Click on the log titles below to expand the log entries present in this DC resource.
dclab-export_2024-07-10_11.40.19
{"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc
{
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "367844fc369b43accbf2b4ca97f1d5a6",
"name": "M1_20170530_1612_dsRNA_J7_faster.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "09:40:40"
}
}
dclab-tdms2rtdc-warnings
LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8229 (max 8230) in '8229'.
FG_Config.mcf
[Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;
[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;
[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini
[Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6
[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini
[EVENT LOG] fRT-DC V1.06170206
Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 24.9048
Start pulse train for BG Aquisition..
Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 1.32416e+009
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 550 frames Cell Events: 25 mean_circ: 0.927062
Ran for 2550 frames Cell Events: 120 mean_circ: 0.928829
Ran for 3050 frames Cell Events: 159 mean_circ: 0.93187
Ran for 3550 frames Cell Events: 194 mean_circ: 0.931194
Ran for 5550 frames Cell Events: 303 mean_circ: 0.929832
Ran for 6050 frames Cell Events: 336 mean_circ: 0.929757
Ran for 6550 frames Cell Events: 359 mean_circ: 0.929616
Ran for 8050 frames Cell Events: 453 mean_circ: 0.932164
Ran for 8550 frames Cell Events: 471 mean_circ: 0.932715
Ran for 9550 frames Cell Events: 529 mean_circ: 0.932993
Ran for 10550 frames Cell Events: 575 mean_circ: 0.932506
Ran for 11050 frames Cell Events: 595 mean_circ: 0.932718
Ran for 11550 frames Cell Events: 615 mean_circ: 0.932218
Ran for 12050 frames Cell Events: 644 mean_circ: 0.932218
Ran for 12550 frames Cell Events: 659 mean_circ: 0.932449
Ran for 14050 frames Cell Events: 719 mean_circ: 0.932614
Ran for 14550 frames Cell Events: 743 mean_circ: 0.932861
Ran for 15550 frames Cell Events: 788 mean_circ: 0.933531
Ran for 16050 frames Cell Events: 806 mean_circ: 0.933918
Ran for 16550 frames Cell Events: 836 mean_circ: 0.934178
Ran for 17550 frames Cell Events: 882 mean_circ: 0.934672
Ran for 18050 frames Cell Events: 900 mean_circ: 0.934452
Ran for 19050 frames Cell Events: 954 mean_circ: 0.934328
Ran for 19550 frames Cell Events: 977 mean_circ: 0.93437
Ran for 20550 frames Cell Events: 1031 mean_circ: 0.934278
Ran for 21050 frames Cell Events: 1045 mean_circ: 0.934377
Ran for 22050 frames Cell Events: 1099 mean_circ: 0.93474
Ran for 22550 frames Cell Events: 1124 mean_circ: 0.934767
Ran for 23050 frames Cell Events: 1154 mean_circ: 0.934626
Ran for 23550 frames Cell Events: 1182 mean_circ: 0.934573
Ran for 25550 frames Cell Events: 1290 mean_circ: 0.934706
Ran for 26050 frames Cell Events: 1320 mean_circ: 0.934708
Ran for 27050 frames Cell Events: 1379 mean_circ: 0.934881
Ran for 28550 frames Cell Events: 1469 mean_circ: 0.934446
Ran for 29050 frames Cell Events: 1491 mean_circ: 0.93447
Ran for 29550 frames Cell Events: 1523 mean_circ: 0.934455
Ran for 32550 frames Cell Events: 1696 mean_circ: 0.9345
