M1_20170523_2018_dsrna_i1_faster.rtdc
Click on the log titles below to expand the log entries present in this DC resource.
dclab-export_2024-07-09_20.13.38
{"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}dclab-tdms2rtdc
{"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "4a9b807a4528b47f8735894f96068b1b",
"name": "M1_20170523_2018_dsrna_i1_faster.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-09",
"time": "18:13:58"
}
}
dclab-tdms2rtdc-warnings
LimitingExportSizeWarningin dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 7702 (max 7703) in '7702'.
FG_Config.mcf
[Kamerasektion]ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;
[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;
[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini
[Framerate]Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6
[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini
[EVENT LOG] fRT-DC V1.06170206Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 25.1447
Start pulse train for BG Aquisition..
Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 7.34159e+008
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 550 frames Cell Events: 10 mean_circ: 0.911324
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Ran for 359050 frames Cell Events: 5243 mean_circ: 0.929672
Ran for 359550 frames Cell Events: 5253 mean_circ: 0.929619
Ran for 360050 frames Cell Events: 5261 mean_circ: 0.92964
Ran for 360550 frames Cell Events: 5269 mean_circ: 0.929625
Ran for 361050 frames Cell Events: 5284 mean_circ: 0.929651
Ran for 361550 frames Cell Events: 5291 mean_circ: 0.929661
Ran for 362050 frames Cell Events: 5297 mean_circ: 0.92966
Ran for 362550 frames Cell Events: 5306 mean_circ: 0.929668
Ran for 363550 frames Cell Events: 5326 mean_circ: 0.929653
Ran for 364050 frames Cell Events: 5331 mean_circ: 0.929657
Ran for 364550 frames Cell Events: 5344 mean_circ: 0.92967
Ran for 365050 frames Cell Events: 5353 mean_circ: 0.929695
Ran for 365550 frames Cell Events: 5363 mean_circ: 0.929673
Ran for 366050 frames Cell Events: 5370 mean_circ: 0.929683
Ran for 366550 frames Cell Events: 5380 mean_circ: 0.92974
Ran for 367050 frames Cell Events: 5387 mean_circ: 0.929747
Ran for 367550 frames Cell Events: 5393 mean_circ: 0.929756
Ran for 368550 frames Cell Events: 5416 mean_circ: 0.929837
Ran for 369050 frames Cell Events: 5421 mean_circ: 0.929804
Ran for 369550 frames Cell Events: 5428 mean_circ: 0.929812
Ran for 370550 frames Cell Events: 5441 mean_circ: 0.929795
Ran for 371050 frames Cell Events: 5450 mean_circ: 0.92977
Ran for 372050 frames Cell Events: 5475 mean_circ: 0.929796
Ran for 372550 frames Cell Events: 5481 mean_circ: 0.929782
Ran for 373050 frames Cell Events: 5490 mean_circ: 0.929808
Ran for 373550 frames Cell Events: 5497 mean_circ: 0.929793
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Ran for 375050 frames Cell Events: 5525 mean_circ: 0.92981
Ran for 375550 frames Cell Events: 5531 mean_circ: 0.929743
Ran for 376050 frames Cell Events: 5543 mean_circ: 0.9297
Ran for 376550 frames Cell Events: 5550 mean_circ: 0.929726
Ran for 377050 frames Cell Events: 5560 mean_circ: 0.929693
