 frames :1
# 58 frames :1
# 59 frames :1
# 63 frames :2
# 64 frames :2
# 71 frames :1
# 77 frames :1
# 79 frames :1
M1_para.ini
[General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100
[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0
#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 24.9941
parameters.txt
#fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0