M1_20170523_1946_dsRNA_H5_col.rtdc
Click on the log titles below to expand the log entries present in this DC resource.
dclab-export_2024-07-09_20.12.21
{"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}dclab-tdms2rtdc
{"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "ad2bb9289fb23b06cb7d718b712b95e6",
"name": "M1_20170523_1946_dsRNA_H5_col.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-09",
"time": "18:12:41"
}
}
dclab-tdms2rtdc-warnings
LimitingExportSizeWarningin dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8206 (max 8207) in '8206'.
FG_Config.mcf
[Kamerasektion]ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;
[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;
[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini
[Framerate]Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6
[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini
[EVENT LOG] fRT-DC V1.06170206Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 25.1914
Start pulse train for BG Aquisition..
Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 7.32235e+008
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 550 frames Cell Events: 6 mean_circ: 0.908216
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Ran for 770050 frames Cell Events: 7374 mean_circ: 0.926799
Ran for 770550 frames Cell Events: 7381 mean_circ: 0.926818
Ran for 771550 frames Cell Events: 7389 mean_circ: 0.926826
Ran for 772050 frames Cell Events: 7394 mean_circ: 0.926815
Ran for 772550 frames Cell Events: 7397 mean_circ: 0.926827
Ran for 773050 frames Cell Events: 7400 mean_circ: 0.926834
Ran for 774050 frames Cell Events: 7411 mean_circ: 0.926844
Ran for 774550 frames Cell Events: 7414 mean_circ: 0.926855
Ran for 775050 frames Cell Events: 7416 mean_circ: 0.926858
Ran for 775550 frames Cell Events: 7421 mean_circ: 0.926867
Ran for 776550 frames Cell Events: 7426 mean_circ: 0.926875
Ran for 777050 frames Cell Events: 7431 mean_circ: 0.926863
Ran for 777550 frames Cell Events: 7434 mean_circ: 0.926864
Ran for 778050 frames Cell Events: 7441 mean_circ: 0.926844
Ran for 778550 frames Cell Events: 7445 mean_circ: 0.926845
Ran for 779550 frames Cell Events: 7458 mean_circ: 0.926857
Ran for 780050 frames Cell Events: 7465 mean_circ: 0.926894
Ran for 780550 frames Cell Events: 7466 mean_circ: 0.926899
Ran for 781050 frames Cell Events: 7468 mean_circ: 0.926905
Ran for 781550 frames Cell Events: 7472 mean_circ: 0.926917
Ran for 782050 frames Cell Events: 7477 mean_circ: 0.926908
Ran for 782550 frames Cell Events: 7480 mean_circ: 0.926911
Ran for 783050 frames Cell Events: 7484 mean_circ: 0.926904
Ran for 783550 frames Cell Events: 7491 mean_circ: 0.926889
Ran for 784050 frames Cell Events: 7496 mean_circ: 0.926901
Ran for 784550 frames Cell Events: 7499 mean_circ: 0.926876
Ran for 785050 frames Cell Events: 7504 mean_circ: 0.926858
Ran for 785550 frames Cell Events: 7507 mean_circ: 0.926877
Ran for 786050 frames Cell Events: 7509 mean_circ: 0.926868
Ran for 786550 frames Cell Events: 7513 mean_circ: 0.926857
Ran for 787050 frames Cell Events: 7516 mean_circ: 0.926863
Ran for 788050 frames Cell Events: 7521 mean_circ: 0.926878
Ran for 788550 frames Cell Events: 7526 mean_circ: 0.926869
Ran for 789050 frames Cell Events: 7530 mean_circ: 0.926877
Ran for 789550 frames Cell Events: 7535 mean_circ: 0.926899
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Ran for 791050 frames Cell Events: 7550 mean_circ: 0.926922
Ran for 791550 frames Cell Events: 7551 mean_circ: 0.926929
Ran for 792050 frames Cell Events: 7555 mean_circ: 0.926933