Ran for 377550 frames Cell Events: 5567 mean_circ: 0.929672
Ran for 378550 frames Cell Events: 5582 mean_circ: 0.929679
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Ran for 379550 frames Cell Events: 5598 mean_circ: 0.929726
Ran for 380050 frames Cell Events: 5605 mean_circ: 0.929733
Ran for 380550 frames Cell Events: 5611 mean_circ: 0.929724
Ran for 381050 frames Cell Events: 5617 mean_circ: 0.929696
Ran for 381550 frames Cell Events: 5620 mean_circ: 0.929688
Ran for 382050 frames Cell Events: 5631 mean_circ: 0.92972
Ran for 382550 frames Cell Events: 5638 mean_circ: 0.929712
Ran for 383050 frames Cell Events: 5645 mean_circ: 0.929725
Ran for 383550 frames Cell Events: 5649 mean_circ: 0.929692
Ran for 384050 frames Cell Events: 5661 mean_circ: 0.929575
Ran for 384550 frames Cell Events: 5672 mean_circ: 0.92964
Ran for 385550 frames Cell Events: 5687 mean_circ: 0.929651
Ran for 386050 frames Cell Events: 5695 mean_circ: 0.92962
Ran for 386550 frames Cell Events: 5701 mean_circ: 0.929624
Ran for 387050 frames Cell Events: 5710 mean_circ: 0.929687
Ran for 387550 frames Cell Events: 5715 mean_circ: 0.929659
Ran for 388050 frames Cell Events: 5725 mean_circ: 0.929667
Ran for 388550 frames Cell Events: 5732 mean_circ: 0.929715
Ran for 389550 frames Cell Events: 5743 mean_circ: 0.929757
Ran for 390050 frames Cell Events: 5747 mean_circ: 0.929752
Ran for 390550 frames Cell Events: 5756 mean_circ: 0.929753
Ran for 391050 frames Cell Events: 5765 mean_circ: 0.929787
Ran for 392550 frames Cell Events: 5792 mean_circ: 0.929793
Ran for 393050 frames Cell Events: 5800 mean_circ: 0.929741
Ran for 393550 frames Cell Events: 5807 mean_circ: 0.92976
Ran for 394050 frames Cell Events: 5812 mean_circ: 0.929774
Ran for 395050 frames Cell Events: 5840 mean_circ: 0.929823
Ran for 395550 frames Cell Events: 5854 mean_circ: 0.929884
Ran for 397050 frames Cell Events: 5893 mean_circ: 0.929893
Ran for 398050 frames Cell Events: 5906 mean_circ: 0.929897
Ran for 399050 frames Cell Events: 5923 mean_circ: 0.929867
Ran for 399550 frames Cell Events: 5927 mean_circ: 0.929864
Ran for 400550 frames Cell Events: 5949 mean_circ: 0.9299
Ran for 401050 frames Cell Events: 5955 mean_circ: 0.929923
Ran for 402050 frames Cell Events: 5969 mean_circ: 0.929991
Ran for 402550 frames Cell Events: 5976 mean_circ: 0.92997
Ran for 403050 frames Cell Events: 5983 mean_circ: 0.929967
Ran for 403550 frames Cell Events: 5989 mean_circ: 0.929984
Ran for 404050 frames Cell Events: 5994 mean_circ: 0.92998
Ran for 404550 frames Cell Events: 6000 mean_circ: 0.929981
Ran for 405050 frames Cell Events: 6004 mean_circ: 0.92999
Ran for 405550 frames Cell Events: 6010 mean_circ: 0.930025
Ran for 406050 frames Cell Events: 6020 mean_circ: 0.930022
Ran for 406550 frames Cell Events: 6028 mean_circ: 0.930039
Ran for 407050 frames Cell Events: 6031 mean_circ: 0.93006
Ran for 407550 frames Cell Events: 6037 mean_circ: 0.930053
Ran for 408050 frames Cell Events: 6048 mean_circ: 0.930058
Ran for 408550 frames Cell Events: 6054 mean_circ: 0.930028
Ran for 409050 frames Cell Events: 6062 mean_circ: 0.930048
Ran for 409550 frames Cell Events: 6069 mean_circ: 0.930077
Ran for 410050 frames Cell Events: 6076 mean_circ: 0.930103
