Click on the log titles below to expand the log entries present in this DC resource.
dclab-export_2024-07-10_11.32.24
{"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc
{
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "63de3febdf38c445d94bd66527df9d8a",
"name": "M1_20170530_1447_dsRNA_I5_col.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "09:32:51"
}
}
dclab-tdms2rtdc-warnings
LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 9922 (max 9923) in '9922'.
FG_Config.mcf
[Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;
[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;
[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini
[Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6
[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini
[EVENT LOG] fRT-DC V1.06170206
Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 24.6967
Start pulse train for BG Aquisition..
Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 1.31906e+009
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 1050 frames Cell Events: 35 mean_circ: 0.95797
Ran for 1550 frames Cell Events: 46 mean_circ: 0.956007
Ran for 2050 frames Cell Events: 56 mean_circ: 0.955928
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Ran for 83050 frames Cell Events: 2692 mean_circ: 0.943319
Ran for 83550 frames Cell Events: 2713 mean_circ: 0.943302
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Ran for 85050 frames Cell Events: 2765 mean_circ: 0.94325
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Ran for 86550 frames Cell Events: 2824 mean_circ: 0.943082
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Ran for 92550 frames Cell Events: 3024 mean_circ: 0.943093
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Ran for 102050 frames Cell Events: 3383 mean_circ: 0.942955
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Ran for 122050 frames Cell Events: 4074 mean_circ: 0.943078
Ran for 122550 frames Cell Events: 4097 mean_circ: 0.943145
Ran for 123550 frames Cell Events: 4135 mean_circ: 0.943095
Ran for 124050 frames Cell Events: 4148 mean_circ: 0.943124
Ran for 125050 frames Cell Events: 4178 mean_circ: 0.943141
Ran for 126550 frames Cell Events: 4227 mean_circ: 0.94315
Ran for 127550 frames Cell Events: 4272 mean_circ: 0.94326
Ran for 128550 frames Cell Events: 4305 mean_circ: 0.94325
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Ran for 132050 frames Cell Events: 4417 mean_circ: 0.94345
Ran for 132550 frames Cell Events: 4442 mean_circ: 0.943477
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Ran for 187050 frames Cell Events: 6378 mean_circ: 0.944236
Ran for 187550 frames Cell Events: 6399 mean_circ: 0.94418
Ran for 188550 frames Cell Events: 6456 mean_circ: 0.944177
Ran for 189050 frames Cell Events: 6470 mean_circ: 0.944181
Ran for 189550 frames Cell Events: 6488 mean_circ: 0.944131
Ran for 190550 frames Cell Events: 6506 mean_circ: 0.944153
Ran for 191050 frames Cell Events: 6540 mean_circ: 0.944141
Ran for 192550 frames Cell Events: 6595 mean_circ: 0.943978
Ran for 193050 frames Cell Events: 6614 mean_circ: 0.943953
Ran for 193550 frames Cell Events: 6637 mean_circ: 0.943929
Ran for 195050 frames Cell Events: 6699 mean_circ: 0.943927
Ran for 195550 frames Cell Events: 6722 mean_circ: 0.943925
Ran for 196550 frames Cell Events: 6761 mean_circ: 0.943926
Ran for 197050 frames Cell Events: 6776 mean_circ: 0.943972
Ran for 198050 frames Cell Events: 6814 mean_circ: 0.943959
Ran for 198550 frames Cell Events: 6826 mean_circ: 0.943938
Ran for 199050 frames Cell Events: 6845 mean_circ: 0.943957
Ran for 200050 frames Cell Events: 6898 mean_circ: 0.943847
Ran for 201050 frames Cell Events: 6936 mean_circ: 0.943945
Ran for 202050 frames Cell Events: 6989 mean_circ: 0.943933
