M1_20170523_1955_dsRNA_H5_faster.rtdc
Click on the log titles below to expand the log entries present in this DC resource.
dclab-export_2024-07-09_20.12.46
{"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}dclab-tdms2rtdc
{"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "e960f24110b05c5c15e333bd731c32b3",
"name": "M1_20170523_1955_dsRNA_H5_faster.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-09",
"time": "18:12:59"
}
}
dclab-tdms2rtdc-warnings
MultipleSamplesPerEventFoundin dclab.rtdc_dataset.fmt_tdms.exc line 101:
Ignoring trace data of
'\\syn03.frontend.storage.int.mpl.mpg.de\guck_division2\Members\Laura\20240219_Kasia_screen_all_folders\20170523_katarzyna_kc167_phospho_screen\20170523_1955_dsRNA_H5_faster\M1_20170523_1955_dsRNA_H5_faster_traces.tdms'
due to multiple values for samples per event: [200 400]
LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 5189 (max 5190) in '5189'.
FG_Config.mcf
[Kamerasektion]ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;
[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;
[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini
[Framerate]Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6
[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini
[EVENT LOG] fRT-DC V1.06170206Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 25.1292
Start pulse train for BG Aquisition..
Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 7.3276e+008
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 550 frames Cell Events: 4 mean_circ: 0.969077
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Ran for 375050 frames Cell Events: 3585 mean_circ: 0.918202
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Ran for 380050 frames Cell Events: 3633 mean_circ: 0.918203
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Ran for 399050 frames Cell Events: 3793 mean_circ: 0.918432
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Ran for 405050 frames Cell Events: 3850 mean_circ: 0.918348
Ran for 406050 frames Cell Events: 3855 mean_circ: 0.918359
Ran for 406550 frames Cell Events: 3857 mean_circ: 0.918358
Ran for 407050 frames Cell Events: 3862 mean_circ: 0.918346
Ran for 407550 frames Cell Events: 3868 mean_circ: 0.918324
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Ran for 408550 frames Cell Events: 3879 mean_circ: 0.918385
Ran for 409050 frames Cell Events: 3880 mean_circ: 0.918384
Ran for 409550 frames Cell Events: 3883 mean_circ: 0.918418
Ran for 410050 frames Cell Events: 3889 mean_circ: 0.918447
Ran for 410550 frames Cell Events: 3892 mean_circ: 0.91845
Ran for 411550 frames Cell Events: 3899 mean_circ: 0.918461
Ran for 412050 frames Cell Events: 3901 mean_circ: 0.918489
Ran for 412550 frames Cell Events: 3904 mean_circ: 0.918489
Ran for 413050 frames Cell Events: 3910 mean_circ: 0.918432
Ran for 413550 frames Cell Events: 3915 mean_circ: 0.918476
Ran for 414050 frames Cell Events: 3919 mean_circ: 0.918465
Ran for 414550 frames Cell Events: 3925 mean_circ: 0.91845
Ran for 415050 frames Cell Events: 3929 mean_circ: 0.918475