Ran for 792550 frames Cell Events: 7559 mean_circ: 0.926931
Ran for 793050 frames Cell Events: 7564 mean_circ: 0.926935
Ran for 793550 frames Cell Events: 7568 mean_circ: 0.926942
Ran for 794050 frames Cell Events: 7575 mean_circ: 0.926938
Ran for 794550 frames Cell Events: 7578 mean_circ: 0.926928
Ran for 795050 frames Cell Events: 7585 mean_circ: 0.926911
Ran for 795550 frames Cell Events: 7590 mean_circ: 0.926933
Ran for 796550 frames Cell Events: 7597 mean_circ: 0.926939
Ran for 797050 frames Cell Events: 7603 mean_circ: 0.926931
Ran for 797550 frames Cell Events: 7611 mean_circ: 0.926947
Ran for 798550 frames Cell Events: 7621 mean_circ: 0.92696
Ran for 799050 frames Cell Events: 7625 mean_circ: 0.926959
Ran for 799550 frames Cell Events: 7631 mean_circ: 0.926947
Ran for 800050 frames Cell Events: 7637 mean_circ: 0.926927
Ran for 800550 frames Cell Events: 7643 mean_circ: 0.926936
Ran for 801050 frames Cell Events: 7654 mean_circ: 0.926943
Ran for 802050 frames Cell Events: 7672 mean_circ: 0.926957
Ran for 802550 frames Cell Events: 7677 mean_circ: 0.926962
Ran for 803050 frames Cell Events: 7680 mean_circ: 0.92691
Ran for 803550 frames Cell Events: 7682 mean_circ: 0.926901
Ran for 804050 frames Cell Events: 7688 mean_circ: 0.926897
Ran for 804550 frames Cell Events: 7692 mean_circ: 0.926884
Ran for 805050 frames Cell Events: 7697 mean_circ: 0.926897
Ran for 805550 frames Cell Events: 7699 mean_circ: 0.926883
Ran for 806050 frames Cell Events: 7701 mean_circ: 0.926882
Ran for 806550 frames Cell Events: 7704 mean_circ: 0.926892
Ran for 807050 frames Cell Events: 7709 mean_circ: 0.926893
Ran for 807550 frames Cell Events: 7718 mean_circ: 0.926909
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Ran for 809550 frames Cell Events: 7734 mean_circ: 0.926943
Ran for 810050 frames Cell Events: 7740 mean_circ: 0.926954
Ran for 810550 frames Cell Events: 7742 mean_circ: 0.926942
Ran for 811050 frames Cell Events: 7744 mean_circ: 0.926948
Ran for 811550 frames Cell Events: 7748 mean_circ: 0.92695
Ran for 812050 frames Cell Events: 7749 mean_circ: 0.926936
Ran for 812550 frames Cell Events: 7752 mean_circ: 0.926907
Ran for 813050 frames Cell Events: 7756 mean_circ: 0.926913
Ran for 813550 frames Cell Events: 7760 mean_circ: 0.926882
Ran for 814050 frames Cell Events: 7763 mean_circ: 0.926886
Ran for 815050 frames Cell Events: 7772 mean_circ: 0.926889
Ran for 815550 frames Cell Events: 7775 mean_circ: 0.926894
Ran for 816050 frames Cell Events: 7777 mean_circ: 0.926899
Ran for 816550 frames Cell Events: 7782 mean_circ: 0.926913
Ran for 817050 frames Cell Events: 7785 mean_circ: 0.926915
Ran for 817550 frames Cell Events: 7789 mean_circ: 0.926917
Ran for 818050 frames Cell Events: 7796 mean_circ: 0.926945
Ran for 819050 frames Cell Events: 7804 mean_circ: 0.926954
Ran for 819550 frames Cell Events: 7811 mean_circ: 0.92694
Ran for 820050 frames Cell Events: 7819 mean_circ: 0.926912
Ran for 820550 frames Cell Events: 7821 mean_circ: 0.926898
Ran for 821050 frames Cell Events: 7829 mean_circ: 0.926905
Ran for 821550 frames Cell Events: 7834 mean_circ: 0.926905
Ran for 822050 frames Cell Events: 7839 mean_circ: 0.926911
Ran for 822550 frames Cell Events: 7843 mean_circ: 0.926905
Ran for 823050 frames Cell Events: 7846 mean_circ: 0.926898
Ran for 823550 frames Cell Events: 7853 mean_circ: 0.926902
Ran for 824050 frames Cell Events: 7858 mean_circ: 0.926894
Ran for 824550 frames Cell Events: 7866 mean_circ: 0.926878
Ran for 825050 frames Cell Events: 7872 mean_circ: 0.926887
Ran for 825550 frames Cell Events: 7874 mean_circ: 0.926888