Ran for 202550 frames Cell Events: 7016 mean_circ: 0.94394
Ran for 204050 frames Cell Events: 7070 mean_circ: 0.943971
Ran for 207050 frames Cell Events: 7192 mean_circ: 0.944072
Ran for 207550 frames Cell Events: 7216 mean_circ: 0.944089
Ran for 208050 frames Cell Events: 7231 mean_circ: 0.944088
Ran for 208550 frames Cell Events: 7254 mean_circ: 0.944056
Ran for 209050 frames Cell Events: 7264 mean_circ: 0.944066
Ran for 210050 frames Cell Events: 7329 mean_circ: 0.944075
Ran for 210550 frames Cell Events: 7352 mean_circ: 0.944109
Ran for 211050 frames Cell Events: 7372 mean_circ: 0.944111
Ran for 212550 frames Cell Events: 7430 mean_circ: 0.944107
Ran for 213050 frames Cell Events: 7447 mean_circ: 0.944084
Ran for 213550 frames Cell Events: 7461 mean_circ: 0.944065
Ran for 214050 frames Cell Events: 7483 mean_circ: 0.944073
Ran for 214550 frames Cell Events: 7494 mean_circ: 0.944094
Ran for 215050 frames Cell Events: 7513 mean_circ: 0.944112
Ran for 216050 frames Cell Events: 7555 mean_circ: 0.94414
Ran for 216550 frames Cell Events: 7574 mean_circ: 0.944124
Ran for 217550 frames Cell Events: 7612 mean_circ: 0.944077
Ran for 218050 frames Cell Events: 7633 mean_circ: 0.944072
Ran for 220050 frames Cell Events: 7723 mean_circ: 0.944069
Ran for 220550 frames Cell Events: 7743 mean_circ: 0.944042
Ran for 222550 frames Cell Events: 7824 mean_circ: 0.943997
Ran for 223550 frames Cell Events: 7865 mean_circ: 0.944047
Ran for 224050 frames Cell Events: 7884 mean_circ: 0.944068
Ran for 224550 frames Cell Events: 7914 mean_circ: 0.944099
Ran for 225050 frames Cell Events: 7937 mean_circ: 0.944027
Ran for 225550 frames Cell Events: 7955 mean_circ: 0.944045
Ran for 226050 frames Cell Events: 7976 mean_circ: 0.944061
Ran for 226550 frames Cell Events: 7999 mean_circ: 0.944011
Ran for 228550 frames Cell Events: 8082 mean_circ: 0.94398
Ran for 229050 frames Cell Events: 8101 mean_circ: 0.943982
Ran for 229550 frames Cell Events: 8134 mean_circ: 0.943985
Ran for 230050 frames Cell Events: 8154 mean_circ: 0.944017
Ran for 230550 frames Cell Events: 8177 mean_circ: 0.944016
Ran for 231050 frames Cell Events: 8193 mean_circ: 0.944033
Ran for 232050 frames Cell Events: 8237 mean_circ: 0.944031
Ran for 232550 frames Cell Events: 8256 mean_circ: 0.944079
Ran for 233050 frames Cell Events: 8272 mean_circ: 0.94408
Ran for 234050 frames Cell Events: 8309 mean_circ: 0.944064
Ran for 234550 frames Cell Events: 8327 mean_circ: 0.9441
Ran for 235050 frames Cell Events: 8357 mean_circ: 0.944096
Ran for 235550 frames Cell Events: 8381 mean_circ: 0.94413
Ran for 236050 frames Cell Events: 8397 mean_circ: 0.944151
Ran for 236550 frames Cell Events: 8423 mean_circ: 0.944179
Ran for 237050 frames Cell Events: 8435 mean_circ: 0.944177
Ran for 238050 frames Cell Events: 8481 mean_circ: 0.944135
Ran for 238550 frames Cell Events: 8504 mean_circ: 0.944177
Ran for 240050 frames Cell Events: 8566 mean_circ: 0.944119
Ran for 240550 frames Cell Events: 8603 mean_circ: 0.944112
Ran for 241550 frames Cell Events: 8632 mean_circ: 0.944123
Ran for 242050 frames Cell Events: 8650 mean_circ: 0.94413
Ran for 242550 frames Cell Events: 8659 mean_circ: 0.944152
Ran for 243050 frames Cell Events: 8679 mean_circ: 0.944149
Ran for 243550 frames Cell Events: 8692 mean_circ: 0.944149
Ran for 244050 frames Cell Events: 8711 mean_circ: 0.944132
Ran for 244550 frames Cell Events: 8732 mean_circ: 0.944155
Ran for 245050 frames Cell Events: 8767 mean_circ: 0.944155
Ran for 245550 frames Cell Events: 8788 mean_circ: 0.944112
Ran for 246550 frames Cell Events: 8834 mean_circ: 0.94414
Ran for 247550 frames Cell Events: 8879 mean_circ: 0.944221
Ran for 249050 frames Cell Events: 8943 mean_circ: 0.94431
Ran for 249550 frames Cell Events: 8963 mean_circ: 0.94426