Ran for 33550 frames Cell Events: 1750 mean_circ: 0.934597
Ran for 34050 frames Cell Events: 1772 mean_circ: 0.93444
Ran for 34550 frames Cell Events: 1789 mean_circ: 0.934541
Ran for 35050 frames Cell Events: 1813 mean_circ: 0.934297
Ran for 36050 frames Cell Events: 1856 mean_circ: 0.934075
Ran for 36550 frames Cell Events: 1883 mean_circ: 0.934005
Ran for 37050 frames Cell Events: 1899 mean_circ: 0.933862
Ran for 37550 frames Cell Events: 1929 mean_circ: 0.933902
Ran for 40550 frames Cell Events: 2105 mean_circ: 0.933428
Ran for 41550 frames Cell Events: 2165 mean_circ: 0.933527
Ran for 42050 frames Cell Events: 2193 mean_circ: 0.933382
Ran for 42550 frames Cell Events: 2213 mean_circ: 0.933314
Ran for 43050 frames Cell Events: 2241 mean_circ: 0.93343
Ran for 46050 frames Cell Events: 2416 mean_circ: 0.933589
Ran for 47050 frames Cell Events: 2479 mean_circ: 0.933736
Ran for 47550 frames Cell Events: 2496 mean_circ: 0.93359
Ran for 48050 frames Cell Events: 2518 mean_circ: 0.933594
Ran for 48550 frames Cell Events: 2553 mean_circ: 0.93328
Ran for 50050 frames Cell Events: 2625 mean_circ: 0.933518
Ran for 50550 frames Cell Events: 2649 mean_circ: 0.933537
Ran for 51050 frames Cell Events: 2676 mean_circ: 0.933395
Ran for 52050 frames Cell Events: 2718 mean_circ: 0.933359
Ran for 53050 frames Cell Events: 2773 mean_circ: 0.933317
Ran for 54050 frames Cell Events: 2827 mean_circ: 0.933403
Ran for 54550 frames Cell Events: 2848 mean_circ: 0.933412
Ran for 55050 frames Cell Events: 2879 mean_circ: 0.933453
Ran for 55550 frames Cell Events: 2909 mean_circ: 0.933507
Ran for 56050 frames Cell Events: 2932 mean_circ: 0.933492
Ran for 57050 frames Cell Events: 2981 mean_circ: 0.933601
Ran for 57550 frames Cell Events: 3004 mean_circ: 0.933638
Ran for 58050 frames Cell Events: 3024 mean_circ: 0.933653
Ran for 61050 frames Cell Events: 3211 mean_circ: 0.933448
Ran for 62050 frames Cell Events: 3262 mean_circ: 0.933347
Ran for 62550 frames Cell Events: 3294 mean_circ: 0.933416
Ran for 63550 frames Cell Events: 3351 mean_circ: 0.933531
Ran for 64050 frames Cell Events: 3376 mean_circ: 0.933523
Ran for 64550 frames Cell Events: 3408 mean_circ: 0.93355
Ran for 65050 frames Cell Events: 3431 mean_circ: 0.933581
Ran for 65550 frames Cell Events: 3450 mean_circ: 0.933648
Ran for 66050 frames Cell Events: 3474 mean_circ: 0.933604
Ran for 66550 frames Cell Events: 3499 mean_circ: 0.933613
Ran for 68550 frames Cell Events: 3616 mean_circ: 0.933511
Ran for 69050 frames Cell Events: 3638 mean_circ: 0.933518
Ran for 70050 frames Cell Events: 3684 mean_circ: 0.933421
Ran for 73050 frames Cell Events: 3846 mean_circ: 0.933586
Ran for 73550 frames Cell Events: 3868 mean_circ: 0.933597
Ran for 74050 frames Cell Events: 3894 mean_circ: 0.933677
Ran for 74550 frames Cell Events: 3916 mean_circ: 0.933736
Ran for 75050 frames Cell Events: 3944 mean_circ: 0.933804
Ran for 76550 frames Cell Events: 4025 mean_circ: 0.933905
Ran for 77050 frames Cell Events: 4053 mean_circ: 0.933997
Ran for 79050 frames Cell Events: 4176 mean_circ: 0.93404
Ran for 79550 frames Cell Events: 4204 mean_circ: 0.933905
Ran for 80050 frames Cell Events: 4234 mean_circ: 0.933908