Ran for 415550 frames Cell Events: 3935 mean_circ: 0.918483
Ran for 416050 frames Cell Events: 3938 mean_circ: 0.91849
Ran for 416550 frames Cell Events: 3943 mean_circ: 0.918483
Ran for 417550 frames Cell Events: 3951 mean_circ: 0.91849
Ran for 418050 frames Cell Events: 3957 mean_circ: 0.918516
Ran for 418550 frames Cell Events: 3960 mean_circ: 0.918526
Ran for 419050 frames Cell Events: 3963 mean_circ: 0.918525
Ran for 419550 frames Cell Events: 3970 mean_circ: 0.918492
Ran for 420050 frames Cell Events: 3975 mean_circ: 0.918434
Ran for 420550 frames Cell Events: 3979 mean_circ: 0.91845
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Ran for 422550 frames Cell Events: 3997 mean_circ: 0.918414
Ran for 423050 frames Cell Events: 3999 mean_circ: 0.918433
Ran for 423550 frames Cell Events: 4008 mean_circ: 0.918338
Ran for 424050 frames Cell Events: 4012 mean_circ: 0.918339
Ran for 424550 frames Cell Events: 4016 mean_circ: 0.918318
Ran for 425050 frames Cell Events: 4022 mean_circ: 0.918314
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Ran for 426050 frames Cell Events: 4036 mean_circ: 0.918328
Ran for 426550 frames Cell Events: 4039 mean_circ: 0.918338
Ran for 427050 frames Cell Events: 4048 mean_circ: 0.918364
Ran for 427550 frames Cell Events: 4056 mean_circ: 0.918344
Ran for 428050 frames Cell Events: 4062 mean_circ: 0.91835
Ran for 428550 frames Cell Events: 4067 mean_circ: 0.91837
Ran for 429050 frames Cell Events: 4076 mean_circ: 0.918403
Ran for 429550 frames Cell Events: 4083 mean_circ: 0.918419
Ran for 430050 frames Cell Events: 4087 mean_circ: 0.918431
Ran for 431050 frames Cell Events: 4094 mean_circ: 0.918468
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Ran for 432050 frames Cell Events: 4106 mean_circ: 0.918467
Ran for 432550 frames Cell Events: 4111 mean_circ: 0.91849
Ran for 433050 frames Cell Events: 4120 mean_circ: 0.918436
Ran for 433550 frames Cell Events: 4128 mean_circ: 0.918472
Ran for 434050 frames Cell Events: 4132 mean_circ: 0.918443
Ran for 434550 frames Cell Events: 4134 mean_circ: 0.918417
Ran for 435050 frames Cell Events: 4140 mean_circ: 0.918423
Ran for 435550 frames Cell Events: 4144 mean_circ: 0.918436
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Ran for 437050 frames Cell Events: 4158 mean_circ: 0.918495
Ran for 438050 frames Cell Events: 4165 mean_circ: 0.91848
Ran for 438550 frames Cell Events: 4169 mean_circ: 0.918495
Ran for 439050 frames Cell Events: 4175 mean_circ: 0.918514
Ran for 439550 frames Cell Events: 4181 mean_circ: 0.918488
Ran for 440050 frames Cell Events: 4187 mean_circ: 0.918507
Ran for 440550 frames Cell Events: 4194 mean_circ: 0.918503
Ran for 441050 frames Cell Events: 4195 mean_circ: 0.918503
Ran for 441550 frames Cell Events: 4202 mean_circ: 0.918456
Ran for 442050 frames Cell Events: 4208 mean_circ: 0.918426
Ran for 442550 frames Cell Events: 4218 mean_circ: 0.918424
Ran for 443050 frames Cell Events: 4221 mean_circ: 0.918429
Ran for 443550 frames Cell Events: 4223 mean_circ: 0.918448
Ran for 444050 frames Cell Events: 4232 mean_circ: 0.918475