Ran for 410550 frames Cell Events: 6083 mean_circ: 0.930135
Ran for 411050 frames Cell Events: 6092 mean_circ: 0.93017
Ran for 411550 frames Cell Events: 6100 mean_circ: 0.930186
Ran for 412050 frames Cell Events: 6107 mean_circ: 0.930155
Ran for 413050 frames Cell Events: 6131 mean_circ: 0.930101
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Ran for 414050 frames Cell Events: 6148 mean_circ: 0.930087
Ran for 414550 frames Cell Events: 6155 mean_circ: 0.930097
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Ran for 415550 frames Cell Events: 6181 mean_circ: 0.930162
Ran for 416050 frames Cell Events: 6199 mean_circ: 0.930169
Ran for 416550 frames Cell Events: 6208 mean_circ: 0.930125
Ran for 417050 frames Cell Events: 6215 mean_circ: 0.930125
Ran for 417550 frames Cell Events: 6225 mean_circ: 0.930155
Ran for 418050 frames Cell Events: 6237 mean_circ: 0.9302
Ran for 418550 frames Cell Events: 6248 mean_circ: 0.930208
Ran for 419050 frames Cell Events: 6258 mean_circ: 0.930199
Ran for 419550 frames Cell Events: 6264 mean_circ: 0.930222
Ran for 420050 frames Cell Events: 6271 mean_circ: 0.930222
Ran for 420550 frames Cell Events: 6279 mean_circ: 0.930219
Ran for 422050 frames Cell Events: 6305 mean_circ: 0.930282
Ran for 422550 frames Cell Events: 6315 mean_circ: 0.930276
Ran for 423050 frames Cell Events: 6326 mean_circ: 0.9303
Ran for 423550 frames Cell Events: 6332 mean_circ: 0.930319
Ran for 424550 frames Cell Events: 6353 mean_circ: 0.930309
Ran for 425050 frames Cell Events: 6363 mean_circ: 0.930337
Ran for 426050 frames Cell Events: 6382 mean_circ: 0.930318
Ran for 426550 frames Cell Events: 6392 mean_circ: 0.930319
Ran for 427050 frames Cell Events: 6395 mean_circ: 0.930305
Ran for 427550 frames Cell Events: 6402 mean_circ: 0.930349
Ran for 428050 frames Cell Events: 6409 mean_circ: 0.930358
Ran for 428550 frames Cell Events: 6414 mean_circ: 0.930322
Ran for 429050 frames Cell Events: 6422 mean_circ: 0.930299
Ran for 429550 frames Cell Events: 6432 mean_circ: 0.930262
Ran for 430550 frames Cell Events: 6450 mean_circ: 0.930281
Ran for 431050 frames Cell Events: 6455 mean_circ: 0.930285
Ran for 431550 frames Cell Events: 6459 mean_circ: 0.930273
Ran for 432050 frames Cell Events: 6466 mean_circ: 0.930291
Ran for 433050 frames Cell Events: 6474 mean_circ: 0.930309
Ran for 433550 frames Cell Events: 6480 mean_circ: 0.930286
Ran for 434050 frames Cell Events: 6485 mean_circ: 0.93029
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Ran for 435050 frames Cell Events: 6500 mean_circ: 0.930296
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Ran for 437550 frames Cell Events: 6548 mean_circ: 0.930287
Ran for 438050 frames Cell Events: 6554 mean_circ: 0.930234
Ran for 438550 frames Cell Events: 6563 mean_circ: 0.930229
Ran for 439050 frames Cell Events: 6572 mean_circ: 0.93024
Ran for 439550 frames Cell Events: 6582 mean_circ: 0.930232
Ran for 440050 frames Cell Events: 6588 mean_circ: 0.930239
Ran for 440550 frames Cell Events: 6597 mean_circ: 0.930245
Ran for 441050 frames Cell Events: 6606 mean_circ: 0.930265
Ran for 441550 frames Cell Events: 6609 mean_circ: 0.930276
