Click on the log titles below to expand the log entries present in this DC resource.
dclab-export_2024-07-10_11.41.49
{"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc
{
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "c669dad028e81edeff446bc4c67d4f0f",
"name": "M1_20170530_1638_dsRNA_I8_col.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "09:42:11"
}
}
dclab-tdms2rtdc-warnings
LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8032 (max 8033) in '8032'.
FG_Config.mcf
[Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;
[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;
[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini
[Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6
[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini
[EVENT LOG] fRT-DC V1.06170206
Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 24.951
Start pulse train for BG Aquisition..
Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 1.32571e+009
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 1050 frames Cell Events: 34 mean_circ: 0.913909
Ran for 1550 frames Cell Events: 44 mean_circ: 0.913421
Ran for 2050 frames Cell Events: 58 mean_circ: 0.915308
Ran for 2550 frames Cell Events: 78 mean_circ: 0.916871
Ran for 5050 frames Cell Events: 151 mean_circ: 0.921224
Ran for 5550 frames Cell Events: 177 mean_circ: 0.92077
Ran for 6550 frames Cell Events: 218 mean_circ: 0.924618
Ran for 7050 frames Cell Events: 231 mean_circ: 0.924218
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Ran for 8550 frames Cell Events: 273 mean_circ: 0.925147
Ran for 9050 frames Cell Events: 291 mean_circ: 0.925791
Ran for 10550 frames Cell Events: 335 mean_circ: 0.927465
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Ran for 12050 frames Cell Events: 378 mean_circ: 0.926603
Ran for 13050 frames Cell Events: 391 mean_circ: 0.927128
Ran for 13550 frames Cell Events: 404 mean_circ: 0.926904
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Ran for 56550 frames Cell Events: 2023 mean_circ: 0.929364
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Ran for 74050 frames Cell Events: 2631 mean_circ: 0.928335
Ran for 74550 frames Cell Events: 2655 mean_circ: 0.928397
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Ran for 80050 frames Cell Events: 2869 mean_circ: 0.928669
Ran for 80550 frames Cell Events: 2887 mean_circ: 0.928484
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Ran for 81550 frames Cell Events: 2933 mean_circ: 0.928273
Ran for 82550 frames Cell Events: 2965 mean_circ: 0.928327
Ran for 83550 frames Cell Events: 3006 mean_circ: 0.928331
Ran for 85050 frames Cell Events: 3059 mean_circ: 0.928275
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Ran for 91050 frames Cell Events: 3298 mean_circ: 0.928309
Ran for 91550 frames Cell Events: 3313 mean_circ: 0.928345
Ran for 92050 frames Cell Events: 3334 mean_circ: 0.928329
Ran for 93050 frames Cell Events: 3358 mean_circ: 0.928307
Ran for 93550 frames Cell Events: 3378 mean_circ: 0.928325
Ran for 94550 frames Cell Events: 3415 mean_circ: 0.928272
Ran for 95050 frames Cell Events: 3427 mean_circ: 0.928356
Ran for 95550 frames Cell Events: 3450 mean_circ: 0.928438
Ran for 96050 frames Cell Events: 3463 mean_circ: 0.928444
Ran for 97050 frames Cell Events: 3506 mean_circ: 0.928431
Ran for 98550 frames Cell Events: 3567 mean_circ: 0.928229
Ran for 99050 frames Cell Events: 3579 mean_circ: 0.928274
Ran for 99550 frames Cell Events: 3596 mean_circ: 0.928256
Ran for 100550 frames Cell Events: 3632 mean_circ: 0.928337
Ran for 101050 frames Cell Events: 3647 mean_circ: 0.928443
