Click on the log titles below to expand the log entries present in this DC resource.
dclab-export_2024-07-09_19.59.38
{"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc
{
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "589167cda422b28aa6a5304048e9de41",
"name": "M1_20170523_1616_dsRNA_F5_faster.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-09",
"time": "17:59:58"
}
}
dclab-tdms2rtdc-warnings
LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8049 (max 8050) in '8049'.
FG_Config.mcf
[Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;
[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;
[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini
[Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6
[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini
[EVENT LOG] fRT-DC V1.06170206
Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 25.1068
Start pulse train for BG Aquisition..
Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 7.19645e+008
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 550 frames Cell Events: 20 mean_circ: 0.926135
Ran for 1050 frames Cell Events: 37 mean_circ: 0.931782
Ran for 3050 frames Cell Events: 152 mean_circ: 0.926905
Ran for 3550 frames Cell Events: 178 mean_circ: 0.928479
Ran for 4550 frames Cell Events: 196 mean_circ: 0.930716
Ran for 5050 frames Cell Events: 216 mean_circ: 0.931207
Ran for 6550 frames Cell Events: 305 mean_circ: 0.933079
Ran for 7050 frames Cell Events: 330 mean_circ: 0.931771
Ran for 7550 frames Cell Events: 355 mean_circ: 0.931532
Ran for 8050 frames Cell Events: 378 mean_circ: 0.932279
Ran for 9050 frames Cell Events: 439 mean_circ: 0.932963
Ran for 10050 frames Cell Events: 508 mean_circ: 0.932993
Ran for 11050 frames Cell Events: 562 mean_circ: 0.933653
Ran for 11550 frames Cell Events: 584 mean_circ: 0.933315
Ran for 12550 frames Cell Events: 638 mean_circ: 0.933489
Ran for 13050 frames Cell Events: 665 mean_circ: 0.932955
Ran for 13550 frames Cell Events: 693 mean_circ: 0.93302
Ran for 14550 frames Cell Events: 744 mean_circ: 0.931736
Ran for 15050 frames Cell Events: 768 mean_circ: 0.931733
Ran for 16050 frames Cell Events: 808 mean_circ: 0.932139
Ran for 17050 frames Cell Events: 874 mean_circ: 0.932061
Ran for 17550 frames Cell Events: 900 mean_circ: 0.931865
Ran for 18050 frames Cell Events: 915 mean_circ: 0.93204
Ran for 18550 frames Cell Events: 931 mean_circ: 0.932324
Ran for 19050 frames Cell Events: 951 mean_circ: 0.932345
Ran for 20050 frames Cell Events: 997 mean_circ: 0.932832
Ran for 20550 frames Cell Events: 1017 mean_circ: 0.932992
Ran for 21550 frames Cell Events: 1077 mean_circ: 0.932625
Ran for 22550 frames Cell Events: 1136 mean_circ: 0.932814
Ran for 23050 frames Cell Events: 1169 mean_circ: 0.9328
Ran for 23550 frames Cell Events: 1192 mean_circ: 0.93304
Ran for 24050 frames Cell Events: 1217 mean_circ: 0.932945
Ran for 25050 frames Cell Events: 1246 mean_circ: 0.933096
Ran for 26550 frames Cell Events: 1325 mean_circ: 0.932957
Ran for 27550 frames Cell Events: 1374 mean_circ: 0.932759
Ran for 28550 frames Cell Events: 1407 mean_circ: 0.932881
Ran for 29050 frames Cell Events: 1422 mean_circ: 0.932532
Ran for 29550 frames Cell Events: 1453 mean_circ: 0.932597
