Click on the log titles below to expand the log entries present in this DC resource.
dclab-export_2024-07-09_19.53.34
{"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc
{
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "b3ce36a3774d806e1a2c60de036c8b6b",
"name": "M1_20170523_1505_dsRNA_F7_faster.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-09",
"time": "17:53:54"
}
}
dclab-tdms2rtdc-warnings
LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8090 (max 8091) in '8090'.
FG_Config.mcf
[Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;
[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;
[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini
[Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6
[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini
[EVENT LOG] fRT-DC V1.06170206
Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 25.0972
Start pulse train for BG Aquisition..
Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 7.15347e+008
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 550 frames Cell Events: 19 mean_circ: 0.924309
Ran for 2050 frames Cell Events: 75 mean_circ: 0.93082
Ran for 3050 frames Cell Events: 136 mean_circ: 0.938121
Ran for 3550 frames Cell Events: 164 mean_circ: 0.937127
Ran for 5050 frames Cell Events: 224 mean_circ: 0.938325
Ran for 5550 frames Cell Events: 242 mean_circ: 0.93827
Ran for 6050 frames Cell Events: 269 mean_circ: 0.937898
Ran for 6550 frames Cell Events: 294 mean_circ: 0.938625
Ran for 7050 frames Cell Events: 325 mean_circ: 0.938579
Ran for 8050 frames Cell Events: 368 mean_circ: 0.937573
Ran for 10050 frames Cell Events: 469 mean_circ: 0.93457
Ran for 11550 frames Cell Events: 561 mean_circ: 0.93313
Ran for 12050 frames Cell Events: 577 mean_circ: 0.933249
Ran for 12550 frames Cell Events: 596 mean_circ: 0.933039
Ran for 13550 frames Cell Events: 655 mean_circ: 0.932449
Ran for 14050 frames Cell Events: 676 mean_circ: 0.932755
Ran for 15050 frames Cell Events: 717 mean_circ: 0.932392
Ran for 15550 frames Cell Events: 756 mean_circ: 0.93298
Ran for 16550 frames Cell Events: 794 mean_circ: 0.93336
Ran for 17050 frames Cell Events: 819 mean_circ: 0.933751
Ran for 18050 frames Cell Events: 869 mean_circ: 0.93392
Ran for 20550 frames Cell Events: 1019 mean_circ: 0.933867
Ran for 21050 frames Cell Events: 1056 mean_circ: 0.933445
Ran for 21550 frames Cell Events: 1088 mean_circ: 0.93343
Ran for 22050 frames Cell Events: 1113 mean_circ: 0.933081
Ran for 24050 frames Cell Events: 1213 mean_circ: 0.933372
Ran for 25050 frames Cell Events: 1258 mean_circ: 0.933474
Ran for 27050 frames Cell Events: 1350 mean_circ: 0.933868
Ran for 27550 frames Cell Events: 1374 mean_circ: 0.93375
Ran for 28050 frames Cell Events: 1395 mean_circ: 0.933901
Ran for 28550 frames Cell Events: 1430 mean_circ: 0.934027
Ran for 29050 frames Cell Events: 1453 mean_circ: 0.934256
Ran for 29550 frames Cell Events: 1474 mean_circ: 0.934074
Ran for 30050 frames Cell Events: 1491 mean_circ: 0.934086
Ran for 30550 frames Cell Events: 1513 mean_circ: 0.933922
Ran for 31050 frames Cell Events: 1541 mean_circ: 0.933722
Ran for 32550 frames Cell Events: 1605 mean_circ: 0.933235
Ran for 33050 frames Cell Events: 1620 mean_circ: 0.933362