Ran for 101550 frames Cell Events: 3665 mean_circ: 0.928484
Ran for 102550 frames Cell Events: 3703 mean_circ: 0.928413
Ran for 103550 frames Cell Events: 3738 mean_circ: 0.928309
Ran for 104050 frames Cell Events: 3752 mean_circ: 0.928328
Ran for 104550 frames Cell Events: 3766 mean_circ: 0.928314
Ran for 105050 frames Cell Events: 3778 mean_circ: 0.928309
Ran for 107050 frames Cell Events: 3846 mean_circ: 0.928147
Ran for 107550 frames Cell Events: 3856 mean_circ: 0.928205
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Ran for 120550 frames Cell Events: 4292 mean_circ: 0.92799
Ran for 121050 frames Cell Events: 4310 mean_circ: 0.927994
Ran for 121550 frames Cell Events: 4323 mean_circ: 0.928016
Ran for 122050 frames Cell Events: 4338 mean_circ: 0.927961
Ran for 122550 frames Cell Events: 4359 mean_circ: 0.927884
Ran for 123050 frames Cell Events: 4373 mean_circ: 0.927833
Ran for 123550 frames Cell Events: 4387 mean_circ: 0.927846
Ran for 124550 frames Cell Events: 4423 mean_circ: 0.927905
Ran for 125050 frames Cell Events: 4437 mean_circ: 0.92796
Ran for 125550 frames Cell Events: 4448 mean_circ: 0.928006
Ran for 126050 frames Cell Events: 4471 mean_circ: 0.928027
Ran for 126550 frames Cell Events: 4493 mean_circ: 0.928011
Ran for 127050 frames Cell Events: 4511 mean_circ: 0.928001
Ran for 128050 frames Cell Events: 4539 mean_circ: 0.928003
Ran for 129550 frames Cell Events: 4587 mean_circ: 0.928094
Ran for 130050 frames Cell Events: 4606 mean_circ: 0.928141
Ran for 130550 frames Cell Events: 4626 mean_circ: 0.928071
Ran for 131550 frames Cell Events: 4659 mean_circ: 0.928121
Ran for 132050 frames Cell Events: 4677 mean_circ: 0.928184
Ran for 132550 frames Cell Events: 4691 mean_circ: 0.928064
Ran for 133050 frames Cell Events: 4702 mean_circ: 0.928091
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Ran for 136050 frames Cell Events: 4805 mean_circ: 0.927996
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Ran for 142550 frames Cell Events: 5022 mean_circ: 0.928045
Ran for 143050 frames Cell Events: 5048 mean_circ: 0.928062
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Ran for 144050 frames Cell Events: 5076 mean_circ: 0.928007
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Ran for 147050 frames Cell Events: 5179 mean_circ: 0.928054
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Ran for 171550 frames Cell Events: 6031 mean_circ: 0.927967
Ran for 172550 frames Cell Events: 6054 mean_circ: 0.927979
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Ran for 176050 frames Cell Events: 6174 mean_circ: 0.928079
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Ran for 181550 frames Cell Events: 6346 mean_circ: 0.928079
Ran for 182050 frames Cell Events: 6358 mean_circ: 0.928085
Ran for 182550 frames Cell Events: 6373 mean_circ: 0.928099
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Ran for 192050 frames Cell Events: 6716 mean_circ: 0.928137
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Ran for 193050 frames Cell Events: 6743 mean_circ: 0.928163
Ran for 193550 frames Cell Events: 6757 mean_circ: 0.928141
Ran for 194050 frames Cell Events: 6781 mean_circ: 0.928112
Ran for 195050 frames Cell Events: 6835 mean_circ: 0.928057
Ran for 196050 frames Cell Events: 6882 mean_circ: 0.928012
Ran for 198050 frames Cell Events: 6968 mean_circ: 0.928066
Ran for 198550 frames Cell Events: 6980 mean_circ: 0.928049
Ran for 199050 frames Cell Events: 6991 mean_circ: 0.928084
Ran for 199550 frames Cell Events: 7015 mean_circ: 0.928042
Ran for 200050 frames Cell Events: 7037 mean_circ: 0.928067
Ran for 200550 frames Cell Events: 7062 mean_circ: 0.928025