Ran for 444550 frames Cell Events: 4240 mean_circ: 0.918447
Ran for 445050 frames Cell Events: 4248 mean_circ: 0.918464
Ran for 445550 frames Cell Events: 4254 mean_circ: 0.918504
Ran for 446050 frames Cell Events: 4255 mean_circ: 0.918514
Ran for 446550 frames Cell Events: 4258 mean_circ: 0.918539
Ran for 447550 frames Cell Events: 4268 mean_circ: 0.918578
Ran for 448050 frames Cell Events: 4274 mean_circ: 0.918578
Ran for 448550 frames Cell Events: 4277 mean_circ: 0.918573
Ran for 449050 frames Cell Events: 4279 mean_circ: 0.918594
Ran for 449550 frames Cell Events: 4288 mean_circ: 0.918653
Ran for 450050 frames Cell Events: 4291 mean_circ: 0.918673
Ran for 450550 frames Cell Events: 4296 mean_circ: 0.918681
Ran for 451050 frames Cell Events: 4298 mean_circ: 0.918698
Ran for 451550 frames Cell Events: 4302 mean_circ: 0.9187
Ran for 453050 frames Cell Events: 4318 mean_circ: 0.918755
Ran for 453550 frames Cell Events: 4322 mean_circ: 0.918764
Ran for 454050 frames Cell Events: 4324 mean_circ: 0.918757
Ran for 455050 frames Cell Events: 4331 mean_circ: 0.918759
Ran for 455550 frames Cell Events: 4335 mean_circ: 0.918737
Ran for 456050 frames Cell Events: 4341 mean_circ: 0.918674
Ran for 456550 frames Cell Events: 4342 mean_circ: 0.918665
Ran for 457050 frames Cell Events: 4349 mean_circ: 0.91868
Ran for 457550 frames Cell Events: 4352 mean_circ: 0.918649
Ran for 458050 frames Cell Events: 4356 mean_circ: 0.918616
Ran for 458550 frames Cell Events: 4358 mean_circ: 0.918583
Ran for 459050 frames Cell Events: 4363 mean_circ: 0.918605
Ran for 459550 frames Cell Events: 4365 mean_circ: 0.918584
Ran for 460050 frames Cell Events: 4376 mean_circ: 0.918575
Ran for 460550 frames Cell Events: 4379 mean_circ: 0.918551
Ran for 461550 frames Cell Events: 4390 mean_circ: 0.918551
Ran for 462050 frames Cell Events: 4395 mean_circ: 0.918543
Ran for 462550 frames Cell Events: 4395 mean_circ: 0.918543
Ran for 463050 frames Cell Events: 4399 mean_circ: 0.918538
Ran for 463550 frames Cell Events: 4406 mean_circ: 0.91855
Ran for 464550 frames Cell Events: 4419 mean_circ: 0.918548
Ran for 465050 frames Cell Events: 4424 mean_circ: 0.918568
Ran for 465550 frames Cell Events: 4429 mean_circ: 0.918589
Ran for 466050 frames Cell Events: 4437 mean_circ: 0.918585
Ran for 466550 frames Cell Events: 4438 mean_circ: 0.918579
Ran for 467050 frames Cell Events: 4442 mean_circ: 0.918593
Ran for 467550 frames Cell Events: 4445 mean_circ: 0.918591
Ran for 468050 frames Cell Events: 4448 mean_circ: 0.918568
Ran for 468550 frames Cell Events: 4451 mean_circ: 0.918557
Ran for 469050 frames Cell Events: 4461 mean_circ: 0.918491
Ran for 469550 frames Cell Events: 4472 mean_circ: 0.918504
Ran for 470050 frames Cell Events: 4477 mean_circ: 0.918506
Ran for 470550 frames Cell Events: 4483 mean_circ: 0.918511
Ran for 471050 frames Cell Events: 4486 mean_circ: 0.918508
Ran for 471550 frames Cell Events: 4489 mean_circ: 0.91852
Ran for 472050 frames Cell Events: 4494 mean_circ: 0.918532
Ran for 472550 frames Cell Events: 4498 mean_circ: 0.918575
Ran for 473050 frames Cell Events: 4501 mean_circ: 0.918589