Ran for 34050 frames Cell Events: 1659 mean_circ: 0.933479
Ran for 34550 frames Cell Events: 1685 mean_circ: 0.933657
Ran for 35050 frames Cell Events: 1715 mean_circ: 0.933804
Ran for 35550 frames Cell Events: 1746 mean_circ: 0.933986
Ran for 36050 frames Cell Events: 1772 mean_circ: 0.934225
Ran for 37050 frames Cell Events: 1810 mean_circ: 0.934153
Ran for 37550 frames Cell Events: 1835 mean_circ: 0.934229
Ran for 38050 frames Cell Events: 1852 mean_circ: 0.934137
Ran for 40050 frames Cell Events: 1955 mean_circ: 0.934389
Ran for 42550 frames Cell Events: 2077 mean_circ: 0.93414
Ran for 46050 frames Cell Events: 2256 mean_circ: 0.933995
Ran for 46550 frames Cell Events: 2279 mean_circ: 0.934115
Ran for 47050 frames Cell Events: 2304 mean_circ: 0.934382
Ran for 48050 frames Cell Events: 2355 mean_circ: 0.934501
Ran for 48550 frames Cell Events: 2384 mean_circ: 0.93434
Ran for 50050 frames Cell Events: 2466 mean_circ: 0.934214
Ran for 53050 frames Cell Events: 2611 mean_circ: 0.934337
Ran for 54550 frames Cell Events: 2717 mean_circ: 0.93434
Ran for 55050 frames Cell Events: 2746 mean_circ: 0.934394
Ran for 57050 frames Cell Events: 2851 mean_circ: 0.934514
Ran for 57550 frames Cell Events: 2872 mean_circ: 0.934499
Ran for 58050 frames Cell Events: 2891 mean_circ: 0.934418
Ran for 59050 frames Cell Events: 2935 mean_circ: 0.934668
Ran for 60050 frames Cell Events: 2976 mean_circ: 0.934539
Ran for 60550 frames Cell Events: 2999 mean_circ: 0.93458
Ran for 61050 frames Cell Events: 3019 mean_circ: 0.934655
Ran for 62050 frames Cell Events: 3074 mean_circ: 0.934591
Ran for 63050 frames Cell Events: 3115 mean_circ: 0.93471
Ran for 65550 frames Cell Events: 3269 mean_circ: 0.934697
Ran for 66550 frames Cell Events: 3319 mean_circ: 0.934567
Ran for 67050 frames Cell Events: 3339 mean_circ: 0.934425
Ran for 68050 frames Cell Events: 3371 mean_circ: 0.934496
Ran for 69550 frames Cell Events: 3443 mean_circ: 0.934403
Ran for 70050 frames Cell Events: 3477 mean_circ: 0.934511
Ran for 70550 frames Cell Events: 3499 mean_circ: 0.934549
Ran for 71550 frames Cell Events: 3550 mean_circ: 0.934562
Ran for 73550 frames Cell Events: 3648 mean_circ: 0.934584
Ran for 75050 frames Cell Events: 3737 mean_circ: 0.9346
Ran for 75550 frames Cell Events: 3768 mean_circ: 0.934502
Ran for 76050 frames Cell Events: 3793 mean_circ: 0.934515
Ran for 76550 frames Cell Events: 3816 mean_circ: 0.934475
Ran for 78550 frames Cell Events: 3911 mean_circ: 0.934526
Ran for 79050 frames Cell Events: 3949 mean_circ: 0.934494
Ran for 79550 frames Cell Events: 3972 mean_circ: 0.934533
Ran for 80050 frames Cell Events: 3996 mean_circ: 0.93442
Ran for 80550 frames Cell Events: 4022 mean_circ: 0.934398
Ran for 81550 frames Cell Events: 4085 mean_circ: 0.934413
Ran for 82050 frames Cell Events: 4108 mean_circ: 0.934465
Ran for 82550 frames Cell Events: 4122 mean_circ: 0.934432
Ran for 84050 frames Cell Events: 4182 mean_circ: 0.93444
Ran for 84550 frames Cell Events: 4218 mean_circ: 0.934219
Ran for 87050 frames Cell Events: 4382 mean_circ: 0.934289
Ran for 87550 frames Cell Events: 4414 mean_circ: 0.934284
Ran for 88550 frames Cell Events: 4456 mean_circ: 0.934305
Ran for 89050 frames Cell Events: 4481 mean_circ: 0.934305