Ran for 826050 frames Cell Events: 7878 mean_circ: 0.92688
Ran for 826550 frames Cell Events: 7880 mean_circ: 0.926878
Ran for 827050 frames Cell Events: 7882 mean_circ: 0.926884
Ran for 827550 frames Cell Events: 7884 mean_circ: 0.926892
Ran for 828050 frames Cell Events: 7887 mean_circ: 0.9269
Ran for 828550 frames Cell Events: 7890 mean_circ: 0.926893
Ran for 829050 frames Cell Events: 7895 mean_circ: 0.926908
Ran for 829550 frames Cell Events: 7901 mean_circ: 0.926909
Ran for 830050 frames Cell Events: 7904 mean_circ: 0.926918
Ran for 830550 frames Cell Events: 7908 mean_circ: 0.9269
Ran for 831550 frames Cell Events: 7916 mean_circ: 0.92692
Ran for 832050 frames Cell Events: 7922 mean_circ: 0.926916
Ran for 832550 frames Cell Events: 7923 mean_circ: 0.92691
Ran for 833050 frames Cell Events: 7929 mean_circ: 0.926924
Ran for 833550 frames Cell Events: 7937 mean_circ: 0.926931
Ran for 834050 frames Cell Events: 7941 mean_circ: 0.926931
Ran for 835050 frames Cell Events: 7955 mean_circ: 0.926899
Ran for 835550 frames Cell Events: 7958 mean_circ: 0.926904
Ran for 836050 frames Cell Events: 7960 mean_circ: 0.926914
Ran for 836550 frames Cell Events: 7963 mean_circ: 0.926909
Ran for 837050 frames Cell Events: 7967 mean_circ: 0.926906
Ran for 837550 frames Cell Events: 7972 mean_circ: 0.926912
Ran for 838050 frames Cell Events: 7974 mean_circ: 0.926923
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Ran for 840050 frames Cell Events: 7988 mean_circ: 0.926924
Ran for 840550 frames Cell Events: 7992 mean_circ: 0.926923
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Ran for 842050 frames Cell Events: 8004 mean_circ: 0.926897
Ran for 842550 frames Cell Events: 8008 mean_circ: 0.926887
Ran for 843050 frames Cell Events: 8013 mean_circ: 0.926892
Ran for 843550 frames Cell Events: 8016 mean_circ: 0.926896
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Ran for 847550 frames Cell Events: 8051 mean_circ: 0.926909
Ran for 848050 frames Cell Events: 8054 mean_circ: 0.926907
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Ran for 849550 frames Cell Events: 8077 mean_circ: 0.926951
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Ran for 853050 frames Cell Events: 8106 mean_circ: 0.926984
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Ran for 854050 frames Cell Events: 8112 mean_circ: 0.926992
Ran for 854550 frames Cell Events: 8118 mean_circ: 0.926977
Ran for 855050 frames Cell Events: 8125 mean_circ: 0.926983
Ran for 855550 frames Cell Events: 8128 mean_circ: 0.926988
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Ran for 856550 frames Cell Events: 8136 mean_circ: 0.927004
Ran for 857050 frames Cell Events: 8140 mean_circ: 0.927014
Ran for 857550 frames Cell Events: 8143 mean_circ: 0.927017
Ran for 858050 frames Cell Events: 8146 mean_circ: 0.927027
Ran for 858550 frames Cell Events: 8150 mean_circ: 0.927025
Ran for 859050 frames Cell Events: 8152 mean_circ: 0.92703
Ran for 860050 frames Cell Events: 8158 mean_circ: 0.927022
Ran for 860550 frames Cell Events: 8165 mean_circ: 0.92703
Ran for 861050 frames Cell Events: 8169 mean_circ: 0.927042
Ran for 862050 frames Cell Events: 8179 mean_circ: 0.927025
Ran for 862550 frames Cell Events: 8187 mean_circ: 0.92704
Ran for 863050 frames Cell Events: 8194 mean_circ: 0.92701
Ran for 863550 frames Cell Events: 8200 mean_circ: 0.92701
Ran for 864050 frames Cell Events: 8202 mean_circ: 0.927008
Ran for 864550 frames Cell Events: 8205 mean_circ: 0.927
Ran for 865050 frames Cell Events: 8206 mean_circ: 0.926999
End this measurment..