Ran for 201550 frames Cell Events: 7082 mean_circ: 0.928056
Ran for 203550 frames Cell Events: 7155 mean_circ: 0.92807
Ran for 204050 frames Cell Events: 7169 mean_circ: 0.928056
Ran for 206050 frames Cell Events: 7246 mean_circ: 0.927954
Ran for 207050 frames Cell Events: 7284 mean_circ: 0.927982
Ran for 207550 frames Cell Events: 7293 mean_circ: 0.928006
Ran for 208050 frames Cell Events: 7314 mean_circ: 0.927964
Ran for 208550 frames Cell Events: 7331 mean_circ: 0.927999
Ran for 209050 frames Cell Events: 7352 mean_circ: 0.92801
Ran for 209550 frames Cell Events: 7368 mean_circ: 0.928059
Ran for 210550 frames Cell Events: 7398 mean_circ: 0.928068
Ran for 211050 frames Cell Events: 7421 mean_circ: 0.928084
Ran for 211550 frames Cell Events: 7440 mean_circ: 0.92808
Ran for 212050 frames Cell Events: 7475 mean_circ: 0.928064
Ran for 213550 frames Cell Events: 7540 mean_circ: 0.928048
Ran for 214050 frames Cell Events: 7552 mean_circ: 0.928074
Ran for 214550 frames Cell Events: 7567 mean_circ: 0.92809
Ran for 215050 frames Cell Events: 7581 mean_circ: 0.928102
Ran for 216050 frames Cell Events: 7620 mean_circ: 0.928132
Ran for 216550 frames Cell Events: 7634 mean_circ: 0.92818
Ran for 217050 frames Cell Events: 7654 mean_circ: 0.928206
Ran for 217550 frames Cell Events: 7672 mean_circ: 0.928248
Ran for 218050 frames Cell Events: 7700 mean_circ: 0.928157
Ran for 219550 frames Cell Events: 7753 mean_circ: 0.928186
Ran for 220050 frames Cell Events: 7789 mean_circ: 0.928187
Ran for 221050 frames Cell Events: 7835 mean_circ: 0.92814
Ran for 222050 frames Cell Events: 7877 mean_circ: 0.928081
Ran for 223050 frames Cell Events: 7907 mean_circ: 0.928126
Ran for 223550 frames Cell Events: 7931 mean_circ: 0.928122
Ran for 224550 frames Cell Events: 7961 mean_circ: 0.92809
Ran for 225550 frames Cell Events: 7983 mean_circ: 0.928124
Ran for 226050 frames Cell Events: 8003 mean_circ: 0.928175
End this measurment..
Stopping time: 1.32582e+009
****************************************
Measurement finished
Mean Circularitiy: 0.928226
Mean Area: 659.478
Mean FL-1 intensity: 2587.41
Mean FL-2 intensity: 3506.57
Mean FL-3 intensity: 0
frames processed: 224639
Total Time: 113511ms
Number of Runs (new bg img) 0
Frames collected: 226846
Skipped frames: 52687
Rescued Frames: 2599265
Samples DAQ: 56712578
Samples written: 113424048
Samples per Channel: 56712024
FPS: 1998.44
Samples per second: 499615
Expected samples/s: 500000
Events: 8033
Events per second: 70.7682
jumped back (PMT): 5779
#Frames read, frequency
# 0 frames :310070
# 1 frames :126766
# 2 frames :5308
# 3 frames :1473
# 4 frames :839
# 5 frames :292
# 6 frames :2540
# 7 frames :2070
# 8 frames :1249
# 9 frames :624
# 10 frames :604
# 11 frames :364
# 12 frames :365
# 13 frames :218
# 14 frames :200
# 15 frames :164
# 16 frames :133
# 17 frames :85
# 18 frames :70
# 19 frames :31
# 20 frames :34
# 21 frames :27
# 22 frames :32
# 23 frames :22
# 24 frames :17
# 25 frames :12
# 26 frames :7
# 27 frames :8
# 28 frames :7
# 29 frames :2
# 30 frames :1
# 31 frames :7
# 32 frames :6
# 33 frames :4
# 34 frames :1
# 35 frames :1
# 36 frames :2
# 39 frames :1
# 43 frames :2
# 44 frames :1
# 46 frames :1
# 51 frames :1
# 58 frames :1
# 76 frames :1
M1_para.ini
[General]
Measurement Number = 1
Flow Rate [ul/s] = 0.04
Region = "Channel"
Channel width = 20
Current LED = 100
[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0
#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 24.951
parameters.txt
#fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 300
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0