Click on the log titles below to expand the log entries present in this DC resource.
dclab-export_2024-07-10_11.44.21
{"dclab version": [0, 60, 3], "kwargs": {"features": ["area_cvx", "area_msd", "circ", "contour", "fl1_area", "fl1_dist", "fl1_max", "fl1_npeaks", "fl1_pos", "fl1_width", "fl2_area", "fl2_dist", "fl2_max", "fl2_npeaks", "fl2_pos", "fl2_width", "frame", "image", "inert_ratio_cvx", "inert_ratio_raw", "mask", "nevents", "pos_x", "pos_y", "size_x", "size_y", "trace"], "filtered": true, "logs": false, "tables": false, "basins": false, "meta_prefix": "src_", "skip_checks": false}}
dclab-tdms2rtdc
{
"ancillary features": [
"mask"
],
"files": [
{
"index": 1,
"md5-5M": "b7ace266f73dd6b1f662712d56684371",
"name": "M1_20170530_1709_dsRNA_J8_faster.tdms"
}
],
"libraries": {
"dclab": "0.60.3",
"h5py": "3.11.0",
"imageio": "2.34.2",
"nptdms": "1.8.0",
"numpy": "1.26.4"
},
"python": {
"build": "tags/v3.11.1:a7a450f, Dec 6 2022 19:58:39",
"implementation": "CPython",
"version": "3.11.1"
},
"system": {
"info": "Windows-10-10.0.19045-SP0",
"machine": "AMD64",
"name": "Windows",
"release": "10",
"version": "10.0.19045"
},
"utc": {
"date": "2024-07-10",
"time": "09:44:42"
}
}
dclab-tdms2rtdc-warnings
LimitingExportSizeWarning
in dclab.rtdc_dataset.export line 305:
Not all features have the same length! Limiting output event count to 8030 (max 8031) in '8030'.
FG_Config.mcf
[Kamerasektion]
ConfigFileVersion=2.0;
Hersteller='Generic-CameraLink';
Typ='vas';
HapName='C:/Lib/SiliconSoftware/Runtime5.4.1/Hardware Applets/mE4VD4-CL/PR_BG_subtr_141204_ext_trig_noerode.hap';
Version='1.5';
LoadAppletId=0;
AktivePort=0;
[ID0]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
FG_PORT=0;
FG_GLOBAL_ACCESS=0;
Device1_Process0_UpperBits_BitOffset=4;
Device1_Process0_module9_SyncMode=0;
Device1_Process0_Camera_DvalMode=1;
Device1_Process0_Camera_FullMode=0;
Device1_Process0_DMA_PixelsPerLine=0;
Device1_Process0_DMA_BytesPerLine=0;
Device1_Process0_LowerBits_BitOffset=0;
Device1_Process0_Buffer1_XOffset=0;
Device1_Process0_Buffer1_XLength=256;
Device1_Process0_Buffer1_YOffset=0;
Device1_Process0_Buffer1_YLength=96;
Device1_Process0_Buffer1_FillLevel=75;
Device1_Process0_Buffer1_Overflow=0;
Device1_Process0_Buffer1_InfiniteSource=0;
Device1_Process0_Buffer0_XOffset=0;
Device1_Process0_Buffer0_XLength=256;
Device1_Process0_Buffer0_YOffset=0;
Device1_Process0_Buffer0_YLength=96;
Device1_Process0_Buffer0_FillLevel=75;
Device1_Process0_Buffer0_Overflow=0;
Device1_Process0_Buffer0_InfiniteSource=0;
Device1_Process0_module12_BufferWidth=400;
Device1_Process0_module12_BufferHeight=96;
Device1_Process0_module12_XOffset=0;
Device1_Process0_module12_XLength=256;
Device1_Process0_module12_YOffset=0;
Device1_Process0_module12_YLength=96;
Device1_Process0_module12_UpdateROI=1;
Device1_Process0_module12_CoefficientFile 0='coefficients_0.tif';
Device1_Process0_module12_LoadCoefficients=1;
Device1_Process0_module13_SyncMode=0;
Device1_Process0_module16_from=18446744073709551360;
Device1_Process0_module16_to=18446744073709551610;
Device1_Process0_module17_Value=0;
Device1_Process0_module20_PixelsPerLine=0;
Device1_Process0_module20_BytesPerLine=0;
Device1_Process0_module21_Number=0;
Device1_Process0_module26_ScaleFactorMaxBits=8;
Device1_Process0_module26_ScaleFactor=255;
Device1_Process0_module28_TriggerMode=2;
Device1_Process0_module28_ExsyncEnable=1;
Device1_Process0_module28_FlashEnable=1;
Device1_Process0_module28_SoftwareTrgPulse=1;
Device1_Process0_module28_SoftwareTrgIsBusy=0;
Device1_Process0_module28_ImgTrgInSource=0;
Device1_Process0_module28_ImgTrgInPolarity=0;
Device1_Process0_module28_ImgTrgDownscale=1;
Device1_Process0_module28_Accuracy=2;
Device1_Process0_module28_ExsyncFramesPerSec=2000;
Device1_Process0_module28_ExsyncExposure=20;
Device1_Process0_module28_ExsyncDelay=0;
Device1_Process0_module28_ExsyncPolarity=0;
Device1_Process0_module28_FlashDelay=0;
Device1_Process0_module28_SoftwareTrgDeadTime=10000;
Device1_Process0_module28_FlashPolarity=1;
Device1_Process0_module28_CC1output=0;
Device1_Process0_module28_CC2output=8;
Device1_Process0_module28_CC3output=8;
Device1_Process0_module28_CC4output=8;
Device1_Process0_module28_DebouncingMaxTime=65.52;
Device1_Process0_module28_DebouncingTime=0.112;
[ID1]
FG_WIDTH=256;
FG_HEIGHT=96;
FG_TIMEOUT=1000000;
FG_FORMAT=3;
M1_camera.ini
[Framerate]
Shutter Time = 20
Frame Rate = 2000
Tap Mode = 8x8 Bit
Tap Code = 6
[ROI]
x-pos = 504
y-pos = 472
width = 256
height = 96
M1_log.ini
[EVENT LOG] fRT-DC V1.06170206
Reset global variables..