Stopping time: 7.32668e+008
****************************************
Measurement finished
Mean Circularitiy: 0.927001
Mean Area: 989.465
Mean FL-1 intensity: 5553.41
Mean FL-2 intensity: 7058.47
Mean FL-3 intensity: 0
frames processed: 861624
Total Time: 432779ms
Number of Runs (new bg img) 0
Frames collected: 865394
Skipped frames: 149808
Rescued Frames: 5021774
Samples DAQ: 216348487
Samples written: 432696782
Samples per Channel: 216348391
FPS: 1999.62
Samples per second: 499905
Expected samples/s: 500000
Events: 8207
Events per second: 18.9635
jumped back (PMT): 6924
#Frames read, frequency
# 0 frames :1093593
# 1 frames :598645
# 2 frames :10197
# 3 frames :4531
# 4 frames :3680
# 5 frames :2038
# 6 frames :2229
# 7 frames :2889
# 8 frames :2258
# 9 frames :1458
# 10 frames :937
# 11 frames :736
# 12 frames :1139
# 13 frames :1171
# 14 frames :1294
# 15 frames :915
# 16 frames :682
# 17 frames :495
# 18 frames :297
# 19 frames :241
# 20 frames :212
# 21 frames :206
# 22 frames :192
# 23 frames :143
# 24 frames :149
# 25 frames :116
# 26 frames :71
# 27 frames :50
# 28 frames :35
# 29 frames :19
# 30 frames :18
# 31 frames :17
# 32 frames :16
# 33 frames :12
# 34 frames :9
# 35 frames :11
# 36 frames :11
# 37 frames :7
# 38 frames :2
# 39 frames :5
# 40 frames :2
# 41 frames :7
# 43 frames :1
# 44 frames :1
# 45 frames :4
# 47 frames :2
# 49 frames :3
# 50 frames :2
# 57 frames :1
# 71 frames :1
# 73 frames :1
M1_para.ini
[General]Measurement Number = 1
Flow Rate [ul/s] = 0.04
Region = "Channel"
Channel width = 20
Current LED = 100
[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0
#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 25.1914
parameters.txt
#fRT-DC Configuration file# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 300
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0
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Additional Information
Field | Value |
---|---|
license | Creative Commons Public Domain Dedication |
created | 3 months ago |
etag | 5d36255ae2cbf0e5b0917c819f39c821 |
format | RT-FDC |
id | d20641a0-a8ee-4420-af03-4c001ea521e9 |
metadata modified | 3 months ago |
mimetype | RT-DC |
package id | 3b6a0605-8625-4a18-b238-acf9e3a4c712 |
position | 0 |
s3 available | True |
s3 url | https://objectstore.hpccloud.mpcdf.mpg.de/circle-8a23b5b7-6d86-46b7-9833-62373e9ec110/resource/d20/641/a0-a8ee-4420-af03-4c001ea521e9 |
sha256 | e06e3dbac64d34b76f477630e425159c291deeddf4e1c6c08632ea3edeea908c |
size | 147.3 MiB |
dc:experiment:date | 2017-05-23 |
dc:experiment:event count | 8,205 |
dc:experiment:run identifier | None-9b90 |
dc:experiment:run index | 1 |
dc:experiment:sample | 20170523_1946_dsRNA_H5_col |
dc:experiment:time | 19:46:44 |
dc:fluorescence:bit depth | 16 |
dc:fluorescence:channel 1 name | FL1 |
dc:fluorescence:channel 2 name | FL2 |
dc:fluorescence:channel count | 2 |
dc:fluorescence:channels installed | 3 |
dc:fluorescence:laser 1 lambda | 488 |
dc:fluorescence:laser 1 power | 10 |
dc:fluorescence:laser 2 lambda | 561 |
dc:fluorescence:laser 2 power | 35 |
dc:fluorescence:laser 3 lambda | 640 |
dc:fluorescence:laser 3 power | 0 |
dc:fluorescence:laser count | 2 |
dc:fluorescence:lasers installed | 3 |
dc:fluorescence:sample rate | 500,000 |
dc:fluorescence:samples per event | 300 |
dc:fluorescence:signal max | 1 |
dc:fluorescence:signal min | -1 |
dc:fluorescence:trace median | 5 |
dc:imaging:flash device | LED (undefined) |
dc:imaging:flash duration | 2 |
dc:imaging:frame rate | 2,000 |
dc:imaging:pixel size | 0.34 |
dc:imaging:roi position x | 504 |
dc:imaging:roi position y | 472 |
dc:imaging:roi size x | 256 |
dc:imaging:roi size y | 96 |
dc:online contour:bin area min | 50 |
dc:online contour:bin kernel | 5 |
dc:online contour:bin threshold | 6 |
dc:online contour:image blur | 0 |
dc:online contour:no absdiff | True |
dc:setup:channel width | 20 |
dc:setup:chip region | channel |
dc:setup:flow rate | 0.04 |
dc:setup:module composition | Cell_Flow_2, Fluor |
dc:setup:software version | fRT-DC V1.06170206 | dclab 0.60.3 |
sp:cells:cell type | KC167 |
sp:cells:live | True |
sp:cells:organism | Drosophila melanogaster |
sp:chip:channel width | 20 |
sp:experiment:buffer osmolality | 325 |
sp:experiment:buffer ph | 6.8 |
sp:experiment:concentration | 5 |
sp:identifiers:labels | GFP tdTomato |
sp:identifiers:tags | NLS-GFP |