Ran for 80550 frames Cell Events: 4265 mean_circ: 0.933934
Ran for 81550 frames Cell Events: 4324 mean_circ: 0.933909
Ran for 82050 frames Cell Events: 4337 mean_circ: 0.933893
Ran for 83050 frames Cell Events: 4387 mean_circ: 0.933922
Ran for 83550 frames Cell Events: 4422 mean_circ: 0.933932
Ran for 84050 frames Cell Events: 4442 mean_circ: 0.933887
Ran for 85050 frames Cell Events: 4505 mean_circ: 0.933927
Ran for 85550 frames Cell Events: 4535 mean_circ: 0.93402
Ran for 86550 frames Cell Events: 4565 mean_circ: 0.93398
Ran for 87550 frames Cell Events: 4609 mean_circ: 0.933814
Ran for 88550 frames Cell Events: 4661 mean_circ: 0.933736
Ran for 89050 frames Cell Events: 4691 mean_circ: 0.933822
Ran for 89550 frames Cell Events: 4711 mean_circ: 0.933835
Ran for 90050 frames Cell Events: 4735 mean_circ: 0.933796
Ran for 90550 frames Cell Events: 4761 mean_circ: 0.933885
Ran for 91050 frames Cell Events: 4792 mean_circ: 0.933852
Ran for 91550 frames Cell Events: 4815 mean_circ: 0.933789
Ran for 92050 frames Cell Events: 4855 mean_circ: 0.933789
Ran for 93550 frames Cell Events: 4941 mean_circ: 0.933871
Ran for 94550 frames Cell Events: 5007 mean_circ: 0.933756
Ran for 95050 frames Cell Events: 5040 mean_circ: 0.933808
Ran for 96050 frames Cell Events: 5096 mean_circ: 0.933943
Ran for 97550 frames Cell Events: 5164 mean_circ: 0.933886
Ran for 98050 frames Cell Events: 5190 mean_circ: 0.933878
Ran for 99050 frames Cell Events: 5249 mean_circ: 0.933845
Ran for 99550 frames Cell Events: 5275 mean_circ: 0.933834
Ran for 100050 frames Cell Events: 5304 mean_circ: 0.933818
Ran for 101550 frames Cell Events: 5396 mean_circ: 0.933691
Ran for 102050 frames Cell Events: 5417 mean_circ: 0.933688
Ran for 103050 frames Cell Events: 5459 mean_circ: 0.933673
Ran for 104050 frames Cell Events: 5531 mean_circ: 0.933594
Ran for 104550 frames Cell Events: 5570 mean_circ: 0.933638
Ran for 105050 frames Cell Events: 5598 mean_circ: 0.933688
Ran for 106550 frames Cell Events: 5693 mean_circ: 0.933733
Ran for 107050 frames Cell Events: 5722 mean_circ: 0.933711
Ran for 107550 frames Cell Events: 5746 mean_circ: 0.93362
Ran for 108050 frames Cell Events: 5772 mean_circ: 0.933565
Ran for 108550 frames Cell Events: 5792 mean_circ: 0.933548
Ran for 110050 frames Cell Events: 5870 mean_circ: 0.933452
Ran for 110550 frames Cell Events: 5898 mean_circ: 0.933478
Ran for 111050 frames Cell Events: 5924 mean_circ: 0.933515
Ran for 111550 frames Cell Events: 5952 mean_circ: 0.933528
Ran for 112050 frames Cell Events: 5978 mean_circ: 0.933547
Ran for 113050 frames Cell Events: 6028 mean_circ: 0.933459
Ran for 113550 frames Cell Events: 6044 mean_circ: 0.93345
Ran for 114550 frames Cell Events: 6096 mean_circ: 0.933398
Ran for 115050 frames Cell Events: 6123 mean_circ: 0.933389
Ran for 115550 frames Cell Events: 6140 mean_circ: 0.933405
Ran for 116050 frames Cell Events: 6169 mean_circ: 0.933446
Ran for 117050 frames Cell Events: 6226 mean_circ: 0.933491
Ran for 117550 frames Cell Events: 6248 mean_circ: 0.933522
Ran for 118550 frames Cell Events: 6309 mean_circ: 0.933554
Ran for 119550 frames Cell Events: 6333 mean_circ: 0.933553
Ran for 120050 frames Cell Events: 6358 mean_circ: 0.933615