Ran for 30050 frames Cell Events: 1472 mean_circ: 0.93276
Ran for 31050 frames Cell Events: 1523 mean_circ: 0.932526
Ran for 31550 frames Cell Events: 1537 mean_circ: 0.932613
Ran for 33050 frames Cell Events: 1605 mean_circ: 0.932334
Ran for 34550 frames Cell Events: 1663 mean_circ: 0.932236
Ran for 35050 frames Cell Events: 1691 mean_circ: 0.932311
Ran for 36050 frames Cell Events: 1746 mean_circ: 0.932467
Ran for 37050 frames Cell Events: 1810 mean_circ: 0.932659
Ran for 38050 frames Cell Events: 1868 mean_circ: 0.933038
Ran for 39050 frames Cell Events: 1935 mean_circ: 0.932511
Ran for 40050 frames Cell Events: 1978 mean_circ: 0.932467
Ran for 41050 frames Cell Events: 2029 mean_circ: 0.932632
Ran for 41550 frames Cell Events: 2045 mean_circ: 0.932531
Ran for 42050 frames Cell Events: 2055 mean_circ: 0.932696
Ran for 43050 frames Cell Events: 2103 mean_circ: 0.932895
Ran for 44050 frames Cell Events: 2158 mean_circ: 0.933082
Ran for 44550 frames Cell Events: 2170 mean_circ: 0.933089
Ran for 45050 frames Cell Events: 2190 mean_circ: 0.932979
Ran for 46050 frames Cell Events: 2251 mean_circ: 0.933036
Ran for 46550 frames Cell Events: 2274 mean_circ: 0.933063
Ran for 47050 frames Cell Events: 2307 mean_circ: 0.933218
Ran for 47550 frames Cell Events: 2332 mean_circ: 0.933435
Ran for 48050 frames Cell Events: 2366 mean_circ: 0.933465
Ran for 48550 frames Cell Events: 2402 mean_circ: 0.933418
Ran for 49050 frames Cell Events: 2430 mean_circ: 0.933509
Ran for 49550 frames Cell Events: 2452 mean_circ: 0.933564
Ran for 50550 frames Cell Events: 2509 mean_circ: 0.933433
Ran for 51550 frames Cell Events: 2554 mean_circ: 0.933452
Ran for 52050 frames Cell Events: 2585 mean_circ: 0.933441
Ran for 52550 frames Cell Events: 2604 mean_circ: 0.933567
Ran for 53050 frames Cell Events: 2624 mean_circ: 0.933591
Ran for 53550 frames Cell Events: 2656 mean_circ: 0.933647
Ran for 54050 frames Cell Events: 2671 mean_circ: 0.933721
Ran for 54550 frames Cell Events: 2685 mean_circ: 0.933736
Ran for 55550 frames Cell Events: 2750 mean_circ: 0.933547
Ran for 56050 frames Cell Events: 2773 mean_circ: 0.933574
Ran for 57050 frames Cell Events: 2824 mean_circ: 0.933559
Ran for 58050 frames Cell Events: 2867 mean_circ: 0.933505
Ran for 58550 frames Cell Events: 2896 mean_circ: 0.933422
Ran for 59550 frames Cell Events: 2946 mean_circ: 0.933461
Ran for 60050 frames Cell Events: 2968 mean_circ: 0.933574
Ran for 62550 frames Cell Events: 3104 mean_circ: 0.93376
Ran for 63550 frames Cell Events: 3157 mean_circ: 0.933814
Ran for 64050 frames Cell Events: 3189 mean_circ: 0.933873
Ran for 65050 frames Cell Events: 3238 mean_circ: 0.933828
Ran for 65550 frames Cell Events: 3268 mean_circ: 0.933668
Ran for 67050 frames Cell Events: 3353 mean_circ: 0.933909
Ran for 67550 frames Cell Events: 3365 mean_circ: 0.933915
Ran for 68050 frames Cell Events: 3388 mean_circ: 0.933804
Ran for 69050 frames Cell Events: 3443 mean_circ: 0.933748
Ran for 69550 frames Cell Events: 3470 mean_circ: 0.933753
Ran for 71050 frames Cell Events: 3548 mean_circ: 0.933722
Ran for 72550 frames Cell Events: 3627 mean_circ: 0.933738
Ran for 73050 frames Cell Events: 3648 mean_circ: 0.933758
Ran for 74550 frames Cell Events: 3732 mean_circ: 0.933731