Ran for 473550 frames Cell Events: 4503 mean_circ: 0.91858
Ran for 474050 frames Cell Events: 4505 mean_circ: 0.918579
Ran for 474550 frames Cell Events: 4507 mean_circ: 0.918584
Ran for 475050 frames Cell Events: 4514 mean_circ: 0.91861
Ran for 475550 frames Cell Events: 4517 mean_circ: 0.918628
Ran for 476050 frames Cell Events: 4521 mean_circ: 0.918585
Ran for 476550 frames Cell Events: 4522 mean_circ: 0.918571
Ran for 477050 frames Cell Events: 4524 mean_circ: 0.918551
Ran for 478050 frames Cell Events: 4535 mean_circ: 0.91853
Ran for 478550 frames Cell Events: 4539 mean_circ: 0.918523
Ran for 479050 frames Cell Events: 4543 mean_circ: 0.918547
Ran for 479550 frames Cell Events: 4546 mean_circ: 0.918547
Ran for 480050 frames Cell Events: 4547 mean_circ: 0.918546
Ran for 481050 frames Cell Events: 4554 mean_circ: 0.91851
Ran for 482050 frames Cell Events: 4560 mean_circ: 0.918489
Ran for 482550 frames Cell Events: 4562 mean_circ: 0.918461
Ran for 483050 frames Cell Events: 4566 mean_circ: 0.918479
Ran for 483550 frames Cell Events: 4572 mean_circ: 0.918503
Ran for 484050 frames Cell Events: 4576 mean_circ: 0.918492
Ran for 484550 frames Cell Events: 4579 mean_circ: 0.918503
Ran for 485050 frames Cell Events: 4579 mean_circ: 0.918511
Ran for 485550 frames Cell Events: 4581 mean_circ: 0.918517
Ran for 486050 frames Cell Events: 4587 mean_circ: 0.918512
Ran for 486550 frames Cell Events: 4594 mean_circ: 0.918527
Ran for 487050 frames Cell Events: 4599 mean_circ: 0.918476
Ran for 487550 frames Cell Events: 4608 mean_circ: 0.918502
Ran for 488050 frames Cell Events: 4609 mean_circ: 0.918491
Ran for 488550 frames Cell Events: 4612 mean_circ: 0.918501
Ran for 489550 frames Cell Events: 4624 mean_circ: 0.918502
Ran for 490050 frames Cell Events: 4627 mean_circ: 0.918539
Ran for 490550 frames Cell Events: 4631 mean_circ: 0.918514
Ran for 491050 frames Cell Events: 4633 mean_circ: 0.918518
Ran for 491550 frames Cell Events: 4638 mean_circ: 0.918518
Ran for 492050 frames Cell Events: 4642 mean_circ: 0.91857
Ran for 492550 frames Cell Events: 4647 mean_circ: 0.918561
Ran for 493050 frames Cell Events: 4648 mean_circ: 0.918562
Ran for 493550 frames Cell Events: 4651 mean_circ: 0.918575
Ran for 494050 frames Cell Events: 4659 mean_circ: 0.918537
Ran for 494550 frames Cell Events: 4663 mean_circ: 0.918545
Ran for 495050 frames Cell Events: 4666 mean_circ: 0.91855
Ran for 495550 frames Cell Events: 4671 mean_circ: 0.918567
Ran for 496050 frames Cell Events: 4674 mean_circ: 0.918567
Ran for 496550 frames Cell Events: 4676 mean_circ: 0.918593
Ran for 497050 frames Cell Events: 4680 mean_circ: 0.91859
Ran for 497550 frames Cell Events: 4683 mean_circ: 0.918606
Ran for 498050 frames Cell Events: 4690 mean_circ: 0.918577
Ran for 498550 frames Cell Events: 4697 mean_circ: 0.91861
Ran for 499050 frames Cell Events: 4702 mean_circ: 0.918557
Ran for 499550 frames Cell Events: 4705 mean_circ: 0.918543
Ran for 500050 frames Cell Events: 4713 mean_circ: 0.918543
Ran for 500550 frames Cell Events: 4718 mean_circ: 0.918502
