Click on the log titles below to expand the log entries present in this DC resource.
dclab-export_2024-07-09_20.10.03
{"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc
{
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "c3fd119d1a0c6cf2af2e060ab5732363",
"name": "M1_20170523_1905_dsRNA_H6_faster.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-09",
"time": "18:10:23"
}
}
FG_Config.mcf
[Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;
[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;
[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini
[Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6
[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini
[EVENT LOG] fRT-DC V1.06170206
Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 25.1422
Start pulse train for BG Aquisition..
Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 7.2977e+008
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 50 frames Cell Events: 0 mean_circ: 0.950118
Ran for 550 frames Cell Events: 16 mean_circ: 0.949033
Ran for 1550 frames Cell Events: 46 mean_circ: 0.943772
Ran for 2050 frames Cell Events: 56 mean_circ: 0.93929
Ran for 4550 frames Cell Events: 132 mean_circ: 0.942083
Ran for 5050 frames Cell Events: 147 mean_circ: 0.941831
Ran for 5550 frames Cell Events: 177 mean_circ: 0.939247
Ran for 6050 frames Cell Events: 197 mean_circ: 0.939468
Ran for 7050 frames Cell Events: 232 mean_circ: 0.939678
Ran for 8050 frames Cell Events: 281 mean_circ: 0.94044
Ran for 8550 frames Cell Events: 300 mean_circ: 0.940439
Ran for 10050 frames Cell Events: 356 mean_circ: 0.942338
Ran for 10550 frames Cell Events: 383 mean_circ: 0.941652
Ran for 11050 frames Cell Events: 397 mean_circ: 0.941919
Ran for 11550 frames Cell Events: 419 mean_circ: 0.941696
Ran for 12550 frames Cell Events: 442 mean_circ: 0.941105
Ran for 13050 frames Cell Events: 456 mean_circ: 0.941449
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Ran for 16050 frames Cell Events: 541 mean_circ: 0.94205
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Ran for 30050 frames Cell Events: 1080 mean_circ: 0.942165
Ran for 30550 frames Cell Events: 1098 mean_circ: 0.942286
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Ran for 44050 frames Cell Events: 1631 mean_circ: 0.941107
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Ran for 63550 frames Cell Events: 2381 mean_circ: 0.940698
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Ran for 64550 frames Cell Events: 2419 mean_circ: 0.940812
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Ran for 73550 frames Cell Events: 2819 mean_circ: 0.940776
Ran for 74050 frames Cell Events: 2835 mean_circ: 0.940682
Ran for 74550 frames Cell Events: 2862 mean_circ: 0.940755
Ran for 75050 frames Cell Events: 2880 mean_circ: 0.940755
Ran for 75550 frames Cell Events: 2893 mean_circ: 0.940699
Ran for 76550 frames Cell Events: 2920 mean_circ: 0.940753
Ran for 77050 frames Cell Events: 2942 mean_circ: 0.940843
Ran for 79050 frames Cell Events: 3022 mean_circ: 0.940814
Ran for 80550 frames Cell Events: 3113 mean_circ: 0.940746
Ran for 81050 frames Cell Events: 3147 mean_circ: 0.940759
Ran for 81550 frames Cell Events: 3165 mean_circ: 0.940759
Ran for 83050 frames Cell Events: 3226 mean_circ: 0.940792
Ran for 83550 frames Cell Events: 3240 mean_circ: 0.940781
Ran for 84050 frames Cell Events: 3262 mean_circ: 0.940772
Ran for 84550 frames Cell Events: 3284 mean_circ: 0.94072
Ran for 85050 frames Cell Events: 3319 mean_circ: 0.940822
Ran for 85550 frames Cell Events: 3334 mean_circ: 0.940887