Ran for 76050 frames Cell Events: 3808 mean_circ: 0.933585
Ran for 77050 frames Cell Events: 3862 mean_circ: 0.933656
Ran for 77550 frames Cell Events: 3882 mean_circ: 0.933702
Ran for 79050 frames Cell Events: 3960 mean_circ: 0.933717
Ran for 80050 frames Cell Events: 4020 mean_circ: 0.93371
Ran for 80550 frames Cell Events: 4055 mean_circ: 0.933676
Ran for 83050 frames Cell Events: 4190 mean_circ: 0.933595
Ran for 83550 frames Cell Events: 4213 mean_circ: 0.933586
Ran for 84550 frames Cell Events: 4276 mean_circ: 0.933584
Ran for 85050 frames Cell Events: 4295 mean_circ: 0.933564
Ran for 85550 frames Cell Events: 4323 mean_circ: 0.933556
Ran for 86550 frames Cell Events: 4368 mean_circ: 0.933641
Ran for 87050 frames Cell Events: 4396 mean_circ: 0.933634
Ran for 88550 frames Cell Events: 4490 mean_circ: 0.93369
Ran for 89050 frames Cell Events: 4517 mean_circ: 0.933703
Ran for 90550 frames Cell Events: 4567 mean_circ: 0.933516
Ran for 91050 frames Cell Events: 4589 mean_circ: 0.933507
Ran for 91550 frames Cell Events: 4616 mean_circ: 0.933523
Ran for 92050 frames Cell Events: 4638 mean_circ: 0.93341
Ran for 93050 frames Cell Events: 4686 mean_circ: 0.933332
Ran for 94050 frames Cell Events: 4731 mean_circ: 0.933122
Ran for 94550 frames Cell Events: 4768 mean_circ: 0.933122
Ran for 95050 frames Cell Events: 4785 mean_circ: 0.933032
Ran for 96050 frames Cell Events: 4831 mean_circ: 0.932988
Ran for 96550 frames Cell Events: 4863 mean_circ: 0.932993
Ran for 98550 frames Cell Events: 4974 mean_circ: 0.932979
Ran for 99050 frames Cell Events: 5001 mean_circ: 0.933024
Ran for 99550 frames Cell Events: 5021 mean_circ: 0.933047
Ran for 102550 frames Cell Events: 5178 mean_circ: 0.933085
Ran for 103550 frames Cell Events: 5230 mean_circ: 0.933182
Ran for 104050 frames Cell Events: 5259 mean_circ: 0.933182
Ran for 104550 frames Cell Events: 5292 mean_circ: 0.933211
Ran for 106050 frames Cell Events: 5373 mean_circ: 0.933392
Ran for 107050 frames Cell Events: 5423 mean_circ: 0.933343
Ran for 107550 frames Cell Events: 5444 mean_circ: 0.933387
Ran for 109050 frames Cell Events: 5506 mean_circ: 0.933362
Ran for 110550 frames Cell Events: 5584 mean_circ: 0.933411
Ran for 111050 frames Cell Events: 5601 mean_circ: 0.933447
Ran for 111550 frames Cell Events: 5624 mean_circ: 0.933402
Ran for 112550 frames Cell Events: 5682 mean_circ: 0.933342
Ran for 113550 frames Cell Events: 5742 mean_circ: 0.933324
Ran for 114550 frames Cell Events: 5782 mean_circ: 0.933394
Ran for 116550 frames Cell Events: 5896 mean_circ: 0.933385
Ran for 117050 frames Cell Events: 5913 mean_circ: 0.933401
Ran for 118550 frames Cell Events: 5969 mean_circ: 0.933372
Ran for 120050 frames Cell Events: 6036 mean_circ: 0.933321
Ran for 120550 frames Cell Events: 6062 mean_circ: 0.933333
Ran for 121050 frames Cell Events: 6082 mean_circ: 0.933261
Ran for 121550 frames Cell Events: 6093 mean_circ: 0.933266
Ran for 122550 frames Cell Events: 6150 mean_circ: 0.933365
Ran for 125050 frames Cell Events: 6268 mean_circ: 0.933484
Ran for 125550 frames Cell Events: 6294 mean_circ: 0.933512
Ran for 126550 frames Cell Events: 6370 mean_circ: 0.933569
Ran for 127050 frames Cell Events: 6400 mean_circ: 0.933537