Ran for 442050 frames Cell Events: 6615 mean_circ: 0.930274
Ran for 442550 frames Cell Events: 6623 mean_circ: 0.930269
Ran for 443050 frames Cell Events: 6633 mean_circ: 0.930233
Ran for 443550 frames Cell Events: 6641 mean_circ: 0.930256
Ran for 444050 frames Cell Events: 6650 mean_circ: 0.93028
Ran for 444550 frames Cell Events: 6660 mean_circ: 0.930235
Ran for 445050 frames Cell Events: 6668 mean_circ: 0.930236
Ran for 445550 frames Cell Events: 6679 mean_circ: 0.93025
Ran for 446050 frames Cell Events: 6682 mean_circ: 0.930256
Ran for 446550 frames Cell Events: 6692 mean_circ: 0.930195
Ran for 448050 frames Cell Events: 6721 mean_circ: 0.930215
Ran for 448550 frames Cell Events: 6728 mean_circ: 0.930154
Ran for 449050 frames Cell Events: 6734 mean_circ: 0.930165
Ran for 449550 frames Cell Events: 6740 mean_circ: 0.93017
Ran for 450050 frames Cell Events: 6746 mean_circ: 0.930188
Ran for 450550 frames Cell Events: 6761 mean_circ: 0.930127
Ran for 451050 frames Cell Events: 6769 mean_circ: 0.93012
Ran for 451550 frames Cell Events: 6774 mean_circ: 0.930124
Ran for 452550 frames Cell Events: 6789 mean_circ: 0.930161
Ran for 453050 frames Cell Events: 6791 mean_circ: 0.930161
Ran for 453550 frames Cell Events: 6795 mean_circ: 0.930152
Ran for 454050 frames Cell Events: 6799 mean_circ: 0.93016
Ran for 454550 frames Cell Events: 6805 mean_circ: 0.930183
Ran for 456050 frames Cell Events: 6833 mean_circ: 0.930199
Ran for 456550 frames Cell Events: 6846 mean_circ: 0.93021
Ran for 457050 frames Cell Events: 6857 mean_circ: 0.930253
Ran for 457550 frames Cell Events: 6858 mean_circ: 0.930262
Ran for 458050 frames Cell Events: 6869 mean_circ: 0.93027
Ran for 459050 frames Cell Events: 6894 mean_circ: 0.930234
Ran for 459550 frames Cell Events: 6905 mean_circ: 0.930215
Ran for 460050 frames Cell Events: 6910 mean_circ: 0.930215
Ran for 461050 frames Cell Events: 6924 mean_circ: 0.930241
Ran for 461550 frames Cell Events: 6933 mean_circ: 0.930245
Ran for 462550 frames Cell Events: 6952 mean_circ: 0.930239
Ran for 463550 frames Cell Events: 6975 mean_circ: 0.930243
Ran for 464550 frames Cell Events: 6993 mean_circ: 0.930208
Ran for 465050 frames Cell Events: 6997 mean_circ: 0.930195
Ran for 465550 frames Cell Events: 7007 mean_circ: 0.930175
Ran for 466550 frames Cell Events: 7022 mean_circ: 0.930184
Ran for 467050 frames Cell Events: 7032 mean_circ: 0.930198
Ran for 467550 frames Cell Events: 7038 mean_circ: 0.930154
Ran for 468050 frames Cell Events: 7048 mean_circ: 0.930152
Ran for 468550 frames Cell Events: 7052 mean_circ: 0.930165
Ran for 469050 frames Cell Events: 7067 mean_circ: 0.930109
Ran for 470550 frames Cell Events: 7087 mean_circ: 0.930079
Ran for 471050 frames Cell Events: 7091 mean_circ: 0.9301
Ran for 471550 frames Cell Events: 7094 mean_circ: 0.930092
Ran for 472050 frames Cell Events: 7107 mean_circ: 0.930036
Ran for 472550 frames Cell Events: 7118 mean_circ: 0.930043
Ran for 473050 frames Cell Events: 7124 mean_circ: 0.930041
Ran for 473550 frames Cell Events: 7129 mean_circ: 0.930028
Ran for 474550 frames Cell Events: 7145 mean_circ: 0.930021
Ran for 475050 frames Cell Events: 7159 mean_circ: 0.930022