Ran for 501050 frames Cell Events: 4724 mean_circ: 0.918478
Ran for 501550 frames Cell Events: 4726 mean_circ: 0.918472
Ran for 502050 frames Cell Events: 4734 mean_circ: 0.918481
Ran for 502550 frames Cell Events: 4737 mean_circ: 0.918461
Ran for 503050 frames Cell Events: 4744 mean_circ: 0.918451
Ran for 503550 frames Cell Events: 4754 mean_circ: 0.918414
Ran for 504050 frames Cell Events: 4759 mean_circ: 0.918445
Ran for 504550 frames Cell Events: 4764 mean_circ: 0.918486
Ran for 505050 frames Cell Events: 4769 mean_circ: 0.918482
Ran for 506050 frames Cell Events: 4779 mean_circ: 0.918517
Ran for 507050 frames Cell Events: 4791 mean_circ: 0.918492
Ran for 507550 frames Cell Events: 4794 mean_circ: 0.918489
Ran for 508050 frames Cell Events: 4798 mean_circ: 0.918485
Ran for 509050 frames Cell Events: 4806 mean_circ: 0.91846
Ran for 509550 frames Cell Events: 4808 mean_circ: 0.918427
Ran for 510050 frames Cell Events: 4809 mean_circ: 0.91844
Ran for 510550 frames Cell Events: 4814 mean_circ: 0.918457
Ran for 511050 frames Cell Events: 4815 mean_circ: 0.918476
Ran for 511550 frames Cell Events: 4816 mean_circ: 0.918472
Ran for 512050 frames Cell Events: 4819 mean_circ: 0.918496
Ran for 512550 frames Cell Events: 4821 mean_circ: 0.918501
Ran for 513050 frames Cell Events: 4827 mean_circ: 0.918495
Ran for 513550 frames Cell Events: 4831 mean_circ: 0.918489
Ran for 514050 frames Cell Events: 4836 mean_circ: 0.918481
Ran for 514550 frames Cell Events: 4843 mean_circ: 0.91849
Ran for 515050 frames Cell Events: 4847 mean_circ: 0.918517
Ran for 515550 frames Cell Events: 4848 mean_circ: 0.918523
Ran for 516050 frames Cell Events: 4852 mean_circ: 0.918533
Ran for 516550 frames Cell Events: 4853 mean_circ: 0.918529
Ran for 517050 frames Cell Events: 4861 mean_circ: 0.918503
Ran for 517550 frames Cell Events: 4867 mean_circ: 0.918474
Ran for 518050 frames Cell Events: 4871 mean_circ: 0.918473
Ran for 518550 frames Cell Events: 4879 mean_circ: 0.918516
Ran for 519050 frames Cell Events: 4883 mean_circ: 0.918471
Ran for 519550 frames Cell Events: 4886 mean_circ: 0.918463
Ran for 520050 frames Cell Events: 4889 mean_circ: 0.918489
Ran for 520550 frames Cell Events: 4893 mean_circ: 0.918502
Ran for 521550 frames Cell Events: 4897 mean_circ: 0.918519
Ran for 522050 frames Cell Events: 4900 mean_circ: 0.918539
Ran for 522550 frames Cell Events: 4903 mean_circ: 0.918557
Ran for 523050 frames Cell Events: 4905 mean_circ: 0.918571
Ran for 523550 frames Cell Events: 4909 mean_circ: 0.918593
Ran for 524550 frames Cell Events: 4917 mean_circ: 0.918606
Ran for 525550 frames Cell Events: 4931 mean_circ: 0.918626
Ran for 526550 frames Cell Events: 4939 mean_circ: 0.918576
Ran for 527050 frames Cell Events: 4944 mean_circ: 0.918617
Ran for 527550 frames Cell Events: 4947 mean_circ: 0.918612
Ran for 528050 frames Cell Events: 4956 mean_circ: 0.918655
Ran for 528550 frames Cell Events: 4963 mean_circ: 0.918691
Ran for 529050 frames Cell Events: 4971 mean_circ: 0.91868
Ran for 529550 frames Cell Events: 4976 mean_circ: 0.918692
Ran for 530050 frames Cell Events: 4979 mean_circ: 0.918669