Ran for 89550 frames Cell Events: 4494 mean_circ: 0.934416
Ran for 90050 frames Cell Events: 4514 mean_circ: 0.934452
Ran for 90550 frames Cell Events: 4536 mean_circ: 0.934421
Ran for 92050 frames Cell Events: 4620 mean_circ: 0.934335
Ran for 92550 frames Cell Events: 4637 mean_circ: 0.934343
Ran for 93550 frames Cell Events: 4696 mean_circ: 0.934371
Ran for 96050 frames Cell Events: 4823 mean_circ: 0.934353
Ran for 96550 frames Cell Events: 4849 mean_circ: 0.934306
Ran for 97050 frames Cell Events: 4864 mean_circ: 0.934297
Ran for 97550 frames Cell Events: 4886 mean_circ: 0.934332
Ran for 98050 frames Cell Events: 4914 mean_circ: 0.934362
Ran for 99050 frames Cell Events: 4957 mean_circ: 0.934313
Ran for 100550 frames Cell Events: 5049 mean_circ: 0.93438
Ran for 101050 frames Cell Events: 5068 mean_circ: 0.934334
Ran for 102050 frames Cell Events: 5116 mean_circ: 0.934214
Ran for 103050 frames Cell Events: 5176 mean_circ: 0.934279
Ran for 104050 frames Cell Events: 5237 mean_circ: 0.934315
Ran for 104550 frames Cell Events: 5258 mean_circ: 0.934245
Ran for 105550 frames Cell Events: 5302 mean_circ: 0.934201
Ran for 108550 frames Cell Events: 5511 mean_circ: 0.934266
Ran for 109050 frames Cell Events: 5539 mean_circ: 0.934236
Ran for 110050 frames Cell Events: 5594 mean_circ: 0.934173
Ran for 110550 frames Cell Events: 5620 mean_circ: 0.9342
Ran for 112550 frames Cell Events: 5752 mean_circ: 0.934132
Ran for 113550 frames Cell Events: 5801 mean_circ: 0.934194
Ran for 114050 frames Cell Events: 5826 mean_circ: 0.934235
Ran for 115050 frames Cell Events: 5858 mean_circ: 0.934248
Ran for 116050 frames Cell Events: 5920 mean_circ: 0.93421
Ran for 117550 frames Cell Events: 6006 mean_circ: 0.934253
Ran for 118050 frames Cell Events: 6031 mean_circ: 0.934253
Ran for 118550 frames Cell Events: 6051 mean_circ: 0.934229
Ran for 119550 frames Cell Events: 6119 mean_circ: 0.934278
Ran for 120050 frames Cell Events: 6139 mean_circ: 0.93426
Ran for 122050 frames Cell Events: 6254 mean_circ: 0.934254
Ran for 122550 frames Cell Events: 6269 mean_circ: 0.934246
Ran for 123550 frames Cell Events: 6327 mean_circ: 0.934222
Ran for 125050 frames Cell Events: 6396 mean_circ: 0.934265
Ran for 125550 frames Cell Events: 6425 mean_circ: 0.934333
Ran for 126050 frames Cell Events: 6465 mean_circ: 0.934333
Ran for 127550 frames Cell Events: 6568 mean_circ: 0.934179
Ran for 128550 frames Cell Events: 6645 mean_circ: 0.934205
Ran for 129550 frames Cell Events: 6706 mean_circ: 0.934281
Ran for 130050 frames Cell Events: 6741 mean_circ: 0.9343
Ran for 130550 frames Cell Events: 6770 mean_circ: 0.934286
Ran for 131550 frames Cell Events: 6820 mean_circ: 0.934289
Ran for 132550 frames Cell Events: 6870 mean_circ: 0.934331
Ran for 133050 frames Cell Events: 6895 mean_circ: 0.934331
Ran for 134050 frames Cell Events: 6946 mean_circ: 0.934291
Ran for 135050 frames Cell Events: 6999 mean_circ: 0.934292
Ran for 136050 frames Cell Events: 7059 mean_circ: 0.934259
Ran for 136550 frames Cell Events: 7083 mean_circ: 0.93425
Ran for 137050 frames Cell Events: 7103 mean_circ: 0.934263
Ran for 137550 frames Cell Events: 7128 mean_circ: 0.934282
Ran for 138050 frames Cell Events: 7153 mean_circ: 0.934292