Ran for 475550 frames Cell Events: 7166 mean_circ: 0.930013
Ran for 476550 frames Cell Events: 7183 mean_circ: 0.92999
Ran for 477050 frames Cell Events: 7187 mean_circ: 0.92999
Ran for 477550 frames Cell Events: 7192 mean_circ: 0.929951
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Ran for 479050 frames Cell Events: 7222 mean_circ: 0.929912
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Ran for 481550 frames Cell Events: 7259 mean_circ: 0.929794
Ran for 482050 frames Cell Events: 7266 mean_circ: 0.929822
Ran for 482550 frames Cell Events: 7276 mean_circ: 0.929829
Ran for 483050 frames Cell Events: 7279 mean_circ: 0.929848
Ran for 484550 frames Cell Events: 7306 mean_circ: 0.929886
Ran for 486050 frames Cell Events: 7339 mean_circ: 0.929919
Ran for 486550 frames Cell Events: 7346 mean_circ: 0.929926
Ran for 487050 frames Cell Events: 7359 mean_circ: 0.929922
Ran for 487550 frames Cell Events: 7370 mean_circ: 0.929927
Ran for 488050 frames Cell Events: 7376 mean_circ: 0.929931
Ran for 488550 frames Cell Events: 7384 mean_circ: 0.929943
Ran for 489550 frames Cell Events: 7403 mean_circ: 0.929971
Ran for 490050 frames Cell Events: 7417 mean_circ: 0.930006
Ran for 490550 frames Cell Events: 7424 mean_circ: 0.929989
Ran for 491050 frames Cell Events: 7430 mean_circ: 0.929996
Ran for 492050 frames Cell Events: 7448 mean_circ: 0.92999
Ran for 492550 frames Cell Events: 7453 mean_circ: 0.929979
Ran for 493050 frames Cell Events: 7456 mean_circ: 0.929981
Ran for 494050 frames Cell Events: 7475 mean_circ: 0.929951
Ran for 494550 frames Cell Events: 7484 mean_circ: 0.929976
Ran for 495050 frames Cell Events: 7490 mean_circ: 0.929996
Ran for 495550 frames Cell Events: 7494 mean_circ: 0.930007
Ran for 496050 frames Cell Events: 7498 mean_circ: 0.930005
Ran for 496550 frames Cell Events: 7511 mean_circ: 0.930026
Ran for 497050 frames Cell Events: 7519 mean_circ: 0.930034
Ran for 497550 frames Cell Events: 7523 mean_circ: 0.930028
Ran for 498550 frames Cell Events: 7541 mean_circ: 0.930032
Ran for 499050 frames Cell Events: 7548 mean_circ: 0.930056
Ran for 499550 frames Cell Events: 7558 mean_circ: 0.930077
Ran for 500050 frames Cell Events: 7563 mean_circ: 0.930058
Ran for 501050 frames Cell Events: 7576 mean_circ: 0.930094
Ran for 501550 frames Cell Events: 7579 mean_circ: 0.930105
Ran for 502050 frames Cell Events: 7583 mean_circ: 0.930108
Ran for 502550 frames Cell Events: 7595 mean_circ: 0.930121
Ran for 503050 frames Cell Events: 7602 mean_circ: 0.930125
Ran for 503550 frames Cell Events: 7607 mean_circ: 0.930125
Ran for 504050 frames Cell Events: 7612 mean_circ: 0.930133
Ran for 504550 frames Cell Events: 7617 mean_circ: 0.930142
Ran for 505050 frames Cell Events: 7632 mean_circ: 0.930134
Ran for 505550 frames Cell Events: 7640 mean_circ: 0.930099
Ran for 507050 frames Cell Events: 7659 mean_circ: 0.930094
Ran for 508050 frames Cell Events: 7671 mean_circ: 0.930093
Ran for 508550 frames Cell Events: 7679 mean_circ: 0.930097
Ran for 509050 frames Cell Events: 7687 mean_circ: 0.930072
Ran for 509550 frames Cell Events: 7694 mean_circ: 0.93008
Ran for 510050 frames Cell Events: 7700 mean_circ: 0.93008
End this measurment..