Ran for 531050 frames Cell Events: 4987 mean_circ: 0.918661
Ran for 532050 frames Cell Events: 5001 mean_circ: 0.918655
Ran for 532550 frames Cell Events: 5004 mean_circ: 0.918658
Ran for 533050 frames Cell Events: 5008 mean_circ: 0.918629
Ran for 534050 frames Cell Events: 5025 mean_circ: 0.918621
Ran for 534550 frames Cell Events: 5029 mean_circ: 0.91862
Ran for 535550 frames Cell Events: 5042 mean_circ: 0.918621
Ran for 536050 frames Cell Events: 5051 mean_circ: 0.918598
Ran for 536550 frames Cell Events: 5056 mean_circ: 0.918573
Ran for 537050 frames Cell Events: 5061 mean_circ: 0.918582
Ran for 537550 frames Cell Events: 5069 mean_circ: 0.918628
Ran for 538050 frames Cell Events: 5072 mean_circ: 0.918647
Ran for 538550 frames Cell Events: 5074 mean_circ: 0.918653
Ran for 539050 frames Cell Events: 5081 mean_circ: 0.918664
Ran for 539550 frames Cell Events: 5087 mean_circ: 0.918627
Ran for 540550 frames Cell Events: 5098 mean_circ: 0.918627
Ran for 541550 frames Cell Events: 5107 mean_circ: 0.918628
Ran for 542050 frames Cell Events: 5111 mean_circ: 0.918637
Ran for 542550 frames Cell Events: 5116 mean_circ: 0.918646
Ran for 543050 frames Cell Events: 5121 mean_circ: 0.918647
Ran for 543550 frames Cell Events: 5127 mean_circ: 0.918655
Ran for 545550 frames Cell Events: 5143 mean_circ: 0.91866
Ran for 546050 frames Cell Events: 5147 mean_circ: 0.918642
Ran for 546550 frames Cell Events: 5153 mean_circ: 0.918644
Ran for 547050 frames Cell Events: 5159 mean_circ: 0.918627
Ran for 547550 frames Cell Events: 5167 mean_circ: 0.91864
Ran for 548050 frames Cell Events: 5171 mean_circ: 0.918662
Ran for 548550 frames Cell Events: 5175 mean_circ: 0.918677
Ran for 549050 frames Cell Events: 5179 mean_circ: 0.918685
Ran for 549550 frames Cell Events: 5181 mean_circ: 0.91869
Ran for 550050 frames Cell Events: 5183 mean_circ: 0.918699
Ran for 550550 frames Cell Events: 5185 mean_circ: 0.918702
Ran for 551050 frames Cell Events: 5185 mean_circ: 0.918702
Ran for 551550 frames Cell Events: 5187 mean_circ: 0.918719
End this measurment..
Stopping time: 7.33036e+008
****************************************
Measurement finished
Mean Circularitiy: 0.918724
Mean Area: 1026.32
Mean FL-1 intensity: 5651.96
Mean FL-2 intensity: 8189.96
Mean FL-3 intensity: 0
frames processed: 547756
Total Time: 276000ms
Number of Runs (new bg img) 0
Frames collected: 551878
Skipped frames: 153931
Rescued Frames: 5154546
Samples DAQ: 137969579
Samples written: 275938784
Samples per Channel: 137969392
FPS: 1999.56
Samples per second: 499889
Expected samples/s: 500000
Events: 5191
Events per second: 18.808
jumped back (PMT): 10
#Frames read, frequency
# 0 frames :688741
# 1 frames :391030
# 2 frames :6317
# 3 frames :2903
# 4 frames :2253
# 5 frames :1364
# 6 frames :1614
# 7 frames :1633
# 8 frames :1500
# 9 frames :945
# 10 frames :605
# 11 frames :447
# 12 frames :680
# 13 frames :706
# 14 frames :950
# 15 frames :572
# 16 frames :438
# 17 frames :304
# 18 frames :188
# 19 frames :106
# 20 frames :80
# 21 frames :67
# 22 frames :53
# 23 frames :61
# 24 frames :42
# 25 frames :32
# 26 frames :34
# 27 frames :8
# 28 frames :9
# 29 frames :5
# 30 frames :9
# 31 frames :4
# 32 frames :6
# 33 frames :4
# 34 frames :3
# 35 frames :4
# 37 frames :1
# 38 frames :1
# 40 frames :1
# 42 frames :1
# 43 frames :2
# 44 frames :2
M1_para.ini