Circular Buffer Size is: 1000000
Ambient Temperature [C] = 24.9981
Start pulse train for BG Aquisition..
Now refreshing the background image stored in the framegrabber
STATE: 3
Starting time: 1.32762e+009
Use analog input: pci/ai4,pci/ai5
Samplerate per channel: 500000
Buffersize in byte: 1000000
Rising Edge, Continuous Sampling
N_callback: 125
Callbacks per frame: 2
Callback registered
Configure tasks for online measurement..
Timing: start directly
Freq: 2000
Duty cycle: 0.5
Reset global variables: i0, i1, samplesThisRun
Start the Fragegrabber ..
Start the NI-DAQ Card..
Stop after time: 0
Stop after nr. of cells: 10000
Ran for 550 frames Cell Events: 24 mean_circ: 0.923148
Ran for 1050 frames Cell Events: 50 mean_circ: 0.92558
Ran for 1550 frames Cell Events: 70 mean_circ: 0.924686
Ran for 2050 frames Cell Events: 91 mean_circ: 0.924175
Ran for 2550 frames Cell Events: 109 mean_circ: 0.927611
Ran for 3050 frames Cell Events: 127 mean_circ: 0.927715
Ran for 4050 frames Cell Events: 150 mean_circ: 0.930052
Ran for 4550 frames Cell Events: 177 mean_circ: 0.931605
Ran for 5050 frames Cell Events: 200 mean_circ: 0.932203
Ran for 5550 frames Cell Events: 214 mean_circ: 0.930975
Ran for 6050 frames Cell Events: 235 mean_circ: 0.931438
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Ran for 7550 frames Cell Events: 292 mean_circ: 0.931972
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Ran for 10050 frames Cell Events: 406 mean_circ: 0.931135
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Ran for 63550 frames Cell Events: 2418 mean_circ: 0.929937
Ran for 64050 frames Cell Events: 2434 mean_circ: 0.929977
Ran for 64550 frames Cell Events: 2454 mean_circ: 0.930106
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Ran for 71550 frames Cell Events: 2760 mean_circ: 0.930287
Ran for 73050 frames Cell Events: 2826 mean_circ: 0.930213
Ran for 73550 frames Cell Events: 2842 mean_circ: 0.930219
Ran for 74550 frames Cell Events: 2879 mean_circ: 0.930181
Ran for 75550 frames Cell Events: 2916 mean_circ: 0.930035
Ran for 76050 frames Cell Events: 2942 mean_circ: 0.929992
Ran for 78050 frames Cell Events: 3020 mean_circ: 0.930094
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Ran for 79050 frames Cell Events: 3070 mean_circ: 0.930109
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Ran for 80050 frames Cell Events: 3111 mean_circ: 0.930064
Ran for 81550 frames Cell Events: 3180 mean_circ: 0.930113
Ran for 82050 frames Cell Events: 3188 mean_circ: 0.930161
Ran for 82550 frames Cell Events: 3205 mean_circ: 0.930169
Ran for 83050 frames Cell Events: 3230 mean_circ: 0.930122
Ran for 84050 frames Cell Events: 3275 mean_circ: 0.930018
Ran for 84550 frames Cell Events: 3290 mean_circ: 0.929954
Ran for 85050 frames Cell Events: 3311 mean_circ: 0.929957
Ran for 85550 frames Cell Events: 3332 mean_circ: 0.930067
Ran for 87050 frames Cell Events: 3383 mean_circ: 0.930108
Ran for 87550 frames Cell Events: 3407 mean_circ: 0.929929
Ran for 88050 frames Cell Events: 3425 mean_circ: 0.929925
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Ran for 90050 frames Cell Events: 3501 mean_circ: 0.92982
Ran for 91550 frames Cell Events: 3564 mean_circ: 0.929847
Ran for 92050 frames Cell Events: 3590 mean_circ: 0.929941
Ran for 94050 frames Cell Events: 3687 mean_circ: 0.929914
Ran for 94550 frames Cell Events: 3701 mean_circ: 0.929954
Ran for 95550 frames Cell Events: 3735 mean_circ: 0.929985
Ran for 96050 frames Cell Events: 3751 mean_circ: 0.929935
Ran for 96550 frames Cell Events: 3772 mean_circ: 0.929929
Ran for 97050 frames Cell Events: 3799 mean_circ: 0.929866
Ran for 97550 frames Cell Events: 3823 mean_circ: 0.929754
Ran for 98050 frames Cell Events: 3847 mean_circ: 0.929681
Ran for 99050 frames Cell Events: 3884 mean_circ: 0.929596
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Ran for 100050 frames Cell Events: 3931 mean_circ: 0.929611