Ran for 86550 frames Cell Events: 3379 mean_circ: 0.940749
Ran for 87550 frames Cell Events: 3423 mean_circ: 0.940843
Ran for 88050 frames Cell Events: 3435 mean_circ: 0.940898
Ran for 88550 frames Cell Events: 3453 mean_circ: 0.940828
Ran for 90050 frames Cell Events: 3535 mean_circ: 0.940723
Ran for 92050 frames Cell Events: 3614 mean_circ: 0.940679
Ran for 92550 frames Cell Events: 3629 mean_circ: 0.940675
Ran for 94050 frames Cell Events: 3684 mean_circ: 0.940634
Ran for 94550 frames Cell Events: 3699 mean_circ: 0.940598
Ran for 95050 frames Cell Events: 3721 mean_circ: 0.940618
Ran for 95550 frames Cell Events: 3732 mean_circ: 0.940668
Ran for 96550 frames Cell Events: 3764 mean_circ: 0.940637
Ran for 97550 frames Cell Events: 3809 mean_circ: 0.940461
Ran for 99550 frames Cell Events: 3883 mean_circ: 0.940392
Ran for 100550 frames Cell Events: 3912 mean_circ: 0.940483
Ran for 101050 frames Cell Events: 3925 mean_circ: 0.940434
Ran for 101550 frames Cell Events: 3941 mean_circ: 0.940442
Ran for 102050 frames Cell Events: 3970 mean_circ: 0.940492
Ran for 102550 frames Cell Events: 3986 mean_circ: 0.940472
Ran for 103050 frames Cell Events: 4007 mean_circ: 0.940435
Ran for 103550 frames Cell Events: 4020 mean_circ: 0.940478
Ran for 104050 frames Cell Events: 4035 mean_circ: 0.940489
Ran for 105050 frames Cell Events: 4068 mean_circ: 0.940443
Ran for 106050 frames Cell Events: 4110 mean_circ: 0.940378
Ran for 106550 frames Cell Events: 4128 mean_circ: 0.940388
Ran for 107550 frames Cell Events: 4173 mean_circ: 0.940353
Ran for 108050 frames Cell Events: 4188 mean_circ: 0.940378
Ran for 108550 frames Cell Events: 4207 mean_circ: 0.94034
Ran for 109050 frames Cell Events: 4230 mean_circ: 0.940316
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Ran for 116050 frames Cell Events: 4506 mean_circ: 0.940399
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Ran for 120050 frames Cell Events: 4686 mean_circ: 0.940556
Ran for 120550 frames Cell Events: 4704 mean_circ: 0.940559
Ran for 122050 frames Cell Events: 4744 mean_circ: 0.940543
Ran for 122550 frames Cell Events: 4756 mean_circ: 0.940538
Ran for 123050 frames Cell Events: 4772 mean_circ: 0.940528
Ran for 123550 frames Cell Events: 4799 mean_circ: 0.9406
Ran for 125050 frames Cell Events: 4866 mean_circ: 0.940461
Ran for 125550 frames Cell Events: 4884 mean_circ: 0.940488
Ran for 127050 frames Cell Events: 4945 mean_circ: 0.940448
Ran for 127550 frames Cell Events: 4957 mean_circ: 0.940439
Ran for 128550 frames Cell Events: 4984 mean_circ: 0.940427
Ran for 129050 frames Cell Events: 4996 mean_circ: 0.94044
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Ran for 130550 frames Cell Events: 5054 mean_circ: 0.940391
Ran for 131550 frames Cell Events: 5113 mean_circ: 0.940309
Ran for 132550 frames Cell Events: 5148 mean_circ: 0.940304
Ran for 133050 frames Cell Events: 5164 mean_circ: 0.940318
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Ran for 135550 frames Cell Events: 5235 mean_circ: 0.940196
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Ran for 138050 frames Cell Events: 5325 mean_circ: 0.940307
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Ran for 139550 frames Cell Events: 5381 mean_circ: 0.940322
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Ran for 140550 frames Cell Events: 5418 mean_circ: 0.940406
Ran for 143050 frames Cell Events: 5511 mean_circ: 0.940294
Ran for 143550 frames Cell Events: 5522 mean_circ: 0.9403
Ran for 144550 frames Cell Events: 5559 mean_circ: 0.940251
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Ran for 146050 frames Cell Events: 5612 mean_circ: 0.940258