Ran for 129050 frames Cell Events: 6493 mean_circ: 0.93341
Ran for 129550 frames Cell Events: 6511 mean_circ: 0.933474
Ran for 130050 frames Cell Events: 6528 mean_circ: 0.933492
Ran for 132050 frames Cell Events: 6618 mean_circ: 0.933523
Ran for 133050 frames Cell Events: 6652 mean_circ: 0.933505
Ran for 137550 frames Cell Events: 6899 mean_circ: 0.933588
Ran for 138050 frames Cell Events: 6918 mean_circ: 0.93357
Ran for 139050 frames Cell Events: 6976 mean_circ: 0.933507
Ran for 139550 frames Cell Events: 6994 mean_circ: 0.933483
Ran for 141050 frames Cell Events: 7075 mean_circ: 0.933447
Ran for 143550 frames Cell Events: 7238 mean_circ: 0.933709
Ran for 145550 frames Cell Events: 7350 mean_circ: 0.933674
Ran for 146550 frames Cell Events: 7405 mean_circ: 0.93372
Ran for 149050 frames Cell Events: 7555 mean_circ: 0.933707
Ran for 149550 frames Cell Events: 7587 mean_circ: 0.933744
Ran for 150550 frames Cell Events: 7645 mean_circ: 0.933744
Ran for 151550 frames Cell Events: 7691 mean_circ: 0.933765
Ran for 152050 frames Cell Events: 7717 mean_circ: 0.933768
Ran for 152550 frames Cell Events: 7746 mean_circ: 0.933687
Ran for 153050 frames Cell Events: 7771 mean_circ: 0.933704
Ran for 153550 frames Cell Events: 7802 mean_circ: 0.933729
Ran for 157050 frames Cell Events: 8002 mean_circ: 0.933882
Ran for 157550 frames Cell Events: 8038 mean_circ: 0.933864
End this measurment..
Stopping time: 7.19724e+008
****************************************
Measurement finished
Mean Circularitiy: 0.933871
Mean Area: 630.173
Mean FL-1 intensity: 3215.8
Mean FL-2 intensity: 4277.71
Mean FL-3 intensity: 0
frames processed: 155972
Total Time: 78989ms
Number of Runs (new bg img) 0
Frames collected: 157831
Skipped frames: 70560
Rescued Frames: 2510139
Samples DAQ: 39457725
Samples written: 78915318
Samples per Channel: 39457659
FPS: 1998.14
Samples per second: 499534
Expected samples/s: 500000
Events: 8050
Events per second: 101.913
jumped back (PMT): 17
#Frames read, frequency
# 0 frames :238865
# 1 frames :64109
# 2 frames :3031
# 3 frames :1060
# 4 frames :577
# 5 frames :301
# 6 frames :2084
# 7 frames :1190
# 8 frames :1012
# 9 frames :596
# 10 frames :471
# 11 frames :377
# 12 frames :272
# 13 frames :216
# 14 frames :217
# 15 frames :169
# 16 frames :145
# 17 frames :114
# 18 frames :93
# 19 frames :100
# 20 frames :97
# 21 frames :85
# 22 frames :61
# 23 frames :57
# 24 frames :35
# 25 frames :36
# 26 frames :28
# 27 frames :28
# 28 frames :25
# 29 frames :27
# 30 frames :26
# 31 frames :16
# 32 frames :13
# 33 frames :8
# 34 frames :5
# 35 frames :8
# 36 frames :9
# 37 frames :8
# 38 frames :10
# 39 frames :7
# 40 frames :1
# 41 frames :5
# 42 frames :8
# 43 frames :3
# 44 frames :2
# 45 frames :2
# 46 frames :2
# 47 frames :2
# 48 frames :2
# 49 frames :4
# 50 frames :1
# 52 frames :1
# 54 frames :2
# 55 frames :1
# 56 frames :2
# 57 frames :1
# 58 frames :1
# 59 frames :2
# 62 frames :1
# 64 frames :1
# 66 frames :1
# 74 frames :1
M1_para.ini
[General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100
[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0
#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 25.1068
parameters.txt
#fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0