Ran for 250050 frames Cell Events: 8985 mean_circ: 0.944264
Ran for 250550 frames Cell Events: 9004 mean_circ: 0.944256
Ran for 251050 frames Cell Events: 9024 mean_circ: 0.944257
Ran for 251550 frames Cell Events: 9052 mean_circ: 0.944294
Ran for 252050 frames Cell Events: 9067 mean_circ: 0.944311
Ran for 253050 frames Cell Events: 9105 mean_circ: 0.944319
Ran for 253550 frames Cell Events: 9122 mean_circ: 0.944351
Ran for 254550 frames Cell Events: 9145 mean_circ: 0.944379
Ran for 255550 frames Cell Events: 9208 mean_circ: 0.944371
Ran for 256050 frames Cell Events: 9231 mean_circ: 0.94438
Ran for 256550 frames Cell Events: 9258 mean_circ: 0.944372
Ran for 257050 frames Cell Events: 9276 mean_circ: 0.944394
Ran for 257550 frames Cell Events: 9293 mean_circ: 0.944402
Ran for 258050 frames Cell Events: 9313 mean_circ: 0.944396
Ran for 258550 frames Cell Events: 9329 mean_circ: 0.944412
Ran for 259050 frames Cell Events: 9354 mean_circ: 0.944427
Ran for 259550 frames Cell Events: 9371 mean_circ: 0.944423
Ran for 261050 frames Cell Events: 9424 mean_circ: 0.944377
Ran for 261550 frames Cell Events: 9439 mean_circ: 0.944386
Ran for 262050 frames Cell Events: 9459 mean_circ: 0.9444
Ran for 263050 frames Cell Events: 9500 mean_circ: 0.944363
Ran for 263550 frames Cell Events: 9519 mean_circ: 0.94435
Ran for 264050 frames Cell Events: 9537 mean_circ: 0.944351
Ran for 265550 frames Cell Events: 9603 mean_circ: 0.944387
Ran for 266050 frames Cell Events: 9621 mean_circ: 0.944382
Ran for 266550 frames Cell Events: 9637 mean_circ: 0.944375
Ran for 267050 frames Cell Events: 9669 mean_circ: 0.944394
Ran for 267550 frames Cell Events: 9690 mean_circ: 0.944381
Ran for 268050 frames Cell Events: 9713 mean_circ: 0.944415
Ran for 269550 frames Cell Events: 9767 mean_circ: 0.944427
Ran for 270050 frames Cell Events: 9793 mean_circ: 0.944414
Ran for 270550 frames Cell Events: 9811 mean_circ: 0.944413
Ran for 272050 frames Cell Events: 9880 mean_circ: 0.944365
Ran for 272550 frames Cell Events: 9913 mean_circ: 0.944363
End this measurment..
Stopping time: 1.3192e+009
****************************************
Measurement finished
Mean Circularitiy: 0.944369
Mean Area: 588.714
Mean FL-1 intensity: 2781.46
Mean FL-2 intensity: 3330.41
Mean FL-3 intensity: 0
frames processed: 270349
Total Time: 136461ms
Number of Runs (new bg img) 0
Frames collected: 272737
Skipped frames: 21560
Rescued Frames: 1054140
Samples DAQ: 68185069
Samples written: 136369210
Samples per Channel: 68184605
FPS: 1998.64
Samples per second: 499664
Expected samples/s: 500000
Events: 9923
Events per second: 72.7168
jumped back (PMT): 6963
#Frames read, frequency
# 0 frames :369148
# 1 frames :156518
# 2 frames :5892
# 3 frames :1837
# 4 frames :744
# 5 frames :241
# 6 frames :4026
# 7 frames :2112
# 8 frames :1588
# 9 frames :682
# 10 frames :663
# 11 frames :343
# 12 frames :438
# 13 frames :202
# 14 frames :270
# 15 frames :165
# 16 frames :129
# 17 frames :90
# 18 frames :56
# 19 frames :27
# 20 frames :44
# 21 frames :27
# 22 frames :33
# 23 frames :22
# 24 frames :16
# 25 frames :8
# 26 frames :10
# 27 frames :3
# 28 frames :8
# 29 frames :7
# 31 frames :9
# 32 frames :2
# 34 frames :1
# 35 frames :3
# 36 frames :1
# 37 frames :1
# 39 frames :2
# 40 frames :1
# 41 frames :2
# 42 frames :1
# 44 frames :1
# 46 frames :1
# 52 frames :1
# 67 frames :1
# 72 frames :1
# 74 frames :1
# 77 frames :1
M1_para.ini
[General]
Measurement Number = 1
Flow Rate [ul/s] = 0.04
Region = "Channel"
Channel width = 20
Current LED = 100
[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0
#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 24.6967
parameters.txt
#fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 300
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0