Stopping time: 7.34415e+008
****************************************
Measurement finished
Mean Circularitiy: 0.930091
Mean Area: 627.1
Mean FL-1 intensity: 3815.27
Mean FL-2 intensity: 5385.11
Mean FL-3 intensity: 0
frames processed: 510142
Total Time: 255228ms
Number of Runs (new bg img) 0
Frames collected: 510300
Skipped frames: 155648
Rescued Frames: 5488775
Samples DAQ: 127575166
Samples written: 255149784
Samples per Channel: 127574892
FPS: 1999.39
Samples per second: 499847
Expected samples/s: 500000
Events: 7703
Events per second: 30.1809
jumped back (PMT): 9
#Frames read, frequency
# 0 frames :646595
# 1 frames :348447
# 2 frames :6891
# 3 frames :3848
# 4 frames :1858
# 5 frames :735
# 6 frames :893
# 7 frames :2040
# 8 frames :1251
# 9 frames :951
# 10 frames :2673
# 11 frames :1366
# 12 frames :956
# 13 frames :572
# 14 frames :345
# 15 frames :230
# 16 frames :179
# 17 frames :151
# 18 frames :128
# 19 frames :106
# 20 frames :80
# 21 frames :61
# 22 frames :43
# 23 frames :51
# 24 frames :26
# 25 frames :23
# 26 frames :11
# 27 frames :5
# 28 frames :9
# 29 frames :6
# 30 frames :4
# 31 frames :9
# 32 frames :5
# 33 frames :6
# 34 frames :2
# 35 frames :1
# 36 frames :4
# 37 frames :2
# 38 frames :1
# 39 frames :2
# 40 frames :1
# 41 frames :1
# 43 frames :1
# 51 frames :1
# 52 frames :1
# 55 frames :1
M1_para.ini
[General]Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100
[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0
#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 25.1447
parameters.txt
#fRT-DC Configuration file# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0
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Additional Information
Field | Value |
---|---|
license | Creative Commons Public Domain Dedication |
created | 3 months ago |
etag | 7a4e8037057292b0764505515a2d6f9a |
format | RT-FDC |
id | beda1ae1-10de-4640-9a1c-7b3a7d6a2419 |
metadata modified | 3 months ago |
mimetype | RT-DC |
package id | c982385b-757e-4d20-bb47-bff67f7d537a |
position | 0 |
s3 available | True |
s3 url | https://objectstore.hpccloud.mpcdf.mpg.de/circle-8a23b5b7-6d86-46b7-9833-62373e9ec110/resource/bed/a1a/e1-10de-4640-9a1c-7b3a7d6a2419 |
sha256 | 1f7551c0c3a98ac96699b59fbef9da8382434420a399485b095cbe420878e244 |
size | 133.4 MiB |
dc:experiment:date | 2017-05-23 |
dc:experiment:event count | 7,701 |
dc:experiment:run identifier | None-1c33 |
dc:experiment:run index | 1 |
dc:experiment:sample | 20170523_2018_dsrna_i1_faster |
dc:experiment:time | 20:18:48 |
dc:fluorescence:bit depth | 16 |
dc:fluorescence:channel 1 name | FL1 |
dc:fluorescence:channel 2 name | FL2 |
dc:fluorescence:channel count | 2 |
dc:fluorescence:channels installed | 3 |
dc:fluorescence:laser 1 lambda | 488 |
dc:fluorescence:laser 1 power | 10 |
dc:fluorescence:laser 2 lambda | 561 |
dc:fluorescence:laser 2 power | 35 |
dc:fluorescence:laser 3 lambda | 640 |
dc:fluorescence:laser 3 power | 0 |
dc:fluorescence:laser count | 2 |
dc:fluorescence:lasers installed | 3 |
dc:fluorescence:sample rate | 500,000 |
dc:fluorescence:samples per event | 200 |
dc:fluorescence:signal max | 1 |
dc:fluorescence:signal min | -1 |
dc:fluorescence:trace median | 5 |
dc:imaging:flash device | LED (undefined) |
dc:imaging:flash duration | 2 |
dc:imaging:frame rate | 2,000 |
dc:imaging:pixel size | 0.34 |
dc:imaging:roi position x | 504 |
dc:imaging:roi position y | 472 |
dc:imaging:roi size x | 256 |
dc:imaging:roi size y | 96 |
dc:online contour:bin area min | 50 |
dc:online contour:bin kernel | 5 |
dc:online contour:bin threshold | 6 |
dc:online contour:image blur | 0 |
dc:online contour:no absdiff | True |
dc:setup:channel width | 20 |
dc:setup:chip region | channel |
dc:setup:flow rate | 0.06 |
dc:setup:module composition | Cell_Flow_2, Fluor |
dc:setup:software version | fRT-DC V1.06170206 | dclab 0.60.3 |
sp:cells:cell type | KC167 |
sp:cells:live | True |
sp:cells:organism | Drosophila melanogaster |
sp:chip:channel width | 20 |
sp:experiment:buffer osmolality | 325 |
sp:experiment:buffer ph | 6.8 |
sp:experiment:concentration | 5 |
sp:identifiers:labels | GFP tdTomato |
sp:identifiers:tags | NLS-GFP |