[General]Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100
[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0
#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 25.1292
parameters.txt
#fRT-DC Configuration file# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0
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Additional Information
Field | Value |
---|---|
license | Creative Commons Public Domain Dedication |
created | 3 months ago |
etag | a6b6a6d8b3574fd010a6e623a52b619e |
format | RT-FDC |
id | cefe86af-ac1b-438a-be5d-2841c8b6ea6c |
metadata modified | 3 months ago |
mimetype | RT-DC |
package id | 2827d564-5933-4f1a-beb7-5eee4ba1514c |
position | 0 |
s3 available | True |
s3 url | https://objectstore.hpccloud.mpcdf.mpg.de/circle-8a23b5b7-6d86-46b7-9833-62373e9ec110/resource/cef/e86/af-ac1b-438a-be5d-2841c8b6ea6c |
sha256 | f1e6f4ee9dfff2a42c2fc89756fa42dd96cfac918f31ef6bb7666067e6ce12fa |
size | 87.3 MiB |
dc:experiment:date | 2017-05-23 |
dc:experiment:event count | 5,188 |
dc:experiment:run identifier | None-1375 |
dc:experiment:run index | 1 |
dc:experiment:sample | 20170523_1955_dsRNA_H5_faster |
dc:experiment:time | 19:55:28 |
dc:fluorescence:bit depth | 16 |
dc:fluorescence:channel 1 name | FL1 |
dc:fluorescence:channel 2 name | FL2 |
dc:fluorescence:channel count | 2 |
dc:fluorescence:channels installed | 3 |
dc:fluorescence:laser 1 lambda | 488 |
dc:fluorescence:laser 1 power | 10 |
dc:fluorescence:laser 2 lambda | 561 |
dc:fluorescence:laser 2 power | 35 |
dc:fluorescence:laser 3 lambda | 640 |
dc:fluorescence:laser 3 power | 0 |
dc:fluorescence:laser count | 2 |
dc:fluorescence:lasers installed | 3 |
dc:fluorescence:sample rate | 500,000 |
dc:fluorescence:samples per event | 200 |
dc:fluorescence:signal max | 1 |
dc:fluorescence:signal min | -1 |
dc:fluorescence:trace median | 5 |
dc:imaging:flash device | LED (undefined) |
dc:imaging:flash duration | 2 |
dc:imaging:frame rate | 2,000 |
dc:imaging:pixel size | 0.34 |
dc:imaging:roi position x | 504 |
dc:imaging:roi position y | 472 |
dc:imaging:roi size x | 256 |
dc:imaging:roi size y | 96 |
dc:online contour:bin area min | 50 |
dc:online contour:bin kernel | 5 |
dc:online contour:bin threshold | 6 |
dc:online contour:image blur | 0 |
dc:online contour:no absdiff | True |
dc:setup:channel width | 20 |
dc:setup:chip region | channel |
dc:setup:flow rate | 0.06 |
dc:setup:module composition | Cell_Flow_2, Fluor |
dc:setup:software version | fRT-DC V1.06170206 | dclab 0.60.3 |
sp:cells:cell type | KC167 |
sp:cells:live | True |
sp:cells:organism | Drosophila melanogaster |
sp:chip:channel width | 20 |
sp:experiment:buffer osmolality | 325 |
sp:experiment:buffer ph | 6.8 |
sp:experiment:concentration | 5 |
sp:identifiers:labels | GFP tdTomato |
sp:identifiers:tags | NLS-GFP |