Ran for 100550 frames Cell Events: 3956 mean_circ: 0.929513
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Ran for 101550 frames Cell Events: 3997 mean_circ: 0.929466
Ran for 102050 frames Cell Events: 4018 mean_circ: 0.929516
Ran for 102550 frames Cell Events: 4042 mean_circ: 0.929592
Ran for 103050 frames Cell Events: 4070 mean_circ: 0.929678
Ran for 103550 frames Cell Events: 4097 mean_circ: 0.929703
Ran for 104050 frames Cell Events: 4114 mean_circ: 0.929662
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Ran for 105550 frames Cell Events: 4202 mean_circ: 0.929591
Ran for 106050 frames Cell Events: 4225 mean_circ: 0.929582
Ran for 106550 frames Cell Events: 4252 mean_circ: 0.929572
Ran for 107050 frames Cell Events: 4271 mean_circ: 0.929487
Ran for 107550 frames Cell Events: 4293 mean_circ: 0.929463
Ran for 110050 frames Cell Events: 4408 mean_circ: 0.929412
Ran for 111050 frames Cell Events: 4452 mean_circ: 0.92948
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Ran for 112050 frames Cell Events: 4486 mean_circ: 0.929501
Ran for 112550 frames Cell Events: 4510 mean_circ: 0.929509
Ran for 114050 frames Cell Events: 4578 mean_circ: 0.929508
Ran for 115550 frames Cell Events: 4646 mean_circ: 0.929541
Ran for 116050 frames Cell Events: 4666 mean_circ: 0.929528
Ran for 116550 frames Cell Events: 4682 mean_circ: 0.929563
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Ran for 120050 frames Cell Events: 4835 mean_circ: 0.929503
Ran for 120550 frames Cell Events: 4850 mean_circ: 0.929445
Ran for 122050 frames Cell Events: 4912 mean_circ: 0.929528
Ran for 122550 frames Cell Events: 4933 mean_circ: 0.929531
Ran for 123050 frames Cell Events: 4952 mean_circ: 0.929449
Ran for 123550 frames Cell Events: 4966 mean_circ: 0.92942
Ran for 124050 frames Cell Events: 4981 mean_circ: 0.92943
Ran for 126050 frames Cell Events: 5048 mean_circ: 0.929438
Ran for 127050 frames Cell Events: 5093 mean_circ: 0.929416
Ran for 127550 frames Cell Events: 5108 mean_circ: 0.929462
Ran for 128050 frames Cell Events: 5127 mean_circ: 0.929464
Ran for 128550 frames Cell Events: 5155 mean_circ: 0.929521
Ran for 129050 frames Cell Events: 5176 mean_circ: 0.929544
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Ran for 130550 frames Cell Events: 5234 mean_circ: 0.92953
Ran for 131050 frames Cell Events: 5247 mean_circ: 0.929579
Ran for 131550 frames Cell Events: 5265 mean_circ: 0.929605
Ran for 133050 frames Cell Events: 5325 mean_circ: 0.929561
Ran for 133550 frames Cell Events: 5344 mean_circ: 0.929514
Ran for 135050 frames Cell Events: 5419 mean_circ: 0.929441
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Ran for 141050 frames Cell Events: 5650 mean_circ: 0.929525
Ran for 143550 frames Cell Events: 5767 mean_circ: 0.929422
Ran for 144550 frames Cell Events: 5812 mean_circ: 0.929436
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Ran for 146550 frames Cell Events: 5877 mean_circ: 0.929366
Ran for 147550 frames Cell Events: 5916 mean_circ: 0.929426
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Ran for 172550 frames Cell Events: 6882 mean_circ: 0.929768
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Ran for 174050 frames Cell Events: 6944 mean_circ: 0.929735
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Ran for 192050 frames Cell Events: 7714 mean_circ: 0.929475
Ran for 193050 frames Cell Events: 7750 mean_circ: 0.929469
Ran for 193550 frames Cell Events: 7769 mean_circ: 0.929469
Ran for 194050 frames Cell Events: 7785 mean_circ: 0.929431
Ran for 195050 frames Cell Events: 7827 mean_circ: 0.929315
Ran for 195550 frames Cell Events: 7853 mean_circ: 0.929338
Ran for 197050 frames Cell Events: 7919 mean_circ: 0.929353
Ran for 197550 frames Cell Events: 7933 mean_circ: 0.92934
Ran for 198550 frames Cell Events: 7982 mean_circ: 0.929339
Ran for 199550 frames Cell Events: 8024 mean_circ: 0.929273
End this measurment..