Ran for 138550 frames Cell Events: 7174 mean_circ: 0.934314
Ran for 139050 frames Cell Events: 7199 mean_circ: 0.934336
Ran for 141050 frames Cell Events: 7310 mean_circ: 0.93436
Ran for 142550 frames Cell Events: 7380 mean_circ: 0.934269
Ran for 143550 frames Cell Events: 7410 mean_circ: 0.934266
Ran for 144050 frames Cell Events: 7426 mean_circ: 0.934287
Ran for 146050 frames Cell Events: 7534 mean_circ: 0.934174
Ran for 146550 frames Cell Events: 7554 mean_circ: 0.934188
Ran for 147050 frames Cell Events: 7567 mean_circ: 0.934194
Ran for 147550 frames Cell Events: 7589 mean_circ: 0.934164
Ran for 149050 frames Cell Events: 7660 mean_circ: 0.934149
Ran for 150550 frames Cell Events: 7718 mean_circ: 0.934086
Ran for 151050 frames Cell Events: 7745 mean_circ: 0.934109
Ran for 151550 frames Cell Events: 7783 mean_circ: 0.934138
Ran for 152050 frames Cell Events: 7811 mean_circ: 0.934052
Ran for 153050 frames Cell Events: 7859 mean_circ: 0.93403
Ran for 153550 frames Cell Events: 7887 mean_circ: 0.934031
Ran for 154550 frames Cell Events: 7932 mean_circ: 0.934001
Ran for 155050 frames Cell Events: 7955 mean_circ: 0.934024
Ran for 155550 frames Cell Events: 7980 mean_circ: 0.934015
Ran for 156050 frames Cell Events: 8009 mean_circ: 0.934011
Ran for 156550 frames Cell Events: 8033 mean_circ: 0.934027
Ran for 157050 frames Cell Events: 8068 mean_circ: 0.934056
End this measurment..
Stopping time: 7.15425e+008
****************************************
Measurement finished
Mean Circularitiy: 0.934051
Mean Area: 634.994
Mean FL-1 intensity: 3382.08
Mean FL-2 intensity: 4856.68
Mean FL-3 intensity: 0
frames processed: 156387
Total Time: 78810ms
Number of Runs (new bg img) 0
Frames collected: 157493
Skipped frames: 35707
Rescued Frames: 1558662
Samples DAQ: 39373981
Samples written: 78746280
Samples per Channel: 39373140
FPS: 1998.39
Samples per second: 499596
Expected samples/s: 500000
Events: 8091
Events per second: 102.665
jumped back (PMT): 25
#Frames read, frequency
# 0 frames :240016
# 1 frames :62195
# 2 frames :3180
# 3 frames :1138
# 4 frames :677
# 5 frames :283
# 6 frames :1205
# 7 frames :1352
# 8 frames :924
# 9 frames :634
# 10 frames :615
# 11 frames :637
# 12 frames :413
# 13 frames :212
# 14 frames :189
# 15 frames :193
# 16 frames :157
# 17 frames :145
# 18 frames :123
# 19 frames :97
# 20 frames :65
# 21 frames :75
# 22 frames :46
# 23 frames :48
# 24 frames :45
# 25 frames :31
# 26 frames :34
# 27 frames :29
# 28 frames :33
# 29 frames :22
# 30 frames :16
# 31 frames :16
# 32 frames :7
# 33 frames :9
# 34 frames :10
# 35 frames :9
# 36 frames :11
# 37 frames :10
# 38 frames :8
# 39 frames :1
# 40 frames :8
# 41 frames :2
# 42 frames :6
# 43 frames :1
# 44 frames :3
# 45 frames :4
# 46 frames :3
# 47 frames :5
# 48 frames :3
# 49 frames :3
# 50 frames :1
# 51 frames :3
# 52 frames :1
# 54 frames :1
# 55 frames :5
# 58 frames :4
# 59 frames :1
# 66 frames :2
# 74 frames :1
# 75 frames :1
M1_para.ini
[General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100
[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0
#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 25.0972
parameters.txt
#fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0