Ran for 121050 frames Cell Events: 6411 mean_circ: 0.933654
Ran for 121550 frames Cell Events: 6438 mean_circ: 0.933729
Ran for 122050 frames Cell Events: 6462 mean_circ: 0.933749
Ran for 122550 frames Cell Events: 6486 mean_circ: 0.933768
Ran for 126050 frames Cell Events: 6703 mean_circ: 0.933669
Ran for 126550 frames Cell Events: 6723 mean_circ: 0.933656
Ran for 128050 frames Cell Events: 6825 mean_circ: 0.933547
Ran for 128550 frames Cell Events: 6849 mean_circ: 0.933539
Ran for 131050 frames Cell Events: 6982 mean_circ: 0.933639
Ran for 131550 frames Cell Events: 7007 mean_circ: 0.933625
Ran for 134050 frames Cell Events: 7163 mean_circ: 0.933825
Ran for 135550 frames Cell Events: 7233 mean_circ: 0.93388
Ran for 137550 frames Cell Events: 7337 mean_circ: 0.933919
Ran for 140050 frames Cell Events: 7511 mean_circ: 0.933973
Ran for 142050 frames Cell Events: 7630 mean_circ: 0.933977
Ran for 142550 frames Cell Events: 7663 mean_circ: 0.934
Ran for 143050 frames Cell Events: 7700 mean_circ: 0.933997
Ran for 145050 frames Cell Events: 7825 mean_circ: 0.934045
Ran for 147050 frames Cell Events: 7905 mean_circ: 0.934064
Ran for 148550 frames Cell Events: 7979 mean_circ: 0.934116
Ran for 149050 frames Cell Events: 8009 mean_circ: 0.934179
Ran for 150550 frames Cell Events: 8078 mean_circ: 0.934229
Ran for 151050 frames Cell Events: 8110 mean_circ: 0.934232
Ran for 151550 frames Cell Events: 8133 mean_circ: 0.934268
End this measurment..
Stopping time: 1.32424e+009
****************************************
Measurement finished
Mean Circularitiy: 0.934248
Mean Area: 591.007
Mean FL-1 intensity: 2584.22
Mean FL-2 intensity: 3834.23
Mean FL-3 intensity: 0
frames processed: 152489
Total Time: 76732.6ms
Number of Runs (new bg img) 0
Frames collected: 153292
Skipped frames: 46701
Rescued Frames: 2312463
Samples DAQ: 38323051
Samples written: 76646018
Samples per Channel: 38323009
FPS: 1997.74
Samples per second: 499436
Expected samples/s: 500000
Events: 8230
Events per second: 107.256
jumped back (PMT): 14
#Frames read, frequency
# 0 frames :232457
# 1 frames :60519
# 2 frames :3496
# 3 frames :1129
# 4 frames :627
# 5 frames :284
# 6 frames :1779
# 7 frames :1377
# 8 frames :996
# 9 frames :582
# 10 frames :694
# 11 frames :491
# 12 frames :382
# 13 frames :204
# 14 frames :206
# 15 frames :195
# 16 frames :173
# 17 frames :127
# 18 frames :95
# 19 frames :78
# 20 frames :54
# 21 frames :62
# 22 frames :56
# 23 frames :41
# 24 frames :38
# 25 frames :29
# 26 frames :31
# 27 frames :19
# 28 frames :12
# 29 frames :13
# 30 frames :9
# 31 frames :11
# 32 frames :6
# 33 frames :9
# 34 frames :4
# 35 frames :7
# 37 frames :4
# 38 frames :1
# 39 frames :4
# 40 frames :1
# 42 frames :2
# 43 frames :1
# 44 frames :1
# 45 frames :1
# 46 frames :1
# 47 frames :3
# 48 frames :1
# 49 frames :1
# 50 frames :1
# 55 frames :1
# 56 frames :1
# 58 frames :1
# 83 frames :1
M1_para.ini
[General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100
[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0
#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 24.9048
parameters.txt
#fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0