Stopping time: 1.32772e+009
****************************************
Measurement finished
Mean Circularitiy: 0.929289
Mean Area: 615.201
Mean FL-1 intensity: 2404.24
Mean FL-2 intensity: 3783.85
Mean FL-3 intensity: 0
frames processed: 197637
Total Time: 99940.7ms
Number of Runs (new bg img) 0
Frames collected: 199713
Skipped frames: 63503
Rescued Frames: 3010894
Samples DAQ: 49928708
Samples written: 99856474
Samples per Channel: 49928237
FPS: 1998.32
Samples per second: 499579
Expected samples/s: 500000
Events: 8031
Events per second: 80.3577
jumped back (PMT): 13
#Frames read, frequency
# 0 frames :275118
# 1 frames :109100
# 2 frames :4194
# 3 frames :1424
# 4 frames :544
# 5 frames :204
# 6 frames :3474
# 7 frames :1568
# 8 frames :1279
# 9 frames :619
# 10 frames :393
# 11 frames :243
# 12 frames :309
# 13 frames :162
# 14 frames :195
# 15 frames :144
# 16 frames :103
# 17 frames :46
# 18 frames :45
# 19 frames :20
# 20 frames :35
# 21 frames :32
# 22 frames :19
# 23 frames :11
# 24 frames :13
# 25 frames :13
# 26 frames :11
# 27 frames :9
# 28 frames :6
# 29 frames :6
# 30 frames :3
# 31 frames :2
# 32 frames :1
# 33 frames :2
# 34 frames :3
# 35 frames :1
# 36 frames :1
# 37 frames :2
# 38 frames :1
# 45 frames :1
M1_para.ini
[General]
Measurement Number = 1
Flow Rate [ul/s] = 0.06
Region = "Channel"
Channel width = 20
Current LED = 100
[Image]
Setup = Cell_Flow_2 + Fluor
Bin Ops = 5
Blur = 0
Trig Thresh = 50
t1 = -256
t2 = -6
Pix Size = 0.34
Cell Min = 5
Cell Max = 50
Thresh = 6
Diff_Method = 1
Margin = 0
#[FLUOR]
#fRT-DC V1.06170206
#Samplerate = 500000
#Bitdepthraw = 16
#Format = int16
#ADCmin = -1
#ADCmax = 1
#FL-Channels = 2
Laser Power 488 [mW] = 10
Laser Power 561 [mW] = 35
Laser Power 640 [mW] = 0
Ambient Temp. [C] = 24.9981
parameters.txt
#fRT-DC Configuration file
# You may change the values here before a measurement.
debug 0
hull 1
x_size 256
y_size 96
x_pos 504
y_pos 464
pix_size 0.34
cell_min 5
cell_max_x 50
cell_max_y 50
cell_aspect_min 0
cell_aspect_max 10
threshold1 -256
threshold2 -6
trig_threshold 50
MARGIN 0
write_videos 0
write_phot_det 0
show_video 1
write_events 1
fps 2000
samplesperframe 200
Vmin -1
Vmax 1
invertCh1 1
invertCh2 1
invertCh3 1
invertChX 1
samplerate 500000
bg_img 100
channel_width 20
current_led 100
pulse_led 2
binary_ops 5
blur 0
median_pmt 5
software_binops 1
flowctrl 0
flowwait 60
numberofchannels 2
firstchannel 4
secondchannel 5
thirdchannel -6
laser488 10
laser561 35
laser640 0
peakcountthreshold 1000
peakDipRatio 0.8
offsetFL1 0
offsetFL2 0
offsetFL3 0
rollingAverage 100
stopCell 10000
stopTime 0
minFL1 400
minFL2 668
minFL3 -32768
maxFL1 32767
maxFL2 32767
maxFL3 32767
setTimingAutomatic 1
plotWidth 0
getBrightness 0
intellPeak 0