Ran for 147050 frames Cell Events: 5644 mean_circ: 0.940242
Ran for 147550 frames Cell Events: 5665 mean_circ: 0.94021
Ran for 148050 frames Cell Events: 5681 mean_circ: 0.940213
Ran for 148550 frames Cell Events: 5698 mean_circ: 0.940252
Ran for 149050 frames Cell Events: 5711 mean_circ: 0.940245
Ran for 151050 frames Cell Events: 5812 mean_circ: 0.940154
Ran for 151550 frames Cell Events: 5828 mean_circ: 0.940104
Ran for 152050 frames Cell Events: 5851 mean_circ: 0.940073
Ran for 153050 frames Cell Events: 5888 mean_circ: 0.940049
Ran for 153550 frames Cell Events: 5903 mean_circ: 0.940057
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Ran for 158050 frames Cell Events: 6071 mean_circ: 0.940136
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Ran for 196050 frames Cell Events: 7476 mean_circ: 0.940226
Ran for 196550 frames Cell Events: 7492 mean_circ: 0.940204
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Ran for 198050 frames Cell Events: 7544 mean_circ: 0.940089
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Ran for 199050 frames Cell Events: 7571 mean_circ: 0.940042
Ran for 199550 frames Cell Events: 7582 mean_circ: 0.940048
Ran for 200550 frames Cell Events: 7623 mean_circ: 0.940054
Ran for 201050 frames Cell Events: 7653 mean_circ: 0.940089
Ran for 201550 frames Cell Events: 7674 mean_circ: 0.940095
Ran for 202550 frames Cell Events: 7707 mean_circ: 0.940097
Ran for 203050 frames Cell Events: 7721 mean_circ: 0.940105
Ran for 205050 frames Cell Events: 7802 mean_circ: 0.940116
Ran for 206050 frames Cell Events: 7842 mean_circ: 0.940084
Ran for 207550 frames Cell Events: 7900 mean_circ: 0.940025
Ran for 208550 frames Cell Events: 7952 mean_circ: 0.939973
Ran for 209550 frames Cell Events: 8004 mean_circ: 0.939906
Ran for 210550 frames Cell Events: 8057 mean_circ: 0.939916
End this measurment..
Stopping time: 7.29876e+008
****************************************
Measurement finished
Mean Circularitiy: 0.939924
Mean Area: 592.529
Mean FL-1 intensity: 3556.51
Mean FL-2 intensity: 4677.64
Mean FL-3 intensity: 0
frames processed: 208374
Total Time: 105472ms
Number of Runs (new bg img) 0
Frames collected: 210814
Skipped frames: 136239
Rescued Frames: 4421063
Samples DAQ: 52703547
Samples written: 105407032
Samples per Channel: 52703516
FPS: 1998.76
Samples per second: 499690
Expected samples/s: 500000
Events: 8069
Events per second: 76.5034
jumped back (PMT): 14
#Frames read, frequency
# 0 frames :296236
# 1 frames :110242
# 2 frames :3941
# 3 frames :1409
# 4 frames :746
# 5 frames :347
# 6 frames :2494
# 7 frames :1489
# 8 frames :1207
# 9 frames :673
# 10 frames :621
# 11 frames :448
# 12 frames :344
# 13 frames :263
# 14 frames :230
# 15 frames :159
# 16 frames :140
# 17 frames :100
# 18 frames :82
# 19 frames :69
# 20 frames :56
# 21 frames :39
# 22 frames :47
# 23 frames :37
# 24 frames :29
# 25 frames :15
# 26 frames :18
# 27 frames :17
# 28 frames :10
# 29 frames :11
# 30 frames :9
# 31 frames :9
# 32 frames :8
# 33 frames :7
# 34 frames :2
# 35 frames :9
# 36 frames :3
# 37 frames :5
# 38 frames :5
# 39 frames :5
# 40 frames :3
# 42 frames :1
# 43 frames :2
# 44 frames :1
# 45 frames :2
# 46 frames :2
# 48 frames :1
# 49 frames :3
# 51 frames :1
# 52 frames :1
# 54 frames :1
# 56 frames :1
# 60 frames :1
# 85 frames :1
M1_para.ini
[General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100
[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0
#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 25.1422
parameters.txt
#fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0